Últimos itens adicionados do Acervo: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia

O Inmetro é uma autarquia federal, vinculada ao Ministério do Desenvolvimento, Indústria e Comércio Exterior, que atua como Secretaria Executiva do Conselho Nacional de Metrologia, Normalização e Qualidade Industrial (Conmetro), colegiado interministerial, que é o órgão normativo do Sistema Nacional de Metrologia, Normalização e Qualidade Industrial (Sinmetro).

Página 10 dos resultados de 1444 itens digitais encontrados em 0.002 segundos

‣ Prokaryotic diversity in one of the largest hypersaline coastal lagoons in the world

Clementino, Maysa Beatriz Mandetta; Vieira, Ricardo Pilz; Cardoso, Alexander Machado; Nascimento, Ana Paula Alves do; Silveira, Cynthia Barbosa da; Riva, Thiago Cassago; Gonzalez, Alessandra Silva Morilla; Paranhos, Rodolfo Pinheiro da Rocha; Albano, Rodo
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Artigo de Revista Científica
Português
10 p. : il.; Araruama Lagoon is an environment characterized by high salt concentrations. The low raining and high evaporation rates in this region favored the development of many salty ponds around the lagoon. In order to reveal the microbial composition of this system, we performed a 16S rRNA gene survey. Among archaea, most clones were related to uncultured environmental Euryarchaeota. In lagoon water, we found some clones related to Methanomicrobia and Methanothermococcus groups, while in the saline pond water members related to the genus Haloarcula were detected. Bacterial community was dominated by clones related to Gamma-proteobacteria, Actinobacteria, and Synechococcus in lagoon water, while Salinibacter ruber relatives dominated in saline pond. We also detected the presence of Alpha-proteobacteria, Pseudomonas- like bacteria and Verrucomicrobia. Only representatives of the genus Ralstonia were cosmopolitan, being observed in both systems. The detection of a substantial number of clones related to uncultured archaea and bacteria suggest that the hypersaline waters of Araruama harbor a pool of novel prokaryotic phylotypes, distinct from those observed in other similar systems. We also observed clones related to halophilic genera of cyanobacteria that are specific for each habitat studied. Additionally...

‣ Bacterial communities of the marine sponges Hymeniacidon heliophila and Polymastia janeirensis and their environment in Rio de Janeiro, Brazil

Turque, Aline da Silva; Cardoso, Alexander Machado; Silveira, Cynthia Barbosa da; Vieira, Ricardo Pilz; Freitas, Flávia Alvim Dutra de; Albano, Rodolpho Mattos; Gonzalez, Alessandra Silva Morilla; Paranhos, Rodolfo Pinheiro da Rocha; Muricy, Guilherme Ra
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Artigo de Revista Científica
Português
12 p. : il.; In this study we performed a survey of the bacterial communities associated with the Western Atlantic demosponges Hymeniacidon heliophila and Polymastia janeirensis, based on 16S rRNA sequencing and transmission electron microscopy (TEM). We compared diversity and composition of the sponge-associated bacteria to those of environmental bacteria, represented by free-living bacterioplankton and by bacteria attached to organic particulate matter in superWcial sediments. Partial bacterial 16S rRNA sequences from seawater, sediment, and sponges were retrieved by PCR, cloning, and sequencing. Sequences were subjected to rarefaction analyses, phylogenetic tree construction, and LIBSHUFF quantitative statistics to verify coverage and similarity between libraries. Community structure of the free-living bacterioplankton was phylogenetically different from that of the sponge-associated bacterial assemblages. On the other hand, some sediment-attached bacteria were also found in the sponge bacterial community, indicating that sponges may incorporate bacteria together with sediment particles. Rare and few prokaryotic morphotypes were found in TEM analyses of sponge mesohyl matrix of both species. Molecular data indicate that bacterial richness and diversity decreases from bacterioplankton...

‣ Coastal bacterioplankton community diversity along a latitudinal gradient in Latin America by means of V6 tag pyrosequencing

Thompson, Fabiano Lopes; Rodrigues, Thiago Bruce; Gonzalez, Alessandra Silva Morilla; Cardoso, Alexander Machado; Clementino, Maysa Beatriz Mandetta; Costagliola, Marcela; Hozbor, Constanza; Otero, Ernesto; Piccini, Claudia; Peressutti, Silvia; Schmieder,
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Artigo de Revista Científica
Português
10 p. : il.; The bacterioplankton diversity of coastal waters along a latitudinal gradient between Puerto Rico and Argentina was analyzed using a total of 134,197 high-quality sequences from the V6 hypervariable region of the small-subunit ribosomal RNA gene (16S rRNA) (mean length of 60 nt). Most of the OTUs were identified into Proteobacteria, Bacteriodetes, Cyanobacteria, and Actinobacteria, corresponding to approx. 80% of the total number of sequences. The number of OTUs corresponding to species varied between 937 and 1946 in the seven locations. Proteobacteria appeared at high frequency in the seven locations. An enrichment of Cyanobacteria was observed in Puerto Rico, whereas an enrichment of Bacteroidetes was detected in the Argentinian shelf and Uruguayan coastal lagoons. The highest number of sequences of Actinobacteria and Acidobacteria were obtained in the Amazon estuary mouth. The rarefaction curves and Good coverage estimator for species diversity suggested a significant coverage, with values ranging between 92 and 97% for Good coverage. Conserved taxa corresponded to aprox. 52% of all sequences. This study suggests that human contaminated environments may inXuence bacterioplankton diversity.

‣ Hunting for extremophiles in Rio de Janeiro

Cardoso, Alexander Machado; Vieira, Ricardo Pilz; Paranhos, Rodolfo Pinheiro da Rocha; Clementino, Maysa Beatriz Mandetta; Albano, Rodolpho Mattos; Martins, Orlando Bonifácio
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Artigo de Revista Científica
Português
3 p. : il.; In this Opinion Article, we argue that tropical regions are, in fact, hot spots of extreme microbial diversity. The scope of the topic is vast and while this paper cannot be seen as exhaustive, it will hopefully serve to stimulate research interest in the reader to explore this fascinating area of microbiology and promote knowledge sharing and scientific collaboration. The article discusses the following points: (i) what extremophiles are and how useful they are to biotechnology; (ii) the importance of molecular techniques for extremophile identification due to their unculturability; and (iii) extremophile research performed in areas of different environmental conditions in Rio de Janeiro, Brazil.

‣ Diversity of bacterial communities related to the nitrogen cycle in a coastal tropical bay

Gonzalez, Alessandra Silva Morilla; Vieira, Ricardo Pilz; Cardoso, Alexander Machado; Clementino, Maysa Beatriz Mandetta; Albano, Rodolpho Mattos; Hagler, Leda Cristina Santana Mendonça; Martins, Orlando Bonifácio; Paranhos, Rodolfo Pinheiro da Rocha
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Artigo de Revista Científica
Português
7 p. : il.; A culture-independent molecular phylogenetic analysis was carried out to study for the first time the diversity of bacterial ammonia monooxygenase subunit A (amoA) and nitrogenase reductase subunit H (nifH) genes from Urca inlet at Guanabara Bay in Rio de Janeiro, Brazil. Most bacterial amoA and nifH sequences exhibited identities of less than 95% to those in the GenBank database revealing that novel ammonia-oxidizing bacteria and nitrogen-fixing microorganisms may exist in this tropical marine environment. The observation of a large number of clones related to uncultured bacteria also indicates the necessity to describe these microorganisms and to develop new cultivation methodologies.

‣ Transfer RNA-dependent asparagine biosynthesis in Gluconacetobacter diazotrophicus and its influence on biological nitrogen fixation

Alquéres, Sylvia Maria Campbell; Cardoso, Alexander Machado; Silva, Jordano de Brito Moreira da; Baldani, José Ivo; Martins, Orlando Bonifácio
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Artigo de Revista Científica
Português
8 p. : il.; Background and aims Gluconacetobacter diazotrophicus is a nitrogen-fixing endophytic bacterium isolated from sugarcane, rice, elephant grass, sweet potato, coffee, and pineapple. These plants have high level of asparagine, which promotes microbial growth and inhibits nitrogenase activity. The regulation of intracellular concentrations of this amino acid is essential for growth and biological nitrogen fixation (BNF) in this diazotroph; however its asparagine metabolic pathway has not yet been clearly established. Methods The work reported here is the first to demonstrate the use of an alternative route for asparaginyl-tRNA (Asn-tRNA) and asparagine formation in an endophytic nitrogen-fixing bacterium by using in silico and in vitro analysis. Results The indirect route involves transamidation of incorrectly charged tRNA via GatCAB transamidase. Nitrogenase activity was completely inhibited by 20 mM Asn in LGI-P medium, which in contrast promotes protein synthesis and microbial growth. Conclusions The analysis carried out in this work shows that intracellular levels of asparagine regulate the expression of nitrogenase nifD gene (GDI0437), suggesting that the presence of an alternative route to produce asparagine might give the G. diazotrophicus a tighter control over cell growth and BNF...

‣ Influence of salinity on bacterioplankton communities from the brazilian rain forest to the coastal Atlantic Ocean

Silveira, Cynthia Barbosa da; Vieira, Ricardo Pilz; Cardoso, Alexander Machado; Paranhos, Rodolfo Pinheiro da Rocha; Albano, Rodolpho Mattos; Martins, Orlando Bonifácio
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Artigo de Revista Científica
Português
9 p. : il.; Background: Planktonic bacteria are recognized as important drivers of biogeochemical processes in all aquatic ecosystems, however, the taxa that make up these communities are poorly known. The aim of this study was to investigate bacterial communities in aquatic ecosystems at Ilha Grande, Rio de Janeiro, Brazil, a preserved insular environment of the Atlantic rain forest and how they correlate with a salinity gradient going from terrestrial aquatic habitats to the coastal Atlantic Ocean. Methodology/Principal Findings: We analyzed chemical and microbiological parameters of water samples and constructed 16S rRNA gene libraries of free living bacteria obtained at three marine (two coastal and one offshore) and three freshwater (water spring, river, and mangrove) environments. A total of 836 sequences were analyzed by MOTHUR, yielding 269 freshwater and 219 marine operational taxonomic units (OTUs) grouped at 97% stringency. Richness and diversity indexes indicated that freshwater environments were the most diverse, especially the water spring. The main bacterial group in freshwater environments was Betaproteobacteria (43.5%), whereas Cyanobacteria (30.5%), Alphaproteobacteria (25.5%), and Gammaproteobacteria (26.3%) dominated the marine ones. Venn diagram showed no overlap between marine and freshwater OTUs at 97% stringency. LIBSHUFF statistics and PCA analysis revealed marked differences between the freshwater and marine libraries suggesting the importance of salinity as a driver of community composition in this habitat. The phylogenetic analysis of marine and freshwater libraries showed that the differences in community composition are consistent. Conclusions/Significance: Our data supports the notion that a divergent evolutionary scenario is driving community composition in the studied habitats. This work also improves the comprehension of microbial community dynamics in tropical waters and how they are structured in relation to physicochemical parameters. Furthermore...

‣ Metagenomics in polluted aquatic environments

Cardoso, Alexander Machado; Coutinho, Felipe Hernandes
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Artigo de Revista Científica
Português
16 p. : il.; Metagenomics is defined as the culture-independent genomic analysis of biological assemblages providing access to the whole set of genes and genomes from a sample. It encompasses a variety of techniques that are based on (i) total DNA extraction from samples followed by PCR amplification of specific genes, (ii) library construction or amplification and sequencing of the whole genetic material. These methodologies have successfully been applied in studies of composition, dynamics, and functions of microbial communities in a variety of ecosystems including those subjected to anthropogenic modifications (Gilbert & Dupont, 2011). Culture independent methods allow the analysis of a set of metabolic genes from microbial communities, which can be used to determine how environmental conditions such as pollution can shape community composition and the diversity of genes associated with biogeochemical cycles such as those of carbon, nitrogen, and phosphorus (Singh et al., 2009). This approach is also useful for the discovery of novel environmental microorganisms and genes, with important applications for biotechnology, medicine, and bioremediation (Cardoso et al., 2011). This applicability has resulted in a recent sharp increase in studies focusing in the metagenomic analysis of polluted sites. Their aim is to characterize microbial communities from a diverse set of environments such as freshwater...

‣ Gut bacterial communities in the giant land snail achatina fulica and their modification by sugarcane-based diet

Cardoso, Alexander Machado; Cavalcante, Janaina Japiassu de Vasconcelos; Vieira, Ricardo Pilz; Lima, Joyce Lemos; Grieco, Maria Angela Bernardes; Clementino, Maysa Beatriz Mandetta; Vasconcelos, Ana Tereza Ribeiro de; Garcia, Eloi de Souza; Souza, Wanderl
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Artigo de Revista Científica
Português
6 p. : il.; The invasive land snail Achatina fulica is one of the most damaging agricultural pests worldwide representing a potentially serious threat to natural ecosystems and human health. This species is known to carry parasites and harbors a dense and metabolically active microbial community; however, little is known about its diversity and composition. Here, we assessed for the first time the complexity of bacterial communities occurring in the digestive tracts of field-collected snails (FC) by using culture-independent molecular analysis. Crop and intestinal bacteria in FC were then compared to those from groups of snails that were reared in the laboratory (RL) on a sugarcane-based diet. Most of the sequences recovered were novel and related to those reported for herbivorous gut. Changes in the relative abundance of Bacteroidetes and Firmicutes were observed when the snails were fed a high-sugar diet, suggesting that the snail gut microbiota can influence the energy balance equation. Furthermore, this study represents a first step in gaining a better understanding of land snail gut microbiota and shows that this is a complex holobiont system containing diverse, abundant and active microbial communities.

‣ Adaptor Protein-3 (AP-3) complex mediates the biogenesis of acidocalcisomes and is essential for growth and virulence of Trypanosoma brucei

Huang, Guozhong; Fang, Jianmin; Sant’Anna, Celso; Li, Zhu-Hong; Wellems, Dianne L.; Rohloff, Peter; Docampo, Roberto
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Artigo de Revista Científica
Português
12 p. : il.; Acidocalcisomes are acidic calcium and polyphosphate storage organelles found in a diverse range of organisms. Here we present evidence that the biogenesis of acidocalcisomes inTrypanosoma brucei is linked to the expression of adaptor protein-3 (AP-3) complex. Localization studies in cell lines expressing 3 and subunits of AP-3 fused to epitope tags revealed their partial co-localization with the vacuolar proton pyrophosphatase, a marker of acidocalcisomes, with the Golgi marker Golgi reassembly and stacking protein, and with antibodies against the small GTPase Rab11. Ablation of the 3 subunit by RNA interference (RNAi) resulted in disappearance of acidocalcisomes from both procyclic and bloodstream form trypanosomes, as revealed by immmunofluorescence and electron microscopy assays, with no alterations in trafficking of different markers to lysosomes. Knockdown of the 3 subunit resulted in lower acidic calcium, pyrophosphate, and polyphosphate content as well as defects in growth in culture, resistance to osmotic stress, and virulence in mice. Similar results were obtained by knocking down the expression of the subunit of AP-3. These results indicate that AP-3 is essential for the biogenesis of acidocalcisomes and for growth and virulence of T. brucei.

‣ The kinetoplast ultrastructural organization of endosymbiont-bearing trypanosomatids as revealed by deep-etching, cytochemical and immunocytochemical analysis

Cavalcanti, Danielle Pereira; Thiry, Marc; Souza, Wanderley de; Motta, Maria Cristina Machado
Fonte: Springer Publicador: Springer
Tipo: Artigo de Revista Científica
Português
9 p. : il.; The endosymbiont-bearing trypanosomatids present a typical kDNA arrangement, which is not well characterized. In the majority of trypanosomatids, the kinetoplast forms a bar-like structure containing tightly packed kDNA Wbers. On the contrary, in trypanosomatids that harbor an endosymbiotic bacterium, the kDNA Wbers are disposed in a looser arrangement that Wlls the kinetoplast matrix. In order to shed light on the kinetoplast structural organization in these protozoa, we used cytochemical and immunocytological approaches. Our results showed that in endosymbiont-containing species, DNA and basic proteins are distributed not only in the kDNA network, but also in the kinetoXagellar zone (KFZ), which corresponds to the region between the kDNA and the inner mitochondrial membrane nearest the Xagellum. The presence of DNA in the KFZ is in accordance with the actual model of kDNA replication, whereas the detection of basic proteins in this region may be related to the basic character of the intramitochondrial Wlaments found in this area, which are part of the complex that connects the kDNA to the basal body. The kinetoplast structural organization of Bodo sp. was also analyzed, since this protozoan lacks the highly ordered kDNA-packaging characteristic of trypanosomatid and represents an evolutionary ancestral of the Trypanosomatidae family.

‣ Knockout of the gene encoding the kinetoplast-associated protein 3 (KAP3) in Trypanosoma cruzi: effect on kinetoplast organization, cell proliferation and differentiation

Souza, Flavia Sá Pereira de; Rampazzo, Rita de Cássia Pontello; Manhaes, Lauro; Soares, Maurilio José; Cavalcanti, Danielle Pereira; Krieger, Marco Aurélio; Goldenberg, Samuel; Fragoso, Stênio Perdigão
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Artigo de Revista Científica
Português
9 p. : il.; Kinetoplast DNA (kDNA) of trypanosomatid protozoa consists of an unusual arrangement of two types of circular molecules catenated into a single network: (1) a few maxicircles that encode various mitochondrial enzyme subunits and rRNA in a cryptic pattern and (2) thousands of minicircles that encode guide RNAs (gRNAs). kDNA is associated with proteins, known as kinetoplast-associated proteins (KAPs), which condense the kDNA network. However, little is known about the KAPs of Trypanosoma cruzi, a parasite that displays different kDNA condensation patterns during its complex morphogenetic development. We cloned the T. cruzi gene encoding TcKAP3 (kinetoplast-associated protein 3). TcKAP3 is a single-copy gene that is transcribed into a 1.8-kb mRNA molecule and expressed in all stages of the parasite. Mouse antiserum raised against recombinant TcKPA3 recognized a 21.8 kDa protein, which was found, by indirect immunofluorescence and immunoelectron microscopy, to be associated with the T. cruzi kinetoplast. Several features of TcKAP3, such as its small size, basic nature and similarity with KAP3 from the insect trypanosomatid Crithidia fasciculata, are consistent with a role in DNA charge neutralization and condensation. This suggests that this protein is involved in organizing the kDNA network. Gene deletion was used to investigate TcKAP3 function. Here we investigated the T. cruzi KAP3 null mutant...

‣ Requisitos de avaliação da conformidade no desenvolvimento de produtos [slides]

Rocha, Leonardo Machado
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Trabalho apresentado em evento / Paper
Português
58 Slides : il.; No âmbito do Acordo de Cooperação Técnica Inmetro/SEBRAE, o Inmetro participou no XXI Seminário Nacional de Parques Tecnológicos e Incubadoras de Empresas, realizado no período de 24 a 28 de outubro de 2011, em Porto Alegre (RS), com a palestra sobre o tema Requisitos de Avaliação da Conformidade no desenvolvimento de produtos. Esse evento foi parte do projeto desenvolvido pela Associação Nacional de Entidades Promotoras de Empreendimetos Inovadores - ANPROTEC, em parceria com o SEBRAE e, dentre os vários objetivos propostos estava o de sensibilizar os empreendedores e os gestores de incubadoras sobre a importância estratégica da atividade de Avaliação da Conformidade. Neste contexto, a capacitação e a participação das empresas no processo de estabelecimento de Programas de Avaliação da Conformidade é fundamental para que as mesmas possam se antecipar aos impactos decorrentes da atividade.

‣ The Brasilian program of toy certification [slides]

Rocha, Leonardo Machado
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Trabalho apresentado em evento / Paper
Português
26 Slides : il.; Apresentação ministrada, em 18 de novembro de 2010, no Seminário sobre Regulamentação Italiana para Brinquedos e na Feira G! Come Giocare da indústria italiana de brinquedos, em Milão/Itália, visando debater e efetuar uma análise comparativa entre as práticas brasileira e européia na regulamentação de brinquedos.

‣ Medição de volume e de massa específica com um sistema de pesagem hidrostática de simples construção

Aibe, Valter Yoshihiko; Farias, Estela Cristina Cavalcante de
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Trabalho apresentado em evento / Paper
Português
5 p. : il.; Foi montado um sistema simples de pesagem hidrostática para as medições de volume de um corpo sólido e de massa específica de sólidos e líquidos. A água pura foi usada como material de referência de massa específica para a medição de volume de um poliedro construído em vidro. Com o volume do sólido calibrado, mediu-se a massa específica de diferentes líquidos. Embora seja um sistema fácil montagem, apresentou capacidade de resolução melhor que o densímetro digital de resolução 0,00001 g/cm3.

‣ Experimental analysis of a bubble plumein upward flow near a vertical cylinder

Farias, Maria Helena; Costa, Fabio Ouverney; Ramos, Marcos
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Trabalho apresentado em evento / Paper
Português
9 p. : il.; A complexity of multi-phase flow mixtures systems is encountered in many industrial applications. Due to the complexity, the hydrodynamic behavior of several types of multi-phase systems must be analyzed almost individually, because although there could be some similarity among a group of systems, a particular detail in kinematics of the flow makes each system different from the other. In special, for applications that involve controlled process of mixtures where plumes or jets are present, the features of the installation for gas or liquid injection points, as well as the geometry where the flow is set, play an important role in the quality of the expected final product. In order to contribute to the understanding of how this kind of mixture process could be optimized, the present work shows the flow characteristics of a single air bubble plume when a vertical surface is close to the upward plume in a stagnant Newtonian fluid. For that, a nozzle for air injection was placed at the bottom of a tank which contains the stagnant fluid. Two surfaces were separately placed near the plume: a flat and a cylindrical. The presence of the solid surface provokes the plume to cling in its direction. By using PIV technique for flow visualization...

‣ Avaliação da Conformidade e o Setor Elétrico [slides]

Kuster, Gustavo José
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Trabalho apresentado em evento / Paper
Português
34 Slides : il.; Palestra realizada na Asosec - Associação Colombiana de Organismos de Avaliação da Conformidade, em Junho de 2011, com o objetivo de apresentar o estado da Avaliação da Conformidade no setor elétrico brasileiro

‣ Registro [slides]

Kuster, Gustavo José
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Trabalho apresentado em evento / Paper
Português
21 Slides : il.; Palestra realizada em junho de 2011, na Associação Nacional dos Fabricantes de Pneus, sobre o processo de Registro no Inmetro, sua lógica e impactos na cadeia produtiva de pneus.

‣ Relatório : ouvidoria externa : 2011

Inmetro, Instituto Nacional de Metrologia, Qualidade e Tecnologia
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Relatório Técnico / Report
Português
31 f.: il.; Nesse Relatório apresentaremos os dados mais significativos, relativos ao relacionamento do Inmetro com o cidadão-usuário, no ano de 2011. Esses dados, sempre que possível, serão comparados ao ano anterior, permitindo avaliar tendências e realizar projeções. Mas, não poderia iniciar esse relatório sem, mais uma vez, justificar a demora em divulgar o mesmo. A razão do atraso permanece a mesma do ano anterior, isto é, dificuldades com as questões ligadas à tecnologia da informação. Nosso software SAC – Sistema de Atendimento ao cidadão, não nos fornecia dados que pudessem ser divulgados com confiança. Assim, parte do trabalho foi feito a moda antiga, manualmente. Retornando a análise dos dados coletados pela ouvidoria, em 2011, observamos uma diminuição das demandas registradas, em relação aos anos anteriores. Concluímos que esse decréscimo se devia a dois fatores importantes e muito impactantes, um positivo e outro negativo: o primeiro, positivo, foi a implantação, em nosso site, da busca pelo Google; o outro, negativo, foram os constantes problemas com a operadora de telefonia do 0800, que deixava a ouvidoria sem contato com a sociedade, por horas e as vezes, dias. Por fim, esclarecemos que este Relatório está divido em duas partes: a primeira com os dados gerais da Instituição; e a segunda parte com os dados das diretorias técnicas...

‣ Registro de objetos : objetivo : harmonizar entendimento sobre registro de objetos de produtos certificados [slides]

Kuster, Gustavo José
Fonte: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia Publicador: Inmetro - Instituto Nacional de Metrologia, Qualidade e Tecnologia
Tipo: Trabalho apresentado em evento / Paper
Português
14 slides : il.; Apresentação feita para os Organismos de Certificação de Produtos, no dia 25/04/12, auditório Inmetro na Sta. Alexandrina, no campo compulsório, sobre o processo de registro de objetos com a conformidade avaliada. Esse novo mecanismo muda, sensivelmente, a relação entre Inmetro e os Fornecedores, além de impactar também os Organismos de Certificação.