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‣ Evaluation of the expression of microRNAs in the response to therapy in Chronic Myeloid Leukemia = Avaliação da expressão de microRNAs na resposta à terapêutica na Leucemia Mieloide Crónica

Veiga, Sara Isabel Ferreira
Fonte: Universidade de Coimbra Publicador: Universidade de Coimbra
Tipo: Dissertação de Mestrado
Português
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A Leucemia Mieloide Crónica (LMC) é uma doença clonal mieloproliferativa caracterizada pela existência da translocação t(9:22), da qual resulta o cromossoma Filadélfia. Esta translocação dá origem ao gene de fusão BCR-ABL, que codifica a oncoproteina BCRABL com atividade de tirosina cinase aumentada. O Imatinib é um inibidor de tirosina cinase (TKI) que veio alterar a estratégia terapêutica habitualmente utilizada em doentes de LMC, o interferão alfa, e é atualmente o tratamento de primeira linha na doença. O Imatinib bloqueia especificamente a atividade da oncoproteina BCR-ABL e promove uma boa resposta por parte dos doentes. No entanto são conhecidos casos de resistência, sendo a razão mais comum o aparecimento de mutações no domínio BCR-ABL que impedem que o Imatinib se ligue com sucesso e exerça a sua função. Para além disto, existem outros mecanismos moleculares envolvidos na resistência a este fármaco, como a alteração da expressão dos transportadores de influxo e efluxo e das enzimas envolvidas na metabolização do fármaco, entre outros. Recentemente têm surgido várias terapias que atuam em vias de sinalização celular que se têm revelado cada vez com mais de interesse no tratamento da LMC. O Bortezomib...

‣ Caracterização de disintegrinas de venenos viperídeos como ferramentas seletivas na detecção ou inibição da função de integrinas; Characterization of viper venom disintegrins as tools for detecting and inhibiting integrin function

Wadsworth, Diego Alberto Butera
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 15/12/2003 Português
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As integrinas são proteínas de membrana envolvidas em diversos processos biológicos como embriogênese, inflamação e agregação plaquetária. A alteração de seu padrão de expressão está relacionada com processos patológicos como trombose e câncer, fazendo das integrinas ótimos indicadores da progressão destas doenças. Assim, a integrina α2β1 pode ser considerada como indicadora de angiogênese, sendo expressa nas células endoteliais durante o crescimento de novos vasos. Já a ausência da αIIbβ3 em plaquetas está relacionada com a doença de Glanzmann, caracterizada por uma agregação plaquetária deficiente, e sua presença em outros tipos celulares está relacionada com processos de metástase e invasão de tecidos. Estas integrinas contêm antagonistas naturais nos venenos de serpentes da família VIPERlDAE: As RGD-disintegrinas bloqueiam a integrina αIIbβ3 e as ECD-disintegrinas bloqueiam a integrina α2β1. Estas últimas formam parte de metaloproteinases com múltiplos domínios de aproximadamente 50 kDa. Interessados em desenvolver marcadores moleculares para estas integrinas, direcionamos nossos objetivos para: (1) a elucidação da região mínima das ECD-disintegrinas com atividade biológica e (2) construção de biomarcadores para a integrina α2β1 utilizando a região elucidada...

‣ Efeito antioxidante do óleo de pequi em cápsula nos pacientes com lúpus eritematoso sistêmico

Sousa, Thaís Muniz Montalvão
Fonte: Universidade de Brasília Publicador: Universidade de Brasília
Tipo: Dissertação
Português
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Dissertação (mestrado)—Universidade de Brasília, Faculdade de Medicina, Programa de Pós-Graduação em Ciências Médicas, 2012.; Introdução: Indicadores de estresse oxidativo, tais como produtos de oxidação de ácidos nucleicos, proteínas e lipídeos, estão aumentados em pacientes com lúpus eritematoso sistêmico (LES) em comparação com indivíduos saudáveis. Como o estresse oxidativo tem sido implicado na fisiopatogenia do LES e na aterosclerose precoce nesses pacientes, uma possibilidade é o uso adjuvante de antioxidantes, particularmente as formas de suplementação natural. Objetivos: Primários - verificar em pacientes com LES se a suplementação do óleo da polpa de pequi em cápsula reduz: 1) as lesões no DNA, mensuradas por meio do índice de dano ao DNA obtido com o ensaio do cometa; 2) o colesterol total e a fração lipoproteína de baixa densidade (LDL); 3) os níveis de proteína C reativa ultrassensível (PCRus); 4) os níveis de ácido úrico. Secundário – verificar, por meio de ensaio cometa, se o nível de dano ao DNA é aumentado nos pacientes com LES em relação ao apresentado por indivíduos controles saudáveis. Pacientes e Métodos: Foi realizado um ensaio clínico controlado randomizado...

‣ Use of a questionnaire in general practice to increase the recognition of patients with excessive alcohol consumption.

Wallace, P; Haines, A
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 29/06/1985 Português
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A self administered questionnaire, the health survey questionnaire, was designed to detect excessive alcohol consumption and mailed to patients who were registered with two general practices. Replies were received from 2572(75%) of 3452 patients. Excessive consumption was taken as 42 units of alcohol per week or more for men and 21 units per week or more for women: 1 unit = approximately 10 g ethanol and is equivalent to a half pint of beer, one glass of wine, or one standard measure of spirits. One hundred and twenty men (11%) and 68 women (5%) were identified by their responses to the questionnaire as excessive drinkers. According to their responses, roughly half of these expressed some concern about their drinking. In the practice where a disease register was kept 18 patients had been recorded as heavy drinkers before the study, and an additional 74 were detected by the questionnaire. Within 12 months after the questionnaire survey three groups of respondents were reviewed: (a) the excessive drinkers, (b) those who indicated concern about drinking but did not exceed the limits for excessive consumption, and (c) a random sample of those who were in neither of these two categories. Patients in these three groups were interviewed in a standard fashion to determine their alcohol consumption. Breath alcohol measurement was also carried out and a blood sample taken for the estimation of mean cell volume...

‣ A Novel Interaction Between HER2/neu and Cyclin E in Breast Cancer

Mittendorf, Elizabeth A.; Liu, Yanna; Tucker, Susan L.; McKenzie, Tamra; Qiao, Na; Akli, Said; Biernacka, Anna; Liu, Yan; Meijer, Laurent; Keyomarsi, Khandan; Hunt, Kelly K.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Português
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HER2/neu (HER2) and cyclin E are important prognostic indicators in breast cancer. Since both are involved in cell cycle regulation we investigated whether there was a direct interaction between the two. HER2 and cyclin E expression levels were determined in 395 breast cancer patients. Patients with HER2-overexpression and high levels of cyclin E had decreased 5-year disease-specific survival compared with low levels of cyclin E (14% versus 89%, P < .0001) In vitro studies were performed in which HER2-mediated activity in HER2-overexpressing breast cancer cell lines was downregulated by transfection with HER2 siRNA or treatment with trastuzumab. Cyclin E expression levels were determined, and functional effects investigated using kinase assays, MTT assays to assess cell viability as a marker of proliferation, and FACS analysis to determine cell cycle profiles. Decreased HER2-mediated signaling resulted in decreased expression of cyclin E, particularly the low molecular weight (LMW) isoforms. Decreased HER2 and LMW cyclin E expression had functional effects, including decreased cyclin E-associated kinase activity and decreased proliferation, due to increased apoptosis and an increased accumulation of cells in the G1 phase. In vivo studies performed in a HER2-overexpressing breast cancer xenograft model confirmed the effects of trastuzumab on cyclin E expression. Given the relationship between HER2 and cyclin E...

‣ Extra-Lysosomal Localization of Arylsulfatase B in Human Colonic Epithelium

Prabhu, Sanjiv V.; Bhattacharyya, Sumit; Guzman-Hartman, Grace; Macias, Virgilia; Kajdacsy-Balla, André; Tobacman, Joanne K.
Fonte: SAGE Publications Publicador: SAGE Publications
Tipo: Artigo de Revista Científica
Publicado em /03/2011 Português
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The enzyme arylsulfatase B (N-acetylgalactosamine-4-sulfatase; ARSB; ASB) removes 4-sulfate groups from the sulfated glycosaminoglycans (sGAG) chondroitin-4-sulfate (C4S) and dermatan sulfate (DS). Inborn deficiency of ARSB leads to the lysosomal storage disease mucopolysaccharidosis VI, characterized by accumulation of sGAG in vital organs, disruption of normal physiological processes, severe morbidity, and premature death. Recent published work demonstrated extra-lysosomal localization with nuclear and cell membrane ARSB observed in bronchial and colonic epithelial cells, cerebrovascular cells, and hepatic cells. In this report, the authors present ARSB immunostaining in a colonic microarray and show differences in distribution, intensity, and pattern of ARSB staining among normal colon, adenomas, and adenocarcinomas. Distinctive, intense luminal membrane staining was present in the normal epithelial cells but reduced in the malignancies and less in the grade 3 than in the grade 1 adenocarcinomas. In the normal cores, a distinctive pattern of intense cytoplasmic positivity at the luminal surface was followed by reduced staining deeper in the crypts. ARSB enzymatic activity was significantly greater in normal than in malignant tissue. These study findings affirm extra-lysosomal localization of ARSB and suggest that altered ARSB immunostaining and reduced activity may be useful indicators of malignant transformation in human colonic tissue.

‣ Dose Effect Evaluation and Therapeutic Window of the Neuro-EPO Nasal Application for the Treatment of the Focal Ischemia Model in the Mongolian Gerbil

Teste, Iliana Sosa; Tamos, Yuneidys Mengana; Cruz, Yamila Rodríguez; Cernada, Adriana Muñoz; Rodríguez, Janette Cruz; Martínez, Nelvis Subirós; Antich, Rosa Maria Coro; González-Quevedo, Alina; Rodríguez, Julio Cesar García
Fonte: The Scientific World Journal Publicador: The Scientific World Journal
Tipo: Artigo de Revista Científica
Publicado em 22/05/2012 Português
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Cerebrovascular disease is the third leading cause of death and the leading cause of disability in Cuba and in several developed countries. A possible neuroprotective agent is the rHu-EPO, whose effects have been demonstrated in models of brain ischemia. The Neuro-EPO is a derivative of the rHu-EPO that avoids the stimulation of erythropoiesis. The aim of this study was to determine the Neuro-EPO delivery into the central nervous system (CNS) to exert a neuroprotective effect in cerebral ischemia model of the Mongolian gerbil. The Neuro-EPO in a rate of 249.4 UI every 8 hours for 4 days showed 25% higher viability efficacy (P > 0.01), improving neurological score and behavior of the spontaneous exploratory activity, the preservation of CA3 areas of the hippocampus, the cortex, and thalamic nuclei in the focal ischemia model of the Mongolian gerbil. In summary, this study, the average dose-used Neuro-EPO (249.4 UI/10 μL/every 8 hours for 4 days), proved to be valid indicators of viability, neurological status, and spontaneous exploratory activity, being significantly lower than that reported for the systemically use of the rHu-EPO as a neuroprotectant. Indeed, up to 12 h after brain ischemia is very positive Neuro-EPO administration by the nasal route as a candidate for neuroprotection.

‣ Telomerase detection in the diagnosis and prognosis of cancer

Hiyama, Eiso; Hiyama, Keiko
Fonte: Kluwer Academic Publishers Publicador: Kluwer Academic Publishers
Tipo: Artigo de Revista Científica
Publicado em /06/2004 Português
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Telomerase, a critical enzyme responsible ‘for cellular immortality, is usually repressed in somatic cells except for lymphocytes and self-renewal cells, but is activated in approximately 85% of human cancer tissues. The human telomerase reverse transcriptase (hTERT) is the catalytic component of human telomerase. In cancers in which telomerase activation occurs at the early stages of the disease, telomerase activity and hTERT expression are useful markers for the detection of cancer cells. In other cancers in which telomerase becomes upregulated upon tumor progression, they are useful as prognostic indicators. However, careful attention should be paid to false–negative results caused by the instability of telomerase and of the hTERT mRNA and the presence of PCR inhibitors, as well as to false–positive results caused by the presence of alternatively spliced hTERT mRNA and normal cells with telomerase activity. Recently, methods for the in situ detection of the hTERT mRNA and protein have been developed. These methods should facilitate the unequivocal detection of cancer cells, even in tissues containing a background of normal telomerase-positive cells.

‣ Towards integrative physiological monitoring of the critically ill: from cardiovascular to microcirculatory and cellular function monitoring at the bedside

Donati, Abele; Tibboel, Dick; Ince, Can
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Português
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Current hemodynamic monitoring of critically ill patients is mainly focused on monitoring of pressure-derived hemodynamic variables related to systemic circulation. Increasingly, oxygen transport pathways and indicators of the presence of tissue dysoxia are now being considered. In addition to the microcirculatory parameters related to oxygen transport to the tissues, it is becoming increasingly clear that it is also important to gather information regarding the functional activity of cellular and even subcellular structures to gain an integrative evaluation of the severity of disease and the response to therapy. Crucial to these developments is the need to provide continuous measurements of the physiological and pathophysiological state of the patient, in contrast to the intermittent sampling of biomarkers. As technological research and clinical investigations into the monitoring of critically ill patients have progressed, an increasing amount of information is being made available to the clinician at the bedside. This complexity of information requires integration of the variables being monitored, which requires mathematical models based on physiology to reduce the complexity of the information and provide the clinician with a road map to guide therapy and assess the course of recovery. In this paper...

‣ Quantitative and qualitative differences in use and trends of hematopoietic stem cell transplantation: a Global Observational Study

Gratwohl, Alois; Baldomero, Helen; Gratwohl, Michael; Aljurf, Mahmoud; Bouzas, Luis Fernando; Horowitz, Mary; Kodera, Yoshihisa; Lipton, Jeff; Iida, Minako; Pasquini, Marcelo C.; Passweg, Jakob; Szer, Jeff; Madrigal, Alejandro; Frauendorfer, Karl; Niederw
Fonte: Ferrata Storti Foundation Publicador: Ferrata Storti Foundation
Tipo: Artigo de Revista Científica
Publicado em /08/2013 Português
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Fifty-five years after publication of the first hematopoietic stem cell transplantation this technique has become an accepted treatment option for defined hematologic and non-hematologic disorders. There is considerable interest in understanding differences in its use and trends on a global level and the macro-economic factors associated with these differences. Data on the numbers of hematopoietic stem cell transplants performed in the 3-year period 2006–2008 were obtained from Worldwide Network for Blood and Marrow Transplantation member registries and from transplant centers in countries without registries. Population and macro-economic data were collected from the World Bank and from the International Monetary Fund. Transplant rates were analyzed by indication, donor type, country, and World Health Organization regional offices areas and related to selected health care indicators using single and multiple linear regression analyses. Data from a total of 146,808 patients were reported by 1,411 teams from 72 countries over five continents. The annual number of transplants increased worldwide with the highest relative increase in the Asia Pacific region. Transplant rates increased preferentially in high income countries (P=0.02)...

‣ Plasma Selenium Biomarkers in Low Income Black and White Americans from the Southeastern United States

Hargreaves, Margaret K.; Liu, Jianguo; Buchowski, Maciej S.; Patel, Kushal A.; Larson, Celia O.; Schlundt, David G.; Kenerson, Donna M.; Hill, Kristina E.; Burk, Raymond F.; Blot, William J.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 20/01/2014 Português
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Biomarkers of selenium are necessary for assessing selenium status in humans, since soil variation hinders estimation of selenium intake from foods. In this study, we measured the concentration of plasma selenium, selenoprotein P (SEPP1), and glutathione peroxidase (GPX3) activity and their interindividual differences in 383 low-income blacks and whites selected from a stratified random sample of adults aged 40–79 years, who were participating in a long-term cohort study in the southeastern United States (US). We assessed the utility of these biomarkers to determine differences in selenium status and their association with demographic, socio-economic, dietary, and other indicators. Dietary selenium intake was assessed using a validated food frequency questionnaire designed for the cohort, matched with region-specific food selenium content, and compared with the US Recommended Dietary Allowances (RDA) set at 55 µg/day. We found that SEPP1, a sensitive biomarker of selenium nutritional status, was significantly lower among blacks than whites (mean 4.4±1.1 vs. 4.7±1.0 mg/L, p = 0.006), with blacks less than half as likely to have highest vs. lowest quartile SEPP1 concentration (Odds Ratio (OR) 0.4, 95% Confidence Interval (CI) 0.2–0.8). The trend in a similar direction was observed for plasma selenium among blacks and whites...

‣ Estudo clínico-laboratorial e histopatológico de cães naturalmente infectados por Leishmania chagasi com diferentes graus de manifestação física

Coutinho, José Flávio Vidal
Fonte: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Bioquímica; Bioquímica; Biologia Molecular Publicador: Universidade Federal do Rio Grande do Norte; BR; UFRN; Programa de Pós-Graduação em Bioquímica; Bioquímica; Biologia Molecular
Tipo: Dissertação Formato: application/pdf
Português
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Canine Visceral Leishmania (CVL) is an important zoonotic disease that has a world wide distribution and has a large impact on public health on the American Continent, especially in Brazil, where the nature of endemic diseases in humans affects a large part of the nation. The influence of the prevalence of CVL in the increased rate of human cases in endemic areas and in the unleashing of epidemic outbreaks shows the need for a more profound understanding, that would generate significant advances in the current measures used to control the reservoirs of sickness that are practiced by the Programa Nacional de Vigilância e Controle da Leishmaniose Visceral. The present work describes and compares the clinical-laboratorial and histopathological findings of twenty-three dogs that were naturally infected by Leishmania chagasi, from endemic areas in metropolitan Natal, Rio Grande do Norte, Brazil. These animals, that were selected and given physical and serological exams (IFI and ELISA rK-39), were classified according to the degree of clinical severity and had blood samples drawn (whole blood and serum) for a complete hemogram and a coagulogram to be done as well as biochemical tests for kidney and liver function. The confirmation of infection by L. chagasi was done after the euthanasia of the animals...

‣ Romania - Functional Review : Health Sector

World Bank
Fonte: Washington, DC Publicador: Washington, DC
Português
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This Functional Review was carried by a Bank team upon request by the Government of Romania. As a starting point, it shows that: (i) health outcomes in Romania lag behind those of the EU; (ii) users are not satisfied with its lack of responsiveness - long lines, informal payments, discourteous handling of patients, poor cleanliness, lack of maintenance, and breach of safety measures; (iii) the poor and other vulnerable groups (for example Roma communities) suffer from a significant lack of access to services; and (iv) the fiscal contraction of 2008-2010 exposed the weakness of financial controls in the health sector. The Review examines four health functions in depth: service delivery, financing, stewardship and resources (including pharmaceuticals). Based on our findings, we present recommendations categorized by the three set of challenges that the sector is facing: (i) improving governance and management; (ii) streamlining the health service network and re-launching quality control systems; and (iii) increasing preventive services and equity. For any expansion of the sector to be sustainable...

‣ Situational Analysis Improving Economic Outcomes by Expanding Nutrition Programming in Tajikistan

World Bank; UNICEF
Fonte: World Bank, Washington, DC Publicador: World Bank, Washington, DC
Português
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Undernutrition in Tajikistan remains an important public health challenge, albeit a hidden problem. Stunting, iodine deficiency, and maternal and child anemia represent the largest burden of undernutrition in Tajikistan. In 2009, around 29 percent of children in all regions of the country were stunted. Iodine deficiency was observed in 53 percent of children and in 58.6 percent of women. The national prevalence of anemia in children was 28.8 percent; however, rates were as high as 39.8 percent in Ghorno-Badakhshan Autonomous Province and in 32 percent directly ruled districts (DRD). The prevalence of anemia among mothers was 24.2 percent. The long-term effects of these conditions negatively affect the health of adults throughout their life, as well as their potential productivity in the work force and possible economic contribution to the nation. The highest priority interventions will improve infant and young child feeding. Strengthening and scaling up breastfeeding promotion will save lives and help to reduce stunting as would complementary feeding for babies six months and older. Promoting exclusive breastfeeding for infants under six months is the most efficacious intervention to save lives...

‣ Public Health in the Middle East and North Africa : Meeting the Challenges of the Twenty-first Century

Pierre-Louis, Anne Maryse; Akala, Francisca Ayodeji; Karam, Hadia Samaha
Fonte: Washington, DC: World Bank Publicador: Washington, DC: World Bank
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This report documents the main highlights and findings from the Middle East Regional Symposium, "Meeting the Public Health Challenges of the 21st Century in the Middle East and North Africa/Eastern Mediterranean Region", held in Beirut in June 2002. The Middle East and North Africa (MENA) region is currently undergoing both epidemiological and demographical transitions which are resulting in changing patterns of mortality and morbidity. Chronic and non-communicable diseases and injuries now account for a growing share of the overall burden of ill health. Countries of the region are faced with the challenge of addressing these emerging issues while, at the same time trying to complete the unfinished agenda of addressing diseases and protecting the health of mothers and children. The book consists of an overview drawing on a broad range of presentations at the conference, followed by chapters on specific public health issues including: functions and infrastructures, choosing and financing public health services...

‣ Management of asthma in Australian general practice: Care is still not in line with clinical practice guidelines

Barton, C.; Proudfoot, J.; Amoroso, C.; Ramsay, E.; Holton, C.; Bubner, T.; Harris, M.; Beilby, J.
Fonte: Strategic Medical Publishing Publicador: Strategic Medical Publishing
Tipo: Artigo de Revista Científica
Publicado em //2009 Português
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OBJECTIVE AND BACKGROUND: We investigated the quality of primary care asthma management in a sample of Australian general practices. METHODS: 247 general practitioners (GPs) from 97 practices completed a structured interview about management of asthma, diabetes and hypertension/heart disease. A further structured interview with the senior practice principal and practice manager was used to collect information about practice capacity for chronic disease management. RESULTS: Just under half of GPs (47%) had access to an asthma register and the majority (76%) had access to spirometry in their practice. In terms of routine management of asthma, 12% of GPs reported using spirometry routinely, 13% routinely reviewed written asthma action plans, 27% routinely provided education about trigger factors, 30% routinely reviewed inhaler technique, 24% routinely assessed asthma severity, and 29% routinely assessed physical activity. Practice characteristics such as practice size (p=1.0) and locality (rural/metropolitan) (p=0.7) did not predict quality of asthma management nor did indicators of practice capacity including Business maturity, IT/IM maturity, Multidisciplinary teamwork, and Clinical linkages. CONCLUSION: Gaps remain in the provision of evidence-based care for patients with asthma in general practice. Markers of practice capacity measured here were not associated with guideline-based respiratory care within practices.; Christopher Barton...

‣ The Clinical Translation Gap in Child Health Exercise Research: A Call for Disruptive Innovation

Ashish, Naveen; Bamman, Marcas; Cerny, Frank; Cooper, Dan; d'Memecourt, Pierre; Eisenmann, Joey; Ericson, Dawn; Fahey, John; Falk, Baraket; Gabriel, Davera; Kahn, Michael; Kemper, Han; Leu, Szu-Yun; McMurray, Robert; Nixon, Patricia; Olin, J; Pianosi, Pao
Fonte: Wiley Publicador: Wiley
Tipo: Artigo de Revista Científica
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In children, levels of play, physical activity, and fitness are key indicators of health and disease and closely tied to optimal growth and development. Cardiopulmonary exercise testing (CPET) provides clinicians with biomarkers of disease and effectiveness of therapy, and researchers with novel insights into fundamental biological mechanisms reflecting an integrated physiological response that is hidden when the child is at rest. Yet the growth of clinical trials utilizing CPET in pediatrics remains stunted despite the current emphasis on preventative medicine and the growing recognition that therapies used in children should be clinically tested in children. There exists a translational gap between basic discovery and clinical application in this essential component of child health. To address this gap, the NIH provided funding through the Clinical and Translational Science Award (CTSA) program to convene a panel of experts. This report summarizes our major findings and outlines next steps necessary to enhance child health exercise medicine translational research. We present specific plans to bolster data interoperability, improve child health CPET reference values, stimulate formal training in exercise medicine for child health care professionals...

‣ p53 Regulates the Formation of Lamellipodia and Circular Dorsal Ruffles Through Caldesmon and PTEN

VANDENBERG, Laura Joanna
Fonte: Quens University Publicador: Quens University
Tipo: Tese de Doutorado
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Vascular smooth muscle cell migration is a significant contributor to many aspects of heart disease, and specifically atherosclerosis. Tissue damage in the arteries can result in the formation of a fatty streak. Smooth muscle cells (SMC) can then migrate to this site to form a fibrous cap, stabilizing the fatty plaque. Since cardiovascular disease is the leading cause of death in developed countries, this function of SMC is an essential area of study. The formation of lamellipodia and circular dorsal ruffles were studied in this project as indicators that cell migration is occurring. The roles of the proteins p53, Rac, caldesmon and PTEN were investigated with regards to these actin-based structures. The tumour suppressor p53 is often reported to cause apoptosis, senescence or cell cycle arrest when stress is placed on a cell, but has recently been shown to regulate cell migration as well. It was determined in this project that p53 could inhibit the formation of both lamellipodia and circular dorsal ruffles. It was also shown that this could occur directly through an inhibition of the GTPase Rac. Previous studies have shown that p53 can upregulate caldesmon, a protein which is known to bind to and stabilize actin filaments while inhibiting Arp2/3-mediated branching. It was confirmed that p53 could upregulate caldesmon...

‣ Efeitos da vibração na qualidade da uva 'Niagara Rosada'; The effects of Vibration in the Quality of 'Niagara Rosada' Grape

Daniel Gomes
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 22/12/2006 Português
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A viticultura nacional encontra-se em pleno crescimento e desenvolvimento. É atividade de importância social necessitando de intensa mão-de-obra especializada e seu produto apresenta ótimo valor agregado. Dos dois tipos de uvas de mesa existentes, as 'Finas' e as 'Comuns', a 'Niagara Rosada' pertence ao último, sendo a uva de maior consumo no Brasil e considerada como referência de uva de mesa brasileira. É fruta sensível e pode sofrer danos na colheita, beneficiamento e transporte. Este trabalho teve como objetivo principal avaliar o efeito da vibração na qualidade da uva 'Niagara Rosada', das classes III e V, acondicionada em embalagens de madeira e papelão ondulado. As uvas foram submetidas a um período de vibração simulando transporte rodoviário; foram armazenadas e observadas segundo a variação temporal da qualidade avaliada, dando-se notas aos quesitos tais como dano profundo, doença, degrana, dano leve, aparência do engaço, perda de massa, firmeza do bago e força de destacamento do pedúnculo, num período de cinco dias de armazenamento. Durante as avaliações foram encontrados dois tipos de danos, classificados como atemporais, que podem ser caracterizados logo após a solicitação mecânica e representados pelo dano leve e dano profundo e os danos classificados como temporais que...

‣ Global Estimates of the Burden of Injury and Illness at Work in 2012

Takala, Jukka; Hämäläinen, Päivi; Saarela, Kaija Leena; Yun, Loke Yoke; Manickam, Kathiresan; Jin, Tan Wee; Heng, Peggy; Tjong, Caleb; Kheng, Lim Guan; Lim, Samuel; Lin, Gan Siok
Fonte: Taylor & Francis Publicador: Taylor & Francis
Tipo: Artigo de Revista Científica
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This article reviews the present indicators, trends, and recent solutions and strategies to tackle major global and country problems in safety and health at work. The article is based on the Yant Award Lecture of the American Industrial Hygiene Association (AIHA) at its 2013 Congress. We reviewed employment figures, mortality rates, occupational burden of disease and injuries, reported accidents, surveys on self-reported occupational illnesses and injuries, attributable fractions, national economic cost estimates of work-related injuries and ill health, and the most recent information on the problems from published papers, documents, and electronic data sources of international and regional organizations, in particular the International Labor Organization (ILO), World Health Organization (WHO), and European Union (EU), institutions, agencies, and public websites. We identified and analyzed successful solutions, programs, and strategies to reduce the work-related negative outcomes at various levels. Work-related illnesses that have a long latency period and are linked to ageing are clearly on the increase, while the number of occupational injuries has gone down in industrialized countries thanks to both better prevention and structural changes. We have estimated that globally there are 2.3 million deaths annually for reasons attributed to work. The biggest component is linked to work-related diseases...