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‣ Avaliação clínica de inlays e onlays confeccionados com dois tipos de cerâmica após 02 anos. ; Clinical evaluation of ceramic inlays and onlays made with two systems after 2 years

Santos, Maria Jacinta Moraes Coelho
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 10/03/2003 Português
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As restaurações em cerâmica pura têm sido uma opção restauradora largamente utilizada em dentes posteriores devido a sua excelente estética. Diversos tipos de sistemas cerâmicos estão disponíveis no mercado para a fabricação de restaurações parciais e coroas. Este trabalho teve o objetivo de avaliar o comportamento clínico de restaurações, do tipo inlay e onlay, confeccionadas com dois sistemas cerâmicos: cerâmica convencional (Duceram, Dentsply-Degussa) – D e cerâmica prensada (IPS Empress, Ivoclar- Vivadent) – IPS pelo período de 02 anos. Oitenta e seis restaurações, sendo 44 IPS e 42 D, foram cimentadas em 35 pacientes de ambos os sexos, com idade média de 35 anos. Vinte e sete pré-molares e cinqüenta e nove molares receberam preparos cavitários classe II, num total de 33 onlays e 53 inlays. Todas as restaurações foram fixadas com cimento resinoso dual (Variolink II, Ivoclar-Vivadent) e sistema adesivo Syntac (Primer e Adhesive) e Heliobond (Ivoclar-Vivadent), sob isolamento absoluto. Os procedimentos operatórios foram realizados por apenas um operador. As avaliações foram realizadas por dois examinadores independentes no baseline, após 01 e 02 anos mediante o critério USPHS modificado, quanto aos aspectos: sensibilidade pulpar...

‣ Sítio PT-02-Sotéia: análise dos processos formativos de um cerrito na região sudoeste da Laguna dos Patos/RS; Site PT-02-Sotéia: analysis of the formation process of a Cerrito in the a region southwestern of the Laguna dos Patos/RS

Loureiro, Andre Garcia
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 13/03/2008 Português
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Este trabalho consiste em um estudo dos processos de formação do Sítio PT-02-Sotéia. Este cerrito se localiza na região sudoeste da Laguna dos Patos, Rio Grande do Sul, Brasil. O trabalho procura compreender a estrutura do sítio através da análise distribucional da cultura material e das estruturas arqueológicas. Tem-se, também como objetivo o estudo da cultura material com o intuito de caracterizar a funcionalidade do sítio. No final se proporá uma análise comparativa com outros estudos de caso - em uma esfera de estudo intra-sítio - na região do Prata, com o fito de perceber diferenças e semelhanças na estrutura interna do PT-O2 em uma escala mais ampla de análise. ; This work consists to the study of the formation process of site PT-02-Sotéia. This mound (Cerrito) is located in the region southwestern of Laguna dos Patos, state of Rio Grande do Sul/Brazil. This work search perceive the structure of the site through analysis of the spatial distribution of the material culture and the archeological structures. Other objective of this research on the analysis of the material culture for characterizes of the functionality this site. Comparison with others works of the analysis inter-site about Cerritos in the region of the Rio da Prata (South America)...

‣ Tapasin Discriminates Peptide-Human Leukocyte Antigen-A*02:01 Complexes Formed with Natural Ligands*

Roder, Gustav; Geironson, Linda; Rasmussen, Michael; Harndahl, Mikkel; Buus, Søren; Paulsson, Kajsa
Fonte: American Society for Biochemistry and Molecular Biology Publicador: American Society for Biochemistry and Molecular Biology
Tipo: Artigo de Revista Científica
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A plethora of peptides are generated intracellularly, and most peptide-human leukocyte antigen (HLA)-I interactions are of a transient, unproductive nature. Without a quality control mechanism, the HLA-I system would be stressed by futile attempts to present peptides not sufficient for the stable peptide-HLA-I complex formation required for long term presentation. Tapasin is thought to be central to this essential quality control, but the underlying mechanisms remain unknown. Here, we report that the N-terminal region of tapasin, Tpn1–87, assisted folding of peptide-HLA-A*02:01 complexes according to the identity of the peptide. The facilitation was also specific for the identity of the HLA-I heavy chain, where it correlated to established tapasin dependence hierarchies. Two large sets of HLA-A*02:01 binding peptides, one extracted from natural HLA-I ligands from the SYFPEITHI database and one consisting of medium to high affinity non-SYFPEITHI ligands, were studied in the context of HLA-A*02:01 binding and stability. We show that the SYFPEITHI peptides induced more stable HLA-A*02:01 molecules than the other ligands, although affinities were similar. Remarkably, Tpn1–87 could functionally discriminate the selected SYFPEITHI peptides from the other peptide binders with high sensitivity and specificity. We suggest that this HLA-I- and peptide-specific function...

‣ HLA-A*02:07 Is a Protective Allele for EBV Negative and a Susceptibility Allele for EBV Positive Classical Hodgkin Lymphoma in China

Huang, Xin; Hepkema, Bouke; Nolte, Ilja; Kushekhar, Kushi; Jongsma, Theo; Veenstra, Rianne; Poppema, Sibrand; Gao, Zifen; Visser, Lydia; Diepstra, Arjan; van den Berg, Anke
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 15/02/2012 Português
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HLA-A2 protects from EBV+ classical Hodgkin lymphoma (cHL) in Western Europe, but it is unknown whether this protective effect also exists in the Chinese population. We investigated the association of HLA-A2 and specific common and well documented HLA-A2 subtypes with EBV stratified cHL patients (n = 161) from the northern part of China. Quantitative-PCR and sequence-based subtyping was performed to identify HLA-A2 positive samples and their subtypes. 67 (42%) of the cHL patients were EBV+. There were no significant differences in percentages of HLA-A2 positivity between cHL and controls (65% vs 66%) and between EBV+ and EBV− cHL patients (70% vs 61%). The frequency distribution of HLA-A2 subtypes was significantly different between EBV stratified cHL subgroups and controls. This difference was most striking for the HLA-A*02:07 type with a frequency of 38% in EBV+ cHL, 8% in EBV− cHL and 20% in controls. Significant differences were also observed for the HLA-A*02:07, HLA-A2 (non-02:07) and the A2-negative typings between EBV+ cHL vs controls (p = 0.028), EBV− cHL vs controls (p = 0.045) and EBV+ vs EBV− cHL cases (p = 2×10−5). In conclusion, HLA-A*02:07 is a predisposing allele for EBV+ cHL and a protective allele for EBV− cHL in the northern Chinese population.

‣ Multinucleation and cell dysfunction induced by amorphous silica nanoparticles in an L-02 human hepatic cell line

Wang, Wen; Li, Yang; Liu, Xiaomei; Jin, Minghua; Du, Haiying; Liu, Ying; Huang, Peili; Zhou, Xianqing; Yuan, Lan; Sun, Zhiwei
Fonte: Dove Medical Press Publicador: Dove Medical Press
Tipo: Artigo de Revista Científica
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Silica nanoparticles (SNPs) are one of the most important nanomaterials, and have been widely used in a variety of fields. Therefore, their effects on human health and the environment have been addressed in a number of studies. In this work, the effects of amorphous SNPs were investigated with regard to multinucleation in L-02 human hepatic cells. Our results show that L-02 cells had an abnormally high incidence of multinucleation upon exposure to silica, that increased in a dose-dependent manner. Propidium iodide staining showed that multinucleated cells were arrested in G2/M phase of the cell cycle. Increased multinucleation in L-02 cells was associated with increased generation of cellular reactive oxygen species and mitochondrial damage on flow cytometry and confocal microscopy, which might have led to failure of cytokinesis in these cells. Further, SNPs inhibited cell growth and induced apoptosis in exposed cells. Taken together, our findings demonstrate that multinucleation in L-02 human hepatic cells might be a failure to undergo cytokinesis or cell fusion in response to SNPs, and the increase in cellular reactive oxygen species could be responsible for the apoptosis seen in both mononuclear cells and multinucleated cells.

‣ DB-02, a C-6-Cyclohexylmethyl Substituted Pyrimidinone HIV-1 Reverse Transcriptase Inhibitor with Nanomolar Activity, Displays an Improved Sensitivity against K103N or Y181C Than S-DABOs

Zhang, Xing-Jie; Lu, Li-He; Wang, Rui-Rui; Wang, Yue-Ping; Luo, Rong-Hua; Cong Lai, Christopher; Yang, Liu-Meng; He, Yan-Ping; Zheng, Yong-Tang
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 25/11/2013 Português
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6-(cyclohexylmethyl)-5-ethyl-2-((2-oxo-2-phenylethyl)thio)pyrimidin-4(3H)-one (DB-02) is a member of the newly reported synthetic anti-HIV-1 compounds dihydro-aryl/alkylsulfanyl-cyclohexylmethyl-oxopyrimidines, S-DACOs. In vitro anti-HIV-1 activity and resistance profile studies have suggested that DB-02 has very low cytotoxicity (CC50>1mM) to cell lines and peripheral blood mononuclear cells (PBMCs). It displays potent anti-HIV-1 activity against laboratory adapted strains and primary isolated strains including different subtypes and tropism strains (EC50s range from 2.40 to 41.8 nM). Studies on site-directed mutagenesis, genotypic resistance profiles revealed that V106A was the major resistance contributor for the compound. Molecular docking analysis showed that DB-02 located in the hydrophobic pocket with interactions of Lys101, Val106, Leu234, His235. DB-02 also showed non-antagonistic effects to four approved antiretroviral drugs. All studies indicated that DB-02 would be a potential NNRTI with low cytotoxicity and improved activity.

‣ Human Leukocyte Antigens and Systemic Lupus Erythematosus: A Protective Role for the HLA-DR6 Alleles DRB1*13:02 and *14:03

Furukawa, Hiroshi; Kawasaki, Aya; Oka, Shomi; Ito, Ikue; Shimada, Kota; Sugii, Shoji; Hashimoto, Atsushi; Komiya, Akiko; Fukui, Naoshi; Kondo, Yuya; Ito, Satoshi; Hayashi, Taichi; Matsumoto, Isao; Kusaoi, Makio; Amano, Hirofumi; Nagai, Tatsuo; Hirohata, S
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 03/02/2014 Português
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Many studies on associations between human leukocyte antigen (HLA) allele frequencies and susceptibility to systemic lupus erythematosus (SLE) have been performed. However, few protective associations with HLA-DRB1 alleles have been reported. Here, we sought protective, as well as predispositional, alleles of HLA-DRB1 in Japanese SLE patients. An association study was conducted for HLA-DRB1 in Japanese SLE patients. Relative predispositional effects were analyzed by sequential elimination of carriers of each allele with the strongest association. We also explored the association of DRB1 alleles with SLE phenotypes including the presence of autoantibody and clinical manifestations. Significantly different carrier frequencies of certain DRB1 alleles were found to be associated with SLE as follows: increased DRB1*15:01 (P = 5.48×10−10, corrected P (Pc) = 1.59×10−8, odds ratio [OR] 2.17, 95% confidence interval [CI] 1.69–2.79), decreased DRB1*13:02 (P = 7.17×10−5, Pc = 0.0020, OR 0.46, 95% CI 0.34–0.63) and decreased DRB1*14:03 (P = 0.0010, Pc = 0.0272, OR 0.34, 95% CI 0.18–0.63). Additionally, the “*15:01/*13:02 or *14:03” genotype tended to be negatively associated with SLE (P = 0.4209, OR 0.66)...

‣ Full screening and accurate subtyping of HLA-A*02 alleles through group-specific amplification and mono-allelic sequencing

Song, Shengli; Han, Miaomiao; Zhang, Han; Wang, Yuanxia; Jiang, Hong
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
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HLA-A*02 is the most prevalent and polymorphic major histocompatibility complex (MHC) allele family in humans. Functional differences have been revealed among subtypes, demanding further subtyping of HLA-A*02 in basic and clinical settings. However, the fast growing polymorphisms render traditional primer- or probe-based typing methods impractical and result in increasing ambiguities in direct sequence-based typing. In this study, we combined group-specific amplification and mono-allelic sequencing to design and validate a simple scheme for the complete screening and accurate subtyping of all 540 reported HLA-A*02 alleles. This scheme could be performed in routine labs to facilitate studies with an interest in HLA-A*02.

‣ HLA-A*01:03, HLA-A*24:02, HLA-B*08:01, HLA-B*27:05, HLA-B*35:01, HLA-B*44:02, and HLA-C*07:01 Monochain Transgenic/H-2 Class I Null Mice: Novel Versatile Preclinical Models of Human T Cell Responses

Boucherma, Rachid; Kridane-Miledi, Hédia; Bouziat, Romain; Rasmussen, Michael; Gatard, Tanja; Langa-Vives, Francina; Lemercier, Brigitte; Lim, Annick; Bérard, Marion; BenMohamed, Lbachir; Buus, Søren; Rooke, Ronald; Lemonnier, François A.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
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We have generated a panel of transgenic mice expressing HLA-A*01:03, -A*24:02, -B*08:01, -B*27:05, -B*35:01, -B*44:02, or -C*07:01 as chimeric monochain molecules (i.e., appropriate HLA α1α2 H chain domains fused with a mouse α3 domain and covalently linked to human β2-microglobulin). Whereas surface expression of several transgenes was markedly reduced in recipient mice that coexpressed endogenous H-2 class I molecules, substantial surface expression of all human transgenes was observed in mice lacking H-2 class I molecules. In these HLA monochain transgenic/H-2 class I null mice, we observed a quantitative and qualitative restoration of the peripheral CD8+ T cell repertoire, which exhibited a TCR diversity comparable with C57BL/6 WT mice. Potent epitope-specific, HLA-restricted, IFN-γ–producing CD8+ T cell responses were generated against known reference T cell epitopes after either peptide or DNA immunization. HLA-wise, these new transgenic strains encompass a large proportion of individuals from all major human races and ethnicities. In combination with the previously created HLA-A*02:01 and -B*07:02 transgenic mice, the novel HLA transgenic mice described in this report should be a versatile preclinical animal model that will speed up the identification and optimization of HLA-restricted CD8+ T cell epitopes of potential interest in various autoimmune human diseases and in preclinical evaluation of T cell–based vaccines.

‣ Asymptomatic HLA-A*02:01–Restricted Epitopes from Herpes Simplex Virus Glycoprotein B Preferentially Recall Polyfunctional CD8+ T Cells from Seropositive Asymptomatic Individuals and Protect HLA Transgenic Mice against Ocular Herpes

Dervillez, Xavier; Qureshi, Huma; Chentoufi, Aziz A.; Khan, Arif A.; Kritzer, Elizabeth; Yu, David C.; Diaz, Oscar R.; Gottimukkala, Chetan; Kalantari, Mina; Villacres, Maria C.; Scarfone, Vanessa M.; McKinney, Denise M.; Sidney, John; Sette, Alessandro;
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
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Evidence from C57BL/6 mice suggests that CD8+ T cells, specific to the immunodominant HSV-1 glycoprotein B (gB) H-2b–restricted epitope (gB498–505), protect against ocular herpes infection and disease. However, the possible role of CD8+ T cells, specific to HLA-restricted gB epitopes, in protective immunity seen in HSV-1–seropositive asymptomatic (ASYMP) healthy individuals (who have never had clinical herpes) remains to be determined. In this study, we used multiple prediction algorithms to identify 10 potential HLA-A*02:01–restricted CD8+ T cell epitopes from the HSV-1 gB amino acid sequence. Six of these epitopes exhibited high-affinity binding to HLA-A*02:01 molecules. In 10 sequentially studied HLA-A*02:01–positive, HSV-1–seropositive ASYMP individuals, the most frequent, robust, and polyfunctional CD8+ T cell responses, as assessed by a combination of tetramer, IFN-γ-ELISPOT, CFSE proliferation, CD107a/b cytotoxic degranulation, and multiplex cytokine assays, were directed mainly against epitopes gB342–350 and gB561–569. In contrast, in 10 HLA-A*02:01–positive, HSV-1–seropositive symptomatic (SYMP) individuals (with a history of numerous episodes of recurrent clinical herpes disease) frequent, but less robust...

‣ LPSF/GQ-02 Inhibits the Development of Hepatic Steatosis and Inflammation in a Mouse Model of Non-Alcoholic Fatty Liver Disease (NAFLD)

Soares e Silva, Amanda Karolina; de Oliveira Cipriano Torres, Dilênia; dos Santos Gomes, Fabiana Oliveira; dos Santos Silva, Bruna; Lima Ribeiro, Edlene; Costa Oliveira, Amanda; dos Santos, Laise Aline Martins; de Lima, Maria do Carmo Alves; Pitta, Ivan
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 14/04/2015 Português
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Non-alcoholic fatty liver disease (NAFLD) defines a wide spectrum of liver diseases that extends from simple steatosis to non-alcoholic steatohepatitis. Although the pathogenesis of NAFLD remains undefined, it is recognized that insulin resistance is present in almost all patients who develop this disease. Thiazolidinediones (TZDs) act as an insulin sensitizer and have been used in the treatment of patients with type 2 diabetes and other insulin-resistant conditions, including NAFLD. Hence, therapy of NAFLD with insulin-sensitizing drugs should ideally improve the key hepatic histological changes, while also reducing cardiometabolic and cancer risks. Controversially, TZDs are associated with the development of cardiovascular events and liver problems. Therefore, there is a need for the development of new therapeutic strategies to improve liver function in patients with chronic liver diseases. The aim of the present study was to assess the therapeutic effects of LPSF/GQ-02 on the liver of LDLR-/- mice after a high-fat diet. Eighty male mice were divided into 4 groups and two different experiments: 1-received a standard diet; 2-fed with a high-fat diet (HFD); 3–HFD+pioglitazone; 4–HFD+LPSF/GQ-02. The experiments were conducted for 10 or 12 weeks and in the last two or four weeks respectively...

‣ 7.02 Introduction to Experimental Biology, Fall 2001; Introduction to Experimental Biology

Amon, Angelika; Rich, Alexander; Pardue, Mary Lou; Chess, Andrew; Schneider, Katherine Bacon; Kruzel, Deborah
Fonte: MIT - Massachusetts Institute of Technology Publicador: MIT - Massachusetts Institute of Technology
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7.02 and 7.021 require simultaneous registration. Application of experimental techniques in biochemistry, microbiology, and cell biology. Emphasizes integrating factual knowledge with understanding the design of experiments and data analysis to prepare the students for research projects. Concurrent registration with 7.03 or 7.05 is recommended. 12 units may be applied to the General Institute Laboratory Requirement. Instruction and practice in written communication provided. From the course home page: Course Description 7.02 is a laboratory course introducing you to experimental techniques in microbiology, biochemistry, and cell biology. 7.02 emphasizes integrating factual knowledge with understanding the design of experiments and data analysis. The course is divided into four modules: Genetics (GEN) Protein Biochemistry (PBC) Recombinant DNA Methods (RDM) Development (DEV) Each model introduces different experimental techniques and approaches. Although the techniques used in these modules may appear different, many of the underlying theoretical concepts are similar. The skills you learn in 7.02 will be very important should you later enter any research environment, or go on to graduate or medical school.

‣ Características agronômicas de genótipos de bananeira em três ciclos de produção em Rio Branco, AC.

OLIVEIRA, T. K. de; LESSA, L. S.; SILVA, S. de O. e; OLIVEIRA, J. P. de.
Fonte: Pesquisa Agropecuária Brasileira, Brasília, v. 43, n. 8, p. 1003-1010, ago. 2008. Publicador: Pesquisa Agropecuária Brasileira, Brasília, v. 43, n. 8, p. 1003-1010, ago. 2008.
Tipo: Artigo em periódico indexado (ALICE)
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O objetivo deste trabalho foi avaliar características agronômicas de genótipos de bananeira, em três ciclos de produção. O experimento foi realizado em blocos ao acaso, com parcelas subdivididas e cinco repetições, em Rio Branco, AC. Os genótipos Preciosa, Japira, Pacovan Ken, Pacovan, PA42-44, Prata-anã, ST12-31, FHIA 02, Nanicão, Grande Naine, Calipso, Ambrósia e Bucaneiro foram avaliados quanto aos caracteres: altura da planta; diâmetro do pseudocaule; número de folhas vivas na floração e na colheita; ciclo de formação do cacho da floração à colheita; número de pencas por cacho; massa do fruto e do cacho; e produtividade. As cultivares Preciosa e Pacovan Ken apresentaram as maiores alturas, e os híbridos PA42-44 e FHIA 02, e as cultivares Prata-anã, Nanicão e Grande Naine, o porte mais baixo. As cultivares Nanicão, Ambrósia, Bucaneiro, Pacovan, Prata-anã e Grande Naine e o híbrido ST12-31 apresentaram os menores números de folhas vivas na colheita. A cultivar Preciosa e o híbrido FHIA 02 foram os mais produtivos. Os híbridos PA42-44 e FHIA 02 apresentam características agronômicas promissoras e podem ser incorporados ao sistema produtivo, assim como os híbridos de Pacovan, já adaptados e recomendados para o cultivo no Estado do Acre.; 2008

‣ Mapping of the anti-adenoviral cytotoxic T cell response mediated by HLA-A*01, -A*02, and -A*24; Kartierung der von HLA-A*01, -A*02 und -A*24 vermittelten anti-adenoviralen zytotoxischen T-Zellantwort

Mester, Gabor
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
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Human adenovirus infections are a serious threat to immunocompromised individuals, especially stem cell transplantation patients, and a considerable cause of mortality. Although the T cell immune response seems to be essential for the control and clearance of the disease and correlates with a positive course of disease, few adenoviral CD8 T cell epitopes have been identified and their clinical relevance has remained largely unknown. This study was therefore aimed at applying the strategy of reverse immunology and mapping the cytotoxic T cell epitopes from adenovirus for three important HLA allotypes. The main focus was the identification of immunodominant epitopes that elicit T cell responses in most infected hosts of the appropriate HLA genotype. Therefore, the SYFPEITHI algorithm was applied to predict likely epitopes from the primary sequences of three proteins (hexon, pVIII, E1A) of two viral strains (Ad2, Ad5). Candidate epitopes were synthesized as peptides and the PBMCs of healthy donors examined for memory T cell responses specific to them. For this purpose specific T cells were amplified before testing in order to recognize weak responses difficult to detect ex vivo. The HLA restriction of the identified epitopes was verified by testing donors with and without the supposed allotype and by tetramer staining. Specific cell populations were further characterized phenotypically and functionally. For each of the three allotypes one immunodominant epitope could be identified...

‣ HIV Subtype Influences HLA-B*07:02-Associated HIV Disease Outcome

Kløverpris, Henrik N.; Adland, Emily; Koyanagi, Madoka; Stryhn, Anette; Harndahl, Mikkel; Matthews, Philippa C.; Shapiro, Roger; Walker, Bruce D.; Ndung'u, Thumbi; Brander, Christian; Takiguchi, Masafumi; Buus, Søren; Goulder, Philip
Fonte: Mary Ann Liebert, Inc. Publicador: Mary Ann Liebert, Inc.
Tipo: Artigo de Revista Científica
Publicado em 01/05/2014 Português
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Genetic polymorphisms within the MHC encoding region have the strongest impact on HIV disease progression of any in the human genome and provide important clues to the mechanisms of HIV immune control. Few analyses have been undertaken of HLA alleles associated with rapid disease progression. HLA-B*07:02 is an HLA class I molecule that is prevalent in most populations worldwide and that has previously been consistently linked to accelerated disease progression in B-clade infection. This study investigates the observation that HLA-B*07:02 is not associated with a high viral setpoint in C-clade infection. We examine the hypothesis that this clade-specific difference in association with disease outcome may be related to distinct targeting of CD8+ T cell epitopes. We observed that C-clade-infected individuals with HLA-B*07:02 target a broader range of Gag epitopes, and to higher magnitudes, than do individuals infected with B-clade infection. In particular, a novel p17-Gag (Gag22-30, RPGGKKHYM) epitope is targeted in >50% of HLA-B*07:02-positive C-clade-infected individuals but clade-specific differences in this epitope result in nonimmunogenicity in B-clade infection. Only the C-clade p24-Gag “GL9” (Gag355-363, GPSHKARVL) epitope-specific CD8+ T cell response out of 16 studied was associated with a low viral setpoint. Although this epitope was also targeted in B-clade infection...

‣ HOI-02 induces apoptosis and G2-M arrest in esophageal cancer mediated by ROS

Zhang, C; Liu, K; Yao, K; Reddy, K; Zhang, Y; Fu, Y; Yang, G; Zykova, T A; Shin, S H; Li, H; Ryu, J; Jiang, Y-n; Yin, X; Ma, W; Bode, A M; Dong, Z; Dong, Z
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
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Reactive oxygen species (ROS) are chemically reactive molecules that perform essential functions in living organisms. Accumulating evidence suggests that many types of cancer cells exhibit elevated levels of ROS. Conversely, generation of ROS has become an effective method to kill cancer cells. (E)-3-hydroxy-3-(4-(4-nitrophenyl)-2-oxobut-3-en-1-yl) indolin-2-one, which is an NO2 group-containing compound designated herein as HOI-02, generated ROS and, in a dose-dependent manner, decreased esophageal cancer cell viability and inhibited anchorage-independent growth, followed by apoptosis and G2-M arrest. Moreover, results of an in vivo study using a patient-derived xenograft mouse model showed that HOI-02 treatment suppressed the growth of esophageal tumors, without affecting the body weight of mice. The expression of Ki-67 was significantly decreased with HOI-02 treatment. In addition, the phosphorylation of c-Jun, and expression of p21, cleaved caspase 3, and DCFH-DA were increased in the HOI-02-treated group compared with the untreated control group. In contrast, treatment of cells with (E)-3-(4-(4-aminophenyl)-2-oxobut-3-en-1-yl)-3-hydroxyindolin-2-one, which is an NH2 group-containing compound designated herein as HOI-11, had no effect. Overall...

‣ O impacto da Diabetes Mellitus Tipo 02 no estilo de vida antes é pós-diagnóstico da doença

Araújo, Cássia Rejane Cardoso
Fonte: Centro Universitário de Brasília Publicador: Centro Universitário de Brasília
Tipo: Artigo de Revista Científica
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A Síndrome do Diabetes Mellitus tipo 02 é uma desordem metabólica que resulta na inabilidade do corpo em responder apropriadamente à insulina. Esse fenômeno é denominado de resistência insulínica. É a doença metabólica mais comum e apresenta serias implicações na qualidade de vida dos pacientes em decorrência das suas complicações microvasculares e macrovasculares. O Diabetes Mellitus tipo 02 está aumentando de forma exponencial, adquirindo características epidêmicas em vários países, particularmente nos países em desenvolvimento. O objetivo deste estudo foi conhecer o portador da Síndrome do Diabetes Mellitus tipo 02 para verificar o impacto da doença no estilo de vida antes do diagnostico e pós diagnostico para analisar sua relação com as variáveis social, ambiental, familiar, laboral e a adesão ao tratamento por diversas formas, seja ele tratamento medicamento, tratamento não medicamentoso, dieta alimentar e adesão a exercícios físicos para controlar a doença e evitar complicações. As informações do presente estudo foram colhidas por meio de entrevistas. Participaram desse estudo 03 pacientes diagnosticados com a síndrome do Diabetes Mellitus tipo 02, todos os participantes são adultos, com idades entre 49 a 59 anos...

‣ Cosmic ray propagation and dark matter in light of the latest AMS-02 data

Jin, Hong-Bo; Wu, Yue-Liang; Zhou, Yu-Feng
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
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The AMS-02 experiment is measuring the high energy charged cosmic rays with unprecedented accuracy. We explore the possibility of determining the cosmic-ray propagation models using the AMS-02 data $alone$. A global Bayesian analysis of the constraints on the cosmic-ray propagation models from the latest AMS-02 data on the Boron to Carbon nuclei flux ratio and proton flux is performed, with the assumption that the primary nucleon source is a broken power law in rigidity. The ratio of the diffusion coefficient $D_{0}$ to the diffusive halo height $Z_{h}$ is found to be determined with high accuracy $D_{0}/Z_{h}\simeq 2.00\pm0.07\text{cm}^{2}\text{s}^{-1}\text{kpc}^{-1}$. The best-fit value of the halo width is $Z_{h}\simeq 3.3$ kpc with uncertainty less than $50\$. As a consequence, the typical uncertainties in the positron fraction is within a factor of two, and that in the antiproton flux is within an order of magnitude. Both of them are significantly smaller than that from the analyses prior to AMS-02. Taking into account all the uncertainties and correlations in the propagation parameters we derive conservative upper limits on the cross sections for DM annihilating into various standard model final states from the current PAMELA antiproton data. We also investigate the reconstruction capability of the future AMS-02 antiproton data on the DM properties. The result shows that for DM particles lighter than 100 GeV and with typical thermal annihilation cross section...

‣ Fluctuating defects in the incipient relaxor K$_{1-x}$Li$_x$TaO$_3$ (x=0.02)

Stock, C.; Gehring, P. M.; Xu, G.; Lamago, D.; Reznik, D.; Russina, M.; Wen, J.; Boatner, L. A.
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Publicado em 20/05/2015 Português
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We report neutron scattering measurements of the structural correlations associated with the apparent relaxor transition in K$_{1-x}$Li$_x$TaO$_3$ for $x=0.02$ (KLT(0.02)). This compound displays a broad and frequency-dependent peak in the dielectric permittivity, which is the accepted hallmark of all relaxors. However, no evidence of elastic diffuse scattering or any soft mode anomaly is observed in KLT(0.02) [J. Wen et al., Phys. Rev. B 78, 144202 (2008)], a situation that diverges from that in other relaxors such as PbMg$_{1/3}$Nb$_{2/3}$O$_3$. We resolve this dichotomy by showing that the structural correlations associated with the transition in KLT(0.02) are purely dynamic at all temperatures, having a timescale on the order of $\sim$THz. These fluctuations are overdamped, non-propagating, and spatially uncorrelated. Identical measurements made on pure KTaO$_3$ show that they are absent (within experimental error) in the undoped parent material. They exhibit a temperature dependence that correlates well with the dielectric response, which suggests that they are associated with local ferroelectric regions induced by the Li$^+$ doping. The ferroelectric transition that is induced by the introduction of Li$^+$ cations is therefore characterized by quasistatic fluctuations...

‣ Identification of Light Cosmic-Ray Nuclei with AMS-02

Tomassetti, Nicola; Oliva, Alberto
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Publicado em 30/10/2015 Português
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AMS-02 is a wide acceptance (0.5 m2 sr) and long duration (up to 20 years) magnetic spectrometer operating onboard the International Space Station since May 2011. Its main scientific objectives are the indirect research of Dark Matter, searches of primitive Anti-Matter and the precise measurement of the Cosmic-Ray (CR) spectra. Among charged CR species, AMS-02 will be able to measure relative abundances and absolute fluxes of CRs nuclei from Hydrogen up to at least Iron (Z = 26) in a kinetic energy range from hundreds MeV to TeV per nucleon. The high statistics measurement of the chemical composition of CRs in this extended energy range will reveal new insights about the CRs life in the Galaxy, from their origin to the propagation in the interstellar medium, giving new constraints to astrophysical models of Galactic CRs. The nucleus absolute charge, Z, is measured several times along the trajectory of the particle inside AMS-02 using different detection techniques: in the 9 planes of the Silicon Tracker, in the 4 layers of scintillator counters of the Time-of-Flight system (TOF), in the Ring Imaging Cherenkov Counter (RICH) as well as in the 20 layers of Transition Radiation Detector (TRD) and in the upper layers of the Electromagnetic Calorimeter (ECAL). The combination of the redundant measurements delivered by the tracking system and by the TOF allows an accurate discrimination between chemical elements. The charge measurements in the detectors on top of AMS...