Últimos itens adicionados do Acervo: Biblioteca Digital da Produção Intelectual e Científica da Unicamp

A Biblioteca Digital da Produção Intelectual e Científica da Unicamp tem por objetivo reunir e disseminar a produção intelectual, acadêmica e cultural da universidade e preservar sua memória institucional, além de contribuir para ampliar a visibilidade da instituição e dos seus pesquisadores em âmbito nacional e internacional.

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‣ Local Neurotoxicity and Myotoxicity Evaluation of Cyclodextrin Complexes of Bupivacaine and Ropivacaine

Saia Cereda, Cintia Maria; Tofoli, Giovana Radomille; Maturana, Luiz Gabriel; Pierucci, Amauri; Soares Nunes, Lazaro Alessandro; Franz-Montan, Michelle; Rodrigues de Oliveira, Alexandre Leite; Arana, Sarah; de Araujo, Daniele Ribeiro; de Paula, Eneida
Fonte: Lippincott Williams & Wilkins; Philadelphia Publicador: Lippincott Williams & Wilkins; Philadelphia
Tipo: Artigo de Revista Científica
Português
BACKGROUND: Bupivacaine (BVC) and ropivacaine (RVC) are local anesthetics widely used in surgical procedures. In previous studies, inclusion complexes of BVC or RVC in hydroxypropyl-beta-cyclodextrin (HP-beta-CD) increased differential nervous blockade, compared to the plain anesthetic solutions. In this study we evaluated the local neural and muscular toxicity of these new formulations containing 0.5% BVC or RVC complexed with HP-beta-CD (BVCHP-beta-CD and RVCHP-beta-CD). METHODS: Schwann cell viability was assessed by determination of mitochondrial dehydrogenese activity, and histopathological evaluation of the rat sciatic nerve was used to identify local neurotoxic effects (48 hours and 7 days after the treatments). Evaluations of serum creatine kinase levels and the histopathology of rat gastrocnemius muscle (48 hours after treatment) were also performed. RESULTS: Schwann cell toxicity evaluations revealed no significant differences between complexed and plain local anesthetic formulations. However, use of the complexed local anesthetics reduced serum creatine kinase levels 5.5-fold, relative to the plain formulations. The differences were significant at P < 0.05 (BVC) and P < 0.01 (RVC). The histopathological muscle evaluation showed that differences between groups treated with local anesthetics (BVC or RVC) and their respective complexed formulations (BVCHP-beta-CD or RVCHP-beta-CD) were significant (P < 0.05). CONCLUSIONS: We concluded that the new formulations presented a lower myotoxicity and a similar cytotoxic effect when compared to plain local anesthetic solutions. (Anesth Analg 2012;115:1234-41); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

‣ Proteomic profiling of skeletal muscle in an animal model of overtraining

Gandra, Paulo Guimaraes; Valente, Richard Hemmi; Perales, Jonas; Fonseca Pacheco, Antonio Guilherme; Macedo, Denise Vaz
Fonte: Wiley-Blackwell; Hoboken Publicador: Wiley-Blackwell; Hoboken
Tipo: Artigo de Revista Científica
Português
Excessive training (i.e. overtraining, OT) may result in underperformance, which can be characterized by the time needed to re-establish performance (i.e. functional overreaching (FOR), nonfunctional overreaching, OT syndrome). The present study is an initial screening for proteins presenting altered abundance in the red (RG) and white (WG) portions of the gastrocnemius muscle from rats submitted to an OT protocol that induced FOR. In the RG, compared to the nontrained control, FOR demonstrated an increased abundance of proteins normally related to adaptation to endurance training (e.g. proteins of oxidative phosphorylation complexes, proteins related to lipid metabolism, antioxidants, and chaperones). In the WG, spots identified as mitochondrial aconitase and a component of the succinate dehydrogenase complex were downregulated in FOR, as were proteins related to myofibril stabilization; these latter were upregulated in the RG. This initial study shows that skeletal muscles with different fiber-type compositions respond differently to an OT period. Also, it is likely that actin-interacting proteins have an important role in muscle adaptation to endurance exercise.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

‣ Purification and biochemical properties of a Kunitz-type trypsin inhibitor from Entada acaciifolia (Benth.) seeds

Ramalho de Oliveira, Caio Fernando; Vasconcelos, Ilka Maria; Aparicio, Ricardo; Machado Freire, Maria das Gracas; Baldasso, Paulo Aparecido; Marangoni, Sergio; Rodrigues Macedo, Maria Ligia
Fonte: Elsevier; Oxford Publicador: Elsevier; Oxford
Tipo: Artigo de Revista Científica
Português
A new trypsin inhibitor (EATI) was isolated from Entada acaciifolia (Benth.) seeds. EATI is a competitive inhibitor with a molecular mass of 20 kDa and an inhibition stoichiometry of 1:1 for bovine trypsin. The dissociation constant (K-i) calculated was 1.75 nmol/L, displaying a high affinity between enzyme and inhibitor. Both Native PAGE and RP-HPLC revealed that EATI is composed of four isoinhibitors that share the amino acid composition and the amino-terminal sequence homolog to Kunitz-type inhibitors. EATI is stable to denaturation by heat (up to 70 degrees C), pH (2-10), urea (8 mol/L) and its inhibitory activity was unaltered in different concentrations of DTT (up to 100 mmol/L). CD analysis revealed that EATI in reduced form underwent structural modifications associated with a decrease in thermal and pH stabilities, suggesting that their disulfide bonds are not involved in the structuring of its reactive site, but are important for maintenance of its conformational stability. This behavior makes EATI one of the few inhibitors described in the literature with high DTT resistance. (C) 2012 Elsevier Ltd. All rights reserved.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

‣ Sufentanil-2-hydroxypropyl-beta-cyclodextrin inclusion complex for pain treatment: Physicochemical, cytotoxicity, and pharmacological evaluation

Volobuef, Cristiane; Moraes, Carolina M.; Nunes, Lazaro A. S.; Cereda, Cintia M. S.; Yokaichiya, Fabiano; Franco, Margareth K. K. D.; Braga, Angelica F. A.; De Paula, Eneida; Tofoli, Giovana Radomille; Fraceto, Leonardo F.; De Araujo, Daniele R.
Fonte: Wiley-Blackwell; Hoboken Publicador: Wiley-Blackwell; Hoboken
Tipo: Artigo de Revista Científica
Português
Sufentanil (SUF) is a synthetic analgesic opioid widely used for the management of acute and chronic pain. This drug was complexed with 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) and the physicochemical characterization, in vitro/ex vivo toxicity assays, and pharmacological evaluation were performed. Differential scanning calorimetry, Fourier transform infrared spectroscopy (FTIR) analysis, and X-ray powder diffraction showed the formation and the morphology of the complex. Nuclear magnetic resonance afforded data regarding inclusion complex stoichiometry (1:1) with an association binding constant (Ka) value of 515.2 +/- 1.2?M-1 between SUF and HP-beta-CD. Complexation with HP-beta-CD protected SUF from light exposure and increased its photostability. Release kinetics revealed a decrease in SUF release rate (Krel = 7.05 +/- 0.52 and 5.61 +/- 0.39?min-1/2 for SUFHP-beta-CD and SUF, respectively) and reduced hemolytic or myotoxic effects after complexation. Time course of tail-flick test showed that the duration of analgesia induced by SUF (150.0 +/- 34.6?min) was significantly increased (p < 0.001) after complexation with HP-beta-CD (355.7 +/- 47.2?min) when injected at the same dose (1 mu g kg-1), prolonging the duration of analgesia after intramuscular administration and representing an alternative on the development of effective and safe drug-delivery system for opioid analgesics. (C) 2012 Wiley Periodicals...

‣ NATURAL REGENERATION IN ABANDONED FIELDS FOLLOWING INTENSIVE AGRICULTURAL LAND USE IN AN ATLANTIC FOREST ISLAND, BRAZIL

Silvestrini, Milene; Cysneiro, Airton de Deus; Lima, Aline Lopes; Veiga, Larissa Giorgeti; Isernhagen, Ingo; Tamashiro, Jorge Yoshio; Gandolfi, Sergius; Rodrigues, Ricardo Ribeiro
Fonte: Univ Federal Vicosa; Vicosa Publicador: Univ Federal Vicosa; Vicosa
Tipo: Artigo de Revista Científica
Português
The time required to regrowth a forest in degraded areas depends on how the forest is removed and on the type of land use following removal. Natural regeneration was studied in abandoned old fields after intensive agricultural land use in areas originally covered by Brazilian Atlantic Forests of the Anchieta Island, Brazil in order to understand how plant communities reassemble following human disturbances as well as to determine suitable strategies of forest restoration. The fields were classified into three vegetation types according to the dominant plant species in: 1) Miconia albicans (Sw.) Triana (Melastomataceae) fields, 2) Dicranopteris flexuosa (Schrader) Underw. (Gleicheniaceae) thickets, and 3) Gleichenella pectinata (Willd.) Ching. (Gleicheniaceae) thickets. Both composition and structure of natural regeneration were compared among the three dominant vegetation types by establishing randomly three plots of 1 x 3 m in five sites of the island. A gradient in composition and abundance of species in natural regeneration could be observed along vegetation types from Dicranopteris fern thickets to Miconia fields. The gradient did not accurately follow the pattern of spatial distribution of the three dominant vegetation types in the island regarding their proximity of the remnant forests. A complex association of biotic and abiotic factors seems to be affecting the seedling recruitment and establishment in the study plots. The lowest plant regeneration found in Dicranopteris and Gleichenella thickets suggests that the ferns inhibit the recruitment of woody and herbaceous species. Otherwise...

‣ A new species of Paralychnophora (Asteraceae: Vernonieae), and comments on the identity of Paralychnophora bicolor

Loeuille, Benoit; Semir, Joao; Pirani, Jose Rubens
Fonte: Springer; New York Publicador: Springer; New York
Tipo: Artigo de Revista Científica
Português
The type of Paralychnophora bicolor was found to represent another species recently described as P. santosii. The misapplication of the name P. bicolor is reviewed. Also, a new species is here proposed, P. glaziouana, for plants previously identified as P. bicolor, since the only available name, P. schwackei, is an illegitimate combination. A key to Paralychnophora is given.; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

‣ Pancreatic islets from dexamethasone-treated rats show alterations in global gene expression and mitochondrial pathways

Roma, Leticia P.; Souza, Kleber L. A.; Carneiro, Everardo M.; Boschero, Antonio C.; Bosqueiro, Jose R.
Fonte: General Physiol And Biophysics; Bratislava Publicador: General Physiol And Biophysics; Bratislava
Tipo: Artigo de Revista Científica
Português
Chronic administration of glucocorticoids (GC) leads to characteristic features of type 2 diabetes in mammals. The main action of dexamethasone in target cells occurs through modulation of gene expression, although the exact mechanisms are still unknown. We therefore investigated the gene expression profile of pancreatic islets from rats treated with dexamethasone using a cDNA array screening analysis. The expression of selected genes and proteins involved in mitochondria] apoptosis was further analyzed by PCR and immunoblotting. Insulin, triglyceride and free fatty acid plasma levels, as well as glucose-induced insulin secretion, were significantly higher in dexamethasone-treated rats compared with controls. Out of 1176 genes, 60 were up-regulated and 28 were down-regulated by dexamethasone treatment. Some of the modulated genes are involved in apoptosis, stress response, and proliferation pathways. RT-PCR confirmed the cDNA array results for 6 selected genes. Bax alpha protein expression was increased, while Bcl-2 was decreased. In vivo dexamethasone treatment decreased the mitochondrial production of NAD(P)H, and increased ROS production. Concluding, our data indicate that dexamethasone modulates the expression of genes and proteins involved in several pathways of pancreatic-islet cells...

‣ Age-dependent changes in rat lacrimal gland anti-oxidant and vesicular related protein expression profiles

Batista, Thiago Martins; Tomiyoshi, Lilian Midori; Dias, Ana Carolina; Roma, Leticia Prates; Modulo, Carolina Maria; Malki, Leonardo Tannus; Machado Filho, Elisio Bueno; Deminice, Rafael; Jordao, Alceu Afonso, Jr.; Cunha, Daniel A.; Rocha, Eduardo Melani
Fonte: Molecular Vision; Atlanta Publicador: Molecular Vision; Atlanta
Tipo: Artigo de Revista Científica
Português
Purpose: Anti-oxidation and exocytosis are important for maintaining exocrine tissue homeostasis. During aging, functional and structural alterations occur in the lacrimal gland (LG), including oxidative damage to proteins, lipids, and DNA. The aims of the present study were to determine in the aging LG: a) the effects of aging on LG structure and secretory activity and b) changes in the expression of oxidative stress markers. Methods: To address these goals, tear secretion composition and corneal impression cytology were compared between male Wistar rats of 2 (control) and 24 (aged) months. LG morphology and the expression levels of vitamin E and malonaldehyde (MDA) were evaluated to determine the anti-oxidant activity and lipid peroxidation, respectively. RT-PCR and western blot analysis were used for the analysis of Ras related in brain GTPase protein (Rab) and soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins of the secretory machinery (i.e.; Rab 3d, Rab 27, vesicle-associated membrane protein-2 (Vamp-2), and syntaxin). Results: Histological analysis of aged rats revealed a higher frequency of corneal epithelia metaplasia. In the acinar cells, organelles underwent degeneration, and lipofucsin-like material accumulated in the cytoplasm along with declines in the anti-oxidant marker vitamin E. Rab3d and Rab27b mRNA levels fell along with Rab3d protein expression...

‣ The Role of Dyslipidemia on Ocular Surface, Lacrimal and Meibomian Gland Structure and Function

Modulo, Carolina Maria; Machado Filho, Elisio Bueno; Malki, Leonardo Tannus; Dias, Ana Carolina; de Souza, Jane Cristina; Oliveira, Helena C. F.; Jorge, Italo Cade; Santos Gomes, Isabele Beserra; Meyrelles, Silvana S.; Rocha, Eduardo Melani
Fonte: Informa Healthcare; London Publicador: Informa Healthcare; London
Tipo: Artigo de Revista Científica
Português
Purpose: Dyslipidemia is characterized by high lipid blood levels that are risk factors for cardiovascular diseases, which are leading causes of death. However, it is unclear whether dyslipidemia is a cause of the dry eye syndrome (DES). Therefore we determined in transgenic mice models of dyslipidemia, whether there is an association with DES development. Methods: Dyslipidemic models included male and female adult mice overexpressing apolipoprotein CIII (Apo CIII), LDL receptor knockout (LDLR-KO) and ApoE knockout (ApoE-KO). They were compared with age-and gender-matched C57BL/6 mice. Ocular health was evaluated based on corneal slit lamp assessment, phenol red thread test (PRT) and impression cytology. Blood lipid profiles and histology of meibomian and lacrimal glands were also evaluated. Effects of high-fat diet and aging were observed in LDLR-KO and ApoCIII strains, respectively. Results: Body weight and lacrimal gland weight were significantly higher in male mice compared to females of the same strain (P < 0.05). Body weight was significantly lower in LDLRKO mice receiving high lipid diet compared to their controls (P = 0.0043). ApoE-KO were hypercholesterolemic and ApoCIII hypertriglyceridemic while LDLR-KO showed increases in both parameters. The PRT test was lower in male LDLR-KO mice with high-fat diet than control mice with standard diet (P = 0.0273). Aging did not affect lacrimal structural or functional parameters of ApoCIII strain. Conclusions: DES development is not solely dependent on dyslipidemia in relevant mice models promoting this condition. On the other hand...

‣ AGE INFLUENCE ON THE HEART RATE BEHAVIOR ON THE REST-EXERCICIO TRANSITION: AN ANALYSIS BY DELTAS AND LINEAR REGRESSION

Beltrame, Thomas; Karsten, Marlus; Traina Chacon-Mikahil, Mara Patrcia; Madruga, Vera Aparecida; da Silva, Ester; Borghi-Silva, Audrey; Gallo Junior, Lourenco; Catai, Aparecida Maria
Fonte: Soc Brasileira Med Esporte; Sao Paulo Publicador: Soc Brasileira Med Esporte; Sao Paulo
Tipo: Artigo de Revista Científica
Português
Background: Changes in heart rate during rest-exercise transition can be characterized by the application of mathematical calculations, such as deltas 0-10 and 0-30 seconds to infer on the parasympathetic nervous system and linear regression and delta applied to data range from 60 to 240 seconds to infer on the sympathetic nervous system. The objective of this study was to test the hypothesis that young and middle-aged subjects have different heart rate responses in exercise of moderate and intense intensity, with different mathematical calculations. Methods: Seven middle-aged men and ten young men apparently healthy were subject to constant load tests (intense and moderate) in cycle ergometer. The heart rate data were submitted to analysis of deltas (0-10, 0-30 and 60-240 seconds) and simple linear regression (60-240 seconds). The parameters obtained from simple linear regression analysis were: intercept and slope angle. We used the Shapiro-Wilk test to check the distribution of data and the "t" test for unpaired comparisons between groups. The level of statistical significance was 5%. Results: The value of the intercept and delta 0-10 seconds was lower in middle age in two loads tested and the inclination angle was lower in moderate exercise in middle age. Conclusion: The young subjects present greater magnitude of vagal withdrawal in the initial stage of the HR response during constant load exercise and higher speed of adjustment of sympathetic response in moderate exercise.

‣ Functional properties of gelatin-based films containing Yucca schidigera extract produced via casting, extrusion and blown extrusion processes: A preliminary study

Andreuccetti, Caroline; Carvalho, Rosemary A.; Galicia-Garcia, Tomas; Martinez-Bustos, Fernando; Gonzalez-Nunez, Ruben; Grosso, Carlos R. F.
Fonte: Elsevier; Oxford Publicador: Elsevier; Oxford
Tipo: Artigo de Revista Científica
Português
Gelatin-based films containing both Yucca schidigera extract and low concentrations of glycerol (0.25-8.75 g per 100 g protein) were produced by extrusion (EF) and characterized in relation to their mechanical properties and moisture content. The formulations that resulted in either larger or smaller elongation values were used to produce films via both blown extrusion (EBF) and casting (CF) and were characterized with respect to their mechanical properties, water vapor permeability, moisture content, solubility, morphology and infrared spectroscopy. The elongation of the EF films was significantly higher than that of the CF and EBF films. The transversal section possessed a compact, homogeneous structure for all of the films studied. The solubility of the films (36-40%) did not differ significantly between the different processes evaluated. The EBF films demonstrated lower water vapor permeability (0.12 g mm m-(2) h(-1) kPa(-1)) than the CF and EF films. The infrared spectra did not indicate any strong interactions between the added compounds. Thermoplastic processing of the gelatin films can significantly increase their elongation; however, a more detailed assessment and optimization of the extrusion conditions is necessary, along with the addition of partially hydrophobic compounds...

‣ Effects of whey protein and casein plus leucine on diaphragm the mTOR pathway of sedentary, trained rats

Lollo, P. C. B.; Silva, L. B. C.; Batista, T. M.; Morato, P. N.; Moura, C. S.; Cruz, A. G.; Faria, J. A. F.; Carneiro, E. M.; Amaya-Farfan, J.
Fonte: Elsevier; Amsterdam Publicador: Elsevier; Amsterdam
Tipo: Artigo de Revista Científica
Português
The whey proteins are rich sources of leucine and affect mTOR-mediated skeletal muscle protein synthesis. The present authors wished to investigate the dose-response effect of chronic supplementation of the whey proteins (WP) with leucine on the anabolic pathway proteins mTOR and p70S6K in the diaphragm of sedentary and exercised Wistar rats. Ninety-six weanling male Wistar rats were divided into eight groups and fed for 30 day diets containing either casein or WP, with increasing levels (0, 3, 4.5 and 6% of the diet) of leucine. A parallel set of eight groups was exercised for comparison. Serum uric acid, creatinine, glucose, AST, ALT, CK, LDH and cholesterol, were determined by standard methods, and mTOR and p70S6K, using the Western blot analysis. Leucine supplementation increased both, mTOR and p7056K in the diaphragm in a dose-dependent way, independent of the type of dietary protein. These changes produced no significant increase in either diaphragm or protein mass. (C) 2012 Elsevier Ltd. All rights reserved.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

‣ Effect of combined treatment of hydrolysis and polymerization with transglutaminase on beta-lactoglobulin antigenicity

Sabadin, Isabele Serimarco; Villas-Boas, Mariana Battaglin; Zollner, Ricardo de Lima; Netto, Flavia Maria
Fonte: Springer; New York Publicador: Springer; New York
Tipo: Artigo de Revista Científica
Português
The effect of combined treatments of hydrolysis with different proteases, and subsequent polymerization with transglutaminase on the antigenic activity of beta-Lg was studied. For the hydrolysis of beta-Lg using Alcalase, Neutrase or bromelain, the reaction conditions were 3 % beta-Lg and enzyme:substrate 25 U g(-1) of protein, as was defined using factorial study. Under these conditions, the degree of hydrolysis (DH) of the hydrolysates was 12.6 % when obtained with Alcalase and approximately 4 % with Neutrase or bromelain. Post-hydrolysis polymerization did not result in an increase in molecular mass of the protein, but these samples presented a lower DH, determined by trinitrobenzenosulfonic acid (TNBS) method, suggesting that polymerization had occurred. Hydrolysis with the three enzymes reduced the beta-Lg antigenicity, as evaluated by ELISA and immunoblotting analyses. The IgE-binding responses were practically null (< 9 mu g mL(-1)), 22.82 and 55.73 mu g mL(-1) towards the hydrolysates obtained with Alcalase, bromelain, and Neutrase, respectively. The post-hydrolysis polymerization increased or had no significant effect (P a parts per thousand yen 0.05) on the antigenic response of the hydrolysates.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

‣ Changes of Large Molecular Weight Hyaluronan and Versican in the Mouse Pubic Symphysis Through Pregnancy

Rosa, Renata Giardini; Akgul, Yucel; Joazeiro, Paulo Pinto; Mahendroo, Mala
Fonte: Soc Study Reproduction; Madison Publicador: Soc Study Reproduction; Madison
Tipo: Artigo de Revista Científica
Português
During pregnancy, the mouse pubic symphysis undergoes expansion and remodeling resulting in formation of a flexible and elastic interpubic ligament allowing passage of a term fetus. In the current study, we sought to identify and characterize components of the extracellular matrix that likely play an important role in elongation and flexibility of the interpubic ligament during parturition. Mouse pubic symphyses and interpubic ligaments collected at time points during pregnancy and postpartum were utilized to evaluate collagen type, collagen content, processing and solubility, matricellular protein, and proteoglycan expression and quantitative assessment of all glycosaminoglycans. These studies revealed increased gene expression for hyaluronan synthase 1, hyaluronan synthase 2, and versican on Gestation Day 18 as well as a decline in protein expression for the versican-degrading protease a disintegrin-like and metalloprotease with thrombospondin type 1 (ADAMTS1) motif. These findings suggest that the primary mediators of increased elongation and flexibility of the interpubic ligament at term result from increased synthesis and reduced metabolism of viscoelasticity-promoting molecules such as high molecular weight hyaluronan and versican.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

‣ Delta-Like Ligand (DLL)1 Expression in Early Mouse Decidua and Its Localization to Uterine Natural Killer Cells

Degaki, Karina Y.; Chen, Zhilin; Yamada, Aureo T.; Croy, B. Anne
Fonte: Public Library Science; San Francisco Publicador: Public Library Science; San Francisco
Tipo: Artigo de Revista Científica
Português
Uterine vascular changes, critical for pregnancy success, occur at each implant site during endometrial decidualization. Mesometrial decidualization recruits high numbers of angiogenic, uterine Natural Killer (uNK) cells that trigger midpregnancy spiral arterial remodeling. We postulated that uNK cells contribute to early decidual angiogenesis as endothelial-cell extrinsic sources of Delta-like ligand 1 (DLL1), a molecule that induces endothelial tip cell differentiation and orthogonal vascular growth in other tissues. Virgin uteri expressed Dll1 mesometrially and anti-mesometrially and relative expression increased in both anatomic regions as pregnancy progressed. Analyses of transcripts from gd10.5 uNK cells flow sorted on the basis of expression of Dolichos biflorus agglutinin (DBA) lectin revealed that DBA+ but not DBA- uNK cells expressed Dll1. Immunostaining at gd4.5 found DLL1-expressing cells rare. At gd6.5, DBA+ uNK cells at all stages of maturation expressed DLL1. By gd10.5, DLL1 immunoreactivity was strongly expressed by some but not all DBA+ uNK cells and more weakly by DBA- cells. DLL1+ cells were mesometrially stratified and concentrated within central decidua basalis. Our data suggest that uNK cells have the potential to induce endothelial tip cell differentiation and to promote non-planar vascular growth within early decidua basalis.; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

‣ Focal adhesion kinase governs cardiac concentric hypertrophic growth by activating the AKT and mTOR pathways

Clemente, C. F. M. Z.; Xavier-Neto, J.; Costa, A. P. Dalla; Consonni, S. R.; Antunes, J. E.; Rocco, S. A.; Pereira, M. B.; Judice, C. C.; Strauss, B.; Joazeiro, P. P.; Matos-Souza, J. R.; Franchini, K. G.
Fonte: Elsevier; London Publicador: Elsevier; London
Tipo: Artigo de Revista Científica
Português
The heart responds to sustained overload by hypertrophic growth in which the myocytes distinctly thicken or elongate on increases in systolic or diastolic stress. Though potentially adaptive, hypertrophy itself may predispose to cardiac dysfunction in pathological settings. The mechanisms underlying the diverse morphology and outcomes of hypertrophy are uncertain. Here we used a focal adhesion kinase (FAK) cardiac-specific transgenic mice model (FAK-Tg) to explore the function of this non-receptor tyrosine kinase on the regulation of myocyte growth. FAK-Tg mice displayed a phenocopy of concentric cardiac hypertrophy, reflecting the relative thickening of the individual myocytes. Moreover, FAK-Tg mice showed structural, functional and molecular features of a compensated hypertrophic growth, and preserved responses to chronic pressure overload. Mechanistically, FAK overexpression resulted in enhanced myocardial FAK activity, which was proven by treatment with a selective FAK inhibitor to be required for the cardiac hypertrophy in this model. Our results indicate that upregulation of FAK does not affect the activity of Src/ERK1/2 pathway, but stimulated signaling by a cascade that encompasses PI3K, AKT, mTOR, S6K and rpS6. Moreover, inhibition of the mTOR complex by rapamycin extinguished the cardiac hypertrophy of the transgenic FAK mice. These findings uncover a unique role for FAK in regulating the signaling mechanisms that governs the selective myocyte growth in width...

‣ Synaptophysin Expression and Neurotoxic Effects of Some Bothropic Venoms and Toxins

Rocha, Thalita; Ponce-Soto, Luis A.; Marangoni, Sergio; da Cruz-Hoefling, Maria Alice
Fonte: Pergamon-Elsevier Science Ltd; Oxford Publicador: Pergamon-Elsevier Science Ltd; Oxford
Tipo: Artigo de Revista Científica
Português

‣ Differential Expression of Aquaporin 4 in the Hippocampus of Neonate and Adult Rats after Envenoming by Phoneutria nigriventer (Ctenidae, Araneomorphae)

Stavale, Leila Miguel; Soares, Edilene S.; Mendonca, Monique C. P.; da Cruz Hofling, Maria Alice
Fonte: Pergamon-Elsevier Science Ltd; Oxford Publicador: Pergamon-Elsevier Science Ltd; Oxford
Tipo: Artigo de Revista Científica
Português

‣ Regulation of neuronal and endothelial nitric oxide synthase by anabolic-androgenic steroid in skeletal muscles

Fontana, Karina; Rocha, Thalita; da Cruz-Hoefling, Maria Alice
Fonte: F Hernandez; Murcia Publicador: F Hernandez; Murcia
Tipo: Artigo de Revista Científica
Português
Anabolic-androgenic steroids (AAS) and exercise share comparable effects on myogenic differentiation, force development, fiber growth and skeletal muscle plasticity. The participation of nitric oxide synthase (NOS) on these effects was only demonstrated in response to exercise. Using immunohistochemistry and western blotting we examined the effect of AAS on the expression of NOS I and III isoforms in three muscles, distinct metabolically and physiologically: soleus (SOL), tibialis anterioris (TA) and gastrocnemius (GAS). Mice with a lipid profile akin to humans were used. Sedentary mice (Sed-C) or exercised, submitted to six-weeks of aerobic treadmill running (one hour/day, 5 days/week) were administered mesterolone (Sed-M and Ex-M, respectively) or gum arabic (vehicle, Ex-C) during the last three weeks, three alternate days per week. Consistently, The TA showed the strongest labeling and the SOL the weakest with NOS III predominating over NOS I. Mesterolone administered to sedentary mice (Sed-C x Sed-M) significantly upregulated NOS I in TA and SOL and NOS III in all three muscles. Mesterolone administered to exercised mice (Ex-C x Ex-M) upregulated NOS I in all three muscles and NOS III in TA and SOL. The exercise to mesterolone-treated mice (Sed-M x Ex-M) produced a strong increase in NOS I expression in GAS; in contrast it antagonized the mesterolone-induced upregulation of NOS I in TA muscle and NOS III in SOL and GAS. The data show nitric oxide (NO) as a potential signaling mediator of AAS effects in skeletal muscle and that NOS I and NOS III upregulations were muscle phenotype-specific. These may be regarded as an indication of the complex NOS/NO signaling mechanism related with AAS effects vs. metabolic/physiological muscle characteristics.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

‣ AN UNAUTHORIZED BIOGRAPHY OF THE SECOND HEART FIELD AND A PIONEER/SCAFFOLD MODEL FOR CARDIAC DEVELOPMENT

Xavier-Neto, Jose; Trueba, Sylvia Sura; Stolfi, Alberto; Souza, Henrique Marques; Pascoal Sobreira, Tiago Jose; Schubert, Michael; Castillo, Hozana Andrade
Fonte: Elsevier; San Diego Publicador: Elsevier; San Diego
Tipo: Artigo de Revista Científica
Português
The identification of subpharyngeal cardiac precursors has had a strong influence on the way we think about early cardiac development. From this discovery was born the concept of multiple heart fields. Early support for the concept came from gene expression, genetic retrospective fate mapping, and gene targeting studies, which collectively suggested the existence of a second heart field (SHF) on the basis of specific Islet-1 (Isl-1) expression, presence of two cardiac ancestral lineages, and compatible cardiac knockout phenotypes, respectively. A decade after the original studies, support for the SHF concept is dwindling. This is because in all bilaterian models studied, Isl expression in heart progenitors is not SHF-specific, because lineage data are best explained by alternative models including an older, truly ancestral, lineage of cardiac pioneers with unrestricted contribution to all cardiac segments and, finally, because the inflow-to-outflow segmental nature of the early vertebrate peristaltic heart has been reaffirmed with novel, less invasive, methodologies. Altogether, the paradigms derived from the discovery of subpharyngeal cardiac progenitors helped us shift from relatively simple models, which rely predominantly either on patterning...