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‣ Função dos músculos respiratórios em doentes com insuficiência cardíaca esquerda

Carmo, Miguel Mota; Bárbara, Cristina; Ferreira, Teresa; Ferreira, Sara; Rendas, António Bensabat
Fonte: Sociedade Portuguesa de Cardiologia Publicador: Sociedade Portuguesa de Cardiologia
Tipo: Artigo de Revista Científica
Publicado em //2001 Português
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Revista Portuguesa de Cardiologia, 2001; 20 (5):533-545; Objectivo: 0 objectivo deste trabalho foi : estudar a função dos músculos respiratórios em doentes com insuficiência cardíaca esquerda moderada (ICE), e o seu contributo para a génese do cansaço e da dispneia. Concepção do estudo:Estudo prospectivo comparativo entre doentes com ICE e normais. Doentes: Estudámos 10 doentes do sexo masculino, com insuficiência cardíaca esquerda (GI), classe II e III da NYHA, com idade média de 65.6±6.9 anos e 10 controles normais, sem patologia cardio-pulmonar (GII), com idade média de 64.5±4.9 anos. Material e métodos: Utilizános métodos dependentes da vontade com determinações das pressões máximas expiratórias (PME)ao nível da Capacidade Pulmonar Total e inspiratórias (PMI) ao nível da Capacidade Residual Funcional, das pressões através de sniff nasal ·(SNIFF-N) e da pressão de sniff esofágico (SNIFF-E). Determinámos igualmente as pressões transdiafragmáticss (TwPDI). Com os métodos independentes da vontade, utilizando a estimulação magnética cervical do frénico ao nível da CRF determinámos a pressão Twitch esofágica (TwPes), a Twitch gástrica (TwGas)e a Twitch transdiafragmática (TwPDI). Resultados: No que conceme às pressões dependentes da vontade não obtivemos diferenças significativas da PME (cmH2O): GI-136±38; GII-145.5±36.8; p=NS e da PMI (cmH2O):GI-73.720.7; GII 87.1±ll.7; p=NS; Estas foram no entanto inferiores às ohtidas através da técnica do sniff. Obtivemos uma pressão significativamente menor nos doentes com ICE do SNIFF-N (cmH20):GI-87±l0.7;GII-99...

‣ A importância do Spread e das garantias na concessão de crédito às pequenas e médias empresas

Fortunato, Carlos Jorge Chasqueira
Fonte: Universidade da Beira Interior Publicador: Universidade da Beira Interior
Tipo: Dissertação de Mestrado
Publicado em //2013 Português
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A grande maioria das pequenas e médias empresas (PME) depende dos bancos comerciais para obterem financiamento bancário. Como os custos de transacção, o custo de funding e as garantias exigidas pelos bancos limitam o fornecimento de crédito às PME, este estudo analisa os determinantes do custo do financiamento, isto é, do spread aplicado aos empréstimos e das garantias particulares solicitadas aos mutuários no processo de concessão de crédito. Para tal recolheu-se informação relativamente a 18.687 empréstimos concedidos a PME, por um dos maiores bancos portugueses. Os resultados obtidos com base no modelo OLS e com referência aos determinantes do spread indicam que: i) o valor do spread cobrado aumenta sempre que sejam exigidas garantias aos sócios e/ou gerentes; ii) com referência às garantias prestadas pela empresa e pelas SGM obtém-se um efeito contrário, isto é, um decréscimo do valor do spread; iii) para empréstimos de longo prazo e de montantes mais elevados o valor do spread diminui, efeito explicado pela redução dos custos de monitorização dos contratos de crédito; iv) quanto às características do mutuário, as empresas que tenham decréscimos no seu volume de negócios ou que apresentem incumprimentos após a concessão do empréstimo provocam aumentos no spread...

‣ O impacto do novo acordo de Capital de Basileia na concessão de crédito e pequenas e médias empresas

Rocha, Catarina Delgado de Aguilar Botelho
Fonte: Universidade da Beira Interior Publicador: Universidade da Beira Interior
Tipo: Dissertação de Mestrado
Publicado em //2013 Português
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Este estudo tem como objetivo investigar qual o impacto da introdução do Novo Acordo de Basileia II (BII) no montante de crédito concedido às Pequenas e Médias Empresas (PME), em Portugal. Para tal, foi recolhida uma amostra de 56 empréstimos concedidos por uma Instituição financeira (IF), representando o mercado bancário português, entre 1 de janeiro de 2006 e 31 de dezembro de 2012, adotando-se como metodologia um modelo de regressão linear múltipla. Os resultados obtidos evidenciam que a introdução do BII não degradou as condições de acesso ao crédito das PME consideradas no estudo e que o possível “Efeito Basileia II” apenas se verifica nas empresas não classificadas de acordo com o QIS3. Todavia este efeito acentuasse após 1 de janeiro de 2008, dada a forte redução do montante de crédito que é concedido a estas PME.

‣ Produção e purificação de imunoglobulinas Y policlonais anti-Leptospira spp.

Tavares,Tatiane C.F.; Soares,Pollyanna M.; Neves,João H.F.F.; Soares,Mayara M.; F. Junior,Álvaro; Souza,Dayane L.N. de; Ávila,Veridiana M.R.; Lima-Ribeiro,Anna M.C.
Fonte: Colégio Brasileiro de Patologia Animal - CBPA; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Publicador: Colégio Brasileiro de Patologia Animal - CBPA; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2013 Português
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Objetivou-se verificar se galinhas imunizadas com uma solução de Leptospira interrogans inativadas e proteínas de membrana externa do sorovar Hardjo, poderiam produzir anticorpos policlonais específicos anti-leptospiras, detectáveis em testes ELISA. Foram imunizados oito galinhas com 25 semanas de idade, da raça White Leghorn, sendo três imunizadas com uma suspensão de leptospiras inativadas, três com uma solução de proteínas de membrana externa extraída do sorovar Hardjo e duas controle. Coletas de sangue foram realizadas quinzenalmente e de ovos diariamente. A IgY foi purificada a partir da gema dos ovos utilizando para a delipidação o método de diluição em água ácida e a precipitação com sulfato de amônio. Nos testes ELISA realizados para verificar a especificidade da IgY, foi demonstrada a produção de anticorpos anti-Leptospira, tanto no soro quanto nas gemas purificadas. O pico de produção de anticorpos específicos ocorreu na 5º semana após a primeira imunização. Ficou demonstrada a possibilidade da indução da produção de anticorpos específicos em galinhas imunizadas com leptospiras do sorovar Hardjo inativadas, bem como, com proteínas de membrana externa (PME) extraidas desse sorovar. As galinhas imunizadas com uma suspensão de leptospiras inativadas ou com PME de Leptospira interrogans do sorovar Hardjo produziram anticorpos reativos a PME Hardjo detectáves por teste ELISA.

‣ Suculência e solubilização de pectinas em maçãs ‘Gala’, armazenadas em atmosfera controlada, em dois níveis de umidade relativa

Lunardi,Rosangela; Brackmann,Auri; Sestari,Ivan; Zanatta,Jocemar Francisco; Silva,Jorge Adolfo da; Rombaldi,Cesar Valmor
Fonte: Universidade Federal de Santa Maria Publicador: Universidade Federal de Santa Maria
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2004 Português
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O objetivo deste trabalho foi o de avaliar o efeito de umidades relativas (UR) de 96 e 90%, em maçãs ‘Gala’ armazenadas por 8 meses em atmosfera controlada (AC) sobre a perda de suculência, firmeza de polpa, conteúdo de pectina solúvel (PS) e atividade das enzimas pectinametilesterase (PME) e poligalacturonase (PG). A temperatura de armazenamento em AC foi de 0,5°C. Ao final do período em AC e mais 7 dias a 20°C, foram avaliadas a suculência, a firmeza de polpa, o conteúdo de PS e as atividades da PME e PG. A UR na faixa de 96 a 90% não afetou a suculência, a firmeza, o conteúdo de PS e a atividade da PME, mas em 90% de UR ocorreu aumento da atividade da PG, após 8 meses em AC e mais 7 dias a 20°C.

‣ In Vitro Studies of Pharmacodynamic Properties of Vancomycin against Staphylococcus aureus and Staphylococcus epidermidis

Löwdin, E.; Odenholt, I.; Cars, O.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /10/1998 Português
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The bactericidal activities of vancomycin against two reference strains and two clinical isolates of Staphylococcus aureus and Staphylococcus epidermidis were studied with five different concentrations ranging from 2× to 64× the MIC. The decrease in the numbers of CFU at 24 h was at least 3 log10 CFU/ml for all strains. No concentration-dependent killing was observed. The postantibiotic effect (PAE) was determined by obtaining viable counts for two of the reference strains, and the viable counts varied markedly: 1.2 h for S. aureus and 6.0 h for S. epidermidis. The determinations of the PAE, the postantibiotic sub-MIC effect (PA SME), and the sub-MIC effect (SME) for all strains were done with BioScreen C, a computerized incubator for bacteria. The PA SMEs were longer than the SMEs for all strains tested. A newly developed in vitro kinetic model was used to expose the bacteria to continuously decreasing concentrations of vancomycin. A filter prevented the loss of bacteria during the experiments. One reference strain each of S. aureus and S. epidermidis and two clinical isolates of S. aureus were exposed to an initial concentration of 10× the MIC of vancomycin with two different half-lives (t1/2s): 1 or 5 h. The post-MIC effect (PME) was calculated as the difference in time for the bacteria to grow 1 log10 CFU/ml from the numbers of CFU obtained at the time when the MIC was reached and the corresponding time for an unexposed control culture. The difference in PME between the strains was not as pronounced as that for the PAE. Furthermore...

‣ Susceptibilities of several drug-resistant herpes simplex virus type 1 strains to alternative antiviral compounds.

Andrei, G; Snoeck, R; De Clercq, E
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /07/1995 Português
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Resistant herpes simplex virus type 1 strains were obtained under the selective pressure of acyclovir, ganciclovir, bromovinyldeoxyuridine, foscarnet, 2-phosphonylmethoxyehtyl (PME) derivatives of adenine and 2,6-diaminopurine, 3-hydroxy-2-phosphonylmethoxypropyl derivatives of adenine and cytosine, and 2-amino-7-(1,3-dihydroxy-2-propoxymethyl)purine (S2242). The drug susceptibility profiles of resistant strains point to differences in the modes of action of PME and 3-hydroxy-2-phosphonylmethoxypropyl derivatives and common mechanisms of action of foscarnet, S2242, and PME derivatives against herpes simplex virus type 1 replication.

‣ The reactivity of phosphomono-and phosphodiester groups in oligonucleotides.

Knorre, D G; Zrytova, V F; Lebedev, A V; Khalimskaya, L M; Sheshogova, E A
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /04/1978 Português
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The rate constants were estimated by phosphorus NMR spectroscopy for the reactions of alcohols (Tr-dT, 2-cyanoethanol) in pyridine with the main types of the reactive phosphorylating intermediates formed by treatment of pdT-Ac, pdTpdT-Ac, Tr-dTpdT-Ac, Tr-dTpdTpdT-Ac with 2, 4, 6-triisopropylbenzene-sulfonyl chloride (TPS): 1) B type derivatives with phosphomono ester (PME) group converted to a phosphoryl pyridinium residue; 2) C type derivatives with PME and phosphodiester (PDE) groups converted to trisubstituted pyrophosphate; 3) D type derivatives with PDE groups converted to tetrasubstituted pyrophosphate. The two latter types are partially present as cyclic intramolecular pyrophosphates Ci and Di. The reactivity of the intermediates decrease in the series B greater than Ci approximately Di greater than C approximately D. The Ci derivative of pdTpdT-Ac when obtained in dimethylformamide was found to be rather stable to hydrolysis and could be separated from the other dinucleotide derivatives by ion-exchange chromatography. The Arrhenius parameters of all steps of the conversion of PME group of pdT-Ac to B derivative and of the reaction of TPS with PDE group of dinucleoside phosphate Tr-dTpdT-Ac were measured.

‣ Color-blind fluorescence detection for four-color DNA sequencing

Lewis, Ernest K.; Haaland, Wade C.; Nguyen, Freddy; Heller, Daniel A.; Allen, Matthew J.; MacGregor, Robert R.; Berger, C. Scott; Willingham, Britain; Burns, Lori A.; Scott, Graham B. I.; Kittrell, Carter; Johnson, Bruce R.; Curl, Robert F.; Metzker, Mich
Fonte: National Academy of Sciences Publicador: National Academy of Sciences
Tipo: Artigo de Revista Científica
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We present an approach called pulsed multiline excitation (PME) for measurements of multicomponent, fluorescence species and demonstrate its application in capillary electrophoresis for DNA sequencing. To fully demonstrate the advantages of PME, a fluorescent dye set has been developed whose absorption maxima span virtually the entire visible spectrum. Unlike emission wavelength-dependent approaches for identifying fluorescent species, the removal of the spectral component in PME confers a number of advantages including higher and normalized signals from all dyes present in the assay, the elimination of spectral cross-talk between dyes, and higher signal collection efficiency. Base-calling is unambiguously determined once dye mobility corrections are made. These advantages translate into significantly enhanced signal quality as illustrated in the primary DNA sequencing data and provide a means for achieving accurate base-calling at lower reagent concentrations.

‣ Postharvest Variation in Cell Wall-Degrading Enzymes of Papaya (Carica papaya L.) during Fruit Ripening 1

Paull, Robert E.; Chen, Nancy Jung
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /06/1983 Português
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Pectin methylesterase (PME), polygalacturonase (PG), xylanase, cellulase, and proteinase activity were determined and related to respiration, ethylene evolution, and changes in skin color of papaya (Carica papaya L.) fruit from harvest through to the start of fruit breakdown. PME gradually increased from the start of the climacteric rise reaching a peak 2 days after the respiratory peak. PG and xylanase were not detectable in the preclimacteric stage but increased during the climacteric: during the post climacteric stage, the PG declined to a level one-quarter of peak activity with xylanase activity returning to zero. Cellulase activity gradually increased 3-fold after harvest to peak at the same time as PME, 2 days after the edible stage. Proteinase declined throughout the climacteric and postclimacteric phases. A close relationship exists between PG and xylanase and the rise in respiration, ethylene evolution, and softening. Cultivar differences in postclimacteric levels of enzymic activity were not detected.

‣ Tandem mass spectrometric analysis of aspergillus niger pectin methylesterase: mode of action on fully methyl-esterified oligogalacturonates.

Kester, H C; Benen, J A; Visser, J; Warren, M E; Orlando, R; Bergmann, C; Magaud, D; Anker, D; Doutheau, A
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 01/03/2000 Português
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The substrate specificity and the mode of action of Aspergillus niger pectin methylesterase (PME) was determined using both fully methyl-esterified oligogalacturonates with degrees of polymerization (DP) 2-6 and chemically synthesized monomethyl trigalacturonates. The enzymic activity on the different substrates and a preliminary characterization of the reaction products were performed by using high-performance anion-exchange chromatography at neutral pH. Electrospray ionization tandem MS (ESI-MS/MS) was used to localize the methyl esters on the (18)O-labelled reaction products during the course of the enzymic reaction. A. niger PME is able to hydrolyse the methyl esters of fully methyl-esterified oligogalacturonates with DP 2, and preferentially hydrolyses the methyl esters located on the internal galacturonate residues, followed by hydrolysis of the methyl esters towards the reducing end. This PME is unable to hydrolyse the methyl ester of the galacturonate moiety at the non-reducing end.

‣ Molecular Dynamics Simulations of Lipid Bilayers: Major Artifacts Due to Truncating Electrostatic Interactions

Patra, M.; Karttunen, M.; Hyvönen, M. T.; Falck, E.; Lindqvist, P.; Vattulainen, I.
Fonte: Biophysical Society Publicador: Biophysical Society
Tipo: Artigo de Revista Científica
Publicado em /06/2003 Português
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We study the influence of truncating the electrostatic interactions in a fully hydrated pure dipalmitoylphosphatidylcholine (DPPC) bilayer through 20 ns molecular dynamics simulations. The computations in which the electrostatic interactions were truncated are compared to similar simulations using the particle-mesh Ewald (PME) technique. All examined truncation distances (1.8–2.5 nm) lead to major effects on the bilayer properties, such as enhanced order of acyl chains together with decreased areas per lipid. The results obtained using PME, on the other hand, are consistent with experiments. These artifacts are interpreted in terms of radial distribution functions g(r) of molecules and molecular groups in the bilayer plane. Pronounced maxima or minima in g(r) appear exactly at the cutoff distance indicating that the truncation gives rise to artificial ordering between the polar phosphatidyl and choline groups of the DPPC molecules. In systems described using PME, such artificial ordering is not present.

‣ Pharmacodynamic Model To Describe the Concentration-Dependent Selection of Cefotaxime-Resistant Escherichia coli

Olofsson, Sara K.; Geli, Patricia; Andersson, Dan I.; Cars, Otto
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /12/2005 Português
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Antibiotic dosing regimens may vary in their capacity to select mutants. Our hypothesis was that selection of a more resistant bacterial subpopulation would increase with the time within a selective window (SW), i.e., when drug concentrations fall between the MICs of two strains. An in vitro kinetic model was used to study the selection of two Escherichia coli strains with different susceptibilities to cefotaxime. The bacterial mixtures were exposed to cefotaxime for 24 h and SWs of 1, 2, 4, 8, and 12 h. A mathematical model was developed that described the selection of preexisting and newborn mutants and the post-MIC effect (PME) as functions of pharmacokinetic parameters. Our main conclusions were as follows: (i) the selection between preexisting mutants increased with the time within the SW; (ii) the emergence and selection of newborn mutants increased with the time within the SW (with a short time, only 4% of the preexisting mutants were replaced by newborn mutants, compared to the longest times, where 100% were replaced); and (iii) PME increased with the area under the concentration-time curve (AUC) and was slightly more pronounced with a long elimination half-life (T1/2) than with a short T1/2 situation, when AUC is fixed. We showed that...

‣ Separation of Arabidopsis Pollen Tetrads Is Regulated by QUARTET1, a Pectin Methylesterase Gene1[W]

Francis, Kirk E.; Lam, Sandy Y.; Copenhaver, Gregory P.
Fonte: American Society of Plant Biologists Publicador: American Society of Plant Biologists
Tipo: Artigo de Revista Científica
Publicado em /11/2006 Português
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Arabidopsis (Arabidopsis thaliana) QUARTET (QRT) genes are required for pollen separation during normal floral development. In qrt mutants, the four products of microsporogenesis remain fused and pollen grains are released as tetrads. In Arabidopsis, tetrad analysis in qrt mutants has been used to map all five centromeres, easily distinguish sporophytic from gametophytic mutations, and accurately assess crossover interference. Using a combination of forward and reverse genetics, we have identified the gene responsible for the qrt1 phenotype. Annotation predicts that QRT1 encodes a pectin methylesterase (PME), and enzymatic assays of QRT1 expressed in Escherichia coli indicate that QRT1 has PME activity. Promoter and transcription analysis demonstrate QRT1 is expressed in anther tissues shortly after meiosis is complete. Unexpectedly, the QRT1 promoter is also active in a variety of developmentally unrelated tissues, including developing guard cells, the hypocotyl-root transition zone, areas of lateral root emergence, and floral nectaries. PMEs constitute a large gene family in Arabidopsis, are involved in cell wall loosening, and have been implicated in various aspects of floral development and pollen tube elongation. The identification of QRT1 as a PME contributes to our understanding of pollen development and may help to provide valuable genetic tools in other plant species.

‣ Pectin Methyl Esterase Inhibits Intrusive and Symplastic Cell Growth in Developing Wood Cells of Populus12[W][OA]

Siedlecka, Anna; Wiklund, Susanne; Péronne, Marie-Amélie; Micheli, Fabienne; Leśniewska, Joanna; Sethson, Ingmar; Edlund, Ulf; Richard, Luc; Sundberg, Björn; Mellerowicz, Ewa J.
Fonte: American Society of Plant Biologists Publicador: American Society of Plant Biologists
Tipo: Artigo de Revista Científica
Publicado em /02/2008 Português
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Wood cells, unlike most other cells in plants, grow by a unique combination of intrusive and symplastic growth. Fibers grow in diameter by diffuse symplastic growth, but they elongate solely by intrusive apical growth penetrating the pectin-rich middle lamella that cements neighboring cells together. In contrast, vessel elements grow in diameter by a combination of intrusive and symplastic growth. We demonstrate that an abundant pectin methyl esterase (PME; EC 3.1.1.11) from wood-forming tissues of hybrid aspen (Populus tremula × tremuloides) acts as a negative regulator of both symplastic and intrusive growth of developing wood cells. When PttPME1 expression was up- and down-regulated in transgenic aspen trees, the PME activity in wood-forming tissues was correspondingly altered. PME removes methyl ester groups from homogalacturonan (HG) and transgenic trees had modified HG methylesterification patterns, as demonstrated by two-dimensional nuclear magnetic resonance and immunostaining using PAM1 and LM7 antibodies. In situ distributions of PAM1 and LM7 epitopes revealed changes in pectin methylesterification in transgenic trees that were specifically localized in expanding wood cells. The results show that en block deesterification of HG by PttPME1 inhibits both symplastic growth and intrusive growth. PttPME1 is therefore involved in mechanisms determining fiber width and length in the wood of aspen trees.

‣ Cell Wall Polysaccharides Are Specifically Involved in the Exclusion of Aluminum from the Rice Root Apex1

Yang, Jian Li; Li, Ya Ying; Zhang, Yue Jiao; Zhang, Shan Shan; Wu, Yun Rong; Wu, Ping; Zheng, Shao Jian
Fonte: American Society of Plant Biologists Publicador: American Society of Plant Biologists
Tipo: Artigo de Revista Científica
Publicado em /02/2008 Português
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Rice (Oryza sativa) is the most aluminum (Al)-resistant crop species among the small-grain cereals, but the mechanisms responsible for this trait are still unclear. Using two rice cultivars differing in Al resistance, rice sp. japonica ‘Nipponbare’ (an Al-resistant cultivar) and rice sp. indica ‘Zhefu802’ (an Al-sensitive cultivar), it was found that Al content in the root apex (0–10 mm) was significantly lower in Al-resistant ‘Nipponbare’ than in sensitive ‘Zhefu802’, with more of the Al localized to cell walls in ‘Zhefu802’, indicating that an Al exclusion mechanism is operating in ‘Nipponbare’. However, neither organic acid efflux nor changes in rhizosphere pH appear to be responsible for the Al exclusion. Interestingly, cell wall polysaccharides (pectin, hemicellulose 1, and hemicellulose 2) in the root apex were found to be significantly higher in ‘Zhefu802’ than in ‘Nipponbare’ in the absence of Al, and Al exposure increased root apex hemicellulose content more significantly in ‘Zhefu802’. Root tip cell wall pectin methylesterase (PME) activity was constitutively higher in ‘Zhefu802’ than in ‘Nipponbare’, although Al treatment resulted in increased PME activity in both cultivars. Immunolocalization of pectins showed a higher proportion of demethylated pectins in ‘Zhefu802’...

‣ A Homozygous Mutation in Human PRICKLE1 Causes an Autosomal-Recessive Progressive Myoclonus Epilepsy-Ataxia Syndrome

Bassuk, Alexander G.; Wallace, Robyn H.; Buhr, Aimee; Buller, Andrew R.; Afawi, Zaid; Shimojo, Masahito; Miyata, Shingo; Chen, Shan; Gonzalez-Alegre, Pedro; Griesbach, Hilary L.; Wu, Shu; Nashelsky, Marcus; Vladar, Eszter K.; Antic, Dragana; Ferguson, Pol
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em 17/11/2008 Português
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Progressive myoclonus epilepsy (PME) is a syndrome characterized by myoclonic seizures (lightning-like jerks), generalized convulsive seizures, and varying degrees of neurological decline, especially ataxia and dementia. Previously, we characterized three pedigrees of individuals with PME and ataxia, where either clinical features or linkage mapping excluded known PME loci. This report identifies a mutation in PRICKLE1 (also known as RILP for REST/NRSF interacting LIM domain protein) in all three of these pedigrees. The identified PRICKLE1 mutation blocks the PRICKLE1 and REST interaction in vitro and disrupts the normal function of PRICKLE1 in an in vivo zebrafish overexpression system. PRICKLE1 is expressed in brain regions implicated in epilepsy and ataxia in mice and humans, and, to our knowledge, is the first molecule in the noncanonical WNT signaling pathway to be directly implicated in human epilepsy.

‣ Comparison of the Extended Isotropic Periodic Sum and Particle Mesh Ewald Methods for Simulations of Lipid Bilayers and Monolayers

Venable, Richard M.; Chen, Linda E.; Pastor, Richard W.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 30/04/2009 Português
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3D-IPS/DFFT is an extension of the three dimensional Isotropic Periodic Sum (3D-IPS) for evaluation of electrostatic and Lennard-Jones interactions in heterogeneous systems; it utilizes a discrete fast Fourier transform (DFFT) for efficient calculation of the IPS potential with a large local region radius. The method is demonstrated to be highly accurate for simple bulk fluids, liquid/liquid and liquid/vapor interfaces, and lipid bilayers and monolayers. Values for rC (the cutoff distance for direct evaluation of pairs) and RC (the local region radius) equal to 10 Å and twice the longest edge of the periodic cell, respectively, provide excellent efficiency and accuracy. Dimyristoylphosphatidylcholine (DMPC) monolayers simulated with the CHARMM (Chemistry at HARvard Molecular Mechanics) C27r lipid parameter set and 3D-IPS/DFFT yield surface tensions approximately 8 dyn/cm higher than those simulated using Particle Mesh Ewald (PME), and with experiment. In contrast, surface tensions for DMPC bilayers are 16 dyn/cm/leaflet with both 3D-IPS/DFFT (rC = 10 and 12 Å) and PME (rC = 12 Å). This indicates that PME (rC = 12 Å) may be used for simulations of bilayers, but not monolayers, and that the large bilayer surface tension arising from C27r is incorrect.

‣ Impact of Sacral Surface Therapeutic Electrical Stimulation on Early Recovery of Urinary Continence after Radical Retropubic Prostatectomy: A Pilot Study

Nakagawa, Haruo; Kaiho, Yasuhiro; Namiki, Shunichi; Ishidoya, Shigeto; Saito, Seiichi; Arai, Yoichi
Fonte: Hindawi Publishing Corporation Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
Português
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Objectives. To investigate whether sacral surface therapeutic electrical stimulation (SSTES) initiated during the early postoperative period would be effective towards early recovery of postprostatectomy urinary continence. Methods. A total of 35 consecutive patients who underwent radical prostatectomy by a single surgeon were enrolled in this study. Twenty early patients began pelvic floor muscle exercise (PME). Fifteen subsequent patients received SSTES postoperatively with no instruction for PME provided. Immediate urinary function just after catheter removal was evaluated with frequency-volume chart and 24-hour pad test. Results. There were no differences between the SSTES and PME groups in maximum voided volume capacity (MVV) and urine loss ratio (ULR) on the first day after removal of urethral catheter. However, on day 3 MVV was significantly larger and ULR was also significantly lower in the SSTES group. Conclusions. SSTES treatment is feasible and appears to be effective for early recovery of urinary continence after radical prostatectomy.

‣ The Human Gonadotropin Releasing Hormone Type I Receptor Is a Functional Intracellular GPCR Expressed on the Nuclear Membrane

Re, Michelle; Pampillo, Macarena; Savard, Martin; Dubuc, Céléna; McArdle, Craig A.; Millar, Robert P.; Conn, P. Michael; Gobeil, Fernand; Bhattacharya, Moshmi; Babwah, Andy V.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 08/07/2010 Português
Relevância na Pesquisa
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The mammalian type I gonadotropin releasing hormone receptor (GnRH-R) is a structurally unique G protein-coupled receptor (GPCR) that lacks cytoplasmic tail sequences and displays inefficient plasma membrane expression (PME). Compared to its murine counterparts, the primate type I receptor is inefficiently folded and retained in the endoplasmic reticulum (ER) leading to a further reduction in PME. The decrease in PME and concomitant increase in intracellular localization of the mammalian GnRH-RI led us to characterize the spatial distribution of the human and mouse GnRH receptors in two human cell lines, HEK 293 and HTR-8/SVneo. In both human cell lines we found the receptors were expressed in the cytoplasm and were associated with the ER and nuclear membrane. A molecular analysis of the receptor protein sequence led us to identify a putative monopartite nuclear localization sequence (NLS) in the first intracellular loop of GnRH-RI. Surprisingly, however, neither the deletion of the NLS nor the addition of the Xenopus GnRH-R cytoplasmic tail sequences to the human receptor altered its spatial distribution. Finally, we demonstrate that GnRH treatment of nuclei isolated from HEK 293 cells expressing exogenous GnRH-RI triggers a significant increase in the acetylation and phosphorylation of histone H3...