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‣ Sintomas somáticos de depressão em pacientes portadores de retocolite ulcerativa idiopática; Somatic symptoms of depression in patients with idiopathic ulcerative colitis; Síntomas somáticos de depresión en pacientes con rectocolitis ulcerosa idiopática

SERAFIM, Taís de Souza; COSTA, Ana Lucia Siqueira
Fonte: Escola Paulista de Enfermagem, Universidade Federal de São Paulo Publicador: Escola Paulista de Enfermagem, Universidade Federal de São Paulo
Tipo: Artigo de Revista Científica
Português
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OBJETIVO: Identificar os sintomas depressivos mais freqüentes entre portadores da doença Retocolite Ulcerativa Idiopática (RCUI) e compará-los a um grupo de controle. MÉTODO: A amostra compôs-se de 100 indivíduos adultos portadores de RCUI que freqüentavam o Ambulatório de Doenças Inflamatórias do Cólon do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo que foram comparados a 100 indivíduos isentos da doença. Após os procedimentos éticos devidos todos responderam ao Inventário de Depressão de Beck. RESULTADOS: Dos 21 sintomas depressivos, seis mostraram valores estatísticos significativos para: distorção da imagem corporal, inibição para o trabalho, fadiga, perda do apetite, preocupação somática e diminuição de libido. CONCLUSÃO: Verificou-se que os doentes apresentaram com mais freqüência sintomas depressivos da esfera somática quando comparados ao grupo de controle. A similaridade dos sintomas entre a doença e os sintomas depressivos faz com que os doentes sejam freqüentemente subdiagnosticados.; OBJECTIVE: To identify the most common symptoms of depression in patients with idiopathic Ulcerative Colitis (UC) and to compare these symptoms of depression with those in patients without UC. METHOD: The sample consisted of 100 adults with Ulcerative Colitis attending the outpatient clinic for Inflammatory Bowel Diseases of the "Hospital das Clinicas" of the School of Medicine of the University of São Paulo...

‣ Emprego de terapia celular em modelo experimental de doença inflamatória intestinal.; Employment of cell therapy in experimental model of inflammatory bowel disease.

Marcelino, Monica Yonashiro
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 11/12/2012 Português
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Pretendeu-se, no presente estudo, verificar a segurança e a eficácia do transplante de células-tronco derivadas do tecido adiposo (ASC) em ratos com UC induzida por ácido trinitrobenzenosulfonico (TNBS). A população celular foi isolada do tecido adiposo por dissociação mecânica e cultivada. O comportamento celular foi verificado por meio da morfologia, proliferação, viabilidade, capacidade de aderência à superfície plástica e parâmetros de diferenciação osteogênica, condrogênica e adipogênica. Os animais foram avaliados, considerando-se os aspectos clínicos, macroscópicos, microscópicos e bioquímicos. No modelo experimental de UC, a infusão de ASC reduziu significativamente a presença de aderências entre o cólon e órgãos adjacentes, bem como diminuiu a quantidade de células inflamatórias na mucosa lesada. Conclui-se que as ASC podem promover e/ou acelerar o processo de regeneração da mucosa intestinal inflamada e assim, serem empregadas como uma opção terapêutica com amplo potencial de aplicabilidade no tratamento da DII.; It was intended in this study verify the safety and efficacy of the transplantation of adipose derived stem cells (ASC) in rats with ulcerative colitis induced by trinitrobenzenosulfonico acid (TNBS). The cell population was isolated from the adipose tissue by mechanical dissociation and cultured. The cell behavior was verified by the morphology...

‣ Reativação da retocolite ulcerativa com o uso de droga antiinflamatória não esteróide. Relato de caso e revisão da literatura.

Dichi, I.; Dichi, J. B. ; Frenhane, P. ; Rodrigues, M. A. ; Burini, R. C. ; Victória, C. R.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Revisão Formato: 172-177
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The case of a patient with ulcerative colitis and isolated sacro-ileitis is presented. She suffered reactivation of the intestinal disease with diclofenac. The patient was allergic to sulfasalazine and was using fish oil fatty acid. The possible mechanisms of reactivation of the inflammatory bowel disease with non-steroidal anti-inflammatory drugs are discussed. It is suggested when necessary the utilization of non-steroidal anti-inflammatory drugs that inhibits the lipoxygenase in these patients.

‣ The surgical treatment of patients with ulcerative colitis from an university hospital at Natal, Brazil

Alencar,Suelene Suassuna Silvestre de; Corrêa,Romualdo da Silva; Bezerra,Cátia de França; Menezes,Emanuela Simone Cunha de; Nascimento,Antonio Luiz do; Costa,Davi Aragão Alves da; Alencar,Marcelo José Carlos
Fonte: Sociedade Brasileira de Coloproctologia Publicador: Sociedade Brasileira de Coloproctologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/09/2012 Português
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INTRODUCTION: Ulcerative colitis (UC) is a chronic inflammatory disease that affects the rectum and colon, involving periods of exacerbation and remission. A considerable number of patients requires surgery during the course of this disease. OBJECTIVE: The purpose of this study is to analyze the profile and therapeutic approach of patients with UC. METHODS: This is a retrospective study that analyzed medical records of patients diagnosed with UC between 1999 and 2010. We selected 45 patients and analyzed the following variables: age, gender, ethnic group, interval between onset of symptoms and diagnosis, extraintestinal manifestations, extent of colonic involvement, disease complications, pharmacological treatment, indication for surgery, time between diagnosis and indication, surgical procedures, early and late complications and pathological results of surgical specimens. Data were analyzed descriptively and compared with other studies. RESULTS: The clinical profile of the patients was consistent with the literature. Nine patients underwent surgical treatment: seven were submitted to proctocolectomy with anastomosis in the ileo-anal pouch and two were submitted to total colectomy; in addition, eight were submitted to ileostomy. Postoperative complications occurred in 55.5% of patients. CONCLUSIONS: The study confirms data from the literature regarding the profile and therapeutic approach of patients with ulcerative colitis.

‣ Receptor binding sites for substance P, but not substance K or neuromedin K, are expressed in high concentrations by arterioles, venules, and lymph nodules in surgical specimens obtained from patients with ulcerative colitis and Crohn disease.

Mantyh, C R; Gates, T S; Zimmerman, R P; Welton, M L; Passaro, E P; Vigna, S R; Maggio, J E; Kruger, L; Mantyh, P W
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /05/1988 Português
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Several lines of evidence indicate that tachykinin neuropeptides [substance P (SP), substance K (SK), and neuromedin K (NK)] play a role in regulating the inflammatory and immune responses. To test this hypothesis in a human inflammatory disease, quantitative receptor autoradiography was used to examine possible abnormalities in tachykinin binding sites in surgical specimens from patients with inflammatory bowel disease. Surgical specimens of colon were obtained from patients with ulcerative colitis (n = 4) and Crohn disease (n = 4). Normal tissue was obtained from uninvolved areas of extensive resections for carcinoma (n = 6). In all cases, specimens were obtained less than 5 min after removal to minimize influences associated with degradation artifacts and were processed for quantitative receptor autoradiography by using 125I-labeled Bolton-Hunter conjugates of NK, SK, and SP. In the normal colon a low concentration of SP receptor binding sites is expressed by submucosal arterioles and venules and a moderate concentration is expressed by the external circular muscle, whereas SK receptor binding sites are expressed in low concentrations by the external circular and longitudinal muscle. In contrast, specific NK binding sites were not observed in any area of the human colon. In colon tissue obtained from ulcerative colitis and Crohn disease patients...

‣ Direct demonstration of increased expression of Thomsen-Friedenreich (TF) antigen in colonic adenocarcinoma and ulcerative colitis mucin and its concealment in normal mucin.

Campbell, B J; Finnie, I A; Hounsell, E F; Rhodes, J M
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /02/1995 Português
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Increased binding of the lectin peanut agglutinin is a common feature in epithelial malignancy and hyperplasia. This may have considerable functional importance in the intestine by allowing interaction between the epithelium and mitogenic lectins of dietary or microbial origin. Peanut agglutinin binds the disaccharide Thomsen-Friedenreich (TF, T or core 1) blood group antigen, Gal beta (1-3) GalNAc alpha-, but is not totally specific for this site. Consequently, there has been controversy about the presence of this structure in colon cancer; studies with anti-TF monoclonal antibodies have failed to detect it. We have examined the presence of TF antigen in colonic mucus glycoprotein (mucin) using endo-alpha-N-acetylgalactosaminidase (O-Glycanase), which specifically catalyzes the hydrolysis of TF antigen from glycoconjugates. Samples of adenocarcinoma, inflammatory bowel disease (ulcerative colitis), and normal mucin were treated with O-glycanase, the liberated disaccharide was separated from the glycoprotein and analyzed using dual CarboPac PA-100 column high performance anion-exchange chromatography coupled with pulsed amperometric detection. O-Glycanase treatment released increased amounts of TF antigen from both colonic adenocarcinoma (8.0 +/- 3.9 ng/micrograms protein...

‣ Detection of Species-Specific Helicobacter Ribosomal DNA in Intestinal Biopsy Samples from a Population-Based Cohort of Patients with Ulcerative Colitis

Streutker, C. J.; Bernstein, C. N.; Chan, V. L.; Riddell, R. H.; Croitoru, K.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /02/2004 Português
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The inflammatory bowel diseases are considered an abnormal host immune response to an environmental stimulus. Evidence suggests a role for intestinal bacteria in initiating and/or providing an ongoing stimulus for inflammation in inflammatory bowel disease. Helicobacter pylori is the major cause of active chronic gastritis and peptic ulcers in humans and has been linked to gastric carcinoma and lymphoma. Studies in various animal models, particularly mice, have identified enterohepatic Helicobacter species that are capable of causing hepatitis and enterocolitis. We hypothesize that Helicobacter species may have a role in maintaining inflammation in humans with inflammatory bowel disease. In order to investigate this, biopsy specimens were obtained from patients with and without inflammatory bowel disease. DNA was extracted from the tissues and subjected to PCR with primers designed to detect the ribosomal DNA of members of the Helicobacter species. DNA from six biopsy samples from 60 inflammatory bowel disease patients tested positive. This included 5 of 33 ulcerative colitis patients that were positive compared to 0 of 29 age-matched controls (P < 0.04). Sequencing of the bands produced by PCR amplification revealed ≥99% homology with H. pylori. These results indicate that a member of the Helicobacter species may be involved in some cases of ulcerative colitis.

‣ Bronchial disease in ulcerative colitis.

Higenbottam, T; Cochrane, G M; Clark, T J; Turner, D; Millis, R; Seymour, W
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /08/1980 Português
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Ten patients with ulcerative colitis, all of whom were non-smokers, presented with a productive cough. In six, the chest radiography was normal and cough was the only symptom; three of these patients had a minor obstructive ventilatory defect on testing. Four patients complained of exertional dyspnoea and had both an abnormal chest radiograph with bilateral pulmonary shadows and a mixed obstructive and restrictive ventilatory defect. Bronchial epithelial biopsies from four patients (two with and two without pulmonary shadows) revealed basal reserve cell hyperplasia, basement membrane thickening, and submucosal inflammation, changes more usually associated with cigarette smoking. Inhaled beclomethasone diproprionate relieved cough in seven patients. The occurrence of airway epithelial disease in association with ulcerative colitis raises the possibility of a systemic mechanism affecting both bronchial and colonic epithelium. It does not seem likely that sulphasalazine was the cause of the pulmonary syndrome in these subjects.

‣ Large bowel mucosal dysplasia and carcinoma in ulcerative colitis.

Allen, D C; Biggart, J D; Pyper, P C
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /01/1985 Português
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The clinicopathological details of eight cases of ulcerative colitis complicated by carcinoma of the colon are described. There was a total of 14 primary colonic cancers, six of which were not detected before pathological examination of the resection specimens. The reason for this may be related to atypical tumour growth patterns. Three occurred in flat mucosa, one in a mucosal plaque lesion, and another in polypoidal mucosa. The occurrence, distribution, and morphology of mucosal dysplasia were noted in both resection specimens and biopsies taken at varying stages before resection. Tumour was associated with normal and adjacent dysplastic mucosa of varying grades. The extent and grade of dysplasia were not reliable indicators of tumour differentiation or subsequent clinical outcome. Only two cancers were poorly differentiated. In five cases a total of 23 mucosal biopsies were taken, all less than 12 months before resection. Three rectal biopsies were graded positive for dysplasia and three colonic biopsies indefinite for dysplasia. The subsequent resection specimens showed both dysplastic and carcinomatous changes. Three rectal and 14 colonic biopsies were graded negative for dysplasia despite positive findings in the subsequent resection specimens. This anomaly is partly attributed to the patchy nature of dysplasia in colitic mucosa. Two cases illustrate the possibility of dysplasia pursuing a rapidly progressive course. The mucosal changes of ulcerative colitis were assessed using a recently introduced and standardised international classification.

‣ Value of mucin histochemistry in follow up surveillance of patients with long standing ulcerative colitis.

Jass, J R; England, J; Miller, K
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /04/1986 Português
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It has been suggested that diffuse changes in epithelial mucins may help to identify a subgroup of patients with ulcerative colitis who are at increased risk of developing colorectal cancer. Colonoscopic biopsy specimens were taken from 11 colitic patients shortly before colectomies were performed, in which cancer was identified. These patients were matched to colitic patients without cancer for age, sex, and duration of disease. Sections were stained with high iron diamine and alcian blue (HID-AB), mild periodic acid Schiff, and for peanut lectin binding sugar sequences. The sections were assessed blindly and graded semiquantitatively. Most showed ulcerative colitis in remission or mild active disease. There were no differences between the study and control groups. Metaplastic foci were more common in the group who had cancer. These showed increased staining of mucus by mild periodic acid Schiff and increased binding by peanut lectin to the supranuclear (Golgi) zone. Epithelial dysplasia (detected in four of the patients with cancer showed focal intense binding by peanut lectin to cytoplasm, mucus, and glycocalyx. Although dysplasia differed histochemically from normal tissue, special techniques did not facilitate its diagnosis.

‣ Chronic ulcerative colitis complicated by atypical carcinoid tumour.

Dodd, S M
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /08/1986 Português
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A malignant carcinoid tumour of the rectum was found in a patient who had chronic ulcerative colitis. It had a multifocal origin and had not been macroscopically apparent. This association has been recorded in the colon. Hyperplasia of enterochromaffin cells in ulcerative colitis and also dysplasia of these elements as part of a pancellular dysplasia may occur; and this may be a possible explanation for the development of a carcinoid tumour in this condition.

‣ Proctocolectomy and ileostomy for ulcerative colitis: the longer term story.

Phillips, R K; Ritchie, J K; Hawley, P R
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /07/1989 Português
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Elective surgery for ulcerative colitis usually involves the removal of the entire large bowel with either a conventional ileostomy or the formation of an ileoanal pouch anastomosis. Seventy patients undergoing a one stage elective total proctocolectomy and ileostomy between 1976 (the first year an ileoanal pouch was carried out in this hospital) and 1986 have been studied. We have confirmed that proctocolectomy and ileostomy for ulcerative colitis is not the trouble free operation many presume it to be when considering the alternative of an ileoanal pouch.

‣ The endorectal pull-through for the management of ulcerative colitis in children and adults.

Coran, A G; Sarahan, T M; Dent, T L; Fiddian-Green, R; Wesley, J R; Jordan, F T
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /01/1983 Português
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Between June 1977 and November 1981, 26 children and adults with ulcerative colitis have undergone a total colectomy, an endorectal dissection of the rectal mucosa, and an ileoanostomy. A combined abdominoperineal approach was used to perform the operation, and the mucosal-submucosal rectal tube was dissected out intact from the abdominal approach. Every patient survived the operation and showed marked clinical improvement presumably due to resection of the diseased colon. Three patients developed intestinal obstruction that was successfully treated with an enterolysis. A rectal cuff abscess and a retroperitoneal abscess were the only other complications. The postoperative stooling pattern of each patient was obtained through detailed interviews. All the patients were continent during the day and at night one month after surgery. Twenty-two patients had a median stool frequency of seven per 24 hours one month after surgery. At one year, the average number of stools was seven per day. Six patients experienced a stool frequency of seven per 24 hours two years after surgery. The results with this series of patients had encouraged the authors to continue to recommend this approach to children and adults with ulcerative colitis, since it offers an alternative lifestyle that is more attractive to certain patients than the presence of an abdominal stoma.

‣ Rectal lymphoma after colectomy for ulcerative colitis.

Teare, J P; Greenfield, S M; Slater, S
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /01/1992 Português
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Primary colonic lymphoma is an increasingly recognised complication of ulcerative colitis. We report the first known case of rectal lymphoma occurring after colectomy and ileorectal anastomosis in ulcerative colitis.

‣ Functional impairment of natural killer cells in active ulcerative colitis: reversion of the defective natural killer activity by interleukin 2.

Manzano, L; Alvarez-Mon, M; Abreu, L; Antonio Vargas, J; de la Morena, E; Corugedo, F; Duràntez, A
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /02/1992 Português
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We have studied the functional characteristics and clinical importance of the natural killer (NK) cytotoxicity of peripheral blood mononuclear cells (PBMNC) from patients with ulcerative colitis. Normal NK activity was observed in PBMNC from patients with inactive disease, but a pronounced decrease was found in those with active disease. Clinical change from active to inactive disease was associated with enhancement of the depressed NK activity. The impairment of NK cytotoxicity found in patients with active disese could not be ascribed to a deficient number of NK cells as the amounts of HNK-1+, CD16+ (Leu 11), and CD11b (OKM1) cells in PBMNC were within normal ranges. This defective cytotoxic PBMNC activity was normalised by short term (18 hour) incubation with recombinant interleukin 2 (rIL-2). Moreover, long term (5 day) incubation of these effector cells with rIL-2 induced strong cytotoxic activity against NK resistant and NK sensitive target cells in patients with active and inactive disease. We also found that both precursors and effectors of cytotoxic activity promoted by short term and long term incubation with rIL-2 of PBMNC from the patients showed the phenotype of NK cells (CD16+, CD3-). Taken together, these results show that active ulcerative colitis is associated with a defective function of NK cells that is found to be normal in the inactive stage of the disease. The possible pathogenic and therapeutic implications of these findings are discussed.

‣ Prophylactic effects of olsalazine v sulphasalazine during 12 months maintenance treatment of ulcerative colitis. The Danish Olsalazine Study Group.

Kiilerich, S; Ladefoged, K; Rannem, T; Ranløv, P J
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /02/1992 Português
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In a Danish multicentre trial we compared the relapse preventing effects of olsalazine and sulphasalazine in patients with ulcerative colitis over a 12 month treatment period. Two hundred and twenty seven patients (118 men) with at least two previous attacks of ulcerative colitis were randomly allocated according to a prearranged treatment schedule to olsalazine 500 mg bd or sulphasalazine 1 g bd in a double blind, double dummy fashion. One hundred and ninety seven patients completed the trial. The relapse rate after 12 month in the olsalazine group was 46.9% v 42.4% in the sulphasalazine group with a 95% confidence interval for the difference in proportions of -9% to 18%. Seven per cent of the patients were withdrawn from the trial because of adverse drug reactions and these were equally distributed between the two groups.

‣ Comparison of bismuth citrate and 5-aminosalicylic acid enemas in distal ulcerative colitis: a controlled trial.

Pullan, R D; Ganesh, S; Mani, V; Morris, J; Evans, B K; Williams, G T; Rhodes, J
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /05/1993 Português
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An enema that contained a complex of bismuth citrate and polyacrylate was compared with 5-aminosalicylic acid (5-ASA) enemas for treatment of distal ulcerative colitis. The multicentre trial involving 63 patients was randomised and double blind with enemas given over four weeks; clinical, sigmoidoscopic, and histological assessments were made. Improvements were seen in both treatment groups. Clinical remission was seen in 18 of 32 patients treated with 5-ASA and 12 of 31 patients treated with bismuth citrate-carbomer (chi 2 1.94; p = 0.16). Sigmoidoscopic remission occurred in 20 of 32 patients in the 5-ASA group and 15 of 31 patients given bismuth (chi 2 1.27; p = 0.26). Improvement of rectal biopsy histology by at least one grade was seen in 16 of 32 patients in the 5-ASA group and 14 of 31 patients with bismuth (chi 2 0.15; p = 0.70). Analysis of covariance gave no significant difference between groups, although there was a trend favouring 5-ASA. There was no evidence of bismuth accumulation during the trial. Bismuth enemas may offer a new therapeutic option in distal ulcerative colitis.

‣ Gastrointestinal endoscopy biopsy derived proteomic patterns predict indeterminate colitis into ulcerative colitis and Crohn’s colitis

Ballard, Billy Ray; M’Koma, Amosy Ephreim
Fonte: Baishideng Publishing Group Inc Publicador: Baishideng Publishing Group Inc
Tipo: Artigo de Revista Científica
Português
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Patients with indeterminate colitis (IC) are significantly younger at diagnosis with onset of symptoms before the age of 18 years with significant morbidity in the interim. The successful care of IC is based on microscopic visual predict precision of eventual ulcerative colitis (UC) or Crohn’s colitis (CC) which is not offered in 15%-30% of inflammatory bowel disease (IBD) patients even after a combined state-of-the-art classification system of clinical, visual endoscopic, radiologic and histologic examination. These figures have not changed over the past 3 decades despite the introduction of newer diagnostic modalities. The patient outcomes after restorative proctocolectomy and ileal pouch-anal anastomosis may be painstaking if IC turns into CC. Our approach is aiming at developing a single sensitive and absolute accurate diagnostic test tool during the first clinic visit through endoscopic biopsy derived proteomic patterns. Matrix-assisted-laser desorption/ionization mass spectrometry (MS) and/or imaging MS technologies permit a histology-directed cellular test of endoscopy biopsy which identifies phenotype specific proteins, as biomarker that would assist clinicians more accurately delineate IC as being either a UC or CC or a non-IBD condition. These novel studies are underway on larger cohorts and are highly innovative with significances in differentiating a UC from CC in patients with IC and could lend mechanistic insights into IBD pathogenesis.

‣ Tissue nitrosothiol levels in acute ulcerative colitis A step in disease induction?

Roediger, W.; Cummins, A.; Cowled, P.
Fonte: Academic Press Inc Elsevier Science Publicador: Academic Press Inc Elsevier Science
Tipo: Artigo de Revista Científica
Publicado em //2008 Português
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Background and aims: Production of luminal nitric oxide (NO) in the colon is significantly increased in ulcerative colitis (UC) due to bacterial metabolism or activated immune cells. The effect on tissues of prolonged elevation of nitric oxide is unknown. Formation of nitrosothiols has been discounted as these occur under acidic conditions which nevertheless may prevail in acute colitis. The aim was to measure nitrosothiol formation in colonocytes in acute colitis as well as to assess nitrosation of CoA in vitro. Materials and methods: Fresh rectal biopsies obtained at colonoscopies of cases with acute colitis, newly diagnosed or after recurrent attacks, were analysed. Exposure to glutaraldehyde/formaldehyde was avoided and samples stored in the dark at −80 °C. After mechanical homogenization nitrosothiols were measured with the Saville-Griess reaction with and without exposure to mercuric chloride. The reaction with sulphanilamide was measured at 540 nm after azo dye coupling. By calibration curves with GSNO and tissue protein measurement (Bradford reaction) results were expressed as nmoles/mg protein. Purified samples of CoA were obtained for UV spectroscopic analysis after exposure to nitrite or sodium sulphide. Results: Nitrosothiols were not measureable in tissues exposed to formaldehyde. Nitrosothiol levels were 129.3 ± 25.6 (n = 6) in acute UC compared with healthy controls of 53.2 ± 13.8 (n = 6) (p < 0.02). Nitrosothiols of CoA were measureable at acidic pH (2.0) and increased by the presence of sodium sulphide (0.5 mM). Nitroso-CoA formation to a lesser degree occurred at higher pH (6.9) but amplification with sulphide was not clearly identified. Conclusions: Elevated tissue nitrosothiols are found in association with acute colitis. As both CoA and glutathione are known to be depleted in UC...

‣ Emu oil promotes intestinal repair in rat models of enteric inflammation.

Abimosleh, Suzanne Mashtoub
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2013 Português
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Several disorders of the gastrointestinal (GI) tract including ulcerative colitis, chemotherapy-induced mucositis and non-steroidal anti-inflammatory drug (NSAID)- induced enteropathy, are characterised by inflammation, ulceration, mucosal damage and malabsorption. Treatment options are variably effective, highlighting the need to broaden therapeutic approaches, including adjunctive strategies. Emu Oil, derived from subcutaneous and retroperitoneal Emu adipose tissue, is a rich source of fatty acids (FA). Despite limited rigorous scientific studies, topically applied Emu Oil has demonstrated potent anti-inflammatory properties in vivo. Previously, orally administered Emu Oil improved intestinal architecture in a rat model of mucositis, with early indications of enhanced intestinal repair. Accordingly, this thesis investigated the effects of orally administered Emu Oil in rat models of colitis (colonic damage), NSAID-enteropathy (small intestinal [SI] damage) and on the time course of SI repair in chemotherapy-induced mucositis. In the current study, Emu Oil improved colonic tissue damage associated with dextran sulphate sodium-induced colitis in Sprague Dawley rats and facilitated the repair process (Chapter 2). Improvements were indicated histologically by reduced intestinal damage severity scores and enhanced crypt compensatory elongation in the colon. These findings suggested the potential for Emu Oil to augment conventional treatment approaches for colitis. The effectiveness of Emu Oil in the colon provided impetus to further investigate Emu Oil action proximally...