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‣ Polarization memory effect in optical coherence tomography and dental imaging application

Chen, Yueli; Otis, Linda; Zhu, Quing
Fonte: Society of Photo-Optical Instrumentation Engineers (SPIE) Publicador: Society of Photo-Optical Instrumentation Engineers (SPIE)
Tipo: Artigo de Revista Científica
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We report the existence of polarization memory effect (PME) in optical coherence tomography and investigate its potential applications in dental imaging. We performed the study in three steps. First, microsphere scattering phantoms of different sizes were imaged in order to validate experimental results with PME theory. Both linearly and circularly polarized light were used to probe the samples. Second, healthy tooth samples were scanned and polarization memory effect was identified in dentin. In this step, specific verification and signal processing were performed to rule out possible image interpretation by birefringence effect. Third, we evaluated dentin demineralization with PME. Results show polarization memory can be useful to characterize this dynamic mineralization process for early caries detection and rehabilitation.

‣ Airborne Signals from a Wounded Leaf Facilitate Viral Spreading and Induce Antibacterial Resistance in Neighboring Plants

Dorokhov, Yuri L.; Komarova, Tatiana V.; Petrunia, Igor V.; Frolova, Olga Y.; Pozdyshev, Denis V.; Gleba, Yuri Y.
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
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Many plants release airborne volatile compounds in response to wounding due to pathogenic assault. These compounds serve as plant defenses and are involved in plant signaling. Here, we study the effects of pectin methylesterase (PME)-generated methanol release from wounded plants (“emitters”) on the defensive reactions of neighboring “receiver” plants. Plant leaf wounding resulted in the synthesis of PME and a spike in methanol released into the air. Gaseous methanol or vapors from wounded PME-transgenic plants induced resistance to the bacterial pathogen Ralstonia solanacearum in the leaves of non-wounded neighboring “receiver” plants. In experiments with different volatile organic compounds, gaseous methanol was the only airborne factor that could induce antibacterial resistance in neighboring plants. In an effort to understand the mechanisms by which methanol stimulates the antibacterial resistance of “receiver” plants, we constructed forward and reverse suppression subtractive hybridization cDNA libraries from Nicotiana benthamiana plants exposed to methanol. We identified multiple methanol-inducible genes (MIGs), most of which are involved in defense or cell-to-cell trafficking. We then isolated the most affected genes for further analysis: β-1...

‣ Competitive Activity-Based Protein Profiling Identifies Aza-β-Lactams as a Versatile Chemotype for Serine Hydrolase Inhibition

Zuhl, Andrea M.; Mohr, Justin T.; Bachovchin, Daniel A.; Niessen, Sherry; Hsu, Ku-Lung; Berlin, Jacob M.; Dochnahl, Maximilian; López-Alberca, María P.; Fu, Gregory C.; Cravatt, Benjamin F.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
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Serine hydrolases are one of the largest and most diverse enzyme classes in Nature. Most serine hydrolases lack selective inhibitors, which are needed for assigning functions to these enzymes. We recently discovered a set of aza-β-lactams (ABLs) that act as potent and selective inhibitors of the mammalian serine hydrolase protein-phosphatase methylesterase-1 (PME-1). The ABLs inactivate PME-1 by covalent acylation of the enzyme’s serine nucleophile, suggesting that they could offer a general scaffold for serine hydrolase inhibitor discovery. Here, we have tested this hypothesis by screening ABLs more broadly against cell and tissue proteomes by competitive activity-based protein profiling (ABPP), leading to the discovery of lead inhibitors for several serine hydrolases, including the uncharacterized enzyme alpha, beta-hydrolase-10 (ABHD10). ABPP-guided medicinal chemistry yielded a compound ABL303 that potently (IC50 value ~ 30 nM) and selectively inactivated ABHD10 in vitro and in living cells. A comparison of optimized inhibitors for PME-1 and ABHD10 indicates that modest structural changes that alter steric bulk can tailor the ABL to selectively react with distinct, sequence-unrelated serine hydrolases. Our findings, taken together...

‣ Spinal Muscular Atrophy Associated with Progressive Myoclonic Epilepsy Is Caused by Mutations in ASAH1

Zhou, Jie; Tawk, Marcel; Tiziano, Francesco Danilo; Veillet, Julien; Bayes, Monica; Nolent, Flora; Garcia, Virginie; Servidei, Serenella; Bertini, Enrico; Castro-Giner, Francesc; Renda, Yavuz; Carpentier, Stéphane; Andrieu-Abadie, Nathalie; Gut, Ivo; Le
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em 13/07/2012 Português
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Spinal muscular atrophy (SMA) is a clinically and genetically heterogeneous disease characterized by the degeneration of lower motor neurons. The most frequent form is linked to mutations in SMN1. Childhood SMA associated with progressive myoclonic epilepsy (SMA-PME) has been reported as a rare autosomal-recessive condition unlinked to mutations in SMN1. Through linkage analysis, homozygosity mapping, and exome sequencing in three unrelated SMA-PME-affected families, we identified a homozygous missense mutation (c.125C>T [p.Thr42Met]) in exon 2 of ASAH1 in the affected children of two families and the same mutation associated with a deletion of the whole gene in the third family. Expression studies of the c.125C>T mutant cDNA in Farber fibroblasts showed that acid-ceramidase activity was only 32% of that generated by normal cDNA. This reduced activity was able to normalize the ceramide level in Farber cells, raising the question of the pathogenic mechanism underlying the CNS involvement in deficient cells. Morpholino knockdown of the ASAH1 ortholog in zebrafish led to a marked loss of motor-neuron axonal branching, a loss that is associated with increased apoptosis in the spinal cord. Our results reveal a wide phenotypic spectrum associated with ASAH1 mutations. An acid-ceramidase activity below 10% results in Farber disease...

‣ Behavior of Solvent-Exposed Hydrophobic Groove in the Anti-Apoptotic Bcl-XL Protein: Clues for Its Ability to Bind Diverse BH3 Ligands from MD Simulations

Lama, Dilraj; Modi, Vivek; Sankararamakrishnan, Ramasubbu
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 28/02/2013 Português
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Bcl-XL is a member of Bcl-2 family of proteins involved in the regulation of intrinsic pathway of apoptosis. Its overexpression in many human cancers makes it an important target for anti-cancer drugs. Bcl-XL interacts with the BH3 domain of several pro-apoptotic Bcl-2 partners. This helical bundle protein has a pronounced hydrophobic groove which acts as a binding region for the BH3 domains. Eight independent molecular dynamics simulations of the apo/holo forms of Bcl-XL were carried out to investigate the behavior of solvent-exposed hydrophobic groove. The simulations used either a twin-range cut-off or particle mesh Ewald (PME) scheme to treat long-range interactions. Destabilization of the BH3 domain-containing helix H2 was observed in all four twin-range cut-off simulations. Most of the other major helices remained stable. The unwinding of H2 can be related to the ability of Bcl-XL to bind diverse BH3 ligands. The loss of helical character can also be linked to the formation of homo- or hetero-dimers in Bcl-2 proteins. Several experimental studies have suggested that exposure of BH3 domain is a crucial event before they form dimers. Thus unwinding of H2 seems to be functionally very important. The four PME simulations, however...

‣ Substrate Dynamics in Enzyme Action: Rotations of Monosaccharide Subunits in the Binding Groove are Essential for Pectin Methylesterase Processivity

Mercadante, Davide; Melton, Laurence D.; Jameson, Geoffrey B.; Williams, Martin A.K.; De Simone, Alfonso
Fonte: The Biophysical Society Publicador: The Biophysical Society
Tipo: Artigo de Revista Científica
Publicado em 16/04/2013 Português
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The dynamical behavior of biomacromolecules is a fundamental property regulating a large number of biological processes. Protein dynamics have been widely shown to play a role in enzyme catalysis; however, the interplay between substrate dynamics and enzymatic activity is less understood. We report insights into the role of dynamics of substrates in the enzymatic activity of PME from Erwinia chrysanthemi, a processive enzyme that catalyzes the hydrolysis of methylester groups from the galacturonic acid residues of homogalacturonan chains, the major component of pectin. Extensive molecular dynamics simulations of this PME in complex with decameric homogalacturonan chains possessing different degrees and patterns of methylesterification show how the carbohydrate substitution pattern governs the dynamics of the substrate in the enzyme’s binding cleft, such that substrate dynamics represent a key prerequisite for the PME biological activity. The analyses reveal that correlated rotations around glycosidic bonds of monosaccharide subunits at and immediately adjacent to the active site are a necessary step to ensure substrate processing. Moreover, only substrates with the optimal methylesterification pattern attain the correct dynamical behavior to facilitate processive catalysis. This investigation is one of the few reported examples of a process where the dynamics of a substrate are vitally important.

‣ Species sequence differences determine the interaction of GnRH receptor with the cellular quality control system

Cabrera-Wrooman, Alejandro; Janovick, Jo Ann; Conn, P. Michael
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
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Plasma membrane expression (PME) of the human GnRHR (hGnRHR) is regulated by a primate-specific Lys191 which destabilizes a Cys14-Cys200 bridge required by the cellular quality control system (QCS). A 4-amino, non-contiguous “motif” (Leu112, Gln208, Leu300, Asp302) is required for this effect. The hGnRHR sequence, with or without Lys191, decreases PME and inositol phosphate (IP) production when co-expressed with calnexin, a QCS chaperone. WT rat GnRHR, decreases PME and IP production, when co-expressed with calnexin, but to a lesser degree than hGnRH. When the human sequence contains the rat motif, IP production is closer to that of rat GnRHR. When Lys191 is deleted from hGnRHR and co-expressed with calnexin, IP production is similar to the rat sequence. When rat GnRHR containing Lys191 and the human motif is co-expressed with calnexin, IP production is similar to cells expressing the hGnRHR. The motif sequence appears to be a determinant of calnexin recognition.

‣ Metabolic Profiling of the Rat Liver After Chronic Ingestion of Alpha-Naphthylisothiocyanate Using In Vivo and Ex Vivo Magnetic Resonance Spectroscopy

Solanky, Bhavana S.; Sanchez-Canon, Gina J.; Cobbold, Jeremy F. L.; Taylor-Robinson, Simon D.; Bell, Jimmy D.; Scudamore, Cheryl L.; Ross, Eleanor; Holder, Julie C.; So, Po-Wah; Cox, I. Jane
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
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Certain human diseases affecting the biliary tree can be modeled in rats by ingestion of the hepatobiliary toxin alpha-naphthylisothiocyanate (ANIT). Phosphorus magnetic resonance spectroscopy (MRS) allows the noninvasive monitoring of cell dynamics through detection of phosphodiesters (PDE) and phosphomonoesters (PME). Hepatic 31P MRS techniques were therefore used to study the toxic effects of low-dose chronic ANIT ingestion, with a view toward providing biomarkers sensitive to hepatobiliary dysfunction and cholestatic liver injury. Rats were fed an ANIT supplemented diet at three doses (ANIT_0.05%, ANIT_0.04%, and ANIT_0.025%) for 2 weeks. Data from in vivo MRS were compared with results from pair-fed controls (PFCs). Blood and tissue samples were collected at 2 weeks for clinical chemistry, histology, and 1H magic angle spinning MRS. Increases in PDE, relative to total phosphorus (tPh), were detected in both the ANIT_0.05% and ANIT_0.04% groups (0.07 ± 0.01 and 0.08 ± 0.01, respectively) relative to PFC groups (0.03 ± 0.01 and 0.05 ± 0.01, respectively). An increase in PME/tPh was observed in the ANIT_0.05% group only (0.17 ± 0.02) relative to PFC_0.05% (0.12 ± 0.01). Ex vivo 1H MRS findings supported this, wherein measured phosphocholines (PCs) were increased in ANIT_0.05% and ANIT_0.04% groups. Increases in relative total choline (tCho) distinguished the ANIT_0.05% group from the ANIT_0.04% group. Markers of hepatotoxicity such as raised total bilirubin and alkaline phosphatase were found at all ANIT doses. Histological findings included a dose-related increase in both severity of biliary hyperplasia and focal hepatocellular necrosis. Here...

‣ The Effects of Pilates Mat Exercise on the Balance Ability of Elderly Females

Hyun, Ju; Hwangbo, Kak; Lee, Chae-Woo
Fonte: The Society of Physical Therapy Science Publicador: The Society of Physical Therapy Science
Tipo: Artigo de Revista Científica
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[Purpose] The purpose of this study was to examine the effects of Pilates exercise on a mat and balance exercise on an unstable base of support for trunk stability on the balance ability of elderly females. [Subjects and Methods] Forty elderly women aged 65 or older were equally assigned to a Pilates mat exercise (PME) group and an unstable support surface exercise (USSE) group. They conducted exercise three times per week for 12 weeks for 40 minutes each time. In order to examine balance, sway length and the speed of the center of foot pressure were measured for one minute, and in order to examine dynamic balance, the Timed Up and Go (TUG) test was conducted. [Results] After the intervention, sway length, sway speed, and TUG significantly decreased in both groups. A comparison of sway speed after the intervention between the two groups revealed that the PME group showed larger decreases than the USSE group. [Conclusion] PME and USSE elicited significant effects on the static and dynamic balance of elderly female subjects, suggesting that those exercises are effective at enhancing the balance ability of this group of subjects. However, the Pilates mat exercise is regarded as being safer than exercise on an unstable base of support.

‣ PECTOPLATE: the simultaneous phenotyping of pectin methylesterases, pectinases, and oligogalacturonides in plants during biotic stresses

Lionetti, Vincenzo
Fonte: Frontiers Media S.A. Publicador: Frontiers Media S.A.
Tipo: Artigo de Revista Científica
Publicado em 13/05/2015 Português
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Degradation of pectin, a major component of plant cell wall, is important for fungal necrotrophs to achieve a successful infection. The activities of pectin methylesterases (PMEs) from both plants and pathogens and the degree and pattern of pectin methylesterification are critical for the outcome of plant–pathogen interaction. Partial degradation of pectin by pectin degrading enzymes releases oligogalacturonides (OGs), elicitors of plant defense responses. Few analytical techniques are available to monitor pectin methylesterification-modulating machineries and OGs produced during plant pathogen interaction. In the present study, ruthenium red is presented as useful dye to monitor both Botrytis cinerea mycelium growth and the induction of PME activity in plant tissue during fungal infection. Moreover a simple, inexpensive and sensitive method, named PECTOPLATE, is proposed that allows a simultaneous phenotyping of PME and pectinase activities expressed during pathogen infection and of pectinase potential in generating OGs. The results in the manuscript also indicate that PME inhibitors can be used in PECTOPLATE as a tool to discriminate the activities of plant PMEs from those of pathogen PMEs expressed during pathogenesis.

‣ Elemental bioimaging of Cisplatin in Caenorhabditis elegans by LA-ICP-MS

Crone, Barbara; Aschner, Michael; Schwerdtle, Tanja; Karst, Uwe; Bornhorst, Julia
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 08/07/2015 Português
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Cis-diamminedichloroplatinum(II) (Cisplatin) is one of the most important and frequently used cytostatic drugs for the treatment of various solid tumors. Herein, a laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) method incorporating a fast and simple sample preparation protocol was developed for the elemental mapping of Cisplatin in the model organism Caenorhabditis elegans (C. elegans). The method allows imaging of the spatially-resolved elemental distribution of platinum in the whole organism with respect to the anatomic structure in L4 stage worms at a lateral resolution of 5 µm. In addition, a dose- and time-dependent Cisplatin uptake was corroborated quantitatively by a total reflection X-ray fluorescence spectroscopy (TXRF) method, and the elemental mapping indicated that Cisplatin is located in the intestine and in the head of the worms. Better understanding of the distribution of Cisplatin in this well-established model organism will be instrumental in deciphering Cisplatin toxicity and pharmacokinetics. Since the cytostatic effect of Cisplatin is based on binding the DNA by forming intra- and interstrand crosslinks, the response of poly(ADP-ribose)metabolism enzyme 1 (pme-1) deletion mutants to Cisplatin was also examined. Loss of pme-1...

‣ Use of response surface methodology to optimization of extraction of enzymes from pineapple pulp.

COSTA, A. S.; SILVA, C. R. da; COSTA, L. M. S; BARROS, N. A. de M.; KOBLITZ, M. G. B.; VIANA, E. de S.; SOUZA, F. V. D.
Fonte: Pineapple News, n. 17, p. 8, Aug. 2010. Addendum to Newsletter Pineapple Working Group of the I.S.H.S. Publicador: Pineapple News, n. 17, p. 8, Aug. 2010. Addendum to Newsletter Pineapple Working Group of the I.S.H.S.
Tipo: Resumo em anais de congresso (ALICE)
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The enzymatic reactions are very important for food. They are responsíble for the formation of highly desirable cornpound, but can cause disorders and undesirable consequences. The physiological behaviour of vegetables and fruits is already known, so that most of its deterioration changes in flavour, colour and nutritional value are caused by enzymes of the Oxidoreductases group, mainly peroxidases (PER) and polyphenol oxidase (PPO); The degradation of pectic polysaccharides involves two important enzymatic processed whose action is due to pectin methytesterase (PME) and po/ygalacturonase (PG) enzymes which affect the consistency and texture of fruit during ripening and postharvest handling. Pineapp/e is a tropical fruit very appreciated by consumers, but its physical-chemical and biochemical composition varies according to the time and place of production, and maturity stage. In order to optimize the extraction process of enzymes PER, PPO, PME and PG from pineapple pulp (ev. Peróla) the methodology of response surface was used (STATISTICA 7.0) to study the effect of pH (from 4.0 to 8.0) and NaCI concentration (from 0.0 to 2.0 M) of lhe buffer solulion used in lhe extraction of lhe enzymes. The results showed that the best conditions for enzymes extraction were 1.0 M NaCl...

‣ A gest?o pedag?gica do programa mais educa??o: um olhar a partir da experi?ncia da escola Teodora Bentes

SANTOS, ?urea Andrezza Silva dos
Fonte: Universidade Federal do Pará Publicador: Universidade Federal do Pará
Tipo: Dissertação de Mestrado
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Com a redu??o do papel do Estado e a ado??o das pol?ticas neoliberais nas ?reas das pol?ticas sociais como a educa??o, vemos a precariedade da qualidade do ensino p?blico brasileiro. Alguns planos tra?ados nas ?ltimas d?cadas procuram sanar tal problema. O mais recente deles o Plano de Desenvolvimento da Educa??o (PDE) (vigente desde 2007) tem v?rias metas que tem como objetivo a melhoria da qualidade do ensino na educa??o b?sica brasileira. Dentre as v?rias a??es selecionamos o Programa Mais Educa??o (PME), cuja meta ? a educa??o integral, que come?ou a ser implantada nas escolas p?blicas brasileiras a partir de 2008. Tendo como foco o contexto desta pol?tica p?blica este estudo trata da gest?o pedag?gica deste Programa e sua rela??o com os indicadores de aprendizagem: um olhar a partir da experi?ncia da Escola Teodora Bentes entre os anos de 2008 e 2012. A pesquisa tem como caminhos metodol?gicos abordagem qualitativa com o enfoque do materialismo hist?rico-dial?tico, tendo como t?cnicas a an?lise bibliogr?fica e documental, e instrumento a entrevistas semiestruturadas que ser?o realizadas com profissionais da educa??o que atuam na escola como diretor, coordenador do programa, o especialista em educa??o e professores, al?m da an?lise dos indicadores de aprendizagem atrav?s dos boletins...

‣ Italia: la importancia de la pequeña y mediana empresa en el desarrollo industrial

Fonte: CEPAL - Comissão Econômica para a América Latina e o Caribe Publicador: CEPAL - Comissão Econômica para a América Latina e o Caribe
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Incluye Bibliografía; Intenta en forma esquematica: 1) caracterizar la gravitacion de la pequena y mediana empresa (PME) de Italia; 2) explicar el origen del papel desempenado por la PME en ese pais; y 3) destacar algunas modificaciones recientes en el funcionamiento de la PME y su vinculacion con el resto del sistema productivo.

‣ Pulsed-multiline excitation for color-blind fluorescence detection

Scott, Graham B. I.; Kittrell, Carter W.; Curl, Robert F.; Metzker, Michael L.
Fonte: Universidade Rice Publicador: Universidade Rice
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The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population.

‣ Pulsed-multiline excitation for color-blind fluorescence detection

Scott, Graham B. I.; Kittrell, Carter W.; Curl, Robert F.; Metzker, Michael L.
Fonte: Universidade Rice Publicador: Universidade Rice
Português
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The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. This technology differs significantly from the current state-of-the-art DNA sequencing instrumentation, which features single source excitation and color dispersion for DNA sequence identification. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population.

‣ Pulsed-multiline excitation for color-blind fluorescence detection

Scott, Graham B. I.; Kittrell, Carter W.; Curl, Robert F.; Metzker, Michael L.
Fonte: Universidade Rice Publicador: Universidade Rice
Português
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The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population.

‣ La educación emocional a través de las prácticas motrices de expresión. Perspectiva de género

Mateu Serra, Mercè; Romero-Martín, Mª Rosario; Gelpi Fleta, Paula; Rovira Bahillo, Glòria; Lavega Burgués, Pere
Fonte: Universidad de Murcia Publicador: Universidad de Murcia
Tipo: Artigo de Revista Científica Formato: application/pdf
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Esta investigación en Educación Física, analiza el tipo de emociones que suscitan las prácticas de expresión motriz (PME) de carácter cooperativo y psicomotor, según la clasificación de Parlebas. La vivencia emocional se analiza además en base al género y a la sede (localización geográfica) donde se desarrolló el estudio.Se aplicó el cuestionario GES (GamesEmotionalScale) sobre conciencia emocional, a 172 estudiantes (61 mujeres y 111 hombres) de primer curso de Ciencias de la Actividad física y el Deporte y de la diplomatura de Maestro en Educación Física de la Universidad de Barcelona y la Universidad de Zaragoza (sedes de Huesca y Zaragoza). El análisis de los datos mediante ecuaciones de estimación generalizadas mostró que las PME activaron en el alumnado valores elevados de emociones positivas. El do minio de cooperación originó emociones positivas más intensas que el psicomotor. No se encontraron diferencias significativas en la intensidad emocional entre hombres y mujeres, lo que contradice la creencia popular y determinada literatura que atribuye a la mujer una mayor emocionalidad. No se encontraron diferencias significativas entre sedes, aunque sí en alguna interacción al relacionar sedes con tipos de emoción y con tipo de dominio. En definitiva esta investigación confirma el papel destacado de las PME para generar experiencias positivas orientadas hacia la promoción del bienestar del alumnado

‣ Estudio de la fatiga en pacientes con enfermedad de Parkinson : estudio preliminar

Verdes Verdes, Humberto
Fonte: Universidade da Corunha Publicador: Universidade da Corunha
Tipo: Dissertação de Mestrado
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Traballo fin de mestrado (UDC.CIE). Curso 2014/2015; [Resumen] Antecedentes: Una de las quejas más habituales de los enfermos de Parkinson (EP) es la fatiga. La misma tiene un impacto perjudicial muy significativo sobre la calidad de vida de los pacientes. Los mecanismos que subyacen a este síntoma aún no son bien conocidos. Para el estudio de la fatiga se hace necesario establecer unos marcadores neurofisiológicos y observar si su comportamiento difiere en EP y en personas sanas. La comprensión de estos mecanismos podría ayudar a optimizar los tratamientos del síntoma. Objetivos: Revisar las distintas técnicas funcionales objetivas para la evaluación de la fatiga, y su aplicación en EP; e iniciar un protocolo (preliminar) que permita la evaluación de la fatiga en EP, y por tanto establecer marcadores específicos objetivables. Método: Los 8 participantes incluían: 2 EP, 3 jovenes sanos y 3 mayores sanos. Para la evaluación de la fatiga se utilizaron técnicas neurofisiológicas durante la acción del músculo flexor radial del carpo; estas fueron la estimulación magnética transcraneal (EMT) de M1, a través del cual obtenemos la amplitud del potencial motor evocado (PME) y el período de silencio (PS); y la estimulación eléctrica sobre el nervio que permite obtener la onda H y la onda M máxima. La tarea realizada consistía en realizar una contracción voluntaria submáxima (CVSM) isométrica de forma prolongada...

‣ Caracterizaci??n de las enzimas esterasas implicadas en la interacci??n polen-pistilo en el olivo (Olea Europaea L.)

Rej??n Garc??a, Juan David
Fonte: Universidad de Granada Publicador: Universidad de Granada
Tipo: Tese de Doutorado
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El ??xito de la fertilizaci??n en las Angiospermas depende en gran medida de la capacidad del polen para germinar y emitir un tubo pol??nico, el cu??l transporta los n??cleos esperm??ticos a trav??s del pistilo hasta el saco embrionario. En este contexto, los objetivos de este proyecto fueron: 1) determinar la existencia de actividad esterol??tica en el polen de olivo, 2) identificar y clasificar desde el punto de vista funcional las distintas enzimas esterasas, 3) determinar las funciones del exudado estigm??tico y el 'pollen coat', y 4) caracterizar a nivel bioqu??mico y molecular la enzima pectina metilesterasa del polen. El polen de olivo contiene al menos 14 enzimas esterasas, agrupadas en tres clases: carboxilesterasas (2), acetilesterasas (6) y colinesterasas (6). An??lisis funcionales mediante inhibidores qu??micos espec??ficos mostraron un efecto adverso sobre la capacidad de germinar del polen y, en el caso del inhibidor DIFP, una disminuci??n significativa de la longitud del tubo pol??nico. El polen de olivo tambi??n posee al menos 12 enzimas esterasa/lipasa capaces de hidrolizar ??steres de cadena larga. Las distintas enzimas presentaron una localizaci??n celular espec??fica en el grano de polen y en el tubo pol??nico. Los datos obtenidos sugieren que las enzimas esterasas del polen est??n implicadas en la modificaci??n selectiva de la pared celular del tubo pol??nico...