Últimos itens adicionados do Acervo: Biblioteca Digital da Produção Intelectual e Científica da Unicamp

A Biblioteca Digital da Produção Intelectual e Científica da Unicamp tem por objetivo reunir e disseminar a produção intelectual, acadêmica e cultural da universidade e preservar sua memória institucional, além de contribuir para ampliar a visibilidade da instituição e dos seus pesquisadores em âmbito nacional e internacional.

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‣ Inhibitory effects of hydrogen sulfide on lung oxidative stress in allergic mice

Ferreira, H. H. A.; Benetti, L. R.; Campos, D.; Gurgueira, S. A.; Vercesi, A. E.; Teixeira, S. A.; Florenzano, J.; Guedes, C. E. V.; Costa, S. K. P.
Fonte: Wiley-Blackwell; Hoboken Publicador: Wiley-Blackwell; Hoboken
Tipo: Artigo de Revista Científica
Português

‣ In vitro influence of the extracellular matrix in myoepithelial cells stimulated by malignant conditioned medium

Martinez, Elizabeth F.; Dias Demasi, Ana Paula; Napimoga, Marcelo Henrique; Arana-Chavez, Victor Elias; Altemani, Albina; de Araujo, Ney Soares; de Araujo, Vera Cavalcanti
Fonte: Elsevier; Amsterdam Publicador: Elsevier; Amsterdam
Tipo: Artigo de Revista Científica
Português
In order to investigate the role of myoepithelial cell and tumor microenvironment in salivary gland neoplasma, we have performed a study towards the effect of different extracellular matrix proteins (basement membrane matrix, type I collagen and fibronectin) on morphology and differentiation of benign myoepithelial cells from pleomorphic adenoma cultured with malignant cell culture medium from squamous cell carcinoma. We have also analyzed the expression of alpha-smooth muscle actin (alpha-SMA) and FGF-2 by immunofluorescence and qPCR. Our immunofluorescence results, supported by qPCR analysis, demonstrated that alpha-SMA and FGF-2 were upregulated in the benign myoepithelial cells from pleomorphic adenoma in all studied conditions on fibronectin substratum. However, the myoepithelial cells on fibronectin substratum did not alter their morphology under malignant conditioned medium stimulation and exhibited a stellate morphology and, occasionally focal adhesions with the substratum. In summary, our data demonstrated that the extracellular matrix exerts an important role in the morphology of the benign myoepithelial cells by the presence of focal adhesions and also inducing increase FGF-2 and alpha-SMA expression by these cells, especially in the fibronectin substratum. (C) 2011 Elsevier Ltd. All rights reserved.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

‣ Clinical and epidemiological features of blood stream infection caused by Candida "psilosis" complex

Fagnani, R.; Schreiber, A. Z.; Muraosa, Y.; Mikami, Y.; Resende, M. R.; Gonoi, T.; Bonon, S. H. A.; Trabasso, P.; Kamei, K.; Moretti, M. L.
Fonte: Wiley-Blackwell; Hoboken Publicador: Wiley-Blackwell; Hoboken
Tipo: Artigo de Revista Científica
Português

‣ Hb S-Sao Paulo: A new sickling hemoglobin with stable polymers and decreased oxygen affinity

Jorge, Susan E. D. C.; Petruk, Ariel A.; Kimura, Elza M.; Oliveira, Denise M.; Caire, Lucas; Suemasu, Cintia N.; Silveira, Paulo A. A.; Albuquerque, Dulcineia M.; Costa, Fernando F.; Skaf, Munir S.; Martinez, Leandro; Sonati, Maria de Fatima
Fonte: Elsevier; New York Publicador: Elsevier; New York
Tipo: Artigo de Revista Científica
Português
Hb S-Sao Paulo (SP) [HBB:c.20A > T p.Glu6Val: c.196A > G p.Lys65Glu] is a new double-mutant hemoglobin that was found in heterozygosis in an 18-month-old Brazilian male with moderate anemia. It behaves like Hb S in acid electrophoresis, isoelectric focusing and solubility testing but shows different behavior in alkaline electrophoresis, cation-exchange HPLC and RP-HPLC. The variant is slightly unstable, showed reduced oxygen affinity and also appeared to form polymers more stable than the Hb S. Molecular dynamics simulation suggests that the polymerization is favored by interfacial electrostatic interactions. This provides a plausible explanation for some of the reported experimental observations. (C) 2012 Elsevier Inc. All rights reserved.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

‣ Inhibition of Macrophage Oxidative Stress Prevents the Reduction of ABCA-1 Transporter Induced by Advanced Glycated Albumin

Pinto, Raphael de Souza; Castilho, Gabriela; Paim, Bruno Alves; Machado-Lima, Adriana; Inada, Natalia M.; Nakandakare, Edna Regina; Vercesi, Anibal Eugenio; Passarelli, Marisa
Fonte: Springer; Heidelberg Publicador: Springer; Heidelberg
Tipo: Artigo de Revista Científica
Português
We investigated the role of aminoguanidine and benfotiamine on the inhibition of reactive oxygen species (ROS) generation in macrophages induced by advanced glycated albumin (AGE-albumin) and its relationship with cell cholesterol homeostasis, emphasizing the expression of the ATP binding cassette transporter A-1 (ABCA-1). AGE-albumin was made by incubating fatty acid-free albumin with 10 mM glycolaldehyde. ROS production and ABCA-1 protein level were determined by flow cytometry in J774 macrophages treated along time with control (C) or AGE-albumin alone or in the presence of aminoguanidine or benfotiamine. Mitochondrial function was evaluated by oxygraphy. Compared to C-albumin, AGE-albumin increased ROS production in macrophages, which was ascribed to the activities of NADPH oxidase and of the mitochondrial system. Mitochondrial respiratory chain activity was reduced in cells incubated with AGE-albumin. ROS generation along time was associated with the reduction in macrophage ABCA-1 protein level. Aminoguanidine prevented ROS elevation and restored the ABCA-1 content in macrophages; on the other hand, benfotiamine that promoted a lesser reduction in ROS generation was not able to restore ABCA-1 levels. Inhibition of oxidative stress induced by AGE-albumin prevents disturbances in reverse cholesterol transport by curbing the reduction of ABCA-1 elicited by advanced glycation in macrophages and therefore may contribute to the prevention of atherosclerosis in diabetes mellitus.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

‣ Lichen planus and lichenoid drug-induced eruption: a histological and immunohistochemical study

Lage, Denise; Juliano, Priscila Bianchini; Metze, Konradin; de Souza, Elemir Macedo; Cintra, Maria Leticia
Fonte: Wiley-Blackwell; Hoboken Publicador: Wiley-Blackwell; Hoboken
Tipo: Artigo de Revista Científica
Português
Introduction Lichenoid drug eruption (LDE) shares similar features with lichen planus (LP), that could reflect the same pathogenesis. In LP, an autoimmune attack is accepted and cytotoxic T-lymphocytes (CD8+) predominate, especially in late lesions. Apoptosis of keratinocytes may be mediated by CD8+ T and NK cells in two distinct ways: by the release of cytotoxic molecules such as perforin and granzyme B or by the Fas/FasL system. The immunological mechanisms involved in LDE are not yet fully established. Objectives Investigate immunohistological features in LP and LDE to add clues to better understand their pathogenesis. Material and methods Twenty-two patients fulfilled all clinical, laboratory, histopathological, and follow-up features of lichen planus (n = 16) and lichenoid drug eruption (n = 6). Classic histological features favoring LP or LDE were evaluated by two observers. HAM56, MAC387, UCHL-1, OPD4, CD8, Granzyme B, Perforin, and ICAM-1 antibodies were used to decorate the immune infiltrate. Results were analyzed through Pearson correlation, Students t-test, and linear discriminant analysis. Results A higher number of necrotic keratinocytes as well as plasma cells and eosinophils within inflammatory cells were associated with LDE diagnosis. Only in LDE...

‣ Are there relevant differences in the basic geometric structure of the nuclear chromatin between keratoacanthoma and keratoacanthoma-like squamous cell carcinoma?

Metze, K.; Tabai, M.; Adam, R. L.; Watanabe, I.; Magalhaes, R. F.; de Moraes, A. M.; Cintra, M. L.
Fonte: Wiley-Blackwell; Hoboken Publicador: Wiley-Blackwell; Hoboken
Tipo: Artigo de Revista Científica
Português

‣ Influence of Focal and Diffuse Extraprostatic Extension and Positive Surgical Margins on Biochemical Progression Following Radical Prostatectomy

Billis, Athanase; Meirelles, Luciana L.; Freitas, Leandro L. L.; Magna, Luis A.; Reis, Leonardo O.; Ferreira, Ubirajara
Fonte: Brazilian Soc Urol; Rio de Janeiro Publicador: Brazilian Soc Urol; Rio de Janeiro
Tipo: Artigo de Revista Científica
Português
Purpose: The amount of extraprostatic extension and positive surgical margin correlates in most studies with biochemical recurrence following radical prostatectomy. We studied the influence of focal and diffuse extraprostatic extension and positive surgical margins on biochemical progression using a simple method for quantification. Materials and Methods: A total of 360 prostates were step-sectioned and totally processed from 175 patients with stage T1c and 185 patients with clinical stage T2 submitted to radical retropubic prostatectomy. Extraprostatic extension was stratified into 2 groups: present up to 1 quadrant and/or section from the bladder neck or apex (Group 1, focal) and in more than 1 quadrant or section (Group 2, diffuse); and, positive surgical margin present up to 2 quadrants and/or sections (Group 1, focal) and in more than 2 quadrants or sections (Group 2, diffuse). The Kaplan-Meier product-limit analysis was used for the time to biochemical recurrence, and an univariate and multivariate Cox stepwise logistic regression model to identify significant predictors. Results: Extraprostatic extension was found in 129/360 (35.8%) patients, 39/129 (30.2%) in Group 1 and 90/129 (69.8%) in Group 2. In univariate analysis but not in multivariate analysis...

‣ Validation of the Contemporary Epstein Criteria for Insignificant Prostate Cancer in South-American Men

Billis, A.; Meirelles, L.; Freitas, L. L. L. L.; Tavares, A. A.; Carvalho, F. T.; Fontenele, J. P. U.; Cortes, L. G. F.
Fonte: Nature Publishing Group; New York Publicador: Nature Publishing Group; New York
Tipo: Artigo de Revista Científica
Português

‣ Prostate Total Tumor Extent vs. Index Tumor Extent: Which Is Predictive of Biochemical Recurrence Following Radical Prostatectomy?

Billis, A.; Meirelles, L.; Freitas, L. L. L. L.; Polidoro, A. S.; Fernandes, H. A.; Padilha, M. M.; Magna, L. A.; Reis, L. O.; Ferreira, U.
Fonte: Nature Publishing Group; New York Publicador: Nature Publishing Group; New York
Tipo: Artigo de Revista Científica
Português

‣ The Morphology and Immunohistochemical Phenotypic Expression of Focal Prostatic Atrophy

Billis, A.; Meirelles, L.; Freitas, L. L. L. L.; Lins, B. D.; Bonfito, J. F. L.; Costa, L. B. E.; Poletto, P. H.
Fonte: Nature Publishing Group; New York Publicador: Nature Publishing Group; New York
Tipo: Artigo de Revista Científica
Português

‣ Neoplastic Retraction Clefting on Prostate Needle Biopsies: Is It a Prognostic Factor?

Favaro, W. J.; Billis, A.; Cagnon, V. H. A.; Meirelles, L.; Freitas, L. L. L. L.; Lins, B. D.; Bonfito, J. F. L.; Costa, L. B. E.; Poletto, P. H.
Fonte: Nature Publishing Group; New York Publicador: Nature Publishing Group; New York
Tipo: Artigo de Revista Científica
Português

‣ Characterization of Reactive Stroma in Human Prostate Cancer and Role of Prostate Cancer Stem Cells (PCSCs), Growth Factors, Matrix Metalloproteinases (MMPs) and Steroid Hormone Receptors (SHRs) in Its Pathogenesis

Favaro, W. J.; Billis, A.; Meirelles, L.; Freitas, L. L. L. L.; Reis, L. O.; Ferreira, U.
Fonte: Nature Publishing Group; New York Publicador: Nature Publishing Group; New York
Tipo: Artigo de Revista Científica
Português

‣ Validation of the Contemporary Epstein Criteria for Insignificant Prostate Cancer in South-American Men

Billis, A.; Meirelles, L.; Freitas, L. L. L. L.; Tavares, A. A.; Carvalho, F. F.; Fontenele, J. P. U.; Cortes, L. G. F.
Fonte: Nature Publishing Group; New York Publicador: Nature Publishing Group; New York
Tipo: Artigo de Revista Científica
Português

‣ Prostate Total Tumor Extent vs. Index Tumor Extent: Which Is Predictive of Biochemical Recurrence Following Radical Prostatectomy?

Billis, A.; Meirelles, L.; Freitas, L. L. L. L.; Polidoro, A. S.; Fernandes, H. A.; Padilha, M. M.; Magna, L. A.; Reis, L. O.; Ferreira, U.
Fonte: Nature Publishing Group; New York Publicador: Nature Publishing Group; New York
Tipo: Artigo de Revista Científica
Português

‣ The Morphology and Immunohistochemical Phenotypic Expression of Focal Prostatic Atrophy

Billis, A.; Meirelles, L.; Freitas, L. L. L. L.; Lins, B. D.; Bonfito, J. F. L.; Costa, L. B. E.; Poletto, P. H.
Fonte: Nature Publishing Group; New York Publicador: Nature Publishing Group; New York
Tipo: Artigo de Revista Científica
Português

‣ Neoplastic Retraction Clefting on Prostate Needle Biopsies: Is It a Prognostic Factor?

Favaro, W. J.; Billis, A.; Cagnon, V. H. A.; Meirelles, L.; Freitas, L. L. L. L.; Lins, B. D.; Bonfito, J. F. L.; Costa, L. B. E.; Poletto, P. H.
Fonte: Nature Publishing Group; New York Publicador: Nature Publishing Group; New York
Tipo: Artigo de Revista Científica
Português

‣ Characterization of Reactive Stroma in Human Prostate Cancer and Role of Prostate Cancer Stem Cells (PCSCs), Growth Factors, Matrix Metalloproteinases (MMPs) and Steroid Hormone Receptors (SHRs) in Its Pathogenesis

Favaro, W. J.; Billis, A.; Meirelles, L.; Freitas, L. L. L. L.; Reis, L. O.; Ferreira, U.
Fonte: Nature Publishing Group; New York Publicador: Nature Publishing Group; New York
Tipo: Artigo de Revista Científica
Português

‣ Association of postalimentary lipemia with atherosclerotic manifestations

Tentor, J.; Nakamura, R. T.; Gidlund, M.; Barros-Mazon, S.; Harada, L. M.; Zago, V. S.; Oba, J. F.; de Faria, E. C.
Fonte: Assoc Bras Divulg Cientifica; Sao Paulo Publicador: Assoc Bras Divulg Cientifica; Sao Paulo
Tipo: Artigo de Revista Científica
Português
We identified different lipemic and metabolic responses after the ingestion of a standardized meal by healthy adults and related them to atherosclerotic markers. Samples from 60 normolipidemic adults were collected before and after a liquid meal (40 g fat/m(2) body surface) at 0, 2, 4, 6, and 8 h for measurements of lipids, free fatty acids (FFA), insulin, cholesteryl ester transfer protein (CETP), autoantibodies to epitopes of oxidized LDL (oxLDL Ab), lipolytic activities, and apolipoprotein E polymorphism. Mean carotid intima-media thickness (cIMT) was determined by Doppler ultrasound. The volunteers were classified into early (N = 39) and late (N = 31) triacylglycerol (TAG) responders to the test meal. Late responders showed lower HDL cholesterol concentration at fasting and in the TAG peak, lower insulin and higher FFA concentrations compared to early responders. Multivariate regression analyses showed that mean cIMT was associated with gender (male) and age in early responders and by cholesterol levels at the 6th hour in late responders. oxLDL Ab were explained by lipoprotein lipase and negatively by hepatic lipase and oxLDL Ab (fasting period) by CETP (negative) and FFA (positive). This study is the first to identify a postalimentary insulin resistance state...

‣ Improvement of tetracaine antinociceptive effect by inclusion in cyclodextrins

Franco de Lima, Roberta Aline; de Jesus, Marcelo Bispo; Saia Cereda, Cintia Maria; Tofoli, Giovana Radomille; Cabeca, Luis Fernando; Mazzaro, Irineu; Fraceto, Leonardo Fernandes; de Paula, Eneida
Fonte: Informa Healthcare; London Publicador: Informa Healthcare; London
Tipo: Artigo de Revista Científica
Português
Local anesthetics (LA) are among the most important pharmacological compounds used to attenuate or eliminate pain. However, systemic toxicity is still a limitation for LA application, especially for ester-type drugs, such as tetracaine (TTC) that presents poor chemical stability (due to hydrolysis by plasma esterases). Several approaches have been used to improve LA pharmaceutical properties, including the employment of drug-delivery systems. Here we used betacyclodextrin (beta-CD) or hydroxypropyl-beta-cyclodextrin (HP-beta-CD) to develop two new TTC formulations (TTC:beta-CD and TTC:HP-beta-CD). The inclusion complexes formation, in a 1:1 stoichiometry, was confirmed by differential scanning calorimetry, X-ray diffraction, UV-VIS absorption and fluorescence. Nuclear magnetic resonance (DOSY experiments) revealed that TTC association with HP-beta-CD is stronger (K(a) = 1200 mol/L(-1)) than with beta-CD (K(a) = 845 mol/L(-1)). Moreover, nuclear Overhauser effect (NOE) experiments provided information on the topology of the complexes, where TTC aromatic ring is buried inside the CD hydrophobic cavity. In vitro tests with 3T3 fibroblast cells culture revealed that complexation decreased TTC cytotoxicity. In addition, the total analgesic effect of TTC...