Últimos itens adicionados do Acervo: Biblioteca Digital da Produção Intelectual e Científica da Unicamp

A Biblioteca Digital da Produção Intelectual e Científica da Unicamp tem por objetivo reunir e disseminar a produção intelectual, acadêmica e cultural da universidade e preservar sua memória institucional, além de contribuir para ampliar a visibilidade da instituição e dos seus pesquisadores em âmbito nacional e internacional.

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‣ Microbiological quality and safety of minimally processed vegetables marketed in Campinas, SP-Brazil, as assessed by traditional and alternative methods

Maistro, Liliane Correa; Nago Miya, Norma Teruko; Sant'Ana, Anderson S.; Pereira, Jose Luiz
Fonte: Elsevier; Oxford Publicador: Elsevier; Oxford
Tipo: Artigo de Revista Científica
Português
In this study, a total of 172 samples of minimally processed vegetables (MPV) were collected from supermarkets in the city of Campinas, Brazil. The MPV were analyzed using traditional and/or alternative methods for total aerobic mesophilic bacteria, total coliforms, Escherichia coil, coagulase positive staphylococci, Salmonella and Listeria monocytogenes. All the MPV analyzed presented populations of aerobic mesophilic microorganisms and total coliforms were >4 log(10) CFU/g and 1.0-3.4 log(10) CFU/g, respectively. E. coil was enumerated in only 10 samples out of 172 collected, while none of the 172 samples of MPV presented contamination by coagulase positive Staphylococcus (<10(1) CFU/g). Among the four methods used for detection of Salmonella in MPV (Vidas, 1,2 Test, Reveal, and Traditional), when Reveal was used a total of 29 positive samples were reported. For L monocytogenes, the four methods tested (Vidas, Vip, Reveal, and traditional) performed similarly. The presence of Salmonella and L monocytogenes in MPV was confirmed in one (watercress) and two samples (watercress and escarole), respectively. In conclusion, it has been observed that the microbiological quality of MPV commercialized in Campinas is generally satisfactory. Besides...

‣ J/psi production as a function of charged particle multiplicity in pp collisions at root s=7 TeV

Abelev, B.; Adam, J.; Adamova, D.; Adare, A. M.; Aggarwal, M. M.; Rinella, G. Aglieri; Agocs, A. G.; Agostinelli, A.; Salazar, S. Aguilar; Ahammed, Z.; Masoodi, A. Ahmad; Ahmad, N.; Ahn, S. U.; Akindinov, A.; Aleksandrov, D.; Alessandro, B.; Molina, R. Al
Fonte: Elsevier; Amsterdam Publicador: Elsevier; Amsterdam
Tipo: Artigo de Revista Científica
Português
The ALICE Collaboration reports the measurement of the relative J/psi yield as a function of charged particle pseudorapidity density dN(ch)/d eta in pp collisions at root s = 7 TeV at the LHC. J/psi particles are detected for p(t) > 0, in the rapidity interval vertical bar y vertical bar < 0.9 via decay into e(+)e(-), and in the interval 2.5 < y < 4.0 via decay into mu(+)/mu(-) pairs. An approximately linear increase of the J/psi yields normalized to their event average (dN(J/psi)/dy)/(dN(J/psi)/dy) with (dN(ch)/c eta)/(dN(ch)/d eta) is observed in both rapidity ranges, where dN(ch)/d eta is measured within vertical bar eta vertical bar < 1 and p(t) > 0. In the highest multiplicity interval with (dN(ch)/d eta)(bin)) = 24.1, corresponding to four times the minimum bias multiplicity density, an enhancement relative to the minimum bias J/psi yield by a factor of about 5 at 2.5 < y <4 (8 at vertical bar y vertical bar < 0.9) is observed. (C) 2012 CERN. Published by Elsevier B.V. All rights reserved.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

‣ On the implementation of good manufacturing practices in a small processing unity of mozzarella cheese in Brazil

Costa Dias, Maria Angelica; Sant'Ana, Anderson S.; Cruz, Adriano G.; Faria, Jose de Assis F.; Fernandes de Oliveira, Carlos Augusto; Bona, Evandro
Fonte: Elsevier; Oxford Publicador: Elsevier; Oxford
Tipo: Artigo de Revista Científica
Português
This study reports the implementation of GMPs in a mozzarella cheese processing plant. The mozzarella cheese manufacturing unit is located in the Southwestern region of the state of Parana, Brazil, and processes 20,000 L of milk daily. The implementation of GMP took place with the creation of a multi-disciplinary team and it was carried out in four steps: diagnosis, report of the diagnosis and road map, corrective measures and follow-up of GMP implementation. The effectiveness of actions taken and GMP implementation was compared by the total percentages of non-conformities and conformities before and after implementation of GMR Microbiological indicators were also used to assess the implementation of GMP in the mozzarella cheese processing facility. Results showed that the average percentage of conformity after the implementation of GMP was significant increased to 66%, while before it was 32% (p < 0.05). The populations of aerobic microorganisms and total coliforms in equipment were significantly reduced (p < 0.05) after the implementation of GMP, as well as the populations of total coliforms in the hands of food handlers (p < 0.05). In conclusion, GMP implementation changed the overall organization of the cheese processing unity...

‣ Characterization of Staphylococcus aureus isolates in milk and the milking environment from small-scale dairy farms of Sao Paulo, Brazil, using pulsed-field gel electrophoresis

Lee, S. H. I.; Camargo, C. H.; Goncalves, J. L.; Cruz, A. G.; Sartori, B. T.; Machado, M. B.; Oliveira, C. A. F.
Fonte: Elsevier; New York Publicador: Elsevier; New York
Tipo: Artigo de Revista Científica
Português
This research aimed to evaluate the occurrence of Staphylococcus aureus isolates in milk and in the milking environment of 10 small-scale farms (<400 L/d) located in the regions of Franca and Ribeirao Preto, state of Sao Paulo, Brazil. Two-hundred twenty samples of milk were collected from individual cows, along with 120 samples from bulk tank milk, 389 samples from milking equipment and utensils (teat cups, buckets, and sieves), and 120 samples from milkers' hands. Fifty-six Staph. aureus strains were isolated from 849 analyzed samples (6.6%): 12 (5.5%) from milk samples of individual cows, 26 (21.7%) from samples of bulk tank milk, 14 (3.6%) from samples collected from equipment and utensils, and 4 (3.3%) from samples from milkers' hands. Pulsed-field gel electrophoresis typing of the 56 Staph. aureus isolates by SmaI restriction enzyme resulted in 31 profiles (pulsotypes) arranged in 12 major clusters. Results of this study indicate a low incidence, but wide distribution of Staph. aureus strains isolated from raw milk collected from individual cows and surfaces of milkers' hands and milking equipment in the small-scale dairy farms evaluated. However, the high percentage of bulk milk samples found with Staph. aureus is of public health concern because raw...

‣ Occurrence of molds on laminated paperboard for aseptic packaging, selection of the most hydrogen peroxide- and heat-resistant isolates and determination of their thermal death kinetics in sterile distilled water

Delgado, Denise Aparecida; Sant'Ana, Anderson de Souza; de Massaguer, Pilar Rodriguez
Fonte: Springer; New York Publicador: Springer; New York
Tipo: Artigo de Revista Científica
Português
This study aimed at enumerating molds (heat-labile and heat-resistant) on the surface of paperboard material to be filled with tomato pulps through an aseptic system and at determining the most heat-and hydrogen peroxide-resistant strains. A total of 118 samples of laminated paperboard before filling were collected, being 68 before and 50 after the hydrogen peroxide bath. Seven molds, including heat-resistant strains (Penicillium variotii and Talaromyces flavus) with counts ranging between 0.71 and 1.02 CFU/cm(2) were isolated. P. variotii was more resistant to hydrogen peroxide than T. flavus and was inactivated after heating at 85 degrees C/15 min. When exposed to 35 % hydrogen peroxide at 25 degrees C, T. flavus (F5E2) and N. fischeri (control) were less resistant than P. variotti (F1A1). P. citrinum (F7E2) was shown to be as resistant as P. variotti. The D values (the time to cause one logarithmic cycle reduction in a microbial population at a determined temperature) for spores of P. variotii (F1A1) and N. fischeri (control) with 4 months of age at 85 and 90 degrees C were 3.9 and 4.5 min, respectively. Although the contamination of packages was low, the presence of heat-and chemical-resistant molds may be of concern for package sterility and product stability during shelf-life. To our knowledge...

‣ A remarkable new species of flesh-fly mimicking weevil (Coleoptera: Curculionidae: Conoderinae) from Southeastern Brazil

Vanin, Sergio A.; Guerra, Tadeu J.
Fonte: Magnolia Press; Auckland Publicador: Magnolia Press; Auckland
Tipo: Artigo de Revista Científica
Português
Timorus sarcophagoides, new species (type-locality: Brazil, Minas Gerais, Santana do Riacho - Serra do Cipo, 43 degrees 35'W 19 degrees 17'S, 1200-1300m ASL), is described and illustrated. The new species can be distinguished mainly from the other species of the genus by the sexual dimorphism of the male rostrum armed with a hooked tubercle at the base of the dorsal carina, while the tubercle is absent in the female and the corresponding region of the carina is only tumid. The new species has a striking pattern of coloration and behavior that mimics flesh-flies in the family Sarcophagidae. Observations on the natural history of the new species are reported and discussed.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

‣ Characterization of a new beta titanium alloy, Ti-12Mo-3Nb, for biomedical applications

Gabriel, S. B.; Panaino, J. V. P.; Santos, I. D.; Araujo, L. S.; Mei, P. R.; de Almeida, L. H.; Nunes, A.
Fonte: Elsevier; Lausanne Publicador: Elsevier; Lausanne
Tipo: Artigo de Revista Científica
Português
In recent years, different beta titanium alloys have been developed for biomedical applications with a combination of mechanical properties including a low Young's modulus, high strength, fatigue resistance and good ductility with excellent corrosion resistance. From this perspective, a new metastable beta titanium Ti-12Mo-3Nb alloy was developed with the replacement of both vanadium and aluminum from the traditional Ti-6Al-4V alloy. This paper presents the microstructure, mechanical properties and corrosion resistance of the Ti-12Mo-3Nb alloy heat-treated at 950 degrees C for 1 h. The material was characterized by X-ray diffraction and by scanning electron microscopy. Tensile tests were carried out at room temperature. Corrosion tests were performed using Ringer's solution at 25 degrees C. The results showed that this alloy could potentially be used for biomedical purposes due to its good mechanical properties and spontaneous passivation. (c) 2011 Elsevier B.V. All rights reserved.; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

‣ Assessment of cavitated and active non-cavitated caries lesions in 3-to 4-year-old preschool children: a field study

Parisotto, Thais M.; Steiner-Oliveira, Carolina; de Souza-e-Silva, Cintia M.; Peres, Regina C. R.; Rodrigues, Lidiany K. A.; Nobre-Dos-Santos, Marines
Fonte: Wiley-Blackwell; Malden Publicador: Wiley-Blackwell; Malden
Tipo: Artigo de Revista Científica
Português
Background. The prevalence of early childhood caries (ECC) is high in developing countries; thus, sensitive methods for the early diagnosis of ECC are of prime importance to implement the appropriate preventive measures. Aim. To investigate the effects of the addition of early caries lesions (ECL) into WHO threshold caries detection methods on the prevalence of caries in primary teeth and the epidemiological profile of the studied population. Design. In total, 351 3-to 4-year-old preschoolers participated in this cross-sectional study. Clinical exams were conducted by one calibrated examiner using WHO and WHO + ECL criteria. During the exams, a mirror, a ball-ended probe, gauze, and an artificial light were used. The data were analysed by Wilcoxon and Mc-Nemar's tests (a = 0.05). Results. Good intra-examiner Kappa values at tooth /surface levels were obtained for WHO and WHO + ECL criteria (0.93 /0.87 and 0.75 /0.78, respectively). The dmfs scores were significantly higher (P < 0.05) when WHO + ECL criteria were used. ECLs were the predominant caries lesions in the majority of teeth. Conclusions. The results strongly suggest that the WHO + ECL diagnosis method could be used to identify ECL in young children under field conditions, increasing the prevalence and classification of caries activity and providing valuable information for the early establishment of preventive measures.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

‣ Evaluation of Vickers hardness of different types of acrylic denture base resins with and without glass fibre reinforcement

Farina, Ana Paula; Cecchin, Doglas; Soares, Rodrigo Goncalves; Botelho, Andre Luis; Ferreira Koyama Takahashi, Jessica Mie; Mazzetto, Marcelo Oliveira; Mesquita, Marcelo Ferraz
Fonte: Wiley-Blackwell; Hoboken Publicador: Wiley-Blackwell; Hoboken
Tipo: Artigo de Revista Científica
Português
Objective: To evaluate the Vickers hardness of different acrylic resins for denture bases with and without the addition of glass fibres. Background: It has been suggested that different polymerisation methods, as well as the addition of glass fibre (FV) might improve the hardness of acrylic. Materials and methods: Five types of acrylic resin were tested: Vipi Wave (VW), microwave polymerisation; Vipi Flash (VF), auto-polymerisation; Lucitone (LT), QC20 (QC) and Vipi Cril (VC), conventional heat-polymerisation, all with or without glass fibre reinforcement (GFR) and distributed into 10 groups (n = 12). Specimens were then submitted to Vickers hardness testing with a 25-g load for 30 s. All data were submitted to ANOVA and Tukey's HSD test. Results: A significant statistical difference was observed with regard to the polymerisation method and the GFR (p < 0.05). Without the GFR, the acrylic resin VC presented the highest hardness values, and VF and LT presented the lowest. In the presence of GFR, VC resin still presented the highest Vickers hardness values, and VF and QC presented the lowest. Conclusions: The acrylic resin VC and VW presented higher hardness values than VF and QC resins. Moreover, GFR increased the Vickers hardness of resins VW...

‣ The influence of mandibular implant-retained overdentures in masticatory efficiency

Farias Neto, Arcelino; de Farias Pereira, Brunna Moreira; Xitara, Rafael Lopes; Germano, Adriano Rocha; Medeiros Ribeiro, Jaiane Augusta; Mestriner Junior, Wilson; Porto Carreiro, Adriana da Fonte
Fonte: Wiley-Blackwell; Hoboken Publicador: Wiley-Blackwell; Hoboken
Tipo: Artigo de Revista Científica
Português
Objective: To evaluate the masticatory efficiency of patients rehabilitated with conventional dentures (CDs) or implant-retained mandibular overdentures. Background: Despite the evident benefits of implants on mastication as assessed by subjective patient-based outcomes, the extent of implant overdenture treatment effect on food comminution is not well established. Materials and methods: A randomised clinical trial was carried out with 29 completely edentulous patients divided into two groups. The first group was rehabilitated with a mandibular overdenture retained by two splinted implants with bar-clip system, while the second group was rehabilitated with a mandibular CD. Both groups also were rehabilitated with maxillary CDs. Masticatory efficiency and patient satisfaction were assessed 3 months after denture insertion. Masticatory efficiency was evaluated through the colorimetric method with the beads as the artificial test-food. Comparisons for masticatory efficiency and patient satisfaction were performed using Student's t-test (alpha = 0.05). Results: No significant statistical difference was found for masticatory efficiency (p = 0.198). Patient overall satisfaction was significantly higher for the mandibular overdenture (p < 0.001). In addition...

‣ Water sorption of CH3- and CF3-Bis-GMA based resins with additives

Prakki, Anuradha; Cilli, Renato; Vieira, Ian Matos; Dudumas, Kristina; Pereira, Jose Carlos
Fonte: Univ Sao Paulo Fac Odontologia Bauru; Bauru Publicador: Univ Sao Paulo Fac Odontologia Bauru; Bauru
Tipo: Artigo de Revista Científica
Português
Objectives: To evaluate the effect of additives on the water sorption characteristics of Bis-GMA based copolymers and composites containing TEGDMA, CH(3)Bis-GMA or CF(3)Bis-GMA. Material and methods: Fifteen experimental copolymers and corresponding composites were prepared combining Bis-GMA and TEGDMA, CH(3)Bis-GMA or CF(3)Bis-GMA, with aldehyde or diketone (24 and 32 mol%) totaling 30 groups. For composites, barium aluminosilicate glass and pyrogenic silica was added to comonomer mixtures. Photopolymerization was effected by 0.2 wt% each of camphorquinone and N,N-dimethyl-p-toluidine. Specimen densities in dry and water saturated conditions were obtained by Archimedes' method. Water sorption and desorption were evaluated in a desorption-sorption-desorption cycle. Water uptake (%WU), water desorption (%WD), equilibrium solubility (ES; mu g/mm(3)), swelling (f) and volume increase (%V) were calculated using appropriate equations. Results: All resins with additives had increased %WU and ES. TEGDMA-containing systems presented higher %WU, %WD, ES, f and %V values, followed by resins based on CH(3)Bis-GMA and CF(3)Bis-GMA. Conclusions: Aldehyde and diketone led to increases in the water sorption characteristics of experimental resins.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

‣ Genetic polymorphisms modulate the folate metabolism of Brazilian individuals with Down syndrome

Biselli, J. M.; Zampieri, B. L.; Goloni-Bertollo, E. M.; Haddad, R.; Fonseca, M. F. R.; Eberlin, M. N.; Vannucchi, H.; Carvalho, V. M.; Pavarino, E. C.
Fonte: Springer; Dordrecht Publicador: Springer; Dordrecht
Tipo: Artigo de Revista Científica
Português
Individuals with Down syndrome (DS) carry three copies of the Cystathionine beta-synthase (C beta S) gene. The increase in the dosage of this gene results in an altered profile of metabolites involved in the folate pathway, including reduced homocysteine (Hcy), methionine, S-adenosylhomocysteine (SAH) and S-adenosylmethionine (SAM). Furthermore, previous studies in individuals with DS have shown that genetic variants in genes involved in the folate pathway influence the concentrations of this metabolism's products. The purpose of this study is to investigate whether polymorphisms in genes involved in folate metabolism affect the plasma concentrations of Hcy and methylmalonic acid (MMA) along with the concentration of serum folate in individuals with DS. Twelve genetic polymorphisms were investigated in 90 individuals with DS (median age 1.29 years, range 0.07-30.35 years; 49 male and 41 female). Genotyping for the polymorphisms was performed either by polymerase chain reaction (PCR) based techniques or by direct sequencing. Plasma concentrations of Hcy and MMA were measured by liquid chromatography-tandem mass spectrometry as previously described, and serum folate was quantified using a competitive immunoassay. Our results indicate that the MTHFR C677T...

‣ Oxylipidomics of the cow uterus: effects of the peri-ovulatory endocrine milieu of the estrous cycle

Membrive, C. B.; Scolari, S. C.; Cardoso, D.; Martin, I.; Sales, J. N.; Oliveira, M.; D'Alexandri, F. L.; Mesquita, F. S.; de Paula Nogueira, G.; Wheelock, C.; Dahle, S-E; Binelli, M.
Fonte: Wiley-Blackwell; Hoboken Publicador: Wiley-Blackwell; Hoboken
Tipo: Artigo de Revista Científica
Português
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

‣ Differential geometry of grassmannians and the Plucker map

Anan'in, Sasha; Grossi, Carlos H.
Fonte: Versita; Warsaw Publicador: Versita; Warsaw
Tipo: Artigo de Revista Científica
Português
Using the Plucker map between grassmannians, we study basic aspects of classic grassmannian geometries. For 'hyperbolic' grassmannian geometries, we prove some facts (for instance, that the Plucker map is a minimal isometric embedding) that were previously known in the 'elliptic' case.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

‣ Análise do desenvolvimento das práticas urbanas de lazer relacionadas a produção cultural no período nacional-desenvolvimentista à globalização

ALMEIDA, Marco Antonio Bettine de; GUTIERREZ, Gustavo Luis
Fonte: Escola de Educação Física e Esporte da Universidade de São Paulo Publicador: Escola de Educação Física e Esporte da Universidade de São Paulo
Tipo: Artigo de Revista Científica
Português
Apresentar-se-á um cenário geral do desenvolvimento do lazer desde o nacional-desenvolvimentismo com Getúlio Vargas, Juscelino Kubitschek, Regime Militar até a globalização com a redemocratização e ampliação vertiginosa do lazer de consumo. Este panorama geral possibilitará afirmar que o lazer se desenvolveu pelo investimento estatal. Discutiu-se cada um dos momentos históricos contemplando as ações políticas, econômicas e culturais mais significativas, conforme análise bibliográfica. Objetivou-se construir neste trabalho a complexificação sistêmica do lazer no Brasil, concluindo que o lazer se complexificou a partir da racionalização das formas de vida, sistematização dos tempos e desencantamento do mundo. Com a teoria habermasiana fez-se a síntese entre as esferas de influência do lazer (Estado, Mercado e Cultura). Na conclusão apresentou-se a definição do lazer como fruto das relações entre subjetividades intactas no sistema capitalista, que se desenvolveu por meio da complexificação da sociedade, possuindo uma íntima ligação cultural-artística. Expressando as três esferas do Mundo da Vida (cultura, sociedade e personalidade).; A general view of the development of leisure will be presented from the national development with Getúlio Vargas...

‣ Medidas prospectivas de sintomas depressivos entre idosos residentes na comunidade

BATISTONI, Samila Sathler Tavares; NERI, Anita Liberalesso; CUPERTINO, Ana Paula Fabrino Bretas
Fonte: Faculdade de Saúde Pública da Universidade de São Paulo Publicador: Faculdade de Saúde Pública da Universidade de São Paulo
Tipo: Artigo de Revista Científica
Português
OBJETIVO: Identificar fatores sociodemográficos associados a padrões de incidência, remissão e estabilidade de sintomas depressivos entre idosos residentes na comunidade. MÉTODOS: Estudo prospectivo em que foram entrevistados 310 idosos residentes na comunidade, em Juiz de Fora, MG, entre 2002 e 2004. O seguimento (T2) foi realizado 15,7 meses após a primeira entrevista (T1). Os sintomas de depressão foram avaliados pela escala do Center for Epidemiological Studies - Depression. Os idosos foram classificados segundo a evolução dos sintomas de depressão e comparados quanto às variáveis sociodemográficas com o teste de qui-quadrado e Exato de Fisher. RESULTADOS: Não houve diferenças na prevalência de sintomas depressivos entre T1 e T2 (33,8%). Foram identificados quatro grupos segundo a evolução dos sintomas da primeira para a segunda medida: livres de depressão (50,9%); recorrência (19,7%); incidência (15,2%); remissão (14,2%). Ter pontuado para depressão em T1, ser do sexo feminino e possuir baixa escolaridade representaram riscos para a manifestação de sintomas depressivos em T2. CONCLUSÕES: Piores trajetórias de evolução em sintomatologia depressiva (incidência e recorrência) associaram-se ao gênero feminino.; OBJECTIVE: To identify sociodemographic factors associated with patterns of incidence...

‣ A relaxed constant positive linear dependence constraint qualification and applications

Andreani, Roberto; Haeser, Gabriel; Laura Schuverdt, Maria; Silva, Paulo J. S.
Fonte: Springer; New York Publicador: Springer; New York
Tipo: Artigo de Revista Científica
Português
In this work we introduce a relaxed version of the constant positive linear dependence constraint qualification (CPLD) that we call RCPLD. This development is inspired by a recent generalization of the constant rank constraint qualification by Minchenko and Stakhovski that was called RCRCQ. We show that RCPLD is enough to ensure the convergence of an augmented Lagrangian algorithm and that it asserts the validity of an error bound. We also provide proofs and counter-examples that show the relations of RCRCQ and RCPLD with other known constraint qualifications. In particular, RCPLD is strictly weaker than CPLD and RCRCQ, while still stronger than Abadie's constraint qualification. We also verify that the second order necessary optimality condition holds under RCRCQ.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

‣ Modeling the emergence of HIV-1 drug resistance resulting from antiretroviral therapy: Insights from theoretical and numerical studies

Raimundo, Silvia Martorano; Yang, Hyun Mo; Venturino, Ezio; Massad, Eduardo
Fonte: Elsevier; Oxford Publicador: Elsevier; Oxford
Tipo: Artigo de Revista Científica
Português
The use of antiretroviral therapy has proven to be remarkably effective in controlling the progression of human immunodeficiency virus (HIV) infection and prolonging patient's survival. Therapy however may fail and therefore these benefits can be compromised by the emergence of HIV strains that are resistant to the therapy. In view of these facts, the question of finding the reason for which drug-resistant strains emerge during therapy has become a worldwide problem of great interest. This paper presents a deterministic HIV-1 model to examine the mechanisms underlying the emergence of drug-resistance during therapy. The aim of this study is to determine whether, and how fast, antiretroviral therapy may determine the emergence of drug resistance by calculating the basic reproductive numbers. The existence, feasibility and local stability of the equilibriums are also analyzed. By performing numerical simulations we show that Hopf bifurcation may occur. The model suggests that the individuals with drug-resistant infection may play an important role in the epidemic of HIV. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

‣ A molecular linkage map for Drosophila mediopunctata confirms synteny with Drosophila melanogaster and suggests a region that controls the variation in the number of abdominal spots

Laborda, P. R.; Gazaffi, R.; Garcia, A. A. F.; de Souza, A. P.
Fonte: Wiley-Blackwell; Malden Publicador: Wiley-Blackwell; Malden
Tipo: Artigo de Revista Científica
Português
The classic approach to gene discovery relies on the construction of linkage maps. We report the first molecular-based linkage map for Drosophila mediopunctata, a neotropical species of the tripunctata group. Eight hundred F2 individuals were genotyped at 49 microsatellite loci, resulting in a map that is approximate to 450 centimorgans long. Five linkage groups were detected, and the species' chromosomes were identified through cross-references to BLASTn searches and Muller elements. Strong synteny was observed when compared with the Drosophila melanogaster chromosome arms, but little conservation in the gene order was seen. The incorporation of morphological data corresponding to the number of central abdominal spots on the map was consistent with the expected location of a genomic region responsible for the phenotype on the second chromosome.; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

‣ Development of a recombinant fusion protein based on the dynein light chain LC8 for non-viral gene delivery

Toledo, Marcelo A. S.; Janissen, Richard; Favaro, Marianna T. P.; Cotta, Monica A.; Monteiro, Gabriel A.; Prazeres, Duarte Miguel F.; Souza, Anete P.; Azzoni, Adriano R.
Fonte: Elsevier; Amsterdam Publicador: Elsevier; Amsterdam
Tipo: Artigo de Revista Científica
Português
The low efficiency of gene transfer is a recurrent problem in DNA vaccine development and gene therapy studies using non-viral vectors such as plasmid DNA (pDNA). This is mainly due to the fact that during their traffic to the target cell's nuclei, plasmid vectors must overcome a series of physical, enzymatic and diffusional barriers. The main objective of this work is the development of recombinant proteins specifically designed for pDNA delivery, which take advantage of molecular motors like dynein, for the transport of cargos from the periphery to the centrosome of mammalian cells. A DNA binding sequence was fused to the N-terminus of the recombinant human dynein light chain LC8. Expression studies indicated that the fusion protein was correctly expressed in soluble form using E. coli BL21(DE3) strain. As expected, gel permeation assays found the purified protein mainly present as dimers, the functional oligomeric state of LC8. Gel retardation assays and atomic force microscopy proved the ability of the fusion protein to interact and condense pDNA. Zeta potential measurements indicated that LC8 with DNA binding domain (LD4) has an enhanced capacity to interact and condense pDNA, generating positively charged complexes. Transfection of cultured HeLa cells confirmed the ability of the LD4 to facilitate pDNA uptake and indicate the involvement of the retrograde transport in the intracellular trafficking of pDNA: LD4 complexes. Finally...