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‣ Incorporação de triciclo[6.2.1.02,7]undeca-4,9-dieno-3,6-diol como uma unidade de restrição conformacional de análogos peptídicos; Tricyclo[6.2.1.02,7] undeca-4,9- diene-3,6- diol incorporated in conformational constrained peptides analogues

Silva, Sandra Vieira da; Axt, Mariane; Costa, Valentim Emilio Uberti; Pohlmann, Adriana Raffin
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
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O objetivo deste trabalho é a obtenção de um derivado peptídico incorporado da unidade triciclo[6.2.1.02,7]undeca-4,9-dieno-3,6-diol, obtida a partir da reação de Diels-Alder do ciclopentadieno e da pbenzoquinona, seguida de redução. A condensação em solução do tripeptídeo (Gly-L-AlaGly) com o diol produz um derivado capaz de assumir uma conformação de folheto paralelo.; The aim of this work is the synthesis of a peptide analog incorporating the unit tricyclo[6.2.1.02,7]undeca-4,9-diene-3,6-diol, which was obtained from the Diels-Alder reaction of cyclopentadiene and p-benzoquinone, followed by a reduction reaction. The tripeptide (Gly-L-AlaGly) condensed with the diol gives a derivative able to assume a parallel b-sheet conformation.

‣ Clipping 2013-02-fevereiro

UFSC, Agecom
Fonte: Universidade Federal de Santa Catarina Publicador: Universidade Federal de Santa Catarina
Português
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Clipping de 01/02/2013: Secult / Edital Espaço Vivo 2013 / Lei de Licitações / ocupação dos espaços públicos sob responsabilidade da Secult / Centro de Cultura e Eventos / Teatro da UFSC / auditório da Reitoria / Fortalezas de Santa Cruz de Anhatomirim, São José de Ponta Grossa e Ratones Clipping de 02, 03 e 04/02/2013: Ataques a ônibus em Santa Catarina / Ingleses / Heron Felipe de Mello / Queimaduras / Hospital Celso Ramos / Hospital Universitário – HU / Secretaria de Estado da Saúde / Causas / Direito Penal / Penas estatais / Prisões / Função ressocializadora / Atuação do estado / Contenção da criminalidade / Políticas sociais e econômicas / Aparelho repressivo do Estado / Violação dos direitos humanos individuais e coletivos / Sistema de violência social / Política de segurança pública / Professor da UFSC, Matheus Felipe de Castro / Empresário Luiz Alvarenga Guidugli Junior / Falta de segurança em Florianópolis / Furtos constantes / Gastos com segurança / UFSC / Centro Acadêmico Livre de Engenharia da Mobilidade / Avanço das obras / Prédio da UFSC em Joinville / BR 101 Clipping de 05 a 14/02/2013: Exposição ARREDA nos Passos do Boi / Associação Cultural Arreda Boi da Barra da Lagoa / UFSC / Franklin Cascaes / Museu de Arqueologia e Etnologia da UFSC – MarquE / Compra do edifício Comercial Santa Clara / Igrejinha / Campus da Trindade / Segunda chamada / Sisu / Matrículas / Ostreicultura catarinense / Vazamento de óleo / Ilha de Santa Catarina / Prejuízos socioambientais / Reflexos econômicos / Autoridades sanitárias e ambientais / Suspensão de colheita e comercialização / Celesc / Punição de servidores omissos / Prejuízos indenizados / Roberto Pugliese / Homologação da BMW / Inovar-Auto / Ministério da Ciência e Tecnologia / Secretário de Desenvolvimento Tecnológico e Inovação / Ex-Reitor da UFSC Álvaro Prata / Inscrições / Curso Globalización y Teoría Social Latinoamericana / Professor Alejandro Marcos Bialakowsky / Facultad de Ciencias Sociales / Universidad de Buenos Aires / Vagas / Estudantes da UFSC / Fundação de Estudos e Pesquisas Sócio-Econômicos – Fepese / Palhoça / Secretaria Municipal de Educação e Cultura / Prefeitura Municipal / Concurso público / Campus Professor João David Ferreira Lima / Cariocas / Transferência da Eletrosul para Florianópolis / Gaúchos / Instalação da RBS / Paulistas / Maravilhas da Capital / Sonho de morar em Florianópolis / Onda de atentados no Estado / Violência urbana / Insegurança / Secretário Nacional de Políticas de Turismo Vinícius Lummertz / Prejuízos / Início do ano letivo / Vestibulando / Curso de Engenharia Ambiental da UFSC / Frustração / Caderno Vestibular / Feriadão de Carnaval / Restaurante Universitário / Pesquisa / Associação Catarinense de Engenheiros Sanitaristas e Ambientais – Acesa / Graduação na UFSC / Campeões do Vestibular / Adilto Mezzari Júnior / Curso de Medicina da UFSC / Primeiro lugar geral da Udesc / Delcio Castagnaro Filho / Primeiro lugar geral da UFSC / Reitora Roselane Neckel / Maurício Torquato / Primeiro lugar geral da Acafe / Curso de Engenharia de Produção Mecânica da UFSC / Leonardo de Andrade Mesquita / Primeiro lugar geral da UFRGS / Gustavo Benetti Delai / Primeiro lugar geral da UFPR / Dicas / Programa de Pós-Graduação em Antropologia Social da UFSC - PPGAS / Curso Antropologia e Psicanálise: Contribuição do pensamento de Guattari / Prefeito de Araranguá...

‣ Clipping 2014-02-fevereiro

UFSC, Agecom
Fonte: Universidade Federal de Santa Catarina Publicador: Universidade Federal de Santa Catarina
Tipo: Imagem
Português
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Clipping de 01, 02 e 03/02/2014: UFSC / Comissão Permanente do Vestibular – Coperve / Inscrição para processo seletivo / Vagas remanescentes do Vestibular 2014 / Campi de Florianópolis, Araranguá, Blumenau e Curitibanos / Curso de Tecnologia da Informação e Comunicação / Falecimento de João Francisco Vaz Sepetiba / Academia de Letras de Biguaçu / Revisor do jornal O Estado / Imprensa Universitária da UFSC / Mobilidade urbana na Grande Florianópolis / Avalanche migratória / Planejamento do espaço urbano / Qualidade de vida da população / Investimentos em infraestrutura, educação e saúde / Abordagens corajosas / Retirada da Ilha de equipamentos públicos / Sede administrativa do governo do Estado / Terminal Rita Maria / Transporte público de qualidade / Transporte marítimo / José Carlos Portella Nunes / Aniversário de 90 anos de Salim Miguel / Contista e romancista / Jornalista / Revista Manchete / Jornal do Brasil / Diretor da Editora da UFSC - EdUFSC / Fundação Franklin Cascaes / Título de Doutor Honoris Causa da UFSC / Prêmio Juca Pato / Prêmio Machado de Assis / Eglê Malheiros / Grupo Sul / Livraria Anita Garibaldi / Romance Primeiro de Abril, Narrativas da Cadeia / Pesquisadora do Programa de Pós-Graduação em Estudos da Tradução da UFSC...

‣ Clipping 2015-02-Fevereiro

UFSC, Agecom
Fonte: Universidade Federal de Santa Catarina Publicador: Universidade Federal de Santa Catarina
Tipo: Imagem
Português
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Clipping de 03/02/2015: Ano letivo / Festas / Patoloko Summer / Music Park / UFSC / Cineasta / Zeca Pires / Documentário Anauê / Nazismo em Santa Catarina / Depoimentos / Calouros / 2ª chamada para calouros / Florianópolis / Dengue Clipping de 04/02/2015: Charlie Hebdo / Edição histórica / Ensaio / Atentados / Edição histórica / Dirce Waltrick do Amarante / Professora do curso de Artes Cênicas / Caricatura / Humor / Riso / Aluguel / Imobiliárias / Udesc / Zeca Nunes Pires / Nazismo / Integralismo / Santa Catarina / Anauê / Documentário / Colonização alemã / Filme / Mosquito da dengue / Campus Party / Qualidade da água / Lagoa do Peri / Fundo Newton / Pimont Arquitetura / Vagas remanescentes / Vestibular Clipping de 05/02/2015: UFSC / Lista de aprovados / Vestibular / Recuperando vidas / Colégio Energia / Universidade Federal de Santa Catarina Clipping de 06/02/2015: Orgânicos / UFSC / Eletrosul / Feira / Gastronomia / Flores / Produtos orgânicos / Brinquedos educativos / Roupas artesanais / Performances culturais / Mesinha Gourmet / Empório / Nanotecnologia Clipping de 07 e 08/02/2015: Livro / Um Olhar sobre o Jornalismo / Petrolina / Sertão de PE Clipping de 09/02/2015: Terceira idade / Expectativa de vida / IBGE / Instituto Brasileiro de Geografia e Estatística / Eddy Frantov / Neti / Núcleo de Estudos da Terceira Idade / UFSC / Sônia Maria da Silva / Funcionária aposentada / RU / Restaurante Universitário / Recepcionista na Reitoria / Fernando Marcondes de Mattos / Roberto Costa / Líder estudantil / Engenharia / Escrache bar / DCE / Diretório Central dos Estudantes / Propague / Joinville / Campus da UFSC / Ônibus / Florianópolis / Matrícula / Colégio de Aplicação / Idosos / Santa Catarina / Rodrigo Gularte / Indonésia / Rede estadual de educação / Santa Catarina / Projeto fotográfico Clipping de 10/02/2015: Matrícula / Recepção dos calouros / Boas-vindas / UFSC / Veteranos / Centro Acadêmico de Letras / Piscina / CCE / Centro de Comunicação e Expressão / Letras / 1ª semana de integração da UFSC / Opinião / Fernando Anísio Batista / Sociólogo / Migrantes / Refugiados / Florianópolis / Melhores condições de vida / Grupo de Apoio aos Migrantes e Refugiados / Arquidiocese de Florianópolis / Udesc / Semana dos migrantes / Causas sociais / Gustavo Oliveira / Engenharia de Produção / Mariana Cassol / Economia/ Alunos da UFSC / Página Facebook / ‘Floripa invisível’ / Federação das Empresas Juniores de SC / Moradores de rua / Surdo / Surdo mais bonito das Américas / Concurso Mister Deaf / Tomaz Beche / Libras / Linguagem de sinais / Fábio Brüggemann / Floripa Invisível / Facebook / Veteranos / Florianópolis / Filmes nacionais / Mostra / Câmara de Graduação da UFSC / Sisu / Oficinas de Arte Clipping de 11/02/2015: Antonio Gavazzoni / Daiane Tramontini / Dissertação de mestrado / Direito / UFSC / Financiamento eleitoral de campanhas políticas / Ensino / Professor / Aluno / Servidor / Universidades públicas do Brasil / Recife / Florianópolis / Marcos Espíndola / Banda / Monbojó / Dance / Alexandre / Michele Diniz / UFSCtock / YouTube / Carro compartilhado / Alternativa / Futuro / Garagem / Ônibus / Transporte coletivo / Pedra Branca Cidade Criativa / Palhoça / PodShare / Compartilhamento de veículos / Engenharia Mecânica / Ciência da Computação / Física / Engenharia Elétrica / Design / Locação compartilhada / Congestionamento / Grande Florianópolis / Tecnologia / Fundação Certi / Embrapii / Empresa Brasileira de Pesquisa e Inovação Industrial / Fanem / Equipamentos / Neonatal / Gnatus / Equipamentos odontológicos / Obituário / Walter Antônio Machado / Bairro Abraão / Vó Zuma / Zuma Martins Machado / Universidade Federal de Santa Catarina / Saúde / Equipamentos quebrados / Hospitais públicos / Tomógrafos / Hospital Infantil Joana de Gusmão / Hospital Celso Ramos / Raio X / Regional de São José / Serviço Médico de Urgência / SAMU / Murilo Capella / Comissão de Saúde Pública da OAB / Cristina Pires / Herdeiros / Desigualdade / Tradução / Obra / Bourdieu...

‣ Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02

Sun,Xinyi; Hao,Long; Ma,Hua; Li,Tong; Zheng,Lan; Ma,Zhao; Zhai,Guoyin; Wang,Liqin; Gao,Shanglong; Liu,Xiaonan; Jia,Mengshi; Jia,Le
Fonte: Sociedade Brasileira de Microbiologia Publicador: Sociedade Brasileira de Microbiologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2013 Português
Relevância na Pesquisa
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The extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of EPS of P. eryngii SI-02 was 0.98 ± 0.05, 60.66 ± 5.14% higher than that of BHT. The results provide a reference for large-scale production of EPS by P. eryngii SI-02 in industrial fermentation and the EPS can be used as a potential antioxidant which enhances adaptive immune responses.

‣ Improvement of Influenza A/Fujian/411/02 (H3N2) Virus Growth in Embryonated Chicken Eggs by Balancing the Hemagglutinin and Neuraminidase Activities, Using Reverse Genetics

Lu, Bin; Zhou, Helen; Ye, Dan; Kemble, George; Jin, Hong
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /06/2005 Português
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The H3N2 influenza A/Fujian/411/02-like virus strains that circulated during the 2003-2004 influenza season caused influenza epidemics. Most of the A/Fujian/411/02 virus lineages did not replicate well in embryonated chicken eggs and had to be isolated originally by cell culture. The molecular basis for the poor replication of A/Fujian/411/02 virus was examined in this study by the reverse genetics technology. Two antigenically related strains that replicated well in embryonated chicken eggs, A/Sendai-H/F4962/02 and A/Wyoming/03/03, were compared with the prototype A/Fujian/411/02 virus. A/Sendai differed from A/Fujian by three amino acids in the neuraminidase (NA), whereas A/Wyoming differed from A/Fujian by five amino acids in the hemagglutinin (HA). The HA and NA segments of these three viruses were reassorted with cold-adapted A/Ann Arbor/6/60, the master donor virus for the live attenuated type A influenza vaccines (FluMist). The HA and NA residues differed between these three H3N2 viruses evaluated for their impact on virus replication in MDCK cells and in embryonated chicken eggs. It was determined that replication of A/Fujian/411/02 in eggs could be improved by either changing minimum of two HA residues (G186V and V226I) to increase the HA receptor-binding ability or by changing a minimum of two NA residues (E119Q and Q136K) to lower the NA enzymatic activity. Alternatively...

‣ Diverse Peptide Presentation of Rhesus Macaque Major Histocompatibility Complex Class I Mamu-A*02 Revealed by Two Peptide Complex Structures and Insights into Immune Escape of Simian Immunodeficiency Virus ▿

Liu, Jun; Dai, Lianpan; Qi, Jianxun; Gao, Feng; Feng, Youjun; Liu, Wenjun; Yan, Jinghua; Gao, George F.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /07/2011 Português
Relevância na Pesquisa
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Major histocompatibility complex class I (MHC I)-restricted CD8+ T-cell responses play a pivotal role in anti-human immunodeficiency virus (HIV) immunity and the control of viremia. The rhesus macaque is an important animal model for HIV-related research. Among the MHC I alleles of the rhesus macaque, Mamu-A*02 is prevalent, presenting in ≥20% of macaques. In this study, we determined the crystal structure of Mamu-A*02, the second structure-determined MHC I from the rhesus macaque after Mamu-A*01. The peptide presentation characteristics of Mamu-A*02 are exhibited in complex structures with two typical Mamu-A*02-restricted CD8+ T-cell epitopes, YY9 (Nef159 to -167; YTSGPGIRY) and GY9 (Gag71 to -79; GSENLKSLY), derived from simian immunodeficiency virus (SIV). These two peptides utilize similar primary anchor residues (Ser or Thr) at position 2 and Tyr at position 9. However, the central region of YY9 is different from that of GY9, a difference that may correlate with the immunogenic variance of these peptides. Further analysis indicated that the distinct conformations of these two peptides are modulated by four flexible residues in the Mamu-A*02 peptide-binding groove. The rare combination of these four residues in Mamu-A*02 leads to a variant presentation for peptides with different residues in their central regions. Additionally...

‣ DQB1*06:02 allele specific expression varies by allelic dosage, not narcolepsy status

lachmi, Karin Weiner; Lin, Ling; Kornum, Birgitte Rahbek; Rico, Tom; Lo, Betty; Aran, Adi; Mignot, Emmanuel
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Português
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The association of narcolepsy-cataplexy, a sleep disorder caused by the loss of hypocretin/orexin neurons in the hypothalamus, with DQA1*01:02-DQB1*06:02 is one of the tightest known single allele HLA associations. In this study, we explored genome wide expression in peripheral white blood cells of 50 narcolepsy versus 47 controls (half of whom were DQB1*06:02 positive) and found the largest differences between the groups to be in the signal from HLA probes. Further studies of HLA-DQ expression (mRNA and protein in a subset) in 125 controls and 147 narcolepsy cases did not reveal any difference, a result we explain by the lack of proper control of allelic diversity in Affymetrix HLA probes. Rather, a clear effect of DQB1*06:02 allelic dosage on DQB1*06:02 mRNA levels (1.65 fold) and protein (1.59 fold) could be demonstrated independent of the disease status. These results indicate that allelic dosage is transmitted into changes in heterodimer availability, a phenomenon that may explain increased risk for narcolepsy in DQB1*06:02 homozygotes versus heterozygotes.

‣ Genome-wide association study of HLA-DQB1*06:02 negative essential hypersomnia

Khor, Seik-Soon; Miyagawa, Taku; Toyoda, Hiromi; Yamasaki, Maria; Kawamura, Yoshiya; Tanii, Hisashi; Okazaki, Yuji; Sasaki, Tsukasa; Lin, Ling; Faraco, Juliette; Rico, Tom; Honda, Yutaka; Honda, Makoto; Mignot, Emmanuel; Tokunaga, Katsushi
Fonte: PeerJ Inc. Publicador: PeerJ Inc.
Tipo: Artigo de Revista Científica
Publicado em 16/04/2013 Português
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Essential hypersomnia (EHS), a sleep disorder characterized by excessive daytime sleepiness, can be divided into two broad classes based on the presence or absence of the HLA-DQB1*06:02 allele. HLA-DQB1*06:02-positive EHS and narcolepsy with cataplexy are associated with the same susceptibility genes. In contrast, there are fewer studies of HLA-DQB1*06:02 negative EHS which, we hypothesized, involves a different pathophysiological pathway than does narcolepsy with cataplexy. In order to identify susceptibility genes associated with HLA-DQB1*06:02 negative EHS, we conducted a genome-wide association study (GWAS) of 125 unrelated Japanese EHS patients lacking the HLA-DQB1*06:02 allele and 562 Japanese healthy controls. A comparative study was also performed on 268 HLA-DQB1*06:02 negative Caucasian hypersomnia patients and 1761 HLA-DQB1*06:02 negative Caucasian healthy controls. We identified three SNPs that each represented a unique locus— rs16826005 (P = 1.02E-07; NCKAP5), rs11854769 (P = 6.69E-07; SPRED1), and rs10988217 (P = 3.43E-06; CRAT) that were associated with an increased risk of EHS in this Japanese population. Interestingly, rs10988217 showed a similar tendency in its association with both HLA-DQB1*06:02 negative EHS and narcolepsy with cataplexy in both Japanese and Caucasian populations. This is the first GWAS of HLA-DQB1*06:02 negative EHS...

‣ Next Generation Sequencing Reveals the Association of DRB3*02:02 With Type 1 Diabetes

Erlich, Henry A.; Valdes, Ana Maria; McDevitt, Shana L.; Simen, Birgitte B.; Blake, Lisbeth A.; McGowan, Kim R.; Todd, John A.; Rich, Stephen S.; Noble, Janelle A.;
Fonte: American Diabetes Association Publicador: American Diabetes Association
Tipo: Artigo de Revista Científica
Português
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The primary associations of the HLA class II genes, HLA-DRB1 and HLA-DQB1, and the class I genes, HLA-A and HLA-B, with type 1 diabetes (T1D) are well established. However, the role of polymorphism at the HLA-DRB3, HLA-DRB4, and HLA-DRB5 loci remains unclear. In two separate studies, one of 500 subjects and 500 control subjects and one of 366 DRB1*03:01–positive samples from selected multiplex T1D families, we used Roche 454 sequencing with Conexio Genomics ASSIGN ATF 454 HLA genotyping software analysis to analyze sequence variation at these three HLA-DRB loci. Association analyses were performed on the two HLA-DRB loci haplotypes (DRB1-DRB3, -DRB4, or -DRB5). Three common HLA-DRB3 alleles (*01:01, *02:02, *03:01) were observed. DRB1*03:01 haplotypes carrying DRB3*02:02 conferred a higher T1D risk than did DRB1*03:01 haplotypes carrying DRB3*01:01 in DRB1*03:01/*03:01 homozygotes with two DRB3*01:01 alleles (odds ratio [OR] 3.4 [95% CI 1.46–8.09]), compared with those carrying one or two DRB3*02:02 alleles (OR 25.5 [3.43–189.2]) (P = 0.033). For DRB1*03:01/*04:01 heterozygotes, however, the HLA-DRB3 allele did not significantly modify the T1D risk of the DRB1*03:01 haplotype (OR 7.7 for *02:02; 6.8 for *01:01). These observations were confirmed by sequence analysis of HLA-DRB3 exon 2 in a targeted replication study of 281 informative T1D family members and 86 affected family-based association control (AFBAC) haplotypes. The frequency of DRB3*02:02 was 42.9% in the DRB1*03:01/*03:01 patients and 27.6% in the DRB1*03:01/*04 (P = 0.005) compared with 22.6% in AFBAC DRB1*03:01 chromosomes (P = 0.001). Analysis of T1D-associated alleles at other HLA loci (HLA-A...

‣ Identification of the Variations in the CPT1B and CHKB Genes Along with the HLA-DQB1*06:02 Allele in Turkish Narcolepsy Patients and Healthy Persons

Cingoz, Sultan; Agilkaya, Sinem; Oztura, Ibrahim; Eroglu, Secil; Karadeniz, Derya; Evlice, Ahmet; Altungoz, Oguz; Yilmaz, Hikmet; Baklan, Baris
Fonte: Mary Ann Liebert, Inc. Publicador: Mary Ann Liebert, Inc.
Tipo: Artigo de Revista Científica
Publicado em 01/04/2014 Português
Relevância na Pesquisa
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Background: The HLA-DQB1*06:02 allele across all ethnic groups and the rs5770917 variation between CPT1B and CHKB genes in Japanese and Koreans are common genetic susceptibility factors for narcolepsy. This comprehensive genetic study sought to assess variations in CHKB and CPT1B susceptibility genes and HLA-DQB1*06:02 allele status in Turkish patients with narcolepsy and healthy persons. Methods: CHKB/CPT1B genes were sequenced in patients with narcolepsy (n=37) and healthy persons (n=100) to detect variations. The HLA-DQB1*06:02 allele status was determined by sequence specific polymerase chain reaction. Results: The HLA-DQB1*06:02 allele was significantly more frequent in narcoleptic patients than in healthy persons (p=2×10−7) and in patients with narcolepsy and cataplexy than in those without (p=0.018). The mean of the multiple sleep latency test, sleep-onset rapid eye movement periods, and frequency of sleep paralysis significantly differed in the HLA-DQB1*06:02–positive patients. rs5770917, rs5770911, rs2269381, and rs2269382 were detected together as a haplotype in three patients and 11 healthy persons. In addition to this haplotype, the indel variation (rs144647670) was detected in the 5′ upstream region of the human CHKB gene in the patients and healthy persons carrying four variants together. Conclusion: This study identified a novel haplotype consisting of the indel variation...

‣ Protective Effect of the HLA-DRB1*13:02 Allele in Japanese Rheumatoid Arthritis Patients

Oka, Shomi; Furukawa, Hiroshi; Kawasaki, Aya; Shimada, Kota; Sugii, Shoji; Hashimoto, Atsushi; Komiya, Akiko; Fukui, Naoshi; Ito, Satoshi; Nakamura, Tadashi; Saisho, Koichiro; Katayama, Masao; Tsunoda, Shinichiro; Sano, Hajime; Migita, Kiyoshi; Suda, Akik
Fonte: Public Library of Science Publicador: Public Library of Science
Tipo: Artigo de Revista Científica
Publicado em 09/06/2014 Português
Relevância na Pesquisa
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Rheumatoid arthritis (RA) is a chronic systemic inflammatory disease. Certain HLA-DRB1 “shared-epitope” alleles are reported to be positively associated with increased RA susceptibility, whereas some of the other alleles may be negatively associated. However, studies on the latter are rare. Here, we focus on the protective effects of DRB1 alleles in Japanese RA patients in an association study. Relative predispositional effects (RPE) were analyzed by sequential elimination of carriers of each allele with the strongest association. The protective effects of DRB1 alleles were investigated in patients stratified according to whether they possessed anti-citrullinated peptide antibodies (ACPA). The DRB1*13:02 allele was found to be negatively associated with RA (P = 4.59×10−10, corrected P (Pc) = 1.42×10−8, odds ratio [OR] 0.42, 95% CI 0.32–0.55, P [RPE] = 1.27×10−6); the genotypes DRB1*04:05/*13:02 and *09:01/*13:02 were also negatively associated with RA. The protective effect of *13:02 was also present in ACPA-positive patients (P = 3.95×10−8, Pc = 1.22×10−6, OR 0.42, 95%CI 0.31–0.58) whereas *15:02 was negatively associated only with ACPA-negative RA (P = 8.87×10−5, Pc = 0.0026, OR 0.26...

‣ Identification of a novel HLA-A*02:01-restricted cytotoxic T lymphocyte epitope derived from the EML4-ALK fusion gene

YOSHIMURA, MAYUKO; TADA, YOSHITAKA; OFUZI, KAZUYA; YAMAMOTO, MASAKAZU; NAKATSURA, TETSUYA
Fonte: D.A. Spandidos Publicador: D.A. Spandidos
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
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Cancer immunotherapy is a promising new approach to cancer treatment. It has been demonstrated that a high number of tumor-specific cytotoxic T cells (CTLs) is associated with increased disease-specific survival in lung cancer patients. Identification of superior CTL epitopes from tumor antigens is essential for the development of immunotherapy for malignant tumors. The EML4-ALK fusion gene was recently identified in a subset of non-small cell lung cancers (NSCLCs). In this study we searched for HLA-A*02:01- and HLA-A*24:02-restricted epitopes derived from EML4-ALK by screening predicted EML4-ALK-derived candidate peptides for the induction of tumor-reactive CTLs. Nine EML4-ALK-derived peptides were selected by a computer algorithm based on a permissive HLA-A*02:01 or HLA-A*24:02 binding motif. One of the nine peptides induced peptide-specific CTLs from human peripheral blood mononuclear cells. We were able to generate a peptide-specific CTL clone. This CTL clone specifically recognized peptide-pulsed T2 cells and H2228 cells expressing HLA-A*02:01 and EML4-ALK that had been treated with IFN-γ 48 h prior to examination. CTL activity was inhibited by an anti-HLA-class I monoclonal antibody (W6/32), consistent with a class I-restricted mechanism of cytotoxicity. These results suggest that this peptide (RLSALESRV) is a novel HLA-A*02:01-restricted CTL epitope and that it may be a new target for antigen-specific immunotherapy against EML4-ALK-positive cancers.

‣ DQB1*06:02-Associated Pathogenic Anti-Myelin Autoimmunity in Multiple Sclerosis-Like Disease: Potential Function of DQB1*06:02 as a Disease-Predisposing Allele

Kaushansky, Nathali; Ben-Nun, Avraham
Fonte: Frontiers Media S.A. Publicador: Frontiers Media S.A.
Tipo: Artigo de Revista Científica
Publicado em 16/10/2014 Português
Relevância na Pesquisa
26.408286%
Susceptibility to multiple sclerosis (MS) has been linked mainly to the HLA-DRB1 locus, with the HLA-DR15 haplotype (DRB1*1501-DQA1*0102-DQB1*0602-DRB5*0101) dominating MS risk in Caucasians. Although genes in the HLA-II region, particularly DRB1*1501, DQA1*0102-DQB1*0602, are in tight linkage disequilibrium, genome-wide-association, and gene candidate studies identified the DRB1*15:01 allele as the primary risk factor in MS. Many genetic and immune-functional studies have indicated DRB1*15:01 as a primary risk factor in MS, while only some functional studies suggested a disease-modifying role for the DRB5*01 or DQB1*06 alleles. In this respect, the susceptibility of DRB1*15:01-transgenic (Tg) mice to myelin basic protein- or myelin oligodendrocyte glycoprotein-induced MS-like disease is consistent with primary contribution of DRB1*15:01 to HLA-DR15+ MS. The studies summarized here show that susceptibility to MS-like disease, induced in HLA-“humanized” mice by myelin oligodendrocytic basic protein or by the proteolipid protein, one of the most prominent encephalitogenic target antigens implicated in human MS, is determined by DQB1*06:02, rather than by the DRB1*15:01 allele. These findings not only offer a rationale for a potential role for DQB1*06:02 in predisposing susceptibility to MS...

‣ Liraglutide reduces lipid accumulation in steatotic L-02 cells by enhancing autophagy

ZHOU, SHI-WEI; ZHANG, MAN; ZHU, MIN
Fonte: D.A. Spandidos Publicador: D.A. Spandidos
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
26.455625%
Simple hepatic steatosis is the early stage of non-alcoholic fatty liver disease and is recognized as a benign process. Previous studies show that glucagon-like peptide-1 has great potential in improving hepatic steatosis. Recent data have revealed that inhibiting autophagy exacerbates lipid accumulation in hepatocytes. Therefore, the present study aimed to determine the effects of liraglutide (LG) on simple hepatic steatosis and the possible role of autophagy. Firstly, steatotic L-02 cells were induced by incubating L-02 cells with 1 mmol/l free fatty acid (FFA) mixture (oleic acid and palmitic acid at a molar ratio of 2:1) for 24 h. Intracellular lipid accumulation, cell viability, oxidative stress and apoptosis were evaluated. Secondly, steatotic L-02 cells were treated with 10 or 100 nmol/l LG, 100 nmol/l LG plus 3-methyladenine (3-MA), or rapamycin for 24 h, and then lipid accumulation was measured. Next, the degree of lipid accumulation and the intensity of autophagy were assessed. Oil red O staining and triglyceride quantification demonstrated notable steatosis in L-02 cells following exposure to 1 mmol/l FFA mixture for 24 h. There was no significant cytotoxicity, oxidative stress or apoptosis in steatotic L-02 cells. Treatment with 100 nmol/l LG reduced lipid accumulation in steatotic L-02 cells and increased the mRNA levels of microtubule-associated protein 1 light chain 3B. Additionally...

‣ The new DoD Instruction 5000.02: an analysis of the efficiencies to be gained

Kusnerak, Michael
Fonte: Monterey, California: Naval Postgraduate School Publicador: Monterey, California: Naval Postgraduate School
Tipo: Tese de Doutorado
Português
Relevância na Pesquisa
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Approved for public release; distribution is unlimited; In November 2013, the Deputy Secretary of Defense issued a memorandum titled Defense Acquisition, which stated that the 2008 version of the DOD Instruction (DODI) 5000.02 required revision in order to create an acquisition policy environment that will achieve greater efficiency and productivity in defense spending, and effectively implement the Defense Department’s Better Buying Power initiatives. A year later, the Interim DODI 5000.02 was issued and required for use, and the final instruction was signed into effect in January 2015. While the new instruction canceled six directive-type memorandums and reportedly incorporated them in the new instruction, efficiencies are not easily identified. This research provides an analysis of the new DODI 5000.02 and the Better Buying Power initiatives. It was discovered that the efficiencies to be gained have not been properly identified. It is left for interpretation what those efficiencies are. If they are decreased costs and schedules, the metrics used in this research, they have not been achieved to date. Proper metrics for these efficiencies need to be formulated and articulated to the defense acquisition workforce.; ; Major, United States Marine Corps

‣ Analysis of HLA A*02 Association with Vaccine Efficacy in the RV144 HIV-1 Vaccine Trial

Gartland, Andrew J.; Li, Sue; McNevin, John; Tomaras, Georgia D.; Gottardo, Raphael; Janes, Holly; Fong, Youyi; Morris, Daryl; Geraghty, Daniel E.; Kijak, Gustavo H.; Edlefsen, Paul T.; Frahm, Nicole; Larsen, Brendan B.; Tovanabutra, Sodsai; Sanders-Buell
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /08/2014 Português
Relevância na Pesquisa
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The RV144 HIV-1 vaccine trial demonstrated partial efficacy of 31% against HIV-1 infection. Studies into possible correlates of protection found that antibodies specific to the V1 and V2 (V1/V2) region of envelope correlated inversely with infection risk and that viruses isolated from trial participants contained genetic signatures of vaccine-induced pressure in the V1/V2 region. We explored the hypothesis that the genetic signatures in V1 and V2 could be partly attributed to selection by vaccine-primed T cells. We performed a T-cell-based sieve analysis of breakthrough viruses in the RV144 trial and found evidence of predicted HLA binding escape that was greater in vaccine versus placebo recipients. The predicted escape depended on class I HLA A*02- and A*11-restricted epitopes in the MN strain rgp120 vaccine immunogen. Though we hypothesized that this was indicative of postacquisition selection pressure, we also found that vaccine efficacy (VE) was greater in A*02-positive (A*02+) participants than in A*02− participants (VE = 54% versus 3%, P = 0.05). Vaccine efficacy against viruses with a lysine residue at site 169, important to antibody binding and implicated in vaccine-induced immune pressure, was also greater in A*02+ participants (VE = 74% versus 15%...

‣ Avaliação da qualidade do programa de acompanhamento do crescimento e desenvolvimento de crianças de 0 a 1 ano em unidades básicas de saúde da Ceilândia nº 01 e nº 02; Avaliação da qualidade do programa de acompanhamento do crescimento e desenvolvimento de crianças de zero a um ano em unidades básicas de saúde da Ceilândia número um e número dois

Dias, Elizângela Gonçalves; Maia, Karliane Brito
Fonte: Universidade Católica de Brasília Publicador: Universidade Católica de Brasília
Tipo: Trabalho de Conclusão de Curso Formato: Texto
Português
Relevância na Pesquisa
26.408286%
Este trabalho teve como objetivo avaliar o Programa de Atenção Integral à Saúde da Criança (PAISC) das Unidades Básicas da Regional da Ceilândia, Centro de Saúde (CS) no. 1 e 2 por meio de questionário semi-estruturado e observação participante em consultório. O estudo incluiu o total de 28 mães. No questionário aplicado às mães, abordou-se seu grau de conhecimento quanto ao programa e sua percepção sobre o atendimento. Na observação participante, avaliou-se o atendimento do médico e do enfermeiro, tendo-se como base o Manual do Crescimento e Desenvolvimento (CD). No CSC nº. 01 e 02, 42,86% das mães não sabiam o que é CD. No CSC nº. 01, 100% das entrevistadas responderam que houve palestra de CD, enquanto no CS nº. 02, 35,71%. Não souberam interpretar o gráfico do cartão da criança 64,29% e 50,71%, no CS nº01 e CS nº02, respectivamente. Na observação participante, registrou-se acolhimento físico-afetivo para com 14,29% das mães do CS nº.1 e 28,57% das mães do CS nº. 02. Observou-se, ainda, que a análise e explicação das curvas do gráfico foram dadas a 50% das mães no CS nº.01 e a 42,86%, no CS nº. 02. Exame físico completo não foi observado nos CS nº01 e CS nº02. Exame físico incompleto foi realizado em 44...

‣ Primary Cosmic Ray Proton Flux Measured by AMS-02

Consolandi, C.; Collaboration, on Behalf of the AMS-02
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
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The Alpha Magnetic Spectrometer (AMS-02) is a high energy particle detector designed to study origin and nature of cosmic rays up to a few TV from space. It was installed on the International Space Station (ISS) on May 19, 2011. During the first two years of operation AMS-02 performed precise measurements of the proton flux. In the low rigidity range, from 1 GV to 20 GV, the proton flux was daily measured with a statistical error less than 1%. In the same rigidity range a gradual decrease due to Solar modulation effect and transit variations due to Solar Flares and Coronal Mass Ejection were also observed. In the rigidity range from 20 GV up to 100 GV instead, AMS-02 data show no drastic variation and the results are consistent with other experiments. Above 100 GV, AMS-02 proton flux exhibits a single power low behavior with no fine structures nor brakes.; Comment: Presented at the 10th International Symposium on Cosmology and Particle Astrophysics (CosPA2013) Honolulu, Hawai'i, November 12-15, 2013

‣ Implications of the AMS-02 positron fraction in cosmic rays

Yuan, Qiang; Bi, Xiao-Jun; Chen, Guo-Ming; Guo, Yi-Qing; Lin, Su-Jie; Zhang, Xinmin
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
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The AMS-02 collaboration has just released its first result of the cosmic positron fraction $e^+/(e^-+e^+)$ with high precision up to $\sim 350$ GeV. The AMS-02 result shows the same trend with the previous PAMELA result, which requires extra electron/positron sources on top of the conventional cosmic ray background, either from astrophysical sources or from dark matter annihilation/decay. In this paper we try to figure out the nature of the extra sources by fitting to the AMS-02 $e^+/(e^-+e^+)$ data, as well as the electron and proton spectra by PAMELA and the $(e^-+e^+)$ spectrum by Fermi and HESS. We adopt the GALPROP package to calculate the propagation of the Galactic cosmic rays and the Markov Chain Monte Carlo sampler to do the fit. We find that the AMS-02 data have implied essential difference from the PAMELA data. There is {\rm tension} between the AMS-02 $e^+/(e^-+e^+)$ data and the Fermi/HESS $(e^-+e^+)$ spectrum, that the AMS-02 data requires less contribution from the extra sources than Fermi/HESS. Then we redo the fit without including the Fermi/HESS data. In this case both the pulsars and dark matter annihilation/decay can explain the AMS-02 data. The pulsar scenario has a soft inject spectrum with the power-law index $\sim 2$...