Página 11 dos resultados de 20101 itens digitais encontrados em 0.007 segundos

‣ Sertoli cell expression of galectin-l and -3 and accessible binding sites in normal human testis and Sertoli cell only-syndrome

Wollina, U.; Schreiber, G.; Görnig, M.; Feldrappe, S.; Burchert, M.; Gabius, H.J.
Fonte: Murcia : F. Hernández Publicador: Murcia : F. Hernández
Tipo: Artigo de Revista Científica Formato: application/pdf
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Galectins are vertebrate lectins interacting with B-galactosides and derivates thereof such as blood group A, B and H determinants. The expression of galectin-l and -3 and galectin-specific binding sites by human Sertoli cells was analyzed in normal human testis and Sertoli cell only-syndrome (SCOS). Staining intensity was scored semiquantitatively on a 4-grade scale. Sertoli cells in normal testes displayed a moderate cytoplasmic and weak nuclear staining for galectin-lspecific binding sites. Galectin-3-specific binding sites were expressed in Sertoli cells less intensely than accessible ligands for galectin-l (mean score 2.25 for galectin-l and 1.50 for galectin-3). Germ cells were only weakly reactive. Tubular walls were negative for both classes of galectin-specific binding sites. In SCOS, galectin-l binding was moderate to strong and more pronounced than galectin-3 binding by Sertoli cells (mean scores 4.00 and 2.25). Tubular walls were negative for galectin-staining. The ratio for galectin-1-1 galectin-3-specific binding (staining score ratio) was 1.50 form normal testis and 1.78 for SCOS disclosing a relative increase of galectin-3 binding sites in the latter. Staining with galectin-l- and -3-specific antisera showed a strong cytoplasmic galectin-l immunoreactivity in Sertoli cells of normal and SCOS testis (score 4.00 for both). Anti-galectin-3 did not stain Sertoli cells or germ cells in normal testis. Only Leydig cells were labeled (score 3.00). In SCOS a weak to moderate nuclear staining of Sertoli cells was noted (score 2.00). Galectin-3 expression and galectin-l-specific binding sites were found to be increased in Sertoli cells of SCOS. This modulation of reactivity can have implications for Sertoli cell interactions with galectinreactive extracellular matrix components like laminin and for anti-apoptotic effects.

‣ Turismo y accesibilidad: una visión global sobre la situación de España; Tourism and accessibility. A global vision about the situation of Spain

Domínguez Vila, Trinidad; Fraiz Brea, José Antonio; Alén González, María Elisa
Fonte: Murcia, Universidad de Murcia, Servicio de Publicaciones Publicador: Murcia, Universidad de Murcia, Servicio de Publicaciones
Tipo: Artigo de Revista Científica Formato: application/pdf
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El turismo es una actividad de alta relevancia ya no solo a nivel de rentabilidad financiera, sino también en relación a la rentabilidad social. En base a ello y a la importancia que los nuevos segmentos están a tener en un mercado tan masificado que demanda productos diferenciados de forma continua, se realiza una visión sobre el turismo accesible centrado en el colectivo de personas con discapacidad así como en los beneficiarios directos, como el segmento de mayores o sénior, familias, embarazadas y personas con capacidades restringidas temporalmente. Para ello, se realiza una contextualización teórica, así como una visión global de la accesibilidad y sus principales componentes y repercusiones en España y Europa, para mostrar, grosso modo, su relevancia, las oportunidades de mercado que se generan así como las características y hábitos de comportamiento de los turistas españoles con discapacidad.; ABSTRACT The tourism is an activity of high relevance to for the financial profitability and the social profitability too. In base to this and to the importance that the new segments have on a mass market that need different products. We realizes a vision on the accessible tourism centered in the community of people with disability and in the beneficiaries direct...

‣ HTML5 support for an accessible user-video-interaction on the Web

Moreno, Lourdes; Martínez, Paloma; Iglesias, Ana; González, María
Fonte: Springer Publicador: Springer
Tipo: info:eu-repo/semantics/acceptedVersion; info:eu-repo/semantics/conferenceObject; info:eu-repo/semantics/bookPart Formato: application/pdf
Publicado em //2011 Português
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Multimedia content covers the Web, and we should provide access to all people. For this reason, it is very important to take into account accessibility requirements in the player to avoid barriers and to ensure access to this multimedia content as well as their resources. One of the most frequent barriers is the technological obstacle: the necessity for the user to install the required plug-ins in to order to access video. The new standard HTML5 provides a solution to this problem. However, it does not fully support accessibility requirements of W3C standards, including WCAG and interaction requirement of UAAG. This paper introduces an overall study of this new standard in relation to accessibility requirements for the players as well as an accessible HTML5 Media Player.; This research work is supported by the Research Network MAVIR (S2009/TIC-1542 (www.mavir.net/)), GEMMA (TSI-020302-2010-141) and SAGAS (TSI-020100-2010- 184) research projects.

‣ An Accessible Chat Prototype for Screen Reader Users in Mobile Devices

Calvo, Rocío; Iglesias, Ana; Moreno, Lourdes
Fonte: Springer Publicador: Springer
Tipo: info:eu-repo/semantics/acceptedVersion; info:eu-repo/semantics/conferenceObject; info:eu-repo/semantics/bookPart; info:eu-repo/semantics/conferenceObject Formato: application/pdf
Publicado em 21/07/2013 Português
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Chats present accessibility problems for screen reader users. This work presents a prototype of an accessible chat for Mobile Devices (MD). The main aim of this research is to remove the accessibility barriers that screen reader users face when they use a chat in a MD. Thus, this prototype is based on the requirements which have been elicited considering background research and with the use of Software Engineering (SE) and Human Computer Interaction (HCI) methods as well as accessibility standards and guidelines.; This research work has been partially supported by MA2VICMR (S2009/TIC-1542) and MULTIMEDICA (TIN2010-20644-C03-01); [Poster] 15th International Conference on Human–Computer Interaction (HCI International 2013), Las Vegas (Nevada, USA), 21–26 July 2013

‣ SEMA4A : a knowledge base for accessible evacuation and alert notifications in emergencies

Onorati, Teresa
Fonte: Universidade Carlos III de Madrid Publicador: Universidade Carlos III de Madrid
Tipo: Tese de Doutorado
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When an emergency occurs or is going to occur, the aim of organizations and agencies involved in the response phase is to restore quickly a safe situation and reduce the number of victims and damages. The notification of information about the kind of emergency, its characteristics, the location of safe places and available procedures for reaching them has a crucial role in order to facilitate the evacuation of citizens. Several organizations and agencies have been promoting the development of Information Technology (IT) tools, called Emergency Response Information Systems (ERIS) for the management of the activities performed in response to the emergencies. In particular, these systems provide modules for collecting, updating and notifying information about imminent disasters to potential affected people. Such notifications can be communicated through different channels, like websites, emails, text or voice messages. But to effectively inform people about an emergency, the notifications should be adapted automatically to each user’s profile (e.g. functional or contextual disabilities, elderly, children), the kind of emergency (e.g. typhoon, earthquake, tornado), the communication channel (e.g. PDAs, smartphones, pagers) and any other exceptional circumstances (e.g. interrupted roads...

‣ Accessible Chats for Computer Supported Collaborative Learning Environments in Mobile Devices (Doctoral Consortium),

Calvo, Rocío
Fonte: IEEE. The Institute Of Electrical And Electronics Engineers, Inc Publicador: IEEE. The Institute Of Electrical And Electronics Engineers, Inc
Tipo: info:eu-repo/semantics/acceptedVersion; info:eu-repo/semantics/conferenceObject; info:eu-repo/semantics/bookPart
Publicado em /05/2013 Português
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The use of chats in Mobile Devices (MD) for learning environments is being increased in the last decade. However, they present many accessibility barriers that prevent people from using them. As a result, some people do not have the same opportunities to learn. This research aims to solve the accessibility barriers of chats in learning environments for its use in MDs. Thus, this paper presents the proposal of an model-based design and the strategy development process to create an accessible chat; This research work has been partially supported by the Regional Government of Madrid under the Research Network MA2VICMR (S2009/TIC-1542) and by the Spanish Ministry of Economy under the project MULTIMEDICA (TIN2010-20644-C03-01); Proceedings of: Seventh IEEE International Conference on Research Challenges in Information Science. Took place May 29-31, 2013 in Paris (France). The event Web site is http://www.rcis-conf.com/rcis2013/

‣ Are chats and forums accessible in e-learning systems?: a heuristic evaluation comparing four learning content management systems

Calvo, Rocío; Gil, Almudena; Iglesias, Beatriz; Iglesias, Ana
Fonte: ACM Publicador: ACM
Tipo: info:eu-repo/semantics/acceptedVersion; info:eu-repo/semantics/conferenceObject Formato: application/pdf
Publicado em 01/07/2013 Português
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This paper presents a heuristic evaluation of accessibility of the Computer Supported Collaborative Learning (CSCL) tools: chat and forums in four Learning Content Management Systems (LCMS): Moodle, ATutor, dotLRN and Claroline. Moreover, some recommendations are offered in order to improve the accessibility of the tools; This study has been partially funded by the MA2VICMR (S2009/TIC-1542) research project; [Poster] 18th ACM conference on Innovation and technology in computer science education (ITiCSE'13), 1-3, July 2013, Canterbury (England)

‣ Using accessible digital resources for teaching database design: towards an inclusive distance learning proposal

Moreno, Lourdes; Iglesias, Ana; Castro, Elena; Martínez, Paloma
Fonte: ACM Publicador: ACM
Tipo: info:eu-repo/semantics/acceptedVersion; info:eu-repo/semantics/conferenceObject; info:eu-repo/semantics/bookPart Formato: application/pdf
Publicado em 30/06/2008 Português
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This paper introduces a pilot experience in teaching database using accessible digital resources in 3er course of Computer Science degree at Universidad Carlos III de Madrid. A platform containing learning material in different formats (video, audio, slides presentation) has been designed allowing students accessing resources as well as to be evaluated by means of tests. Preliminary results show that 46.81% of the students have already interacted with the system and 97.12% of the students passed the tests.; [Proceedings of] 13th Annual Conference on Innovation and Technology in Computer Science Education (ITiCSE'08), Madrid, Spain, June 30-July 2, 2008

‣ Using accessible digital resources for teaching database design: towards an inclusive distance learning proposal

Moreno, Lourdes; Iglesias, Ana; Castro, Elena; Martínez, Paloma
Fonte: ACM Publicador: ACM
Tipo: info:eu-repo/semantics/acceptedVersion; info:eu-repo/semantics/conferenceObject; info:eu-repo/semantics/article Formato: application/pdf
Publicado em 30/06/2008 Português
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This paper introduces a pilot experience in teaching database using accessible digital resources in 3er course of Computer Science degree at Universidad Carlos III de Madrid. A platform containing learning material in different formats (video, audio, slides presentation) has been designed allowing students accessing resources as well as to be evaluated by means of tests. Preliminary results show that 46.81% of the students have already interacted with the system and 97.12% of the students passed the tests.; [Proceedings of] 13th Annual Conference on Innovation and Technology in Computer Science Education (ITiCSE'08), Madrid, Spain, June 30-July 2, 2008

‣ Accessible Interfaces for Educational Multimedia Contents

Moreno, Lourdes; Iglesias, Ana; Martínez, Paloma; Ruiz-Mezcua, Belén
Fonte: Universidade Carlos III de Madrid Publicador: Universidade Carlos III de Madrid
Tipo: info:eu-repo/semantics/acceptedVersion; info:eu-repo/semantics/conferenceObject Formato: application/pdf
Publicado em 01/07/2008 Português
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The use of technology is growing in every field of education, and not only in the education of disabled students but also as a learning resource for everybody. The teachers are more and more introducing these digital contents in their lessons and there are many resources related to learning on internet.. If an equal access to these resources is guaranteed, then we can avoid students feeling that their learning capacity is limited due to a possible inaccessibility to them. Inclusive methodologies have to be followed to reach these objectives applying the standards such as Web Content Accessibility Guideline (WCAG) and rules of accessibility in the design and development of web pages, technical supporting, software, author tools, etc. This papers describes a practical case with two accessible interfaces of multimedia resources implemented with Synchronized Multimedia Integration Language (SMIL.); Proceedings of: Workshop on Advanced Learning Technologies for Disabled and Non-Disabled People (WALTD 2008), July 1st-July 5th, 2008, Santander, Cantabria, Spain, in conjunction with the 8th IEEE International Conference on Advanced Learning Technologies (ICALT 2008)

‣ Web-Accessible G-Spectrum Simulator with On-line Montecarlo for Voluminous and Shielded G-sources: First Results of Experimental Validation

BERLIZOV Andrey; DREHER Raymond
Fonte: ELSEVIER SCIENCE BV Publicador: ELSEVIER SCIENCE BV
Tipo: Articles in Journals Formato: Printed
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A modelling approach for a quick and accurate simulation of ¿-spectra from volume and shielded sources is described. It is based on the combination of two web-accessible modelling tools, the Easy Monte Carlo (EMC) and the Gamma Spectrum Generator (GSG), which have been recently developed within the European Commission's Nucleonica portal. The high performance of the simulation was achieved by the use of a next-event estimator for the photon flux calculations and an extensive database of the pre-calculated detector responses for the flux-to-pulse height distribution conversion. The paper presents the first results of the experimental validation of this approach performed with a 10% High Purity Germanium (HPGe) detector and shielded/unshielded reference ¿-sources.; JRC.E-Institute for Transuranium Elements (Karlsruhe)

‣ Mali : Transport Support to Sustainable Economic Growth

World Bank
Fonte: Washington, DC Publicador: Washington, DC
Tipo: Economic & Sector Work :: Other Infrastructure Study; Economic & Sector Work
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This Economic and Sector Work (ESW) is consistent with the objectives laid out for the transport sector in the Poverty Reduction Strategy Paper (PRSP), and the Country Assistance Strategy (CAS), with its two primary objectives focused on strengthening existing reforms, and, helping define a forthcoming reform program. The proposed transport sector strategy relies on three pillars to: 1) promote sustainable development of transport infrastructure, ensuring adequate allocation of financial, and human resources to infrastructure maintenance; 2) increase transport sector efficiency, through sound market, and fiscal policies that support the rapid modernization of Mali's transport companies; and, 3) support cross sectoral initiatives, primarily in the areas of economic competitiveness, road safety, rural poverty alleviation, and in addition, to support HIV/AIDS prevention, and health services accessibility. Since transport costs represent a significant share of the imported costs of intermediary goods used in building up Mali's export capacity...

‣ The Ethics, Feasibility, Opportunities and Challenges of Adopting a Facility Accessibility Design Standard (FADS) for Queen’s University

Tan, REBECCA K
Fonte: Quens University Publicador: Quens University
Tipo: Tese de Doutorado
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This research project seeks out the underlying structural and institutionalized barriers that threaten the development of a Facility Accessibility Design Standard (FADS) for Queen’s University, and focuses on processes as the unit of analysis rather than the content of a FADS itself. In essence, it is the cultural components of campus planning that are examined in depth; why it is that a FADS has been adopted and implemented at only one university in Ontario, Brock University, and what barriers have prevented other institutions and Queen’s from developing one. Because culture is the focus of this research, two literature reviews were conducted to demonstrate how Foucauldian planning theory and the theories of disablement overlap in planning practices that contradict professional planning ethics, but are so entrenched in campus institutions that it becomes difficult to reverse. Furthermore, the research reflects findings from six key informant interviews (four with individuals in decision-making positions at Queen’s and two with individuals who led the FADS development process at Brock), a walking tour of Brock’s campus, a visioning workshop with members of the Queen’s community, and a review of key policy documents. Ultimately...

‣ Planejamento estrategico : uma ferramenta acessivel a Microempresa; Strategic planning: an accessible tool for a small business

Jose Roberto Leite dos Santos
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 21/12/2004 Português
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Este estudo de caso procura mostrar que o Planejamento Estratégico pode ser um instrumento adequado e acessível para aplicação em uma microempresa e gerar beneficios de curto e longo prazo, necessários para melhorar suas condições de sobrevivência. Para que esta aplicação pudesse acontecer na empresa pesquisada, foi necessário encontrar uma solução visando a superação dos obstáculos e das dificuldades encontradas, as quais se revelaram semelhantes às já apontadas em outros trabalhos envolvendo empresas de pequeno porte. Dentre esses obstáculos destacam-se a gerência por intuição, o desconhecimento de técnicas administrativas e a sobrecarga de trabalhos exerci da pelo responsável, em atividades operacionais do dia a dia da empresa. Esse conjunto de fatores ocasiona uma crônica falta de tempo para a dedicação em atividades de aprendizado administrativo, formando um ciclo vicioso que contribui para os elevados índices de mortalidade desse tipo de empresa. Realiza-se na empresa pesquisada a quebra desse ciclo com a aplicação do planejamento estratégico conforme a metodologia Certo e Peter (1993), utilizando-se de uma prática simplificadora, chamada de "Sistemática da Ponderação de Fatores (SPF)", aqui proposta. Essa sistemática teve como objetivo solucionar o problema básico da falta de tempo do empresário...

‣ Uma ferramenta de autoria síncrona acessível para cegos: um estudo de caso no curso PROINESP; An authoring tool for synchronous accessible for the blind: a case study in the current PROINESP

Bärwaldt, Regina; Santarosa, Lucila Maria Costi; Passerino, Liliana Maria
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
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O artigo apresenta uma alternativa como forma de viabilizar a inclusão digital, social e educacional dos cegos através das TIC’s em ambientes virtuais de aprendizagem, permitindo canais diferenciados de interação social. Um dos fatores, senão o principal é a falta de Tecnologia Assistiva e ambientes interativos acessíveis síncronos com recursos de áudio para suporte em EAD principalmente no que tange às pessoas cegas. Assim, o EVOC teve a proposta básica de ser uma ferramenta de interação social síncrona com áudio acessível, implementada, conforme padrões de acessibilidade da W3C/WAI, modelada conforme padrões UML. Distribuído sob licença GPL (software livre), usando o gerenciador de banco de dados MySQL que consiste em uma aplicação desenvolvida na linguagem PHP e FlashCom Server MX. O trabalho também investigou como ocorre o processo de interação entre sujeitos cegos e videntes como viabilidade de inclusão digital através da ferramenta. Posteriormente foram analisados os registros de áudio dos chats transcritos das sessões realizadas.; The article presents an alternative way to achieve digital inclusion, social and education of the blind through (ICT's) in virtual environments for learning, allowing different channels of social interaction. One of the factors...

‣ An integrative analysis of TFBS-clustered regions reveals new transcriptional regulation models on the accessible chromatin landscape

Chen, Hebing; Li, Hao; Liu, Feng; Zheng, Xiaofei; Wang, Shengqi; Bo, Xiaochen; Shu, Wenjie
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Publicado em 16/02/2015 Português
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DNase I hypersensitive sites (DHSs) define the accessible chromatin landscape and have revolutionised the discovery of distinct cis-regulatory elements in diverse organisms. Here, we report the first comprehensive map of human transcription factor binding site (TFBS)-clustered regions using Gaussian kernel density estimation based on genome-wide mapping of the TFBSs in 133 human cell and tissue types. Approximately 1.6 million distinct TFBS-clustered regions, collectively spanning 27.7% of the human genome, were discovered. The TFBS complexity assigned to each TFBS-clustered region was highly correlated with genomic location, cell selectivity, evolutionary conservation, sequence features, and functional roles. An integrative analysis of these regions using ENCODE data revealed transcription factor occupancy, transcriptional activity, histone modification, DNA methylation, and chromatin structures that varied based on TFBS complexity. Furthermore, we found that we could recreate lineage-branching relationships by simple clustering of the TFBS-clustered regions from terminally differentiated cells. Based on these findings, a model of transcriptional regulation determined by TFBS complexity is proposed.

‣ Improved methods for structure probing in large RNAs: a rapid 'heterologous' sequencing approach is coupled to the direct mapping of nuclease accessible sites. Application to the 5' terminal domain of eukaryotic 28S rRNA.

Qu, H L; Michot, B; Bachellerie, J P
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 10/09/1983 Português
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We have developed a combined approach for probing native structures in large RNAs. In the first method, after digestion with a structure specific nuclease, accessible sites are mapped at sequence resolution along the entire RNA molecule which is used as a template for the reverse transcriptase elongation of a 5' end labelled selected primer (coding strand of a small restriction fragment of the cloned gene). This method circumvents any prior end-labelling of RNA, a technique with major limitations for large RNAs. In the second approach, a rapid "heterologous" sequencing can be easily applied to definite domains of an RNA molecule in a variety of species (or individuals), without additional DNA cloning nor end-labelling of RNA. By taking advantage of the presence of evolutionary conserved tracts within an RNA sequence, it allows a rapid analysis of RNA folding patterns in terms of phylogenetic comparisons : when located within such a conserved tract, selected restriction fragments from a cloned gene can be used as heterologous primers for sequencing the upstream divergent region in RNAs of other species by currently available technology, i.e. reverse transcriptase elongation in the presence of chain terminator dideoxynucleotides.

‣ Human membrane transporter database: A web-accessible relational database for drug transport studies and pharmacogenomics

Yan, Qing; Sadée, Wolfgang
Fonte: Springer-Verlag Publicador: Springer-Verlag
Tipo: Artigo de Revista Científica
Publicado em 14/07/2000 Português
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The human genome contains numerous genes that encode membrane transporters and related proteins. For drug discovery, development, and targeting, one needs to know which transporters play a role in drug disposition and effects. Moreover, genetic polymorphisms in human membrane transporters may contribute to interindividual differences in the response to drugs. Pharmacogenetics, and, on a genome-wide basis, pharmacogenomics, address the effect of genetic variants on an individual's response to drugs and xenobiotics. However, our knowledge of the relevant transporters is limited at present. To facilitate the study of drug transporters on a broad scale, including the use of microarray technology, we have constructed a human membrane transporter database (HMTD). Even though it is still largely incomplete, the database contains information on more than 250 human membrane transporters, such as sequence, gene family, structure, function, substrate, tissue distribution, and genetic disorders associated with transporter polymorphisms. Readers are invited to submit additional data. Implemented as a relational database, HMTD supports complex biological queries. Accessible through a Web browser user interface via Common Gateway Interface (CGI) and Java Database Connection (JDBC) (http://128.218.208.23/transporter/trans.html),

‣ Logistic regression models to predict solvent accessible residues using sequence- and homology-based qualitative and quantitative descriptors applied to a domain-complete X-ray structure learning set

Nepal, Reecha; Spencer, Joanna; Bhogal, Guneet; Nedunuri, Amulya; Poelman, Thomas; Kamath, Thejas; Chung, Edwin; Kantardjieff, Katherine; Gottlieb, Andrea; Lustig, Brooke
Fonte: International Union of Crystallography Publicador: International Union of Crystallography
Tipo: Artigo de Revista Científica
Publicado em 10/11/2015 Português
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This article describes the development, validation and application of simple logistic regression models for the prediction of solvent accessible residues in oligomer and non-oligomer sets using a domain-complete X-ray structure learning set.

‣ Multilingual ontology matching based on Wiktionary data accessible via SPARQL endpoint

Lin, Feiyu; Krizhanovsky, Andrew
Fonte: Universidade Cornell Publicador: Universidade Cornell
Tipo: Artigo de Revista Científica
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Interoperability is a feature required by the Semantic Web. It is provided by the ontology matching methods and algorithms. But now ontologies are presented not only in English, but in other languages as well. It is important to use an automatic translation for obtaining correct matching pairs in multilingual ontology matching. The translation into many languages could be based on the Google Translate API, the Wiktionary database, etc. From the point of view of the balance of presence of many languages, of manually crafted translations, of a huge size of a dictionary, the most promising resource is the Wiktionary. It is a collaborative project working on the same principles as the Wikipedia. The parser of the Wiktionary was developed and the machine-readable dictionary was designed. The data of the machine-readable Wiktionary are stored in a relational database, but with the help of D2R server the database is presented as an RDF store. Thus, it is possible to get lexicographic information (definitions, translations, synonyms) from web service using SPARQL requests. In the case study, the problem entity is a task of multilingual ontology matching based on Wiktionary data accessible via SPARQL endpoint. Ontology matching results obtained using Wiktionary were compared with results based on Google Translate API.; Comment: 8 pages...