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‣ Efeito do IFN-k e TNF-α sobre a expressão gênica de CYBB e processamento de seus transcritos.; The effect of IFN-g and TNF-α on CYBB gene expression and its transcripts processing.

Frazão, Josias Brito
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 19/03/2014 Português
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O sistema NADPH oxidase humano é responsável pela geração de reativos intermediários do oxigênio e defeitos neste sistema resultam na Doença Granulomatosa Crônica (DGC). Nesta tese de doutorado, investigamos o efeito do IFN-g sobre eventos pós-transcricionais em pacientes com DGC ligada ao X, ocasionada por defeitos de splicing. Os dados obtidos sugerem que o uso do IFN-g in vitro interfere no processamento da mensagem causando aumento da expressão de transcritos do gene CYBB e NCF1 em células B-EBV de indivíduos sadios e pacientes DGC analisados. Observamos também que o IFN-g dimunui a expressão dos genes THOC4 NONO, SF3A1, SRRM1 e UPF3A e promove aumento de expressão de SRSF10, SNRPA1 e C2 em células B-EBV de paciente X-DGC secundária a defeitos de splicing. Identificamos que o IFN-g e o TNF-α aumentam a expressão das proteínas envolvidas no processo do splicing. Concluímos que o IFN-g aumenta a expressão de genes importantes para uma resposta eficiente do sistema imunológico, incluindo os do sistema NADPH oxidase, além de promover aumento da expressão de genes e de proteínas relacionados ao processo de splicing, que podem estar relacionados aos efeitos benéficos observados no uso do IFN-g em pacientes com DGC ligada ao X...

‣ O perfil do cliente de cartões de crédito, na agência do Sátão da CGD

Coelho, Pedro; Pereira, Paulo Almeida
Fonte: Univerdidade Católica Portuguesa. Instituto Universitário de Desenvolvimento e Promoção Social Publicador: Univerdidade Católica Portuguesa. Instituto Universitário de Desenvolvimento e Promoção Social
Tipo: Artigo de Revista Científica
Publicado em //2001 Português
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Em consequência de um estágio curricular desenvolvido na agência do Sátão da Caixa Geral de Depósitos (CGD), foram recolhidas informações relevantes sobre toda a população de clientes detentores de cartões de crédito, que permitem a sua caracterização, recorrendo a técnicas de análise estatística. Com o objectivo de verificar as diferenças entre os clientes dos dois segmentos deste produto, cartão “Classic” e “Gold”, este com maior prestígio, aplicam-se técnicas de estatística descritiva para salientar as desigualdades entre os clientes dos dois produtos. O método de regressão logística múltipla é utilizado para a definição do perfil dos clientes, permitindo determinar as variáveis relevantes para a atribuição desses cartões, bem como a importância relativa, de cada uma dessas variáveis significativas, para a segmentação dos clientes. O modelo também permite a previsão do tipo de cartão de cada cliente, a partir das suas características, e respectiva comparação com o cartão que efectivamente possui.

‣ O perfil do cliente de Fundos de Investimento Mobiliário Caixagest, na agência de Viseu da CGD

Rodrigues, Sandra; Pereira, Paulo Almeida
Fonte: Universidade Católica Portuguesa. Instituto Universitário de Desenvolvimento e Promoção Social Publicador: Universidade Católica Portuguesa. Instituto Universitário de Desenvolvimento e Promoção Social
Tipo: Artigo de Revista Científica
Publicado em //2000 Português
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Em consequência de um estágio curricular desenvolvido na agência de Viseu da Caixa Geral de Depósitos (CGD), foram recolhidas informações relevantes sobre toda a população de clientes com aplicações em Fundos de Investimento Mobiliário Caixagest. Este trabalho pretende apresentar os vários fundos existentes, bem como o perfil do cliente Caixagest da agência bancária em análise. São estudados todos os clientes (particulares e pessoas colectivas) deste tipo de aplicações, utilizando métodos de estatística descritiva, sendo analisadas algumas das variáveis que os definem, como por exemplo: género, idade, estado civil, local de residência, actividade profissional, se são emigrantes ou reformados. Estas variáveis são posteriormente cruzadas com os montantes totais e valores médios investidos em cada tipo de fundos, sendo ainda aplicada a técnica de análise factorial de componentes principais aos montantes aplicados por todos os clientes particulares nos vários fundos. Consegue, assim, definir-se o perfil do cliente de fundos de investimento imobiliário Caixagest na agência de Viseu da CGD.

‣ A comunicação interna e o voluntariado corporativo: estudo de caso no setor bancário - CGD

Pimentel, Catarina de Melo Vilela
Fonte: Instituto Superior de Ciências Sociais e políticas Publicador: Instituto Superior de Ciências Sociais e políticas
Tipo: Dissertação de Mestrado
Publicado em 15/07/2013 Português
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Tese de Mestrado em Comunicação Social, vertente Comunicação Estratégica; O presente trabalho insere-se no mestrado em comunicação social, vertente Comunicação Estratégica e teve como objetivo a análise das práticas, propostas e sugestões para ações de voluntariado e de comunicação interna (CI) no setor bancário, mais precisamente na Caixa Geral de Depósitos (CGD), onde foi realizado um estágio nas áreas de comunicação e voluntariado. O principal objetivo do presente relatório foi analisar e responder à seguinte questão: “Como pode a comunicação interna promover a responsabilidade social, designadamente o voluntariado corporativo, no setor bancário?” Para tal, em termos de método de investigação, optou-se por uma metodologia mista de recolha de dados: qualitativa e quantitativa. As principais técnicas utilizadas foram inquéritos por entrevista e por questionário, e observação participante. Após a análise do caso em estudo, conclui-se que a CI na CGD é fundamental para o sucesso do negócio. A CI pode ser vista como forma de promoção e de divulgação para ações de voluntariado corporativo. Conclui-se também que o mais importante não será a implementação de mais ações de voluntariado na CGD...

‣ A internacionalização bancária – destino Brasil: o caso CGD

Ribeiro, Clara Maria Silva
Fonte: Instituto Universitário de Lisboa Publicador: Instituto Universitário de Lisboa
Tipo: Dissertação de Mestrado
Publicado em //2011 Português
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Mestrado em Finanças; Este documento teve como objectivo analisar o processo de internacionalização, da Caixa Geral de Depósitos (CGD) para o Brasil. Concretizamos a tese através de um estudo qualitativo, suportado por um referencial teórico. A revisão da literatura centrou-se na Teoria Eclética e na Teoria da Internalização. O estudo em causa analisa o processo de internacionalização de um banco, sugerindo que esse processo é levado a cabo quando se pretendem reunir sinergias com os seus clientes e adquirir valor para os seus stakeholders. Procedemos a uma análise do sector financeiro brasileiro, com a respectiva caracterização e evolução histórica, enquadrada num contexto macroeconómico. Através deste estudo podemos sugerir que o país de destino da internacionalização tende a ter motivações históricas e/ou culturais. Observamos que existe ausência de informação sobre um processo histórico e relevante, quer para a CGD, quer para Portugal e para o Brasil, no que diz respeito à internacionalização da CGD para o Brasil. Finalmente, procuramos enquadrar a importância estratégica da presença da CGD no Brasil e concluir que a dimensão dos movimentos empresariais de, e para o Brasil, está expressa no reinício do investimento que o Grupo decidiu realizar no país...

‣ Environmental sustainability as a dimension of corporate social responsibility: the case of CGD - Caixa Geral de Depósitos / Portugal

Coelho, M. P.; Filipe, J. A.; Ferreira, Manuel Alberto M.
Fonte: Progress IPS LLC Publicador: Progress IPS LLC
Tipo: Artigo de Revista Científica
Publicado em //2011 Português
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According to the 2001 European Commission Green Paper, Social Responsibility conceptualization is associated with the idea of companies deciding, in a voluntary base, to contribute for a fair society and a cleaner environment. The tradition of social intervention in Portugal remounts to the century XV and the foundation of the Mercies. However, environmental goals were never proposed as fundamental objectives in the positioning of corporate social responsibility, given the delay of the industrial development. In spite of the tradition of practice, in informal way, of social protection by the companies of larger dimension, the appearance of “social responsibility” as an autonomous management theme, took, to some enterprises, the interest in environmental subjects, crossing a paternalist vision that put the accent in the relationships with the workers. The CGD is an example of this posture. In 2009, adhered to the Environmental Program of the United Nations for the Finance Sector (UNEP FI) becoming the first Portuguese bank to do part of this program. UNEP FI reflects the recognition of the role of this sector in the promotion of Sustainable Development: market intermediation processes have effects on investment programs and, in indirect way...

‣ Expression of the X-CGD gene during induced differentiation of myeloid leukemia cell line HL-60.

Barker, K A; Orkin, S H; Newburger, P E
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /07/1988 Português
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The expression of the X-CGD gene, which encodes the heavy-chain subunit of the phagocyte cytochrome b, was studied during induced myeloid differentiation of HL-60 cells. Incubation of the cells with a combined regimen of retinoic acid and dimethyl formamide resulted in granulocytic morphological differentiation and acquisition of nitroblue tetrazolium reduction, a measure of superoxide generation. During the 5-day course of induced differentiation, the levels of X-CGD mRNA transcripts rose 13-fold, with a 2-fold increase detectable within 3 h of exposure to retinoic acid. Relative transcription rates for the X-CGD gene, determined by nuclear runoff, increased two- to eightfold after 24 to 72 h of induced differentiation. However, the greater change in X-CGD mRNA levels than that in transcription rates implies the involvement of posttranscriptional regulation as well. Fractionation by centrifugal elutriation into phases of the cell cycle showed expression of X-CGD transcripts predominantly in G1 cells before induction and in all phases of the cell cycle 24 h after induction. Thus the rapid increase in X-CGD expression in induced cells reflects the acquisition of functional competence and not the concomitant cessation of proliferation or shift in cell cycle distribution.

‣ The Candida Genome Database (CGD), a community resource for Candida albicans gene and protein information

Arnaud, Martha B.; Costanzo, Maria C.; Skrzypek, Marek S.; Binkley, Gail; Lane, Christopher; Miyasato, Stuart R.; Sherlock, Gavin
Fonte: Oxford University Press Publicador: Oxford University Press
Tipo: Artigo de Revista Científica
Português
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The Candida Genome Database (CGD) is a new database that contains genomic information about the opportunistic fungal pathogen Candida albicans. CGD is a public resource for the research community that is interested in the molecular biology of this fungus. CGD curators are in the process of combing the scientific literature to collect all C.albicans gene names and aliases; to assign gene ontology terms that describe the molecular function, biological process, and subcellular localization of each gene product; to annotate mutant phenotypes; and to summarize the function and biological context of each gene product in free-text description lines. CGD also provides community resources, including a reservation system for gene names and a colleague registry through which Candida researchers can share contact information and research interests. CGD is publicly funded (by NIH grant R01 DE15873-01 from the NIDCR) and is freely available at http://www.candidagenome.org/.

‣ Processing and presentation of tetanus toxin by antigen-presenting cells from patients with chronic granulomatous disease (CGD) to human specific T cell clones are not impaired.

Barbey, C; Tiercy, J M; Fairweather, N; Niemann, H; Seger, R; Corradin, G
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /02/1994 Português
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The capacity of peripheral blood lymphocytes (PBL) or Epstein-Barr virus (EBV)-transformed B cell lines from CGD patients to process and present tetanus toxin (tt)-specific epitopes was assessed using various tt preparations and human tt-specific T cell clones. PBL from all of the donors were able to process and present either native tt and/or denatured tt to human T cell clones specific for various tt epitopes. Furthermore, no difference was found in the antigen requirement when normal or CGD EBV-B cell lines were used as antigen-presenting cells (APC). These results suggest that the deficiency in oxygen metabolism in CGD cells does not affect tt processing and presentation.

‣ Restitution of superoxide generation in autosomal cytochrome-negative chronic granulomatous disease (A22(0) CGD)-derived B lymphocyte cell lines by transfection with p22phax cDNA

Fonte: The Rockefeller University Press Publicador: The Rockefeller University Press
Tipo: Artigo de Revista Científica
Publicado em 01/12/1993 Português
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The respiratory burst oxidase of phagocytes and B lymphocytes is a multicomponent enzyme that catalyzes the one-electron reduction of oxygen by NADPH. It is responsible for the O2-production that occurs when these cells are exposed to phorbol 12-myristate 13-acetate or physiologic stimuli, such as phagocytosis in phagocytes or cross- linking of surface immunoglobulin in B lymphocytes. The activity of this enzyme is greatly diminished or absent in patients with chronic granulomatous disease (CGD), an inherited disorder characterized by a severe defect in host defense against bacteria and fungi. In every CGD patient studied so far, an abnormality has been found in a gene encoding one of the four components of the respiratory burst oxidase: the membrane proteins p22phox or gp91phox which together form the cytochrome b558 protein, or the cytosolic proteins p47phox or p67phox. Autosomal recessive cytochrome-negative CGD (A22(0) CGD) is associated with mutations in the gene coding for p22phox. We report here that the capacity for O2- production and cytochrome b558 protein expression were restored to Epstein-Barr virus-transformed B lymphocytes from two A22(0) CGD patients by transfection with an expression plasmid containing a p22phox cDNA. No detectable O2- was generated by untransfected p22phox-deficient lymphocytes. The genetic reconstitution of the respiratory burst in A22(0) CGD B lymphocytes by transfer of the wild-type p22phox cDNA represents a further step towards somatic gene therapy for this subgroup of A22(0) CGD. This system will also be useful for expression of genetically engineered mutant p22phox proteins in intact cells...

‣ Impaired apoptotic cell clearance in CGD due to altered macrophage programming is reversed by phosphatidylserine-dependent production of IL-4

Fernandez-Boyanapalli, Ruby F.; Frasch, S. Courtney; McPhillips, Kathleen; Vandivier, R. William; Harry, Brian L.; Riches, David W. H.; Henson, Peter M.; Bratton, Donna L.
Fonte: American Society of Hematology Publicador: American Society of Hematology
Tipo: Artigo de Revista Científica
Publicado em 26/02/2009 Português
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Chronic granulomatous disease (CGD) is characterized by overexuberant inflammation and autoimmunity that are attributed to deficient anti-inflammatory signaling. Although regulation of these processes is complex, phosphatidylserine (PS)–dependent recognition and removal of apoptotic cells (efferocytosis) by phagocytes are potently anti-inflammatory. Since macrophage phenotype also plays a beneficial role in resolution of inflammation, we hypothesized that impaired efferocytosis in CGD due to macrophage skewing contributes to enhanced inflammation. Here we demonstrate that efferocytosis by macrophages from CGD (gp91phox−/−) mice was suppressed ex vivo and in vivo. Alternative activation with interleukin 4 (IL-4) normalized CGD macrophage efferocytosis, whereas classical activation by lipopolysaccharide (LPS) plus interferon γ (IFNγ) had no effect. Importantly, neutralization of IL-4 in wild-type macrophages reduced macrophage efferocytosis, demonstrating a central role for IL-4. This effect was shown to involve 12/15 lipoxygenase and activation of peroxisome-proliferator activated receptor γ (PPARγ). Finally, injection of PS (whose exposure is lacking on CGD apoptotic neutrophils) in vivo restored IL-4–dependent macrophage reprogramming and efferocytosis via a similar mechanism. Taken together...

‣ Restoration of NET formation by gene therapy in CGD controls aspergillosis

Bianchi, Matteo; Hakkim, Abdul; Brinkmann, Volker; Siler, Ulrich; Seger, Reinhard A.; Zychlinsky, Arturo; Reichenbach, Janine
Fonte: American Society of Hematology Publicador: American Society of Hematology
Tipo: Artigo de Revista Científica
Publicado em 24/09/2009 Português
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Chronic granulomatous disease (CGD) patients have impaired nicotinamide adenine dinucleotide phosphate (NADPH) oxidase function, resulting in poor antimicrobial activity of neutrophils, including the inability to generate neutrophil extracellular traps (NETs). Invasive aspergillosis is the leading cause of death in patients with CGD; it is unclear how neutrophils control Aspergillus species in healthy persons. The aim of this study was to determine whether gene therapy restores NET formation in CGD by complementation of NADPH oxidase function, and whether NETs have antimicrobial activity against Aspergillus nidulans. Here we show that reconstitution of NET formation by gene therapy in a patient with CGD restores neutrophil elimination of A nidulans conidia and hyphae and is associated with rapid cure of preexisting therapy refractory invasive pulmonary aspergillosis, underlining the role of functional NADPH oxidase in NET formation and antifungal activity.

‣ Biochemical Correction of X-CGD by a Novel Chimeric Promoter Regulating High Levels of Transgene Expression in Myeloid Cells

Santilli, Giorgia; Almarza, Elena; Brendel, Christian; Choi, Uimook; Beilin, Chiara; Blundell, Michael P; Haria, Sneha; Parsley, Kathryn L; Kinnon, Christine; Malech, Harry L; Bueren, Juan A; Grez, Manuel; Thrasher, Adrian J
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Português
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X-linked chronic granulomatous disease (X-CGD) is a primary immunodeficiency caused by mutations in the CYBB gene encoding the phagocyte nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase catalytic subunit gp91phox. A recent clinical trial for X-CGD using a spleen focus-forming virus (SFFV)–based γ-retroviral vector has demonstrated clear therapeutic benefits in several patients although complicated by enhancer-mediated mutagenesis and diminution of effectiveness over time due to silencing of the viral long terminal repeat (LTR). To improve safety and efficacy, we have designed a lentiviral vector that directs transgene expression primarily in myeloid cells. To this end, we created a synthetic chimeric promoter that contains binding sites for myeloid transcription factors CAAT box enhancer–binding family proteins (C/EBPs) and PU.1, which are highly expressed during granulocytic differentiation. As predicted, the chimeric promoter regulated higher reporter gene expression in myeloid than in nonmyeloid cells, and in human hematopoietic progenitors upon granulocytic differentiation. In a murine model of stem cell gene therapy for X-CGD, the chimeric vector resulted in high levels of gp91phox expression in committed myeloid cells and granulocytes...

‣ Inflammation and repeated infections in CGD: two sides of a coin

Kuijpers, Taco; Lutter, Rene
Fonte: SP Birkhäuser Verlag Basel Publicador: SP Birkhäuser Verlag Basel
Tipo: Artigo de Revista Científica
Português
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Chronic granulomatous disease (CGD) is an uncommon congenital immunodeficiency seen approximately in 1 of 250,000 individuals. It is caused by a profound defect in a burst of oxygen consumption that normally accompanies phagocytosis in all myeloid cells (neutrophils, eosinophils, monocytes, and macrophages). This “respiratory burst” involves the catalytic conversion of molecular oxygen to the oxygen free-radical superoxide, which in turn gives rise to hydrogen peroxide, hypochlorous acid, and hydroxyl radicals. These oxygen derivatives play a critical role in the killing of pathogenic bacteria and fungi. As a result of the failure to activate the respiratory burst in their phagocytes, the majority of CGD patients suffer from severe recurrent infections and rather unexplained prolonged inflammatory reactions that may result in granulomatous lesions. Both may cause severe organ dysfunction depending on the tissues involved. Preventive measures as well as rapid (invasive) diagnostic procedures are required to successfully treat CGD. Hematopoietic stem cell transplantation may be a serious option in some of the patients.

‣ Alpharetroviral Vector-mediated Gene Therapy for X-CGD: Functional Correction and Lack of Aberrant Splicing

Kaufmann, Kerstin B.; Brendel, Christian; Suerth, Julia D.; Mueller-Kuller, Uta; Chen-Wichmann, Linping; Schwäble, Joachim; Pahujani, Shweta; Kunkel, Hana; Schambach, Axel; Baum, Christopher; Grez, Manuel
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Português
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Comparative integrome analysis has revealed that the most neutral integration pattern among retroviruses is attributed to alpharetroviruses. We chose X-linked chronic granulomatous disease (X-CGD) as model to evaluate the potential of self-inactivating (SIN) alpharetroviral vectors for gene therapy of monogenic diseases. Therefore, we combined the alpharetroviral vector backbone with the elongation factor-1α short promoter, both considered to possess a low genotoxic profile, to drive transgene (gp91phox) expression. Following efficient transduction transgene expression was sustained and provided functional correction of the CGD phenotype in a cell line model at low vector copy number. Further analysis in a murine X-CGD transplantation model revealed gene-marking of bone marrow cells and oxidase positive granulocytes in peripheral blood. Transduction of human X-CGD CD34+ cells provided functional correction up to wild-type levels and long-term expression upon transplantation into a humanized mouse model. In contrast to lentiviral vectors, no aberrantly spliced transcripts containing cellular exons fused to alpharetroviral sequences were found in transduced cells, implying that the safety profile of alpharetroviral vectors may extend beyond their neutral integration profile. Taken together...

‣ Primary cutaneous γδ-T-cell lymphoma (CGD-TCL) with unilateral lower extremity swelling as first-onset symptom: a rare case report

Li, Duo; Huang, Lijun; Guo, Bin; Wen, Qiuyuan; Wang, Weiyuan; Luo, Jiadi; Fan, Songqing
Fonte: e-Century Publishing Corporation Publicador: e-Century Publishing Corporation
Tipo: Artigo de Revista Científica
Publicado em 15/07/2014 Português
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Primary cutaneous γδ-T-cell lymphoma (CGD-TCL) is a distinct disease entity which is an extremely rare neoplasm with poor prognosis, characterized by the γ/δ T-cell receptor expression on atypical lymphocytes. We report the case of a 42-year-old man who first presented with a swelling in the extremities and subsequent appeared subcutaneous nodule over the body. In order to clarify the diagnosis, a biopsy of subcutaneous nodule for pathology had been done. CGD-TCL was diagnosed by histopathology, immunophenotype, in situ hybridization and analysis of TCRγ genes rearrangement. The patient was treated with chemotherapeutic regimens-CHOP (cyclophosphamide, doxorubicin, vincristine and prednisolone). After one period of chemotherapy, subcutaneous nodules became small, even disappeared, swelling and ulcer in the left pedal gone away gradually. One month later after first chemotherapy, tumor relapsed with lesions growing back rapidly, also showed disease in double lungs. The patient was just 10-month survival time from the onset. To our knowledge, this case is the first report of CGD-TCL with unilateral lower extremity swelling as the first-onset symptom. If patient is presented the first symptoms such as swelling of extremities, especially when ulceration appears...

‣ Generation of X-CGD cells for vector evaluation from healthy donor CD34+ HSCs by shRNA-mediated knock down of gp91phox

Brendel, Christian; Kaufmann, Kerstin B; Krattenmacher, Anja; Pahujani, Shweta; Grez, Manuel
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Publicado em 27/08/2014 Português
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Innovative approaches for the treatment of rare inherited diseases are hampered by limited availability of patient derived samples for preclinical research. This also applies for the evaluation of novel vector systems for the gene therapy of monogenic hematological diseases like X-linked chronic granulomatous disease (X-CGD), a severe primary immunodeficiency caused by mutations in the gp91phox subunit of the phagocytic NADPH oxidase. Since current gene therapy protocols involve ex vivo gene modification of autologous CD34+ hematopoietic stem cells (HSC), the ideal preclinical model should simulate faithfully this procedure. However, the low availability of patient-derived CD34+ cells limits the feasibility of this approach. Here, we describe a straightforward experimental strategy that circumvents this limitation. The knock down of gp91phox expression upon lentiviral delivery of shRNAs into CD34+ cells from healthy donors generates sufficient amounts of X-CGD CD34+ cells which subsequently can be used for the evaluation of novel gene therapeutic strategies using a codon-optimized gp91phox transgene. We have used this strategy to test the potential of a novel gene therapy vector for X-CGD.

‣ Vergleichende Untersuchungen zur Wirkung verschiedener Liposomenformulierungen auf das humane und murine Komplementsystem; Comparing studies of effectiveness of different liposomes on the human and murine complement system

Bauer, Daniela
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
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Der vererbte Defekt der NADPH-Oxidase phagozytärer Zellen hat die Septische Granulomatose (Chronic Granulomatous Disease, CGD), ein schweres Krankheitsbild mit rezidivierenden Infektionen und meist schlechter Prognose, zur Folge. Eine Hypothese ist, dass der vorliegende Mangel des intakten Enzyms NADPH-Oxidase durch spezifische Aufnahme von Glucose-Oxidase-haltigen Liposomen (GOL) durch die Zellen des Phagozytosesystems ausgeglichen werden kann, womit eine alternative Therapiemöglichkeit der CGD durch intravenöse Verabreichung von GOL bestünde. Im Rahmen dieser Arbeit wurde die Aktivierung des murinen und humanen Komplementsystems durch verschiedene Liposomenformulierungen (Eiphosphatidylcholin- (EPC), Eiphosphatidylglycerol- (EPG) und Polyethylenglykol- (PEG) Liposomen, im murinen System zusätzlich durch Glucose-Oxidase-haltige Liposomen) mittels diverser in-vitro-Modelle als auch in vivo in einem Mausmodell für CGD, z.T. nach Infektion mit verschiedenen krankheitstypischen Erregern untersucht. Des Weiteren wurden die murinen Modelle mit dem humanen System verglichen. In unseren Versuchen zeigte sich insgesamt eine Aktivierung des Komplementsystems durch die verschiedenen Liposomenformulierungen. Sowohl im murinen als auch im humanen System gab es bei einzelnen Liposomenformulierungen eine unterschiedlich starke Auswirkung auf die verschiedenen untersuchten Komponenten des Komplementsystems. Im Ganzen zeigte sich im murinen und im humanen System eine geringere Aktivierung des Komplements durch PEG-Liposomen als durch EPC- und EPG-Liposomen...

‣ A contabilidade ambiental no sector financeiro: a CGD e o programa Caixacarbono Zero

Calado, Rui Sérgio Moreira
Fonte: Instituto Universitário de Lisboa Publicador: Instituto Universitário de Lisboa
Tipo: Dissertação de Mestrado
Publicado em //2011 Português
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Mestrado em Contabilidade; Este projecto pretende abordar a temática da Contabilidade Ambiental. O estudo incide sobre uma organização do sector financeiro – a Caixa Geral de Depósitos, S.A. (CGD), e em particular o seu Programa Caixa Carbono Zero, mormente no que diz respeito à instalação de painéis solares (fotovoltaicos), no seu edifício sede. A informação é analisada com base no ano de 2009, dado ser a informação completa disponível durante a elaboração deste projecto académico. A CGD é um dos maiores bancos do sistema financeiro português, detido a 100% pelo Estado Português. Tem cerca de 11.000 funcionários que laboram não só em Portugal, mas também no estrangeiro, em países onde esta organização tem interesses estratégicos. Estes interesses encontram-se materializados em Bancos, Sucursais, Escritórios de Representação, entre outras participações. Iniciamos o trabalho tendo em vista identificar o contexto em que se insere a Contabilidade Ambiental, nomeadamente através da definição do problema e o seu contexto. De seguida, apresenta-se uma revisão de literatura, onde é pretendido abordar a perspectiva histórica e conceitos de Contabilidade Ambiental e perceber a Sustentabilidade nas organizações e o seu relato. No capítulo 3 são apresentados os objectivos do projecto...

‣ Fas and FasL expression in leukocytes from Chronic Granulomatous Disease patients

Montes-Berrueta,Daniela; Ramírez,Lorena; Salmen,Siham; Berrueta,Lisbeth
Fonte: Universidad del Zulia Publicador: Universidad del Zulia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2012 Português
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Chronic Granulomatous Disease (CGD) is a primary immunodeficiency characterized by defects in superoxide (O2-) production, which result from mutations in one of the four NADPH oxidase components, predisposing to bacterial and fungal infections. Besides the O2-defect, it has been described that neutrophils from CGD patients are resistant to cell death, a phenomenon that has been connected to chronic inflammation and predisposition to autoimmune diseases. A diminished expression of Fas and its counterpart FasL, molecules known to play a major role in cell death, has been described in lymphocytes depleted of O2-reactive oxygen species (ROS), suggesting an involvement of ROS in Fas/FasL expression. In this work, Fas and FasL expressions were analyzed in T cells and neutrophils from two CGD families, previously known to harbor two different molecular defects: absence of either p47-phox or p67-phox. We found that T lymphocytes from CGD patients express low levels of Fas and FasL, while a diminished FasL expression was observed on neutrophils from a CGD A470 patient. These defects may contribute to understand altered cell death in CGD patients