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‣ Impact of cytomegalovirus and grafts versus host disease on the dynamics of CD57+CD28-CD8+ T cells after bone marrow transplant

MENDES, Ana Verena Almeida; KALLAS, Esper Georges; BENARD, Gil; PANNUTI, Cláudio Sérgio; MENEZES, Reneé; DULLEY, Frederico Luiz; EVANS, Thomas George; SALOMÃO, Reinaldo; MACHADO, Clarisse Martins
Fonte: Faculdade de Medicina / USP Publicador: Faculdade de Medicina / USP
Tipo: Artigo de Revista Científica
Português
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OBJECTIVES: The present study aimed to evaluate the dynamics of CD28 and CD57 expression in CD8+ T lymphocytes during cytomegalovirus viremia in bone marrow transplant recipients. METHODS: In a prospective study, blood samples were obtained once weekly once from 33 healthy volunteers and weekly from 33 patients. To evaluate the expression of CD57 and CD28 on CD8+ T lymphocytes, flow cytometry analysis was performed on blood samples for four months after bone marrow transplant, together with cytomegalovirus antigenemia assays. RESULTS: Compared to cytomegalovirus-seronegative healthy subjects, seropositive healthy subjects demonstrated a higher percentage of CD57+ and a lower percentage of CD28+ cells (p<0.05). A linear regression model demonstrated a continuous decrease in CD28+ expression and a continuous increase in CD57+ expression after bone marrow transplant. The occurrence of cytomegalovirus antigenemia was associated with a steep drop in the percentage of CD28+ cells (5.94%, p<0.01) and an increase in CD57+ lymphocytes (5.60%, p<0.01). This cytomegalovirus-dependent effect was for the most part concentrated in the allogeneic bone marrow transplant patients. The development of acute graft versus host disease, which occurred at an earlier time than antigenemia (day 26 vs. day 56 post- bone marrow transplant)...

‣ Human cytomegalovirus reinfection is associated with intrauterine transmission in a highly cytomegalovirus-immune maternal population

YAMAMOTO, Aparecida Yulie; MUSSI-PINHATA, Marisa Marcia; BOPPANA, Suresh B.; NOVAK, Zdenek; WAGATSUMA, Virginia M.; OLIVEIRA, Patricia de Frizzo; DUARTE, Geraldo; BRITT, William J.
Fonte: MOSBY-ELSEVIER Publicador: MOSBY-ELSEVIER
Tipo: Artigo de Revista Científica
Português
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OBJECTIVE: To determine contribution of reinfection with new strains of cytomegalovirus in cytomegalovirus seromimmune women to incidence of congenital cytomegalovirus infection. STUDY DESIGN: In 7848 women studied prospectively for congenital cytomegalovirus infection from a population with near universal cytomegalovirus seroimmunity, sera from 40 mothers of congenitally infected infants and 109 mothers of uninfected newborns were analyzed for strain-specific anticytomegalovirus antibodies. RESULTS: All women were cytomegalovirus seroimmune at first prenatal visit. Reactivity for 2 cytomegalovirus strains was found in 14 of 40 study mothers and in 17 of 109 control mothers at first prenatal visit (P=.009). Seven of 40 (17.5%) study women and 5 of 109 (4.6%) controls (P=.002) acquired antibodies reactive with new cytomegalovirus strains during pregnancy. Evidence of infection with more than 1 strain of cytomegalovirus before or during current pregnancy occurred in 21 of 40 study mothers and 22 of 109 controls (P<.0001). CONCLUSION: Maternal reinfection by new strains of cytomegalovirus is a major source of congenital infection in this population.; National Institutes of Health (NIH)[NIAID AI 49537]; National Institutes of Health (NIH)[R03 TW006480]; National Institutes of Health (NIH)[NIDCD DC04162]; Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)...

‣ Tumor necrosis factor region polymorphisms are associated with AIDS and with cytomegalovirus retinitis

DEGHAIDE, Neifi H. S.; RODRIGUES, Maria de L. V.; CASTELLI, Erick C.; MENDES-JUNIOR, Celso T.; FIGUEIREDO, Jose F. C.; DONADI, Eduardo A.
Fonte: LIPPINCOTT WILLIAMS & WILKINS Publicador: LIPPINCOTT WILLIAMS & WILKINS
Tipo: Artigo de Revista Científica
Português
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Background: The tumor necrosis factor (TNF) gene is located within the highly polymorphic major histocompatibility complex region, exhibiting the -308 CA promoter region polymorphism and six microsatellites (TNFa-f) spanning the region nearby the TNF locus. Objective: In the present Study, we evaluated the frequency of -308 CA and TNFa-e polymorphisms and respective haplotypes (in chromosomal sequence: TNFd-TNFe-308GA-TNFc-TNFa-TNFb), in 222 patients with AIDS, 52 of whom exhibited cytomegalovirus retinitis, and in 202 healthy HIV-negative individuals. Method: TNF microsatellite and single nucleotide polymorphism typings were performed by PCR followed by polyacrylamide gel electrophoresis. Results: The TNF-308A allele and the 4-3-C-2-7-1 haplotype were associated with susceptibility to AIDS, whereas the TNFb4 allele and the 3-3-C-1-11-4 haplotype were associated with protection against AIDS development. The TNFc2 allele and the 4-1-G-2-2-1 haplotype, which contains the TNFc2 allele, were associated with cytomegalovirus retinitis. Conclusion: This study highlights that polymorphic sites spanning the region nearby the TNF locus are associated with AIDS per se and with cytomegalovirus retinitis in AIDS patients. (C) 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins

‣ Prevalência de infecção congênita por citomegalovírus em recém-nascidos de uma unidade de tratamento intensivo de um hospital público; The prevalence of congenital cytomegalovirus infection in newborn infants at an intensive care unit in a public hospital

Miura, Clarissa Schreiner; Miura, Ernani; Machado, Alice Beatriz Mombach Pinheiro; Chesky, Marisa
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
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Objetivo: Determinar a prevalência da infecção congênita por citomegalovírus em recém-nascidos internados na unidade de terapia intensiva neonatal de um hospital público de Porto Alegre. Métodos: Estudo transversal, incluindo 261 recém-nascidos que nasceram em um hospital público da cidade de Porto Alegre no ano de 2003 e foram internados na unidade de terapia intensiva neonatal. Foi coletada amostra de urina nos primeiros 7 dias de vida e realizado o teste de reação em cadeia da polimerase para a pesquisa do DNA do citomegalovírus. Resultados: A prevalência de infecção congênita por citomegalov írus na população estudada foi de 0,8% (IC 95%: 0,097-2,86). Devido à baixa prevalência, não foi possível associar fatores de risco. Conclusões: A prevalência de infecção congênita por citomegalov írus em uma unidade de terapia intensiva neonatal de um hospital público de Porto Alegre não foi considerada elevada, sendo semelhante à prevalência encontrada em outros estudos realizados.; Objective: To determine the prevalence of congenital cytomegalovirus infection in newborn infants admitted to an intensive care unit in a public hospital in Porto Alegre. Methods: A cross-sectional study of 261 newborn infants born at a public hospital in the city of Porto Alegre in 2003 and admitted to the intensive care ward. Urine samples were collected within 7 days of birth and a polymerase chain reaction-PCR performed to test for cytomegalovirus DNA. Results: The prevalence of congenital cytomegalovirus infection among the study population was 0.8% (95% CI: 0.097-2.86). It was not possible to assess risk factors because this prevalence was so low. Conclusions: The prevalence of congenital cytomegalovirus infection in an intensive care unit at a public hospital in Porto Alegre was not considered elevated and was comparable with prevalence rates found by other studies.

‣ Cytomegalovirus transmission in breast milk : integrative review; Transmissão do citomegalovírus pelo leite materno : revisión integrativa; Transmisión del citomegalovírus por la leche materna : revisión integrativa

Oliveira, Gustavo Costa de; Gonçalves, Annelise de Carvalho; Ferreira, Gimerson Erick; Lima, Beatriz Santana de Souza; Rocha, Luanna dos Santos; Strapasson, Márcia Rejane
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
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Objetivo: avaliar as evidências da transmissão do citomegalovírus ao recém-nascido pelo leite materno e suas repercussões. Método: revisão integrativa com vistas a responder à questão << Como a transmissão do citomegalovírus ao recém-nascido através do leite materno está sendo abordada em publicações científicas nacionais e internacionais? Para isso, foram consultadas as bases de dados LILACS, Web of Science, BDENF e biblioteca virtual SciELO. Resultados: a amostra final foi composta por nove artigos que descreveram medidas preventivas como pasteurização, resfriamento, congelamento, e utilização de Ganciclovir pelo neonato; os bebês prematuros apresentaram sepse e doenças respiratórias, após a contaminação por citomegalovírus pelo leite materno, em diferentes taxas de infecção. Conclusão: evidenciaram-se discordâncias nos achados, especialmente relacionadas a dados epidemiológicos da transmissibilidade pelo leite materno e a forma de oferecimento dessa substância aos recém-nascidos, sendo indispensável estimular produções científicas nessa temática no intuito de potencializar maior difusão desse conhecimento e possibilitar novas estratégias de intervenção.; Objective: to evaluate the evidence of Cytomegalovirus transmission to the newborn through breast milk and its repercussions. Method: integrative review in order to answer the question << How the transmission Cytomegalovirus to the newborn via breast milk is being addressed in national and international scientific publications? >> For this purpose...

‣ PP65 antigenemia in the diagnosis of cytomegalovirus infection in AIDS patients

Capela, R. C.; Grassi, A.; Souza, L. R.
Fonte: Universidade Estadual Paulista (UNESP), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) Publicador: Universidade Estadual Paulista (UNESP), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
Tipo: Artigo de Revista Científica Formato: 103-108
Português
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 0312374-0; Cytomegalovirus causes significant morbidity and mortality in AIDS patients and those having undergone bone marrow or another transplant. PP65 antigenemia is based on detecting viral antigen in peripheral blood leukocytes through immunochemistry and by monitoring the infection in immunocompromised individuals. The present study aimed to set up this diagnostic technique in AIDS patients with active cytomegalovirus infection and verify its occurrence in the Botucatu region of São Paulo state, Brazil. Fifty patients, 35 men and 15 women aged from 24 to 69 years, were recruited from those attended at the Department of Tropical Diseases of Botucatu Medical School, UNESP, and divided into three groups according to CD4+ T lymphocyte counts and antiretroviral treatment. The control group comprised bone marrow transplant patients. Fourteen AIDS patients with low CD4+ cell counts tested positive for PP65 antigenemia, which could predict cytomegalovirus infection and indicate prophylactic treatment.

‣ Gastrointestinal cytomegalovirus disease in renal transplant recipients: A case series

de Andrade, Luís G.M.; Rodrigues, Maria A.M.; Romeiro, Fernando G.; Carvalho, Maria F.C.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 345-350
Português
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The purpose of this article was to report a series of 23 renal transplant recipients with histologically proven and immunohistochemically confirmed cytomegalovirus (CMV) lesions in the gastrointestinal tract (GIT) and to assess the risk factors associated with severe disease/mortality. CMV patients (n=23) were allocated into two groups: those who died (n=6) and those considered cured (n=17). Overall mortality rate was 26% (6/23). Initial symptoms suggestive of lower GIT involvement were observed in all death cases and in 35.3% of those cured (p=0.01). Enterorrhagia was seen in 83.3% of the patients who died. Death risk increased twofold (RR 2 [1.13-3.52], p=0.01) when symptoms of lower GIT involvement were initially observed and sixfold when enterrohagia was present (RR 6 [1.1-35.9], p=0.001). Among death cases, mean time at diagnosis was significantly more distant (2002±2.9×2008±1.6, p=0.04). The difference in mortality rates seen as service practices changed along the years demonstrates the importance of early diagnosis. © 2011 John Wiley & Sons A/S.

‣ Citomegalovírus, herpesvírus humano 6, herpesvírus humano 7 e perfil imunofenotípico do infiltrado inflamatório na periodontite crônica marginal; Cytomegalovirus, human herpesvirus 6, human herpesvirus 7 and immunophenotypic profile of inflammatory infiltrate in marginal chronic periodontitis

Ronaldo Luís Thomasini
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 04/11/2011 Português
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Periodontite humana crônica é um processo inflamatório caracterizado por denso acúmulo de células imunes no tecido periodontal. A periodontite pode levar a perda do dente no paciente e a patogênese desta doença não é completamente conhecida. Este estudo testou a hipótese de que as células do infiltrado inflamatório podem abrigar betaherpesviruses e estes vírus estão ligados á subpopulação específicas de linfócitos. Fragmentos de tecido periodontal foram obtidas de pacientes afetados por periodontite e de indivíduos saudáveis. Imuno-histoquímica foi realizada para a contagem de células CD19+, céulas CD3+ e células CD4+ e CD8+. Reação em cadeia da polimerase e imuno-histoquímica foram realizados para detectar citomegalovírus, herpesvirus humanos 6 e 7 nas amostras. Como esperado, os tecidos coletados de indivíduos saudáveis não apresentaram nível significativo de infiltrado inflamatório e, portanto, foram excluídos dos procedimentos de imunofenotipagem. Os resultados mostraram que células CD19+ foram discretamente predomiantes sobre as células CD3+ no tecido periodontal afetado, mas estatisticamente não significativo. A subpopulação CD4+ de linfócitos estava significativamente em maior número que a subpopulação CD8+ de linfócitos (P=0...

‣ Correlação do citomegalovírus e do Herpesvírus Humano 8 nas infecções bacterianas em pacientes submetidos a transplante de fígado; Correlation of cytomegalovirus and Human Herpesvirus 8 in bacterial infections in patients undergoing liver transplantation

Arlete Milan
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 27/09/2013 Português
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O transplante de fígado tornou-se a terapia mais eficaz para o tratamento dos pacientes com doença hepática terminal, sendo que o sucesso pode ser limitado por complicações infecciosas no primeiro ano pós-transplante. O citomegalovírus é o tipo de infecção viral mais comum e está relacionado com a morbidade e mortalidade. A infecção bacteriana é uma séria complicação em pacientes transplantados de fígado. O Herpesvírus humano 8 tem uma distribuição global heterogênea, poucos estudos existem em pacientes submetidos à transplante de fígado. Nosso objetivo foi correlacionar a infecção do citomegalovírus e do Herpesvírus Humano 8 com a ocorrência de infecções bacterianas nos pacientes submetidos à transplante de fígado. Foram incluídos pacientes monitorados por seis meses para a detecção da infecção do citomegalovírus e do Herpesvírus Humano 8. As amostras foram realizadas no momento da cirurgia e semanalmente até o segundo mês, quinzenalmente no terceiro mês e mensalmente do quarto ao sexto mês. A infecção por citomegalovírus foi definida por antigenemia positiva (> três células) ou dois testes positivos de Nested-Reação em Cadeia de Polimerase com intervalo de 30 dias associada a sintomas clínicos. A metodologia para o diagnóstico de infecção bacteriana foi por meio de cultura de urina e de sangue através de testes bioquímicos e automatizado BacT / ALERT® 3 D - VITEK® (bioMérieux...

‣ Clinical features and outcomes of AIDS-related cytomegalovirus retinitis in the era of highly active antiretroviral therapy

Arantes,Tiago Eugênio Faria e; Garcia,Claudio Renato; Saraceno,Janaína Jamile Ferreira; Muccioli,Cristina
Fonte: Conselho Brasileiro de Oftalmologia Publicador: Conselho Brasileiro de Oftalmologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2010 Português
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PURPOSE: To describe the features and outcomes of patients with AIDS-related cytomegalovirus retinitis after highly active antiretroviral therapy availability. METHODS: Retrospective chart review of 30 consecutive patients (44 eyes) with AIDS and newly diagnosed, active AIDS-related cytomegalovirus retinitis, examined from January 2005 to December 2007. RESULTS: The mean age was 34.8 years, 18 patients (60.0%) were male and median duration of AIDS was 90 months. Nineteen patients (63.3%) had evidence of highly active antiretroviral therapy failure and median CD4+ lymphocyte count was 12.5 cells/µl. Visual acuity at presentation was 20/40 or better in 27 eyes (61.4%). Retinitis involved Zone 1 in 13 eyes (39.5%). Despite specific anti-AIDSrelated cytomegalovirus therapy, 16 eyes (36.4%) presented relapse of retinitis and 10 eyes (22.7%) lost at least three lines of vision. When compared to highly active antiretroviral therapy responsive patients, eyes of highly active antiretroviral therapy failure patients were more likely to develop relapse of retinitis (p=0.03) and loss of at least three lines of vision (p=0.03). CONCLUSION: The patients in this series are essentially young men with longstanding AIDS, non-responsive to highly active antiretroviral therapy and with a similar immunological profile as noted before highly active antiretroviral therapy era. These findings have implications for the management of the disease and confirm the magnitude of rational periodic screening after diagnosis of AIDS.

‣ Infection by cytomegalovirus in patients with neonatal cholestasis

OLIVEIRA,Nara Léia Gelle de; KANAWATY,Fernanda Rafful; COSTA,Sandra Cecilia Botelho; HESSEL,Gabriel
Fonte: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE ; Colégio Brasileiro de Cirurgia Digestiva - CBCD ; Sociedade Brasileira de Motilidade Digestiva - SBMD ; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED Publicador: Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE ; Colégio Brasileiro de Cirurgia Digestiva - CBCD ; Sociedade Brasileira de Motilidade Digestiva - SBMD ; Federação Brasileira de Gastroenterologia - FBG; Sociedade Brasileira de Hepatologia - SBH; Sociedade Brasileira de Endoscopia Digestiva - SOBED
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2002 Português
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Background - Neonatal cholestasis syndrome with an intra or extrahepatic origin has been associated to viral infections. The participation of the cytomegalovirus in the etiopathogenesis of neonatal hepatitis has been already known for some time, but only recently there have been indications that this virus may be one of the possible etiological factors for extrahepatic biliary atresia. Aims - To assess the prevalence of infection by cytomegalovirus in patients with intrahepatic cholestasis and extrahepatic cholestasis. To compare the clinical characteristics of the intrahepatic cholestasis and extrahepatic cholestasis groups with the cytomegalovirus serological results. Patients and Methods - This study consisted of 76 patients with neonatal cholestasis who were admitted between January 1980 and January 1999 when they underwent a cytomegalovirus serologic study using the ELISA method. A case note was kept on each patient with the following data: age of patient at admission, serologic result for cytomegalovirus, history of maternal infection, prematurity, fetal distress, birth weight, ponderal gain, choluria and fecal acholia. The final anatomic diagnosis of cholestasis was based on the results of an abdominal ultrasonography, a liver biopsy and its evolution. The patients were then divided into two groups: group I - intrahepatic cholestasis and group II - extrahepatic cholestasis. Each of these groups were then divided into two subgroups: subgroup A - positive serology (IgM) for cytomegalovirus and subgroup B - negative serology (IgM) for cytomegalovirus. Results - The frequency of positive serology (IgM) for cytomegalovirus was 29.4% in children with intrahepatic cholestasis and 28.5% in children with extrahepatic cholestasis. In comparison with group IIB...

‣ Cytomegalovirus seroepidemiology in an urban community of São Paulo, Brazil

Almeida,LNB; Azevedo,RS; Amaku,M; Massad,E
Fonte: Faculdade de Saúde Pública da Universidade de São Paulo Publicador: Faculdade de Saúde Pública da Universidade de São Paulo
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2001 Português
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INTRODUCTION: After the era of rubella vaccine, cytomegalovirus (CMV) infection is one of the most frequently causes of mental retardation and congenital deafness. Seroepidemiological studies are necessary to understand the transmission dynamics of the disease. The purpose of the study was to quantify the transmission rate of CMV disease in a community in the state of São Paulo, Brazil. METHODS: Using ELISA test (IgG), a retrospective serological survey looking for CMV antibodies was performed in an non-immunized community. Frozen sera from 443 individuals, randomly selected by cluster sampling technique in the town of Caieiras, São Paulo, were collected from November 1990 to January 1991. Seroprevalence was stratified by age (0-40 years). Mathematical techniques were applied to determine the age-dependent decay function of maternal antibodies during the first year of life, the age-dependent seroprevalence function and the force of infection for CMV in this community. RESULTS: It was observed a descending phase of seropositivity in the first 9 months, but changes in antibody titration were observed between 8 months old and one year of age. The average age of the first infection was 5.02 months of age and 19.84 years, when the age-dependent seroprevalence and the force of infection were analyzed between 10 months of age and 10 years of age and from 10 to 40 years old...

‣ Quantification of CD8+CD38+ T lymphocytes by flow cytometry does not represent a good biomarker to monitor the reactivation of cytomegalovirus infection after allogeneic hematopoietic stem cell transplantation

Lino,Vânia Abadia Soares; Santos,Silvana Maria Eloi; Bittencourt,Henrique Neves da Silva; Silva,Maria Luiza; Spizziri,Tiago; Bretas,Raquel; Neves,Suzane Pretti Figueiredo
Fonte: Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular Publicador: Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2011 Português
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BACKGROUND: Infection/reactivation of cytomegalovirus is a major cause of morbidity and mortality in immunocompromised transplant patients. It has already been observed in kidney and liver transplantation patients that cytomegalovirus disease is accompanied by significant increases in circulating CD8+CD38+ T lymphocytes. There are no reports that study CD8+CD38+ T lymphocytes to monitor/diagnose cytomegalovirus disease in hematopoietic stem cell transplantation patients. OBJECTIVE: The aim of this study was to evaluate some cellular activation markers on circulating mononuclear cells (CD38 and HLA-DR) in patients submitted to hematopoietic stem cell transplantation and to establish any correlation with cytomegalovirus disease as diagnosed by antigenemia. METHODS: Blood samples of 15 transplant patients were analyzed by flow cytometry using anti-CD3, anti-CD4, anti-CD8, anti-CD38, CD16, CD56 and anti-HLA-DR monoclonal antibodies and the results were evaluated in respect to cytomegalovirus antigenemia measured by indirect immunofluorescence. Minitab for Windows was used for statistical analysis and a p-value < 0.05 was considered significant. RESULTS: Patients with positive antigenemia did not show any significant increase in the percentages of cells expressing the CD38 or HLADR activation markers when compared to patients with negative antigenemia. On the contrary...

‣ Late cytomegalovirus infection after hematopoietic stem cell transplantation: case reports

Pinheiro,Samara Grapiuna; Matos,Socrates Bezerra de; Botura,Monica Borges; Meyer,Roberto; Lima,Fernanda Washington de Mendonca
Fonte: Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular Publicador: Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2013 Português
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Cytomegalovirus is related to high rates of morbidity and mortality after hematopoietic stem cell transplantation. This report highlights the importance of adequate monitoring and management of this infection. We report on two cases of patients with late subclinical cytomegalovirus infection. These patients were monitored for antigenemia by indirect immunofluorescence assay. Active cytomegalovirus infection is most common in the first three months after transplantation however the cases reported herein show the importance of monitoring for active infection after Day +100 post-transplantation. Early detection of active infection enables quick preemptive therapy. In conclusion, we emphasize that patients with risk factors for developing severe or late cytomegalovirus disease should be monitored for more than 100 post-transplant days as late active infection is a reality.

‣ PP65 antigenemia in the diagnosis of cytomegalovirus infection in AIDS patients

Capela,RC; Grassi,A; Souza,LR
Fonte: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP Publicador: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2012 Português
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Cytomegalovirus causes significant morbidity and mortality in AIDS patients and those having undergone bone marrow or another transplant. PP65 antigenemia is based on detecting viral antigen in peripheral blood leukocytes through immunochemistry and by monitoring the infection in immunocompromised individuals. The present study aimed to set up this diagnostic technique in AIDS patients with active cytomegalovirus infection and verify its occurrence in the Botucatu region of São Paulo state, Brazil. Fifty patients, 35 men and 15 women aged from 24 to 69 years, were recruited from those attended at the Department of Tropical Diseases of Botucatu Medical School, UNESP, and divided into three groups according to CD4+ T lymphocyte counts and antiretroviral treatment. The control group comprised bone marrow transplant patients. Fourteen AIDS patients with low CD4+ cell counts tested positive for PP65 antigenemia, which could predict cytomegalovirus infection and indicate prophylactic treatment.

‣ Cytomegalovirus infection in renal transplantation: clinical aspects, management and the perspectives

Requião-Moura,Lúcio Roberto; Matos,Ana Cristina Carvalho de; Pacheco-Silva,Alvaro
Fonte: Instituto Israelita de Ensino e Pesquisa Albert Einstein Publicador: Instituto Israelita de Ensino e Pesquisa Albert Einstein
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2015 Português
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Cytomegalovirus infection is one of most frequent infectious complications after renal transplantation, and can be classified as primo-infection, when the transmission occurs through the graft, or reactivation, when the recipient is cytomegalovirus seropositive. After transplantation, cytomegalovirus can appear as an infection, when the patient presents with evidence of viral replication without symptoms or disease, which has two clinical spectra: typical viral syndrome or invasive disease, which is a less common form. Their effects can be classified as direct, while the disease is developed, or indirect, with an increase of acute rejection and chronic allograft dysfunction risks. Diagnosis must be made based on viremia by one of the standardized methods: antigenemia or PCR, which is more sensitive. The risk factors related to infection after transplantation are the serologic matching (positive donor and negative recipient) and anti-lymphocyte antibody drugs. One of the strategies to reduce risk of disease should be chosen for patients at high risk: preemptive treatment or universal prophylaxis. Recent clinical research has described ganciclovir resistance as an emergent problem in management of cytomegalovirus infection. Two types of mutation that cause resistance were described: UL97 (most frequent) and UL54. Today...

‣ Impact of cytomegalovirus and grafts versus host disease on the dynamics of CD57+CD28-CD8+ T cells after bone marrow transplant

Mendes,Ana Verena Almeida; Kallas,Esper Georges; Benard,Gil; Pannuti,Cláudio Sérgio; Menezes,Reneé; Dulley,Frederico Luiz; Evans,Thomas George; Salomão,Reinaldo; Machado,Clarisse Martins
Fonte: Faculdade de Medicina / USP Publicador: Faculdade de Medicina / USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2008 Português
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OBJECTIVES: The present study aimed to evaluate the dynamics of CD28 and CD57 expression in CD8+ T lymphocytes during cytomegalovirus viremia in bone marrow transplant recipients. METHODS: In a prospective study, blood samples were obtained once weekly once from 33 healthy volunteers and weekly from 33 patients. To evaluate the expression of CD57 and CD28 on CD8+ T lymphocytes, flow cytometry analysis was performed on blood samples for four months after bone marrow transplant, together with cytomegalovirus antigenemia assays. RESULTS: Compared to cytomegalovirus-seronegative healthy subjects, seropositive healthy subjects demonstrated a higher percentage of CD57+ and a lower percentage of CD28+ cells (p<0.05). A linear regression model demonstrated a continuous decrease in CD28+ expression and a continuous increase in CD57+ expression after bone marrow transplant. The occurrence of cytomegalovirus antigenemia was associated with a steep drop in the percentage of CD28+ cells (5.94%, p<0.01) and an increase in CD57+ lymphocytes (5.60%, p<0.01). This cytomegalovirus-dependent effect was for the most part concentrated in the allogeneic bone marrow transplant patients. The development of acute graft versus host disease, which occurred at an earlier time than antigenemia (day 26 vs. day 56 post- bone marrow transplant)...

‣ Aktivität des neuen antiviralen Multiplex-Wirkstoffs AZT-Lipid-PFA im Mausmodell der intranasalen Cytomegalovirusinfektion; Activity of the new antiviral multiplex-drug AZT-Lipid-PFA in a mouse model of intranasal Cytomegalovirus infection

Schott, Sarah Julia
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
36.797976%
Eine Gruppe 70 gesunder Balb/c Mäuse wurde intranasal (i.n.) mit subletalen Dosen (2x104 PFU) des murinen Cytomegalovirus (MCMV) infiziert. Zur Ermittlung der antiviralen in vivo Wirkung des neuen Multiplex-Wirkstoffes AZT-Lipid-PFA, von dem bisher nur in vitro Daten bekannt waren, wurden von dieser Gruppe 35 Mäuse zusätzlich an den Tagen 1, 3, 5, 7, 9, 11, 13, 15, 17, 19 p.i. mit jeweils ca. 57 mg/kg dieses Wirkstoffs i.p. therapiert. Zur Kontrolle wurde mit einer zweiten Gruppe von 70 nicht infizierten Mäusen analog verfahren. Das Allgemeinbefinden der Mäuse änderte sich im Verlauf von 50 Tagen weder durch die Infektion noch durch die Therapie, wie aus Gewichtsmessungen gefolgert wurde. An den Tagen 1, 5, 15, 22, 29, 36, 43, 50 p.i. wurde die mRNA jeweils aus zehn verschiedenen Lungenabschnitten einer Lunge von jeweils drei getöteten Mäusen aus allen Gruppen isoliert. Die mRNA der verschiedenen Lungenabschnitte wurde mit Hilfe eines Oligo-dT-Primers und der RT-PCR-Methode in cDNA transkribiert. Durch eine Amplifikation der cDNA, wobei verschiedene spezifische Primer für die MCMV-spezifischen immediate early Gene ie 1, ie 3 und das early Gen gB eingesetzt wurden und der anschließenden elektrophoretischen Trennung der Amplifikationsansätze wurden Gelchromatogramme erhalten...

‣ Avaliação da infecção pelo citomegalovírus em pacientes com doença inflamatória intestinal; Cytomegalovirus infection evaluation in inflammatory bowel disease patients

Carmo, Alexandre Medeiros do
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 12/02/2014 Português
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36.857908%
Introdução: Citomegalovírus (CMV) é um DNA vírus de alta prevalência, e tem uma capacidade peculiar de infectar e permanecer integrado ao DNA das células do hospedeiro, mantendo-se na forma de infecção latente. O vírus também pode ocasionar doença, o que normalmente ocorre em pacientes imunocomprometidos, promovendo o aumento da morbidade e mortalidade nestes pacientes. As doenças inflamatórias intestinais (DII), doença de Crohn (DC) e retocolite ulcerativa (RCU), são enfermidades crônicas que afetam o trato gastrointestinal. A fisiopatologia e o tratamento destas doenças, muitas vezes, pode induzir um estado de imunossupressão. Isso incitou a ideia de que os pacientes com DII são mais susceptíveis à infecção e doença por CMV. Ainda há dúvidas e controvérsias sobre a relação entre a doença inflamatória intestinal e o CMV. Objetivos: Avaliar a frequência de infecção por CMV em pacientes com doença inflamatória intestinal, e se existe associação entre replicação viral do CMV com a atividade da DII, mediante índices clínicos e laboratoriais. Metodologia: Pacientes com DII previamente diagnosticada foram submetidos à entrevista, revisão de registros e coleta de amostras de sangue e fezes. Foram realizados os seguintes exames: pesquisa de citomegalovírus por IgG e IgM no sangue...

‣ Prevalence of toxoplasmosis, cytomegalovirus and rubella IgG antibodies in hatay women and children

Koksaldi-Motor,V; Evirgen,O; Azaroglu,I; Inci,M; Ozer,B; Arica,S
Fonte: West Indian Medical Journal Publicador: West Indian Medical Journal
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2012 Português
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36.857908%
OBJECTIVE: Toxoplasmosis, cytomegalovirus and rubella may cause congenital infections. The aim of this study is to investigate toxoplasmosis, cytomegalovirus and rubella IgG antibodies in women and children who were admitted to the Hatay Women and Children Hospital between January 1 and December 31, 2009. METHODS: Levels of IgG antibodies for toxoplasmosis, cytomegalovirus and rubella established with chemiluminescence immunoassay (Architect i2000, Abbott, USA) method were evaluated, retrospectively. RESULTS: The results were obtained from 1103 cases. Seropositivity of IgG antibodies for toxoplasmosis (n = 743), cytomegalovirus (n = 929) and rubella (n = 746) were 59.9%, 98.9% and 93.6%, respectively. CONCLUSION: Routine serological screening should be recommended in women of childbearing age. Seronegative subjects should be vaccinated for rubella and educated for protection from toxoplasmosis.