A melhor ferramenta para a sua pesquisa, trabalho e TCC!
Página 1 dos resultados de 4248 itens digitais encontrados em 0.052 segundos
- Biblioteca Digitais de Teses e Dissertações da USP
- Brazilian Archives of Biology and Technology
- Universidade Estadual Paulista (UNESP)
- Elsevier
- SETAC Press
- Instituto de Tecnologia do Paraná - Tecpar
- American Society for Microbiology
- American Society for Microbiology (ASM)
- Canadian Veterinary Medical Association
- Hindawi Publishing Corporation
- MIT - Massachusetts Institute of Technology
- Universidade de Tubinga
- Murcia : F. Hernández
- [Barcelona] : Universitat Autònoma de Barcelona,
- Universidade Autônoma de Barcelona
- Universidade Duke
- Mais Publicadores...
‣ Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media
Fonte: Tecpar
Publicador: Tecpar
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
67.487705%
DNA Microarray was developed to monitor the expression of many genes from Xylella fastidiosa, allowing the side by-side comparison of two situations in a single experiment. The experiments were performed using X. fastidiosa cells grown in two culture media: BCYE and XDM2. The primers were synthesized, spotted onto glass slides and the array was hybridized against fluorescently labeled cDNAs. The emitted signals were quantified, normalized and the data were statistically analyzed to verify the differentially expressed genes. According to the data, 104 genes were differentially expressed in XDM2 and 30 genes in BCYE media. The present study showed that DNA microarray technique efficiently differentiate the expressed genes under different conditions.; DNA Microarray foi desenvolvida para monitorar a expressão de muitos genes de Xylella fastidiosa, permitindo a comparação de duas situações distintas em um único experimento. Os experimentos foram feitos utilizando células de X. fastidiosa cultivada em dois meios de cultura: BCYE e XDM2. Pares de oligonucleotídeos iniciadores foram sintetizados, depositados em lâminas de vidro e o arranjo foi hibridizado contra cDNAs marcados fluorescentemente. Os sinais emitidos foram quantificados...
Link permanente para citações:
‣ Planejamento, gerenciamento e análise de dados de microarranjos de DNA para identificação de biomarcadores de diagnóstico e prognóstico de cânceres humanos; Planning, management and analysis of DNA microarray data aiming at discovery of biomarkers for diagnosis and prognosis of human cancers.
Fonte: Biblioteca Digitais de Teses e Dissertações da USP
Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado
Formato: application/pdf
Publicado em 12/05/2009
Português
Relevância na Pesquisa
57.459126%
#Análise de dados em larga escala#Cancer#Câncer#Classificadores#Classifiers#DNA microarrays#eXtreme Programming#Large-scale data analysis#Marcadores Moleculares#Microarranjos de DNA#Molecular Markers
Nesta tese, apresentamos nossas estratégias para desenvolver um ambiente matemático e computacional para análises em larga-escala de dados de expressão gênica obtidos pela tecnologia de microarranjos de DNA. As análises realizadas visaram principalmente à identificação de marcadores moleculares de diagnóstico e prognóstico de cânceres humanos. Apresentamos o resultado de diversas análises implementadas através do ambiente desenvolvido, as quais conduziram a implementação de uma ferramenta computacional para a anotação automática de plataformas de microarranjos de DNA e de outra ferramenta destinada ao rastreamento da análise de dados realizada em ambiente R. Programação eXtrema (eXtreme Programming, XP) foi utilizada como técnica de planejamento e gerenciamento dos projetos de análise dados de expressão gênica. Todos os conjuntos de dados foram obtidos por nossos colaboradores, utilizando-se duas diferentes plataformas de microarranjos de DNA: a primeira enriquecida em regiões não-codificantes do genoma humano, em particular regiões intrônicas, e a segunda representando regiões exônicas de genes humanos. A primeira plataforma foi utilizada para avaliação do perfil de expressão gênica em tumores de próstata e rim humanos...
Link permanente para citações:
‣ Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media
Fonte: Brazilian Archives of Biology and Technology
Publicador: Brazilian Archives of Biology and Technology
Tipo: Artigo de Revista Científica
Formato: 555-566
Português
Relevância na Pesquisa
67.487705%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 00/06289-2; DNA Microarray foi desenvolvida para monitorar a expressão de muitos genes de Xylella fastidiosa, permitindo a comparação de duas situações distintas em um único experimento. Os experimentos foram feitos utilizando células de X. fastidiosa cultivada em dois meios de cultura: BCYE e XDM2. Pares de oligonucleotídeos iniciadores foram sintetizados, depositados em lâminas de vidro e o arranjo foi hibridizado contra cDNAs marcados fluorescentemente. Os sinais emitidos foram quantificados, normalizados e os dados foram estatisticamente analisados para verificar os genes diferencialmente expressos. de acordo com nossos dados, 104 genes foram diferencialmente expressos para o meio de cultura XDM2 e 30 genes para o BCYE. No presente estudo, nós demonstramos que a técnica de DNA microarrays eficientemente diferencia genes expressos sob diferentes condições de cultivo.; DNA Microarray was developed to monitor the expression of many genes from Xylella fastidiosa, allowing the side by-side comparison of two situations in a single experiment. The experiments were performed using X. fastidiosa cells grown in two culture media: BCYE and XDM2. The primers were synthesized...
Link permanente para citações:
‣ Perfil transcricional de Bradyrhizobium elkanii SEMIA 587 in vitro e em simbiose com soja (Glycine max L. Merrill) através de microarranjo de DNA
Fonte: Universidade Estadual Paulista (UNESP)
Publicador: Universidade Estadual Paulista (UNESP)
Tipo: Tese de Doutorado
Formato: xvii, 139 f. : il.
Português
Relevância na Pesquisa
57.355186%
#Soja#Simbiose#Bradyrhizobium elkanii#Bacterióide#Bacteroid#Genic expression#DNA microarray#Bacterial metabolism#Transcriptional profile
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Pós-graduação em Microbiologia Agropecuária - FCAV; O nitrogênio é o nutriente requerido em maior quantidade para a cultura da soja. Avanços nas pesquisas de melhoramento genético vegetal e microbiologia do solo permitiram expandir o uso de inoculantes comerciais contendo estirpes de Bradyrhizobium japonicum e Bradyrhizobium elkanii. Estas bactérias infectam as raízes da planta e induzem a formação de nódulos, que abrigam a forma bacterióide, diferenciada da bactéria, responsável pela fixação simbiótica do nitrogênio. Informações sobre processos bioquímicos envolvidos no metabolismo da relação simbiótica podem ser adquiridas através de análises globais de expressão gênica. Para esta finalidade, destaca-se a tecnologia de microarranjo de DNA para detecção de genes diferencialmente expressos em larga escala. O objetivo geral deste trabalho foi identificar genes diferencialmente expressos, por meio de microarranjos de DNA, em Bradyrhizobium elkanii SEMIA 587 cultivada em diferentes meios de cultura, RDM (Rhizobia Defined Medium), TY (Triptone-Yeast Medium) e YMB (Yeast-Mannitol Medium), e em bacterióides isolados de nódulos de soja em diferentes períodos de desenvolvimento...
Link permanente para citações:
‣ A visual analytics framework for cluster analysis of DNA microarray data
Fonte: Elsevier
Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em //2013
Português
Relevância na Pesquisa
57.487705%
#Data mining#DNA-microarrays#Cluster analysis#Visual analytics#Metric spaces#Boundary points#Surface reconstruction
Prova tipográfica; Cluster analysis of DNA microarray data is an important but difficult task in knowledge discovery processes.
Many clustering methods are applied to analysis of data for gene expression, but none of them
is able to deal with an absolute way with the challenges that this technology raises. Due to this, many
applications have been developed for visually representing clustering algorithm results on DNA microarray
data, usually providing dendrogram and heat map visualizations. Most of these applications focus
only on the above visualizations, and do not offer further visualization components to the validate the
clustering methods or to validate one another. This paper proposes using a visual analytics framework
in cluster analysis of gene expression data. Additionally, it presents a new method for finding cluster
boundaries based on properties of metric spaces. Our approach presents a set of visualization components
able to interact with each other; namely, parallel coordinates, cluster boundary genes, 3D cluster
surfaces and DNA microarray visualizations as heat maps. Experimental results have shown that our
framework can be very useful in the process of more fully understanding DNA microarray data. The
software has been implemented in Java...
Link permanente para citações:
‣ Development of a microarray for Enchytraeus Albidus (oligochaeta): preliminary tool with diverse applications
Fonte: SETAC Press
Publicador: SETAC Press
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
57.247173%
Standard bioassays allow hazard assessment at the population level, but much remains to be learned about the molecular level response of organisms to stressors. The main aim of this study was the development of a DNA microarray for Enchytraeus albidus, a common soil worm species. Further, this microarray was tested using worms exposed to Cu, phenmedipham, and different soil types. Hybridization onto the developed microarray revealed several genes with homology to known sequences. Genes of interest were confirmed through real-time polymerase chain reaction. It was possible to discriminate between natural and chemical stressors and chemical concentrations. Gene responses were detected under conditions known to have effects in the reproduction of individuals. It was confirmed that the integration of different endpoints improves the assessment process and enhances the understanding of the modes of action of stressors. The chemical stress-induced genes were related to factors such as immune response, stress response, metabolic processes, and/or signal transduction. The present study represents the first step of a gene-level study in the ecologically relevant and standard test species E. albidus. It demonstrates the usefulness of cDNA normalization in the production of cDNA libraries of ecotoxicological standard organisms that are not genome models like E. albidus. Environ. Toxicol. Chem. 2011;30:1395-1402. (C) 2011 SETAC
Link permanente para citações:
‣ Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media
Fonte: Instituto de Tecnologia do Paraná - Tecpar
Publicador: Instituto de Tecnologia do Paraná - Tecpar
Tipo: Artigo de Revista Científica
Formato: text/html
Publicado em 01/06/2009
Português
Relevância na Pesquisa
67.34328%
DNA Microarray was developed to monitor the expression of many genes from Xylella fastidiosa, allowing the side by-side comparison of two situations in a single experiment. The experiments were performed using X. fastidiosa cells grown in two culture media: BCYE and XDM2. The primers were synthesized, spotted onto glass slides and the array was hybridized against fluorescently labeled cDNAs. The emitted signals were quantified, normalized and the data were statistically analyzed to verify the differentially expressed genes. According to the data, 104 genes were differentially expressed in XDM2 and 30 genes in BCYE media. The present study showed that DNA microarray technique efficiently differentiate the expressed genes under different conditions.
Link permanente para citações:
‣ Development and Clinical Evaluation of a Highly Sensitive DNA Microarray for Detection and Genotyping of Human Papillomaviruses
Fonte: American Society for Microbiology
Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /07/2004
Português
Relevância na Pesquisa
47.687886%
Human papillomavirus (HPV) has been found in cervical cancer, tonsillar cancer, and certain types of head and neck cancers. We report on a DNA microarray-based method for the simultaneous detection and typing of HPVs. The genotype spectrum discriminated by this HPV DNA microarray includes 15 high-risk HPV genotypes and 12 low-risk HPV genotypes. The HPV DNA microarray showed high degrees of specificity and reproducibility. We evaluated the performance of the HPV DNA microarray by application to three HPV-positive cell lines (HeLa, Caski, and SiHa cells) and two HPV-negative cell lines (C33A and A549 cells). The HPV DNA microarray successfully identified the known types of HPV present in the cell lines. The detection limit of the HPV DNA microarray was at least 100-fold higher than that of PCR. To assess the clinical applicability of the HPV DNA microarray, we performed the HPV genotyping assay with 73 nonmalignant and malignant samples from 39 tonsillar cancer patients. Twenty-five of the 39 (64.1%) malignant samples were positive for HPV, whereas 3 of 34 (8.8%) nonmalignant samples were positive for HPV. This result shows a preferential association of HPV with tonsillar carcinomas. The correlations of the presence of HPV with the grade of differentiation and risk factors were not significant. Our data show that the HPV DNA microarray may be useful for the diagnosis and typing of HPV in large-scale epidemiological studies.
Link permanente para citações:
‣ DNA Microarray-Based Detection and Identification of Fungal Pathogens in Clinical Samples from Neutropenic Patients▿
Fonte: American Society for Microbiology (ASM)
Publicador: American Society for Microbiology (ASM)
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
47.577007%
The increasing incidence of invasive fungal infections (IFI) in immunocompromised patients emphasizes the need to improve diagnostic tools. We established a DNA microarray to detect and identify DNA from 14 fungal pathogens (Aspergillus fumigatus, Aspergillus flavus, Aspergillus terreus, Candida albicans, Candida dubliniensis, Candida glabrata, Candida lusitaniae, Candida tropicalis, Fusarium oxysporum, Fusarium solani, Mucor racemosus, Rhizopus microsporus, Scedosporium prolificans, and Trichosporon asahii) in blood, bronchoalveolar lavage, and tissue samples from high-risk patients. The assay combines multiplex PCR and consecutive DNA microarray hybridization. PCR primers and capture probes were derived from unique sequences of the 18S, 5.8S, and internal transcribed spacer 1 regions of the fungal rRNA genes. Hybridization with genomic DNA of fungal species resulted in species-specific hybridization patterns. By testing clinical samples from 46 neutropenic patients with proven, probable, or possible IFI or without IFI, we detected A. flavus, A. fumigatus, C. albicans, C. dubliniensis, C. glabrata, F. oxysporum, F. solani, R. microsporus, S. prolificans, and T. asahii. For 22 of 22 patients (5 without IFI and 17 with possible IFI)...
Link permanente para citações:
‣ DNA microarray-based identification and typing of Actinobacillus pleuropneumoniae
Fonte: Canadian Veterinary Medical Association
Publicador: Canadian Veterinary Medical Association
Tipo: Artigo de Revista Científica
Publicado em /07/2009
Português
Relevância na Pesquisa
47.60728%
A DNA microarray system was prepared and shown to facilitate identification and typing of Actinobacillus pleuropneumoniae. The DNA microarray, composed of 18 DNA polymerase chain reaction (PCR) amplicons printed on glass slides and arranged in 3 subarrays, was developed. These target DNA included 1 or multiple fragments of the outer membrane lipoprotein, apx toxin, capsular polysaccharide, and disulfide bound formation protein E (dsbE)-like genes of A. pleuropneumoniae. These arrayed target DNA retained their expceted hybridization properties. The hybridization signal intensities ranged from the least-intense to the most-intense, 4626 to 9789 arbitrary fluorescence units, respectively. Cy3-probes of A. pleuropneumoniae strains labeled with multiplex PCR were hybridized to the DNA microarray. A total of 51 different A. pleuropneumoniae strains representing serotype 1 to 12 reference strains and clinical isolates were detected and typed by the DNA microarray. Twelve reference serotypes produced 11 distinct target DNA hybridization patterns, and hybridization patterns of serotypes 1 (n = 7), 3 (n = 5), and 7 (n = 6) field isolates were identical to hybridization patterns of reference serotypes 1, 3, and 7, respectively. Non-serotyped isolates 4...
Link permanente para citações:
‣ Comprehensive Analysis of Prokaryotes in Environmental Water Using DNA Microarray Analysis and Whole Genome Amplification
Fonte: MDPI
Publicador: MDPI
Tipo: Artigo de Revista Científica
Publicado em 30/10/2013
Português
Relevância na Pesquisa
47.56202%
The microflora in environmental water consists of a high density and diversity of bacterial species that form the foundation of the water ecosystem. Because the majority of these species cannot be cultured in vitro, a different approach is needed to identify prokaryotes in environmental water. A novel DNA microarray was developed as a simplified detection protocol. Multiple DNA probes were designed against each of the 97,927 sequences in the DNA Data Bank of Japan and mounted on a glass chip in duplicate. Evaluation of the microarray was performed using the DNA extracted from one liter of environmental water samples collected from seven sites in Japan. The extracted DNA was uniformly amplified using whole genome amplification (WGA), labeled with Cy3-conjugated 16S rRNA specific primers and hybridized to the microarray. The microarray successfully identified soil bacteria and environment-specific bacteria clusters. The DNA microarray described herein can be a useful tool in evaluating the diversity of prokaryotes and assessing environmental changes such as global warming.
Link permanente para citações:
‣ Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis
Fonte: Hindawi Publishing Corporation
Publicador: Hindawi Publishing Corporation
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
47.600474%
The paper describes comparative evaluation of IAVchip DNA microarray, reverse transcription PCR (RT-PCR), and real-time RT-PCR versus virus isolation in chicken embryos and shows their diagnostic effectiveness in detection and subtyping of influenza A virus. The tests were evaluated with use of 185 specimens from humans, animals, and birds. IAVchip DNA microarray demonstrates higher diagnostic effectiveness (99.45%) in early influenza A diagnosis as compared to the real-time PCR (98.38%) and RT-PCR (96.22%), thus showing its clear superiority. Diagnostic sensitivity of IAVchip DNA microarray (100%) exceeds the same of RT-PCR (95.95%) and real-time RT-PCR (97.96%) in the range of estimated confidence intervals. IAVchip DNA microarray and real-time RT-PCR displayed equal diagnostic specificity (98.85%), while diagnostic specificity of RT-PCR was 96.40%. IAVchip DNA microarray has an advantage over the other tests for influenza A diagnosis and virus identification as a more rapid method that allows performing simultaneous detection and subtyping of about tens of specimens within one experiment during 8–10 hours. The developed IAVchip DNA microarray is a general test tool that enables identifying simultaneously 16 hemagglutinin (HA) and 9 neuraminidase (NA) subtypes of influenza A virus and also to screen the influenza A viruses from humans...
Link permanente para citações:
‣ Hierarchical Multi-Bottleneck Classification Method And Its Application to DNA Microarray Expression Data
Fonte: MIT - Massachusetts Institute of Technology
Publicador: MIT - Massachusetts Institute of Technology
Tipo: Artigo de Revista Científica
Formato: 118852 bytes; application/pdf
Português
Relevância na Pesquisa
67.413696%
#DNA microarray#gene expression data#hierarchical multi-bottleneck classification method#Semi-parametric mixture identification
The recent development of DNA microarray technology is creating a wealth of gene expression data. Typically these datasets have high dimensionality and a lot of varieties. Analysis of DNA microarray expression data is a fast growing research area that interfaces various disciplines such as biology, biochemistry, computer science and statistics. It is concluded that clustering and classification techniques can be successfully employed to group genes based on the similarity of their expression patterns. In this paper, a hierarchical multi-bottleneck classification method is proposed, and it is applied to classify a publicly available gene microarray expression data of budding yeast Saccharomyces cerevisiae.; Singapore-MIT Alliance (SMA)
Link permanente para citações:
‣ Weiterentwicklung und Evaluierung eines DNS-Microarrays zur Identifikation von humanpathogenen Pilzen; Further development and evaluation of a DNA microarray for the identification of human pathogenic fungi
Fonte: Universidade de Tubinga
Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
67.687886%
#DNS-Chip , Pathogene Pilze , Medizinische Mikrobiologie#610#Dna microarray , Pathogenic fungi , Clinical microbiology
Invasive Mykosen stellen eine wachsende Gefahr, insbesondere für immunsupprimierte Patienten, dar. Dabei hängt die Prognose wesentlich von einer umgehend eingeleiteten spezifischen Therapie auf der Basis einer schnellen und präzisen Diagnose ab. Die bisherigen diagnostischen Methoden sind jedoch zeitaufwendig und die Ergebnisse oft zu unspezifisch.
Der 2004 am ITB Stuttgart entwickelte Oligonukleotid-Microarray zur Detektion von humanpathogenen Pilzen (Leinberger, 2004) ist in der Lage, ausgewählte Candida- und Aspergillusspezies schnell und zuverlässig zu identifizieren. In der vorliegenden Arbeit wurde dieses System von 12 Spezies aus 2 Gruppen auf 40 Spezies aus 15 Gruppen erweitert. Ein besonderer Schwerpunkt lag dabei auf den klinisch immer bedeutsamer werdenden Zygomyceten, von denen folgende Vertreter aufgenommen wurden: Absidia corymbifera und Absidia glauca, Mucor hiemalis, Rhizomucor pusillus, und Rhizopus oryzae.
Für die DNS Extraktion aus den Pilzzellen wurde ein neues Protokoll verwendet, dass die Arbeitszeit von vier auf eine Stunde verkürzte. Das Extraktionsergebnis wurde dadurch nicht beeinträchtigt: Aus allen Spezies konnte DNS in ausreichender Menge isoliert werden.
Um den optimalen Versuchsaufbau zu finden...
Link permanente para citações:
‣ Identification of gene networks modulated by activin in LßT2 cells using DNA microarray analysis
Fonte: Murcia : F. Hernández
Publicador: Murcia : F. Hernández
Tipo: Artigo de Revista Científica
Formato: application/pdf
Português
Relevância na Pesquisa
57.176763%
Activins, members of the TGFß family of
proteins, are widely expressed in a variety of tissues.
First identified based on their ability to regulate
biosynthesis and secretion of follicle-stimulating
hormone (FSH), activins have also been shown to
modulate development, cell growth, apoptosis, and
inflammation. Despite their many known functions, the
precise mechanisms and downstream signaling pathways
by which activins mediate their diverse effects remain
unknown. We have used a DNA microarray assay to
identify genes that are regulated by activin, alone or in
combination with gonadotropin-releasing hormone
(GnRH), another major regulator of FSH, in a murine
gonadotrope-derived cell line (LßT2). We used mRNA
from these cells to screen Affymetrix Mu74av2 mouse
Gene Chip oligonucleotide microarrays, representing
approximately 12,400 mouse genes. Treatment of LßT2
cells with activin A, a gonadotropin-releasing hormone
agonist (GnRHA) or activin A plus GnRHA resulted in
alterations in levels of gene expression that ranged in
magnitude from 15 to 67-fold. Data analysis identified
268 transcripts that were up- or down-regulated by twofold
or more. Distinct sets of genes were affected by
treatment with activin, GnRHA and activin plus
GnRHA...
Link permanente para citações:
‣ DNA Microarray Image Compression
Fonte: [Barcelona] : Universitat Autònoma de Barcelona,
Publicador: [Barcelona] : Universitat Autònoma de Barcelona,
Tipo: Tesis i dissertacions electròniques; info:eu-repo/semantics/doctoralThesis; info:eu-repo/semantics/publishedVersion
Formato: application/pdf
Publicado em //2015
Português
Relevância na Pesquisa
57.76905%
En los experimentos con DNA microarrays se genran dos imágenes monocromo, las cuales es conveniente almacenar para poder realizar análisis más precisos en un futuro. Por tanto, la compresión de imágenes surge como una herramienta particularmente útil para minimizar los costes asociados al almacenamiento y la transmisión de dichas imágenes. Esta tesis tiene por objetivo mejorar el estado del arte en la compresión de imágenes de DNA microarrays. Como parte de esta tesis, se ha realizado una detallada investigación de las características de las imágenes de DNA microarray. Los resultados experimentales indican que los algoritmos de compresión no adaptados a este tipo de imágenes producen resultados más bien pobres debido a las características de estas imágenes. Analizando las entropías de primer orden y condicionales, se ha podido determinar un límite aproximado a la compresibilidad sin pérdida de estas imágenes. Aunque la compresión basada en contexto y en segmentación proporcionan mejoras modestas frente a algoritmos de compresión genéricos, parece necesario realizar avances rompedores en el campo de compresión de datos para superar los ratios 2:1 en la mayor parte de las imágenes. Antes del comienzo de esta tesis se habían propuesto varios algoritmos de compresión sin pérdida con rendimientos cercanos al límite óptimo anteriormente mencionado. Sin embargo...
Link permanente para citações:
‣ Compressió d'imatges de DNA microarray basada en autosimilaridad
Fonte: Universidade Autônoma de Barcelona
Publicador: Universidade Autônoma de Barcelona
Tipo: info:eu-repo/semantics/bachelorThesis; Text
Formato: application/pdf
Publicado em 01/07/2014
Português
Relevância na Pesquisa
67.81672%
#Compressió d'imatges microarray#Imatges microarray dna#Codificació basada en autosimilaridad#Compresión de imágenes microarray#Imágenes microarray dna#Codificación basada en autosimilaridad#Compression of microarray images#DNA microarray images#Coding based on self-similarity
Els experiments DNA microarray generen milers de dades en forma d'imatges al voltant de tot món. Aquesta informació ha de ser eficientment emmagatzemada ja que cal guardar fins que les tècniques d'investigació estiguin cent per cent desenvolupades i estandarditzades. A partir de l'estudi de les característiques de les imatges DNA microarray, aquest article descriu un algorisme de compressió d'imatge sense pèrdua basat en la autosimilaridad de les mateixes imatges. S'han generat taules de dades a partir d'experimentar amb la codificació definida en aquest article. Dels resultats obtinguts s'observa que en determinats casos s'aconsegueix obtenir una millora de fins a gairebé 2 bpp a la compressió d'una imatge codificada respecte a una sense codificar pel algoritme basat en la autosimilaridad. En altres casos aquesta codificació significa fins a 3 bpp d'empitjorament en els resultats. En conclusió, amb una tècnica encara per millorar s'ha demostrat que utilitzant la autosimilaridad de les imatges DNA microarray per fer una codificació és possible obtenir millors resultats de compressió.; Los experimentos DNA microarray generan miles de datos en forma de imágenes alrededor de todo mundo. Esta información tiene que ser eficientemente almacenada ya que es necesario guardarla hasta que las técnicas de investigación estén cien por ciento desarrolladas y estandarizadas. A partir del estudio de las características de las imágenes DNA microarray...
Link permanente para citações:
‣ Analysis-driven lossy compression of DNA microarray images
Fonte: Universidade Autônoma de Barcelona
Publicador: Universidade Autônoma de Barcelona
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/acceptedVersion
Formato: application/pdf
Publicado em //2015
Português
Relevância na Pesquisa
67.56202%
DNA microarrays are one of the fastest-growing new technologies in the field of genetic research, and DNA microarray images continue to grow in number and size. Since analysis techniques are under active and ongoing development, storage, transmission and sharing of DNA microarray images need be addressed, with compression playing a significant role. However, existing lossless coding algorithms yield only limited compression performance (compression ratios below 2:1), whereas lossy coding methods may introduce unacceptable distortions in the analysis process. This work introduces a novel Relative Quantizer (RQ), which employs non-uniform quantization intervals designed for improved compression while bounding the impact on the DNA microarray analysis. This quantizer constrains the maximum relative error introduced into quantized imagery, devoting higher precision to pixels critical to the analysis process. For suitable parameter choices, the resulting variations in the DNA microarray analysis are less than half of those inherent to the experimental variability. Experimental results reveal that appropriate analysis can still be performed for average compression ratios exceeding 4.5:1.
Link permanente para citações:
‣ Compressió de microarrays d'ADN
Fonte: Universidade Autônoma de Barcelona
Publicador: Universidade Autônoma de Barcelona
Tipo: info:eu-repo/semantics/bachelorThesis; Text
Formato: application/pdf
Publicado em 30/06/2015
Português
Relevância na Pesquisa
47.67276%
#Microarray ADN#Compressió jpeg 2000 microarray#Imatge microarray adn rgb#Microarray color#Compresión jpeg 2000 microarray#Imagen microarray adn rgb#DNA microarray#JPEG 2000 microarray compression#DNA microarray RGB imatge#Color microarray
La tecnologia relacionada amb la creació d'imatges de microarray d'ADN és una eina de gran importància en el descobriment de l'estructura i funcionament de la nostra informació genètica. Dins d'aquest camp, la detecció del comportament de determinats gens sota condicions especifiques adquireix una gran rellevància. La grandària de les imatges de microarray sol ser de mitjana elevada, a causa de la gran quantitat de gens que s'analitzen i al fet que s'intenta mantenir en les imatges el major contingut d'informació possible. Aquestes són generades en grups de dues imatges i en escala de grisos. La finalitat d'aquest projecte és facilitar l'anàlisi de les imatges de microarray als especialistes a través de la combinació del parell d'imatges per formar una imatge en color en RGB, i alhora reduir la grandària d'aquestes imatges per no desaprofitar l'espai físic. En el camp dels microarrays d'ADN perdre informació en les imatges equival a perdre dades d'anàlisi, per aquesta raó la reducció de la mida de les imatges és realitza a través de la compressió sense pèrdua amb l'estàndard JPEG 2000, el que permet reduir la grandària d'aquestes alhora que manté la informació original continguda en elles.; The technology related to the DNA microarray image generation is a very important tool in the discovery of the structure and operation of our genetic information. Within this field...
Link permanente para citações:
‣ In situ synthesis of DNA microarray on functionalized cyclic olefin copolymer substrate.;
Fonte: Universidade Duke
Publicador: Universidade Duke
Tipo: Artigo de Revista Científica
Formato: 491 - 497
Publicado em /02/2010
Português
Relevância na Pesquisa
57.369946%
#Cycloparaffins#DNA#Equipment Design#Equipment Failure Analysis#Membranes, Artificial#Oligonucleotide Array Sequence Analysis#Polymers
Thermoplastic materials such as cyclic-olefin copolymers (COC) provide a versatile and cost-effective alternative to the traditional glass or silicon substrate for rapid prototyping and industrial scale fabrication of microdevices. To extend the utility of COC as an effective microarray substrate, we developed a new method that enabled for the first time in situ synthesis of DNA oligonucleotide microarrays on the COC substrate. To achieve high-quality DNA synthesis, a SiO(2) thin film array was prepatterned on the inert and hydrophobic COC surface using RF sputtering technique. The subsequent in situ DNA synthesis was confined to the surface of the prepatterned hydrophilic SiO(2) thin film features by precision delivery of the phosphoramidite chemistry using an inkjet DNA synthesizer. The in situ SiO(2)-COC DNA microarray demonstrated superior quality and stability in hybridization assays and thermal cycling reactions. Furthermore, we demonstrate that pools of high-quality mixed-oligos could be cleaved off the SiO(2)-COC microarrays and used directly for construction of DNA origami nanostructures. It is believed that this method will not only enable synthesis of high-quality and low-cost COC DNA microarrays but also provide a basis for further development of integrated microfluidics microarrays for a broad range of bioanalytical and biofabrication applications.
Link permanente para citações: