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‣ Stem cells in endometrium and their role in the pathogenesis of endometriosis

FIGUEIRA, Paula Gabriela Marin; ABRAO, Mauricio Simoes; KRIKUN, Graciela; TAYLOR, Hugh
Fonte: BLACKWELL SCIENCE PUBL Publicador: BLACKWELL SCIENCE PUBL
Tipo: Artigo de Revista Científica
Português
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The human endometrium is a dynamic tissue that undergoes cycles of growth and regression with each menstrual cycle. Adult progenitor stem cells are likely responsible for this remarkable regenerative capacity; these same progenitor stem cells may also have an enhanced capacity to generate endometriosis if shed in a retrograde fashion. The progenitor stem cells reside in the uterus; however, less-committed mesenchymal stem cells may also travel from other tissues such as bone marrow to repopulate the progenitor population. Mesenchymal stem cells are also involved in the pathogenesis of endometriosis and may be the principle source of endometriosis outside of the peritoneal cavity when they differentiate into endometriosis in ectopic locations. Finally, besides progenitor stem cells, recent publications have identified multipotent stem cells in the endometrium. These multipotent stem cells are a readily available source of cells that are useful in tissue engineering and regenerative medicine. Endometrial stem cells have been used to generate chondrocytes, myocytes, neurons, and adiposites in vitro as well as to replace dopaminergic neurons in a murine model of Parkinson`s disease.; NIH[U54HD052668]

‣ Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium

ROSSI, Alexandre Guilherme Zabeu; GOMES, Regina Celia Teixeira; SIMOES, Manuel de Jesus; SIMOES, Ricardo dos Santos; OLIVEIRA, Patricia B.; SOARES JR., Jose Maria; BARACAT, Edmund C.
Fonte: ELSEVIER SCIENCE INC; NEW YORK Publicador: ELSEVIER SCIENCE INC; NEW YORK
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
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Objective: To evaluate the effects of metoclopramide-induced hyperprolactinemia, on the prolactin receptor of murine endometrium. Design: Experimental study using the RNA extraction to detect tissue prolactin recepter isoforms by reverse-transcriptase polymerase chain reaction (RT-PCR). Setting: University-based laboratory. Animal(s): Seventy-two female swiss albino mice (Mus musculus), approximately 100 days old, were divided into six 12-animal groups: (Cl) nonoophorectomized mice given vehicle; (GII) nonoophorectomized mice treated with metoclopramide; (Gill) oophorectomized mice treated with metoclopramide; (GIV)oophorectomized mice treated with metoclopramide and 17 beta-estradiol; (GV) oophorectomized mice treated with metoclopramide and micronized progesterone; (GVI) oophorectomized mice treated with metoclopramide and a solution of 17 beta-estradiol and micronized progesterone. Intervention(s): Drugs were administered for 50 days. Following euthanasia, the middle portions of the uterine horns were removed, sectioned, and immediately frozen for RT-PCR procedures. Blood was collected for the dosage of prolactin and serum estrogen and progesterone using radioimmune assay. Main Outcome Measure(s): Identification of uterine prolactin receptor isoforms: Result(s): The PRL receptor and its isoform L were identified only in GI (control group) and GII (metoclopramide)...

‣ Differentially expressed genes in eutopic and ectopic endometrium of women with endometriosis

MEOLA, Juliana; SILVA, Julio Cesar Rosa e; DENTILLO, Daniel Blassioli; SILVA JR., Wilson Araujo da; VEIGA-CASTELLI, Luciana Caricati; BERNARDES, Luciano Angelo de Souza; FERRIANI, Rui Alberto; PAZ, Claudia Cristina Paro de; GIULIATTI, Silvana; MARTELLI, L
Fonte: ELSEVIER SCIENCE INC Publicador: ELSEVIER SCIENCE INC
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
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Objective: To elucidate the potential mechanisms involved in the physiopathology of endometriosis. We analyzed the differential gene expression profiles of eutopic and ectopic tissues from women with endometriosis. Design: Prospective laboratory study. Setting: University hospital. Patient(s): Seventeen patients in whom endometriosis was diagnosed and 11 healthy fertile women. Intervention(s): Endometrial biopsy specimens from the endometrium of healthy women without endometriosis and from the eutopic and ectopic endometrium tissues of patients with endometriosis were obtained in the early proliferative phase of the menstrual cycle. Main Outcome Measure(s): Six paired samples of eutopic and ectopic tissue were analyzed by subtractive hybridization. To evaluate the expression of genes found by rapid subtraction hybridization methods, we measured CTGF, SPARC, MYC, MMP and IGFBP1 genes by real-time polymerase chain reaction in all samples. Result(s): This study identified 291 deregulated genes in the endometriotic lesions. Significant expression differences were obtained for SPARC, MYC, and IGFBP1 in the peritoneal lesions and for MMP3 in the ovarian endometriomas. Additionally, significant differences were obtained for SPARC and IGFBP1 between the peritoneal and ovarian lesions. No significant differences were found for the studied genes between the control and the eutopic endometrium. Conclusion(s): This study identified 291 genes with differential expression in endometriotic lesions. The deregulation of the SPARC...

‣ Glycodelin expression in the endometrium of healthy women and in the eutopic and ectopic endometrium of women with endometriosis

MEOLA, Juliana; DENTILLO, Daniel Blassioli; SILVA, Julio Cesar Rosa e; FERRIANI, Rui Alberto; VEIGA, Luciana Caricati; PAZ, Cladia Cristina Paro de; GIULIATTI, Silvana; MARTELLI, Lucia
Fonte: ELSEVIER SCIENCE INC Publicador: ELSEVIER SCIENCE INC
Tipo: Artigo de Revista Científica
Português
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Objective: To analyze the expression of the glycodelin gene to better understand the molecular environment of endometriotic lesions and to elucidate the potential mechanisms that underlie the complex physiopathology of endometriosis. Design: Prospective laboratory study. Setting: University hospital. Patient(s): Eleven healthy fertile women and 17 patients with endometriosis in the early proliferative phase of the menstrual cycle. Intervention(s): Endometrial biopsy specimens were obtained from the endometrium of healthy women without endometriosis (controls) and from eutopic and ectopic endometrium tissues (pelvic and ovarian endometriotic implants) of endometriosis patients. Main Outcome Measure(s): The glycodelin relative expression level by real-time polymerase chain reaction (PCR) analysis. Result(s): The glycodelin down-regulation found in the endometriotic lesions was 332.26 and 123.17-fold lower, respectively, when compared with the eutopic tissue and the control endometrium. Conclusion(s): Glycodelin may be one of the molecules that contributes to the loss of cellular homeostasis in endometriotic lesions. (Fertil Steril (R) 2009;91:1676-80. (C)2009 by American Society for Reproductive Medicine.)

‣ Collagen fibril organization in the pregnant endometrium of decorin-deficient mice

SANCHES, Juliane C. T.; JONES, Carolyn J. P.; APLIN, John D.; IOZZO, Renato V.; ZORN, Telma M. T.; OLIVEIRA, Sergio F.
Fonte: WILEY-BLACKWELL PUBLISHING, INC Publicador: WILEY-BLACKWELL PUBLISHING, INC
Tipo: Artigo de Revista Científica
Português
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In the pregnant mouse endometrium, collagen fibrillogenesis is characterized by the presence of very thick collagen fibrils which are topographically located exclusively within the decidualized stroma. This dynamic biological process is in part regulated by the small leucine-rich proteoglycans decorin and biglycan. In the present study we utilized wild-type (Dcn+/+) and decorin-deficient (Dcn-/-) time-pregnant mice to investigate the evolution of non-decidualized and decidualized collagen matrix in the uterine wall of these animals. Ultrastructural and morphometric analyses revealed that the organization of collagen fibrils in the pregnant endometrium of both non-decidualized and decidualized stroma showed a great variability of shape and size, regardless of the genotype. However, the decidualized endometrium from Dcn-/- mice contained fibrils with larger diameter and more irregular contours as compared to the wild-type littermates. In the Dcn-/- animals, the proportion of thin (10-50 nm) fibrils was also higher as compared to Dcn+/+ animals. On day 7 of pregnancy, biglycan was similarly localized in the decidualized endometrium in both genotypes. Lumican immunostaining was intense both in decidualized and non-decidualized stroma from Dcn-/- animals. The present results support previous findings suggesting that decorin participates in uterine collagen fibrillogenesis. In addition...

‣ Perfil do RNAm da proteína transportadora de prostaglandina (PGT) no endométrio equino in vivo e sobre influência embrionária in vitro; mRNA to PGT profile in the equine endometrium in vivo, and under embryonic influence in vitro

Nascimento, Juliana
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 28/01/2011 Português
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Nas éguas cíclicas, a luteólise ocorre entre os dias 14 e 16 após ovulação, pela ação da PGF2ά endometrial. Entretanto, durante a gestação, a luteólise deve ser bloqueada, ao mesmo passo que a ação da PGE2 deve ser estimulada. Ambos hormônios possuem baixa difusão pela membrana plasmática, sendo necessária a presença da proteína transportadora de prostaglandina (PGT) para o influxo e efluxo destes hormônios nas células. Os objetivos deste experimento foram identificar e relacionar o RNAm da PGT no endométrio de éguas cíclica e gestante aos 14 dias (experimento 1) e avaliar o perfil do RNAm para PGT no endométrio eqüino em final de diestro sob efeito de secreção embrionária (experimento 2). Para o experimento 1, um ciclo estral de 11 éguas foi acompanhado. Seis éguas não foram inseminadas e somente detectado o tempo de ovulação e cinco foram inseminadas. Biópsias endometriais foram realizadas quando detectado folículo pré-ovulatório (≥35mm de diâmetro) e edema endometrial (E0; n=6), sete (E7; n=6) e quatorze (E14; n=6) dias após ovulação nas fêmeas cíclicas e aos quatorze dias de gestação (EG; n=4) nas fêmeas gestantes. No experimento 2, 5 embriões eqüinos de 13,5 dias de idade foram coletados...

‣ Expressão das moléculas reguladoras do sistema complemento, DAF e CD59, no endométrio de mulheres com abortos espontâneos recorrentes; Expression of Complement Regulatory Proteins, DAF e CD59, in endometrium of women with recurrent spontaneous abortion

Andozia, Mayra Beraldo
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 03/07/2009 Português
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Durante o ciclo menstrual, o endométrio se torna receptivo à chegada do "futuro embrião". Estes eventos são regulados pela liberação de progesterona durante a fase secretória do ciclo, que aumenta a secreção do componente C3 do Sistema Complemento (SC). Neste período, também foi observado o aumento fisiológico da expressão de algumas proteínas reguladoras do SC (CRPs) como: C1Inh, C4bp, DAF, CD59 e clusterina, oque sugere uma forte regulação da atividade deste sistema, favorável à implantação e ao desenvolvimento embrionário. Modificações na expressão destas proteínas poderiam resultar em aborto espontâneo recorrente (AER).Neste trabalho estudou-se a expressão endometrial de DAF e CD59 noendométrio de pacientes com AER, durante a fase secretória do ciclo. Adicionalmente, verificou-se a ativação do SC através da presença do neoantígeno de C9 neste mesmo material. A casuística foi composta de nove mulheres férteis normais (Grupo Controle) e doze mulheres com AER (Grupo Aborto), todas analisadas durante a fase secretória do ciclo. A técnica utilizada para detecção destas proteínas foi a imunohistoquímica. O neoantígeno deC9 não foi detectado em nenhum dos grupos, tanto nas glândulas quanto no estroma endometrial. DAF e CD59 foram detectados tanto no Grupo Controle quanto no Grupo Aborto.Houve expressão nitidamente mais acentuada de ambas as CRPs...

‣ Cycle modulation of insulin-like growth factor-binding protein 1 in human endometrium

Corleta, Helena von Eye; Capp, Edison; Strowitzki, T.
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
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Endometrium is one of the fastest growing human tissues. Sex hormones, estrogen and progesterone, in interaction with several growth factors, control its growth and differentiation. Insulin-like growth factor 1 (IGF-1) interacts with cell surface receptors and also with specific soluble binding proteins. IGF-binding proteins (IGF-BP) have been shown to modulate IGF-1 action. Of six known isoforms, IGF-BP-1 has been characterized as a marker produced by endometrial stromal cells in the late secretory phase and in the decidua. In the current study, IGF-1-BP concentration and affinity in the proliferative and secretory phase of the menstrual cycle were measured. Endometrial samples were from patients of reproductive age with regular menstrual cycles and taking no steroid hormones. Cytosolic fractions were prepared and binding of 125I-labeled IGF-1 performed. Crosslinking reaction products were analyzed by SDS-polyacrylamide gel electrophoresis (7.5%) followed by autoradiography. 125I-IGF-1 affinity to cytosolic proteins was not statistically different between the proliferative and secretory endometrium. An approximately 35-kDa binding protein was identified when 125I-IGF-1 was cross-linked to cytosol proteins. Secretory endometrium had significantly more IGF-1- BP when compared to proliferative endometrium. The specificity of the cross-linking process was evaluated by the addition of 100 nM unlabeled IGF-1 or insulin. Unlabeled IGF-1 totally abolished the radioactivity from the band...

‣ Avaliação histologica e da expressão genica de BMP e Wnt no endometrio durante a implantação embrionaria em bovinos; Histological and BMP and Wnt gene expression evaluation in the bovine endometrium during embryo implantation

Marlucia Bastos Aires
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 26/02/2009 Português
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A implantação embrionária implica em alterações profundas do ambiente uterino, cujos eventos devem obedecer uma sincronia temporal e espacial com o estágio do desenvolvimento embrionário. Qualquer desequilíbrio nesta sincronia resulta em interrupção da gestação e o desconhecimento dos mecanismos que atuam nas interações materno-fetais inviabilizam as possíveis intervenções buscando controlar as causas que levam às perdas iniciais da gestação. Visando contribuir para a melhor compreensão da biologia da resposta uterina de bovinos, o presente trabalho utilizou úteros de bovinos (Bos spp) não gestantes (NG) e gestantes nos períodos correspondentes à implantação embrionária para a coleta de fragmentos das regiões carunculares (CAR) e intercarunculares (IC). Esses fragmentos foram processadas para embebição em parafina destinadas às análises histológicas e obtenção de RNA total para realização de RT-PCR semi-quantitativo dos morfógenos Bmp2, Wnt2, Wnt5a, Wnt7a, e de seus antagonistas Sostdc1, Noggin, Dkk1 e Sfrp2. Pela análise histológica do desenvolvimento da mucosa uterina da região CAR foram estabelecidos os critérios morfológicos das seqüências de alterações da histoarquitetura endometrial em resposta à implantação embrionária constituindo quatro grupos (P1-P4) definindo os períodos gestacionais correspondentes...

‣ Autoradiography reveals regional metabolic differences in the endometrium of pregnant and nonpregnant mice

Oliveira,S.F.; Abrahamsohn,P.; Zorn,T.M.T.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/1998 Português
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The rodent endometrium undergoes remarkable modifications during pregnancy, resulting from a redifferentiation of its fibroblasts. During this modification (decidualization), the fibroblasts transform into large, polyhedral cells that establish intercellular junctions. Decidualization proceeds from the subepithelial stroma towards the deep stroma situated next to the myometrium and creates regions composed of cells in different stages of differentiation. We studied by autoradiography whether cells of these different regions have different levels of macromolecular synthesis. Radioactive amino acids or radioactive sulfate were administered to mice during estrus or on different days of pregnancy. The animals were killed 30 min after injection of the precursors and the uteri were processed for light microscope autoradiography. Silver grains were counted over cells of different regions of the endometrium and are reported as the number of silver grains per area. Higher levels of incorporation of amino acids were found in pregnant animals as compared to animals in estrus. In pregnant animals, the region of decidual cells or the region of fibroblasts transforming into decidual cells showed the highest levels of synthesis. Radioactive sulfate incorporation...

‣ Cycle modulation of insulin-like growth factor-binding protein 1 in human endometrium

Corleta,H.; Capp,E.; Strowitzki,T.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/11/2000 Português
Relevância na Pesquisa
37.23179%
Endometrium is one of the fastest growing human tissues. Sex hormones, estrogen and progesterone, in interaction with several growth factors, control its growth and differentiation. Insulin-like growth factor 1 (IGF-1) interacts with cell surface receptors and also with specific soluble binding proteins. IGF-binding proteins (IGF-BP) have been shown to modulate IGF-1 action. Of six known isoforms, IGF-BP-1 has been characterized as a marker produced by endometrial stromal cells in the late secretory phase and in the decidua. In the current study, IGF-1-BP concentration and affinity in the proliferative and secretory phase of the menstrual cycle were measured. Endometrial samples were from patients of reproductive age with regular menstrual cycles and taking no steroid hormones. Cytosolic fractions were prepared and binding of 125I-labeled IGF-1 performed. Cross-linking reaction products were analyzed by SDS-polyacrylamide gel electrophoresis (7.5%) followed by autoradiography. 125I-IGF-1 affinity to cytosolic proteins was not statistically different between the proliferative and secretory endometrium. An approximately 35-kDa binding protein was identified when 125I-IGF-1 was cross-linked to cytosol proteins. Secretory endometrium had significantly more IGF-1-BP when compared to proliferative endometrium. The specificity of the cross-linking process was evaluated by the addition of 100 nM unlabeled IGF-1 or insulin. Unlabeled IGF-1 totally abolished the radioactivity from the band...

‣ Canonical and noncanonical Wnt pathways: a comparison between endometrial cancer type I and atrophic endometrium in Brazil

Menezes,Marina de Pádua Nogueira; Oshima,Celina Tizuko Fujiyama; Badiglian Filho,Levon; Gomes,Thiago Simão; Barrezueta,Luis Fernando Mesias; Stávale,João Norberto; Gonçalves,Wagner José
Fonte: Associação Paulista de Medicina - APM Publicador: Associação Paulista de Medicina - APM
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2011 Português
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CONTEXT AND OBJECTIVE: The Wnt pathway is involved in tumorigenesis of several tissues. For this reason, we proposed to evaluate Wnt gene expression in endometrial cancer type I. DESIGN AND SETTING: Cross-sectional study on materials gathered from the tissue bank of the Department of Pathology, Universidade Federal de São Paulo. METHODS: Endometrial specimens were obtained from surgeries performed between 1995 and 2005 at São Paulo Hospital, Universidade Federal de São Paulo. The material was divided into two groups according to tissue type: Group A, atrophic endometrium (n = 15); and Group B, endometrial adenocarcinoma (n = 45). We compared the immunohistochemical expression of Wnt1, Frizzled-1 (FZD1), Wnt5a, Frizzled-5 (FZD5) and beta-catenin between endometrial cancer type I and atrophic endometrium. RESULTS: Regarding Wnt1, FZD1 and Wnt5a expression, no significant association was observed between the groups. A significant association was observed between the groups in relation to FZD5 expression (P = 0.001). The proportion of FZD5-positive samples was significantly higher in group A (80.0%) than in group B (31.1%). Regarding the survival curve for FZD5 in group B, we did not find any significant association between atrophic endometrium and endometrial adenocarcinoma. We also did not find any significant association regarding beta-catenin expression (P = 1.000). CONCLUSION: FZD5 is downregulated in endometrial adenocarcinoma...

‣ Simple hyperplasia versus proliferative endometrium: stereological study

Avvad-Portari,Elyzabeth; Gomes,Nádia D.; Mandarim-de-Lacerda,Carlos A.
Fonte: Sociedade Brasileira de Patologia Clínica; Sociedade Brasileira de Patologia; Sociedade Brasileira de Citopatologia Publicador: Sociedade Brasileira de Patologia Clínica; Sociedade Brasileira de Patologia; Sociedade Brasileira de Citopatologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2003 Português
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The diagnosis of some forms of hyperplastic endometrium continues to present difficulties for the practicing pathologist. One of the major problems is the lack of a standard terminology for endometrial classification. Therefore, morphometrical techniques could help the accurate diagnosis. Thirteen samples of endometrium with simple hyperplasia and thirteen samples of proliferative endometrium were analyzed by light microscopy and stereology. We determined the volume density, the surface density and the length density (Vv, Sv, Lv) of the glands (epithelium and lumen) and the stroma. Volume densities of the epithelium and lumen as well as the surface density of the inner perimeter of the glands were greater in simple hyperplasia than in proliferative endometrium (p < 0.05). But the volume density of the stroma was greater in proliferative endometrium than in simple hyperplasia (p < 0.05). The length density of the glands and the surface density of the outer perimeter were not different comparing these two groups. Present results agree with previous studies that have pointed out the importance of quantitative parameters in the diagnosis of the endometrial pathologies offering new stereological parameters to this analysis.

‣ Discovery of candidate genes and pathways in the endometrium regulating ovine blastocyst growth and conceptus elongation

Satterfield, M.; Song, G.; Kochan, K.; Riggs, P.; Simmons, R.; Elsik, C.; Adelson, D.; Bazer, F.; Zhou, H.; Spencer, T.
Fonte: Amer Physiological Soc Publicador: Amer Physiological Soc
Tipo: Artigo de Revista Científica
Publicado em //2009 Português
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Establishment of pregnancy in ruminants requires blastocyst growth to form an elongated conceptus that produces interferon tau, the pregnancy recognition signal, and initiates implantation. Blastocyst growth and development requires secretions from the uterine endometrium. An early increase in circulating concentrations of progesterone (P4) stimulates blastocyst growth and elongation in ruminants. This study utilized sheep as a model to identify candidate genes and regulatory networks in the endometrium that govern preimplantation blastocyst growth and development. Ewes were treated daily with either P4 or corn oil vehicle from day 1.5 after mating to either day 9 or day 12 of pregnancy when endometrium was obtained by hysterectomy. Microarray analyses revealed many differentially expressed genes in the endometria affected by day of pregnancy and early P4 treatment. In situ hybridization analyses revealed that many differentially expressed genes were expressed in a cell-specific manner within the endometrium. The Database for Annotation, Visualization, and Integrated Discovery (DAVID) was used to identify functional groups of genes and biological processes in the endometrium that are associated with growth and development of preimplantation blastocysts. Notably...

‣ Determination of the transcript profile of human endometrium

Borthwick, J.; Charnock-Jones, D.; Tom, B.; Hull, M.; Teirney, R.; Phillips, S.; Smith, S.
Fonte: Oxford University Press Publicador: Oxford University Press
Tipo: Artigo de Revista Científica
Publicado em //2003 Português
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The response of the human endometrium to the ovarian hormones, estrogen and progesterone, has been the focus of decades of research. In order to understand this critical aspect of endometrial physiology, we undertook a genome-wide analysis of transcript abundance and changes in transcript level between normal endometrium in the proliferative and secretory phases of the menstrual cycle. A high-density, oligonucleotide gene array, comprising 60 000 gene targets, was used to define the gene expression profile of proliferative and secretory phase endometrium. Results from the arrays were verified using real-time PCR. The expression levels of 149 transcripts differed signi®cantly between the two phases of the cycle determined by stringent range limits (99.99%), calculated using local variance values. These transcripts include previously documented steroidally responsive genes (such as placental protein 14 and stromelysin-3) and novel transcripts not previously linked to either endometrial physiology or steroid regulation (such as intestinal trefoil factor and a number of expressed sequence tags). Examination of the 5¢ promoter regions of these genes identified many putative estrogen and progesterone receptor DNA binding domains, suggesting a direct response of these genes to the ovarian hormones.; Jane M.Borthwick...

‣ Evaluation von Normwerten des sonographischen Endometriums- und Flüssigkeitsreflexes des Cavum uteri post partum; Evaluation of normal data of sonographic endometrium and fluid reflex of cavum uteri after delivery

Geissl-de Both, Judith
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
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Zielsetzung der Studie war es, anhand einer grossen Fallzahl Frauen postpartal - unabhängig ihres Geburtsmodus - sonographisch zu untersuchen, um Normwerte des sagittalen bzw. transversalen Endometriums- und Flüssigkeitsreflexes zu definieren. 6 bis 12 Monate postpartal wurde von den Patientinnen ein zusätzlicher Fragebogen zu dieser Studie beantwortet. Ein weiteres Ziel dieser Arbeit bestand darin, das Lochialsekret zu quantifizieren und herauszufinden, ob und ab wann sonographisch ein Rückstau oder eine beginnende Endomyometritis erfassbar und somit rechtzeitig therapierbar sind. Von Juni 2003 bis Juli 2004 wurden 508 Frauen postpartal untersucht. Die erhobenen Werte des Endometriumsreflexes zeigten in den sagittalen und transversalen Schnittebenen eine enorme Variationsbreite (zwischen 1.7mm und 48.5mm). Beim Vergleich der Werte der unterschiedlichen Entbindungsmodi ergab sich, dass die sagittalen Mediane signifikant divergieren (Gesamtmedian: 10.2mm; vaginal: 12.5mm; VE: 11.8mm; Forceps: 22.9mm; primäre Sectio: 6.6mm; sekundäre Sectio: 6.7mm; Notsectio: 5.8mm). Bei 50% lies sich ein nach den Kriterien von Hertzberg (1991) und Carlan (1997) normal definierter Endometriumsreflex nachweisen. 2% dieser Frauen erhielten postpartal eine Abrasio. In keinem Fall war ein Lochialstau aufgetreten Die einzige Endomyometritis kam in dieser Gruppe vor. In 38% war ein inhomogener Reflex nachweisbar (postpartale Abrasio: 2.7%; Lochialstau: 4.8%). Ein echoreicher Reflex war in 10% nachweisbar (postpartale Abrasio: 2%; Lochialstau: 15%). In 3% war ein echoarmer Reflex nachweisbar (postpartale Abrasio: 7%; Lochialstau 0%) Schlussfolgerung: Der als normal definierte Endometriumsreflex ist somit der häufigste postpartale Sonographiebefund. Der Endometriums- und Flüssigkeitsreflex post partum weist messtechnisch nur sehr bedingt eindeutige Unterschiede bei postpartalen Komplikationen auf. Daher sind sonographischen Messwerte als alleinige prognostische Faktoren für das Auftreten von postpartalen Komplikationen wie Lochialstau...

‣ Local immune response in serous papillary carcinoma of the endometrium

Tamiolakis, D.; Venizelos, J.; Lambropoulou, M.; Nikolaidou, S.; Tsikouras, P.; Jivannakis, T.; Papadopoulos, N.
Fonte: Murcia : F. Hernández Publicador: Murcia : F. Hernández
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
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Objective: Serous papillary carcinomas of the endometrium are aggressive tumors that tend to permeate, in a very extensive fashion, to uterine and adnexal lymphatic and vascular channels at an early stage in their evolution, and are associated with a particularly gloomy prognosis. It is generally thought that even tumors apparently limited to the endometrium or confined to an endometrial polyp have a poor outcome. Our study points towards the value of HLADR antigen in the outcome of serous papillary endometrial cancer. Our aim was to assess the HLA-DR expression in inactive, endometrial intraepithelial carcinoma (EIC), and invasive serous carcinoma curretage specimens from the endometrial cavity, suggesting a role inimmune response to keep tumor proliferation in check. Study design: Thirty-one cases of inactive endometrium, twelve cases of EIC, and thirtynine cases of serous papillary invasive carcinoma curettings were evaluated for the detection of HLA-DR monoclonal antigen. T helper (TH) marker (CD4) in the tumor stroma of the relevant cases was also studied, given that it is now known that the dependence of immune responsiveness on the class II antigens reflects the central role of these molecules in presenting antigen to TH cells. Results: HLA-DR was expressed in 20 of 31 inactive endometrium (64.5%)...

‣ Steroid receptors ERa, ERß, PR-A and PR-B are differentially expressed in normal and atrophic human endometrium

Mylonas, I.; Jeschke, U.; Shabani, N.; Kuhn, C.; Kunze, Sussane; Dian, D.; Friedl, C.; Kupka, M.S.; Friese, K.
Fonte: Murcia : F. Hernández Publicador: Murcia : F. Hernández
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
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Objective: The endometrium expresses estrogen (ER) and progesterone receptor (PR), which are related to autocrine and paracrine processes that respond to estrogen and progesterone. Therefore, the aim of this study was to evaluate the distribution pattern of ERa, ERß, PR-A and PR-B with monoclonal antibodies in normal human endometrial tissue. Study Design: Human endometrial tissue was obtained from 84 premenopausal and 11 postmenopausal patients and immunohistochemically analysed with monoclonal antibodies against ERa, ERß, PR-A and PR-B. Results: ERa, PR-A and PR-B declined significantly (p<0.001, p<0.05, p<0.05 respectively) in glandular epithelium from proliferative to late secretory phase. The ERß immunohistochemical reaction showed a similar significant declining pattern (p<0.05), although the staining intensity was lower than that of ERa. While ERa, ERß and PR-B decrease significantly in atrophic endometrial tissue compared to proliferative endometrium, a significant up-regulation of PR-A was observed compared to late secretory phase (p<0.05). Conclusion: ERa, ERß, PR-A and PR-B were expressed in normal human endometrium with a cyclical variation during the menstrual cycle. In normal postmenopausal endometrial tissue, a down-regulation of ERa...

‣ Physiologic activation of nuclear factor kappa-B in the endometrium during the menstrual cycle is altered in endometriosis patients

Kohen, Paulina; Rojas, Candy; Alvarado Díaz, Carlos; Poch, Andrea; Devoto, Luigi; Rocco, Jocelyn; Sovino, Hugo; González Ramos, Reinaldo
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo de revista
Português
Relevância na Pesquisa
37.30681%
Objective: To evaluate nuclear factor kappaB (NF-kB) activation and NF-kB–p65 subunit activation, immunolocalization, and expression in the endometrium of healthy women and endometriosis patients throughout the menstrual cycle. Design: Prospective observational study. Setting: Affiliated hospital and university research laboratory. Patient(s): Twenty-four healthy women and 24 endometriosis patients. Intervention(s): Menstrual, proliferative, and secretory endometrial biopsies. Main Outcome Measure(s): Assessment of NF-kB and p65 activation by protein-DNA binding assays and p65 localization and expression by immunohistochemistry. Result(s): Total NF-kB–DNA binding was constitutive and variable in human endometrium accross the menstrual cycle. Healthy women (physiologic conditions) showed higher p65-DNA binding in proliferative than in menstrual and secretory endometrium. Conversely, in endometriosis patients, p65-DNA binding was higher in proliferative and secretory endometrium than in menstrual endometrium. Endometrial epithelial cells showed higher p65 expression level score than endometrial stromal cells. Conclusion(s): NF-kB activity is constitutive, physiologic, and variable in human endometrium. The physiologic cyclic p65 activation pattern was altered in endometriosis patients...

‣ Histomorphometry of epithelial structures of the mare’s endometrium

Mansour, Geórgia Duna; Pathology Department, Universidade Federal Fluminense, Niterói, RJ, Brazil. Endereço: Rua Cupertino Durão 25/107 Leblon. CEP: 22441-030.; Ferreira, Ana Maria Reis; Pathology Department, Universidade Federal Fluminense, Niterói,
Fonte: Universidade Federal Fluminense Publicador: Universidade Federal Fluminense
Tipo: info:eu-repo/semantics/article; RA; Artigo Original; info:eu-repo/semantics/publishedVersion Formato: application/pdf
Publicado em 27/11/2013 Português
Relevância na Pesquisa
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The aim of this study was to analyze through histomorphometry, epithelial structures of the mare’s endometrium classifiedaccording to Kenney and Doig (1986). Histomorphometric analysis of the luminal and glandular epithelial cell heights, glandularlumen diameter, glandular density, percentage of glands with apparent lumen and with intraluminal secretion, and a descriptiveanalysis of the endometrium was made in 65 endometrial biopsies. Samples were collected during estrus (n=30) and diestrus(n=35), fixed in Bouin’s solution, embedded in paraffin HxE and classified according to Kenney and Doig (1986) categories.Groups of IIa category obtained the greatest medium cell heights for luminal and glandular epithelium, and groups of III categoryobtained the smallest values, during estrus and diestrus. The medium heights of luminal epithelium, glandular epithelium andglandular lumen diameter of samples collected during estrus were greater than those collected during diestrus. The glanddensity was greater during diestrus than estrus. This work showed that histomorphometry could improve the consistency inbiopsy evaluation by furnishing objective data.