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‣ Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy

MARCACCINI, Andrea M.; MESCHIARI, Cesar A.; ZUARDI, Leonardo R.; SOUSA, Tiago Sampaio de; TABA JR., Mario; TEOFILO, Juliana M.; JACOB-FERREIRA, Anna L. B.; TANUS-SANTOS, Jose E.; NOVAES JR., Arthur B.; GERLACH, Raquel F.
Fonte: WILEY-BLACKWELL PUBLISHING, INC Publicador: WILEY-BLACKWELL PUBLISHING, INC
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
100.02445%
P>Background This study aimed at comparing the levels of matrix metalloproteinase (MMP)-8, tissue Inhibitor of MMPs (TIMP)-1 and TIMP-2, Myeloperoxidase (MPO), and MMP-9 in the gingival crevicular fluid (GCF) of chronic periodontitis (CP) patients and controls at baseline and 3 months after non-surgical therapy. Materials and Methods GCF was collected from one site of 15 control subjects and 27 CP patients. MMP-8, MMP-9, TIMP-1, and TIMP-2 were determined by Enzyme-linked immunoabsorbent assay; different forms of MMP-9, by gelatin zymography; and MPO, colorimetrically. Results At baseline, higher levels of MMP-8, TIMP-2, MPO, and the 87 kDa-MMP-9 were found in patients compared with controls (p < 0.001), and these molecules decreased after therapy (p < 0.03). There were no differences between the groups with respect to the higher molecular forms of MMP-9 (180, 130, 92 kDa) or total MMP-9 at baseline. No differences were observed in TIMP-1 levels. In controls, decreased levels of TIMP-2 and the higher molecular forms of MMP-9 (180, 130, 92 kDa) were found 3 months after therapy compared with baseline (p < 0.01). Conclusions Higher levels of MMP-8, TIMP-2, MPO, and 87 kDa MMP-9 were found in the GCF of patients compared with controls...

‣ Haplotypes of susceptibility to chronic periodontitis in the Interleukin 8 gene do not influence protein level in the gingival crevicular fluid

Corbi, Samia C. T.; Anovazzi, Giovana; Finoti, Livia S.; Kim, Yeon J.; Capela, Marisa V.; Secolin, Rodrigo; Marcaccini, Andrea M.; Gerlach, Raquel F.; Orrico, Silvana R. P.; Cirelli, Joni A.; Scarel-Caminaga, Raquel M.
Fonte: PERGAMON-ELSEVIER SCIENCE LTD; OXFORD Publicador: PERGAMON-ELSEVIER SCIENCE LTD; OXFORD
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
100.02445%
Objective: Previously, we identified that the ATC/TTC haplotype formed by polymorphisms in the Interleukin-(IL)8 gene conferred susceptibility to chronic periodontitis (CP). The aim of the study was to investigate whether the IL8 haplotype ATC/TTC was associated with the volume of gingival crevicular fluid (GCF), the concentration of interleukin IL-8 in the GCF, as well as periodontal conditions in patients with CP in comparison to controls without CP. Methods: Seventy-nine individuals (CP: n = 41, controls: n = 38) were grouped according to the presence (susceptible for CP) or absence (not susceptible for CP) of the IL8 ATC/TTC haplotype. After periodontal clinical evaluation, they were subdivided by the presence or absence of CP. GCF was collected from each patient and the IL-8 levels were determined by ELISA. The GCF volume of each subject was measured by means of a calibrated electronic device. Comparisons of means between carriers and non-carriers of the ATC/TTC haplotype were evaluated using the Mann-Whitney test. Linear regression and stepwise linear regression analysis were used to analyse the association of the GCF volume with potential covariates and their contribution for the phenotype. Results: We did not find significant differences of both periodontal conditions and IL-8 concentration in the GCF of patients with the presence or absence of the IL8 ATC/TTC haplotype. However...

‣ Efeito do tratamento periodontal não cirúrgico na atividade da peroxidase na saliva da glândula parótida e no fluído gengival; Effect of non Surgical Periodontal Therapy on Peroxidase Activity in Parotid Saliva and Gingival Crevicular Fluid

Gonçalves, Rogéria Pereira
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 10/09/2012 Português
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Dois importantes fatores inter-relacionados estão envolvidos na patogênese da doença periodontal: ativação do sistema imune e produção de peroxidases. Os objetivos deste estudo foram comparar os níveis da atividade do sistema peroxidase presentes no fluido gengival (MPO/FG) e saliva da parótida (POS) em pacientes portadores de periodontite crônica em relação a pacientes periodontalmente saudáveis e avaliar o efeito do tratamento periodontal não cirúrgico nestes indivíduos nestes parâmetros enzimáticos. MATERIAL E MÉTODO: Foram coletados dados dos parâmetros clínicos periodontais e amostras de FG e saliva da glândula parótida de 17 pacientes clinicamente saudáveis (GC) e de 17 pacientes portadores de periodontite crônica generalizada (GP) antes e depois do tratamento periodontal pareados em idade e sexo. As atividades de MPO/FG e POS Parótida foram avaliadas por espectofotometria. RESULTADOS: O GP apresentou valores maiores nos parâmetros clínicos periodontais em relação ao GC, sendo que os mesmos foram reduzidos após tratamento periodontal. Além disso, o GP apresentou valores mais elevados de atividade de MPO no FG (p=0,04) do que o GC. Após tratamento periodontal os níveis de MPO/FG (p<0,001) e POS parótida (p=0...

‣ Níveis salivares e de fluido gengival de Mieloperoxidase/Peroxidase em pacientes portadores de diabetes mellitus tipo 2 com periodontite crônica generalizada; Salivary and gingival crevicular fluid levels of Myeloperoxidase/Peroxidase in type 2 diabetes mellitus patients with generalized chronic periodontitis

Benedete, Ana Paula Sassá
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 26/06/2013 Português
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Objetivos: Avaliar os níveis de mieloperoxidase do fluido gengival (MPO) e de peroxidase salivar (SPO) de pacientes portadores de diabetes mellitus tipo 2 com periodontite crônica generalizada (DMDP) antes e após o tratamento periodontal. Material e Métodos: Participaram do estudo 31 indivíduos com DMDP, 31 controle com DM 2 (DM), 31 com periodontite crônica generalizada sem diabetes (DP) e 31 Controles(C) saudáveis periodontalmente e sistemicamente. Foram coletados dados de parâmetros clínicos como profundidade clínica de sondagem (PCS), nível clínico de inserção (NCI), sangramento à sondagem (SS), índice de placa (IP) e amostras de sangue para hemoglobina glicada, saliva e fluido gengival (FG) de todos os pacientes antes e 30 dias após o tratamento periodontal (TP). A atividade das enzimas foi determinada pela técnica de espectrofotometria. Os dados clínicos e enzimáticos foram analisados por métodos estatísticos não-paramétricos. Resultados: No baseline, a atividade da MPO estava maior no grupo DMDP: 1893,27 comparado ao grupo DM: 1202,73 (p0,015) e ao grupo C: 6,46 (p0,001). Também se mostrou mais elevada no grupo DP: 2196,10 em relação ao grupo C: 6,46 (p0,007). O tratamento periodontal se mostrou efetivo na redução de MPO nos grupos DMDP e DP (p0...

‣ The Short-Term Effectiveness of Non-Surgical Treatment in Reducing Levels of Interleukin-1 beta and Proteases in Gingival Crevicular Fluid From Patients With Type 2 Diabetes Mellitus and Chronic Periodontitis

Correa, Fernanda O. B.; Goncalves, Daniela; Figueredo, Carlos M. S.; Gustafsson, Anders; Orrico, Silvana R. P.
Fonte: Amer Acad Periodontology Publicador: Amer Acad Periodontology
Tipo: Artigo de Revista Científica Formato: 2143-2150
Português
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Processo FAPESP: 04/08142-0; Background: This study aimed to compare the effectiveness of nonsurgical periodontal treatment in improving periodontal status and reducing gingival crevicular fluid (GCF) levels of interleukin (IL)-1 beta and IL-18, elastase activity, and matrix metalloproteinase (MMP)-8 and -9 in periodontitis patients with and without type 2 diabetes mellitus (T2DM).Methods: Twenty-three patients with T2DM (diabetes group) and 26 systemically healthy subjects (control group) with chronic periodontitis participated in this study. The clinical examination included visible plaque index, gingival bleeding index, probing depth, clinical attachment level, and bleeding on probing. GCF samples were collected from five or six deep sites to evaluate the levels of IL-1 beta and -18, elastase, and MMP-8 and -9. Shallow sites were analyzed for IL-1 beta and elastase. The glycemic control was analyzed by the concentration of glycated hemoglobin (HbA1c). The subjects received non-surgical periodontal treatment and were reexamined 90 days later.Results: All clinical parameters showed a significant improvement after treatment...

‣ Haplotypes of susceptibility to chronic periodontitis in the Interleukin 8 gene do not influence protein level in the gingival crevicular fluid

Corbi, Samia C. T.; Anovazzi, Giovana; Finoti, Livia S.; Kim, Yeon J.; Capela, Marisa V.; Secolin, Rodrigo; Marcaccini, Andrea M.; Gerlach, Raquel F.; Orrico, Silvana R. P.; Cirelli, Joni A.; Scarel-Caminaga, Raquel M.
Fonte: Pergamon-Elsevier B.V. Ltd Publicador: Pergamon-Elsevier B.V. Ltd
Tipo: Artigo de Revista Científica Formato: 1355-1361
Português
Relevância na Pesquisa
100.02445%
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Processo FAPESP: 03/10424-0; Processo FAPESP: 09/08773-3; Objective: Previously, we identified that the ATC/TTC haplotype formed by polymorphisms in the Interleukin-(IL)8 gene conferred susceptibility to chronic periodontitis (CP). The aim of the study was to investigate whether the IL8 haplotype ATC/TTC was associated with the volume of gingival crevicular fluid (GCF), the concentration of interleukin IL-8 in the GCF, as well as periodontal conditions in patients with CP in comparison to controls without CP.Methods: Seventy-nine individuals (CP: n = 41, controls: n = 38) were grouped according to the presence (susceptible for CP) or absence (not susceptible for CP) of the IL8 ATC/TTC haplotype. After periodontal clinical evaluation, they were subdivided by the presence or absence of CP. GCF was collected from each patient and the IL-8 levels were determined by ELISA. The GCF volume of each subject was measured by means of a calibrated electronic device. Comparisons of means between carriers and non-carriers of the ATC/TTC haplotype were evaluated using the Mann-Whitney test. Linear regression and stepwise linear regression analysis were used to analyse the association of the GCF volume with potential covariates and their contribution for the phenotype.Results: We did not find significant differences of both periodontal conditions and IL-8 concentration in the GCF of patients with the presence or absence of the IL8 ATC/TTC haplotype. However...

‣ Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy

MARCACCINI, Andrea M.; MESCHIARI, Cesar A.; ZUARDI, Leonardo R.; SOUSA, Tiago Sampaio de; TABA JR., Mario; TEOFILO, Juliana M.; JACOB-FERREIRA, Anna L. B.; TANUS-SANTOS, Jose E.; NOVAES JR., Arthur B.; GERLACH, Raquel F.
Fonte: WILEY-BLACKWELL PUBLISHING, INC Publicador: WILEY-BLACKWELL PUBLISHING, INC
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
100.02445%
P>Background This study aimed at comparing the levels of matrix metalloproteinase (MMP)-8, tissue Inhibitor of MMPs (TIMP)-1 and TIMP-2, Myeloperoxidase (MPO), and MMP-9 in the gingival crevicular fluid (GCF) of chronic periodontitis (CP) patients and controls at baseline and 3 months after non-surgical therapy. Materials and Methods GCF was collected from one site of 15 control subjects and 27 CP patients. MMP-8, MMP-9, TIMP-1, and TIMP-2 were determined by Enzyme-linked immunoabsorbent assay; different forms of MMP-9, by gelatin zymography; and MPO, colorimetrically. Results At baseline, higher levels of MMP-8, TIMP-2, MPO, and the 87 kDa-MMP-9 were found in patients compared with controls (p < 0.001), and these molecules decreased after therapy (p < 0.03). There were no differences between the groups with respect to the higher molecular forms of MMP-9 (180, 130, 92 kDa) or total MMP-9 at baseline. No differences were observed in TIMP-1 levels. In controls, decreased levels of TIMP-2 and the higher molecular forms of MMP-9 (180, 130, 92 kDa) were found 3 months after therapy compared with baseline (p < 0.01). Conclusions Higher levels of MMP-8, TIMP-2, MPO, and 87 kDa MMP-9 were found in the GCF of patients compared with controls...

‣ Haplotypes of susceptibility to chronic periodontitis in the Interleukin 8 gene do not influence protein level in the gingival crevicular fluid

Corbi, Samia C. T.; Anovazzi, Giovana; Finoti, Livia S.; Kim, Yeon J.; Capela, Marisa V.; Secolin, Rodrigo; Marcaccini, Andrea M.; Gerlach, Raquel F.; Orrico, Silvana R. P.; Cirelli, Joni A.; Scarel-Caminaga, Raquel M.
Fonte: Pergamon-Elsevier Science Ltd; Oxford Publicador: Pergamon-Elsevier Science Ltd; Oxford
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
100.02445%
Objective: Previously, we identified that the ATC/TTC haplotype formed by polymorphisms in the Interleukin-(IL)8 gene conferred susceptibility to chronic periodontitis (CP). The aim of the study was to investigate whether the IL8 haplotype ATC/TTC was associated with the volume of gingival crevicular fluid (GCF), the concentration of interleukin IL-8 in the GCF, as well as periodontal conditions in patients with CP in comparison to controls without CP. Methods: Seventy-nine individuals (CP: n = 41, controls: n = 38) were grouped according to the presence (susceptible for CP) or absence (not susceptible for CP) of the IL8 ATC/TTC haplotype. After periodontal clinical evaluation, they were subdivided by the presence or absence of CP. GCF was collected from each patient and the IL-8 levels were determined by ELISA. The GCF volume of each subject was measured by means of a calibrated electronic device. Comparisons of means between carriers and non-carriers of the ATC/TTC haplotype were evaluated using the Mann-Whitney test. Linear regression and stepwise linear regression analysis were used to analyse the association of the GCF volume with potential covariates and their contribution for the phenotype. Results: We did not find significant differences of both periodontal conditions and IL-8 concentration in the GCF of patients with the presence or absence of the IL8 ATC/TTC haplotype. However...

‣ Juveniles versus adults: differences in PGE2 levels in the gingival crevicular fluid during orthodontic tooth movement

Chibebe,Priscilla Campanatti; Starobinas,Nancy; Pallos,Debora
Fonte: Sociedade Brasileira de Pesquisa Odontológica - SBPqO Publicador: Sociedade Brasileira de Pesquisa Odontológica - SBPqO
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2010 Português
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This study aimed to investigate age-related changes in the biosynthetic capacity of prostaglandin E2 (PGE2) in the gingival crevicular fluid (GCF) during one month of orthodontic treatment. Twenty-five juvenile subjects (mean age 13 ± 2.1 years) and 23 adults (mean age 24 ± 2.1 years) were included. GCF was collected immediately before the force application at the baseline, 2, 21 and 28 days, with periopaper inserted into the gingival crevice of the maxillary lateral incisors. The mediator levels were determined with an EIA kit. The results showed that the PGE2 concentrations were significantly elevated from the baseline to 21 days (129.35 and 198.84 pg/µL, p = 0.0169) in juvenile subjects and reduced from 21 to 28 days (198.84 to 112.60 pg/µL, p = 0.0032). Adults, however, had no significant changes in the PGE2 levels. The total amounts of PGE2 from both groups changed between the baseline to 21 and 21 to 28 days (p = 0.0119 and p = 0.0076, respectively). The PGE2 initial and final levels showed significant differences between the juveniles and adults, being higher in adults (baseline: juvenile = 129.35 pg/µL vs. adult = 163.20 pg/µL, p = 0.0379; t3: juvenile = 112.60 pg/µL and adult = 175.30 pg/µL, p = 0.0005). In conclusion...

‣ Gingival crevicular fluid levels of clindamycin compared with its minimal inhibitory concentrations for periodontal bacteria.

Walker, C B; Gordon, J M; Cornwall, H A; Murphy, J C; Socransky, S S
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /05/1981 Português
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Clindamycin concentrations in gingival crevicular fluid and in blood were determined over a 7-h period and were related to the minimal inhibitory concentrations of this agent for 340 bacterial strains isolated from diseased periodontal sites. The clindamycin levels after administration of single 300-mg oral doses were measured in gingival crevicular fluids by using an agar diffusion bioassay. Minimal inhibitory concentrations were determined by agar dilution techniques for 30 species of periodontal bacteria. With the exception of Eikenella corrodens and Actinobacillus actinomycetemcomitans, most of the bacteria were inhibited by a concentration of 1.0 microgram of clindamycin per ml or less. The peak concentrations in crevicular fluid (2.0 +/- 0.3 microgram/ml) and in blood (1.9 +/- 0.3 micrograms/ml) were approximately the same. However, crevicular fluid levels of 1.0 micrograms/ml and above were present for up to 6 h, whereas blood concentrations dropped below 1.0 micrograms/ml within 2 h after administration. Based on its minimal inhibitory concentrations, clindamycin at crevicular fluid levels of 1.0 micrograms/ml or above should inhibit most bacteria associated with diseased periodontal sites.

‣ Partial characterization of an interleukin-1-like factor in human gingival crevicular fluid from patients with chronic inflammatory periodontal disease.

Kabashima, H; Maeda, K; Iwamoto, Y; Hirofuji, T; Yoneda, M; Yamashita, K; Aono, M
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /08/1990 Português
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A significant level of interleukin-1-(IL-1)-like activity was detected in gingival crevicular fluid obtained from sites in patients with chronic inflammatory periodontal disease, confirming a previous report of IL-1-like activity detected in human gingival crevicular fluid from patients with chronic inflammatory periodontitis (J. A. Charon, T. A. Luger, S. E. Mergenhagen, and J. J. Oppenheim, Infect. Immun. 38:1190-1195, 1982). In the present study, we sought to investigate whether this IL-1-like activity belonged to IL-1 alpha or IL-1 beta and to characterize some of the biochemical properties of this factor. Polyclonal antibodies against recombinant human IL-1 alpha or IL-1 beta (rIL-1 alpha or rIL-1 beta) have been used for serological comparison of the IL-1-like factor. IL-1-like activity was completely neutralized by anti-human rIL-1 alpha antiserum, but not by anti-human rIL-1 beta antiserum. On gel filtration with a high-pressure liquid chromatographic Superose 12 column, IL-1-like activity was separated into two peaks, one with a molecular weight of about 43,000 and the other with a molecular weight of less than 17,000. The majority of the IL-1-like factor with a low molecular weight in human gingival crevicular fluid migrated at a molecular weight of about 17...

‣ Gingival crevicular fluid a marker of the periodontal disease activity

Subrahmanyam, M. V.; Sangeetha, M.
Fonte: Springer India Publicador: Springer India
Tipo: Artigo de Revista Científica
Publicado em /01/2003 Português
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Gingival crevicular fluid (GCF) is an inflammatory exudate that can be collected at the gingival margin or within the gingival crevice. The biochemical analysis of the fluid offers a non invasive means of assessing the host response in periodontal disease. Active phase of periodontal disease process can be measured or assessed by the constituents of gingival fluid. Bacterial enzymes, bacterial degradation products, connective tissue degradation products, host mediated enzymes, inflammatory mediators, extracellular matrix proteins either together or individually can be detected in higher levels in gingival crevicular fluid during active phase of periodontitis.

‣ Salivary and gingival crevicular fluid histatin in periodontal health and disease

Bhadbhade, Smruti J.; Acharya, Anirudh B.; Thakur, Srinath L.
Fonte: Medicina Oral S.L. Publicador: Medicina Oral S.L.
Tipo: Artigo de Revista Científica
Publicado em 01/10/2013 Português
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Objectives: Histatin, with its anti bacterial, anti protease, and wound closure stimulating property might influence the pathogenesis of periodontal disease. This study assessed the presence of histatin in gingival crevicular fluid (GCF); the levels of salivary and GCF histatin in periodontal disease. Material and methods: It was a cross sectional study that included systemically healthy forty five subjects (22 males and 23 females) between the age group of 20 to 45 years. Based on Gingival Index (Loe and Silness ,1963) and Russell’s Periodontal Index they were grouped as 15 healthy (Group 1), 15 gingivitis (Group 2), and 15 periodontitis (Group 3) subjects. Whole pooled unstimulated saliva was collected by asking the patient to spit in a sterile container and GCF samples were collected using a micropipette from all the subjects. Histatin levels were assessed using Enzyme Linked Immunosorbent Assay (ELISA). The intergroup comparison was done by ANOVA and Mann Whitney U Test was done for pair wise comparison. Results: The results of this study show that histatin is present in saliva and gingival crevicular fluid. When the salivary histatin levels were compared it was found that the levels of histatin increase from health to periodontitis but the levels of histatin in the gingival crevicular fluid and saliva had no correlation with severity of periodontal disease as there was no statistically significant difference between the three groups. Conclusions: It can be concluded that histatin cannot be used as a potential marker of periodontal disease.

‣ Effect of smoking on concentrations of receptor activator of nuclear factor κ B ligand and osteoprotegerin in human gingival crevicular fluid; Effect of smoking on concentrations of receptor activator of nuclear factor kappa B ligand and osteoprotegerin in human gingival crevicular fluid

Tang, T.; Fitzsimmons, T.; Bartold, P.
Fonte: Blackwell Munksgaard Publicador: Blackwell Munksgaard
Tipo: Artigo de Revista Científica
Publicado em //2009 Português
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AIM: To compare the levels of the soluble receptor activator of nuclear factor kappa B ligand (sRANKL), osteoprotegerin (OPG) and their relative ratio in gingival crevicular fluid (GCF) among periodontitis patients with varying smoking histories. MATERIAL AND METHODS: GCF samples were collected from 149 periodontitis patients who were never smokers (n=58), former smokers (n=39) and current smokers (n=52). sRANKL and OPG concentrations in GCF were measured by enzyme-linked immunosorbent assays. RESULTS: sRANKL, OPG and their relative ratio were not statistically significant among the never smokers, former smokers and current smokers. However, OPG was significantly reduced and subsequently the sRANKL:OPG ratio was significantly increased in the high pack-years group as compared with never smokers. The positive correlation between pack-years and the sRANKL:OPG ratio remained statistically significant after adjusting for age and current smoking status. CONCLUSION: Increased lifetime exposure to cigarette smoking above a minimum threshold suppresses OPG production and leads to increased sRANKL:OPG. This may partially explain increased bone loss in smoking-related periodontitis.; Teck H. Tang, Tracy R. Fitzsimmons and P. Mark Bartold; © 2009 John Wiley & Sons

‣ Local and systemic biomarkers in gingival crevicular fluid increase odds of periodontitis

Fitzsimmons, T.; Sanders, A.; Bartold, P.; Slade, G.
Fonte: Blackwell Munksgaard Publicador: Blackwell Munksgaard
Tipo: Artigo de Revista Científica
Publicado em //2010 Português
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AIM: To determine the independent and combined associations of interleukin-1beta (IL-1beta) and C-reactive protein (CRP) in gingival crevicular fluid (GCF) on periodontitis case status in the Australian population. MATERIALS AND METHODS: GCF was collected from 939 subjects selected from the 2004-2006 Australian National Survey of Adult Oral Health: 430 cases had examiner-diagnosed periodontitis, and 509 controls did not. IL-1beta and CRP in GCF were detected by enzyme-linked immunosorbent assays. Odds ratios (OR) and 95% confidence intervals (CIs) were calculated in bivariate and stratified analysis and fully adjusted ORs were estimated using multivariate logistic regression. RESULTS: Greater odds of having periodontitis was associated with higher amounts of IL-1beta (OR=2.4, 95% CI=1.7-3.4 for highest tertile of IL-1beta relative to lowest tertile) and CRP (OR=1.9, 95% CI=1.5-2.5 for detectable CRP relative to undetectable CRP). In stratified analysis, there was no significant interaction between biomarkers (p=0.68). In the multivariate analyses that controlled for conventional periodontal risk factors, these relationships remained (IL-1beta OR=1.8, 95% CI=1.1-2.6; CRP OR=1.7, 95% CI=1.3-2.3). CONCLUSIONS: Elevated odds of clinical periodontitis was associated independently with each biomarker. This suggests that people with elevated biomarkers indicative of either local (IL-1beta) or systemic (CRP) inflammation are more likely to suffer from periodontal disease.; Tracy R. Fitzsimmons...

‣ C-reactive protein in gingival crevicular fluid may be indicative of systemic inflammation

Megson, E.; Fitzsimmons, T.; Dharmapatni, A.; Bartold, P.
Fonte: Blackwell Munksgaard Publicador: Blackwell Munksgaard
Tipo: Artigo de Revista Científica
Publicado em //2010 Português
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Background and Aim: Periodontitis is associated with elevated C-reactive protein (CRP) in both serum and gingival crevicular fluid (GCF). Although the liver is the primary source of CRP, extra-hepatic production of CRP has been reported. This study aimed to determine whether CRP in GCF is produced locally in the gingivae. Materials and Methods: Gingivae and GCF were collected from non-periodontitis and periodontitis sites. Presence of CRP in gingivae was assessed by immunohistochemistry. CRP in GCF was measured using ELISA. Gene expression for CRP in gingivae was determined using real-time polymerase chain reaction. Results: CRP was found in both the gingivae and GCF. No gingivae had detectable amounts of CRP mRNA. Not all patients with periodontitis had detectable levels of CRP in the GCF. Some non-periodontitis patients had detectable levels of CRP in the GCF. Conclusion: CRP in the GCF appears to be of systemic origin, and therefore may be indicative of systemic inflammation from either a periodontal infection or inflammatory disease elsewhere. The correlation between levels of CRP in GCF and serum requires validation in future studies.; Emma Megson, Tracy Fitzsimmons, Kencana Dharmapatni and P. Mark Bartold

‣ Bráquetes convencionais e autoligados: detecção de micro-organismos na saliva e in situ, avaliação de parâmetros periodontais e quantificação de citocinas no fluido crevicular; Self-ligating and Conventional brackets: Microbial detection in the saliva and in situ, periodontal index evaluation and assessment of cytokines levels in the gingival crevicular fluid

Bergamo, Ana Zilda Nazar
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 25/07/2014 Português
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O aparelho ortodôntico promove alterações microbiológicas na cavidade bucal, em função da variedade de materiais sólidos e elásticos que possuem, os quais funcionam como áreas de retenção, levando a um acúmulo de biofilme e predispondo o hospedeiro à cárie dental e à doença periodontal. Os objetivos do presente estudo, in vivo, foram: 1) Avaliar, as alterações no índice de placa (PI), índice gengival (GI), índice de sangramento gengival (GBI) e no volume do fluido crevicular, em pacientes com 3 diferentes desenhos de bráquetes metálicos (autoligados e convencionais), a fim de verificar se o desenho dos bráquetes interfere no acúmulo de placa e na saúde gengival; 2) Estudar as alterações nos níveis de diferentes micro-organismos envolvidos direta ou indiretamente na cárie dental, na saliva e in situ, pré e pós-colagem de bráquetes convencionais e autoligados; 3) Avaliar os níveis salivares e in situ, de espécies de micro-organismos dos complexos roxo, verde, laranja e vermelho e de outras espécies, pré e pós-colagem de bráquetes convencionais e autoligados, por meio de sondas de DNA e 4) Quantificar citocinas pró-inflamatórias no fluido crevicular, antes e após a colagem dos diferentes bráquetes. A amostra foi constituída de 20 pacientes...

‣ Quantitation of malondialdehyde in gingival crevicular fluid by a high-performance liquid chromatography-based method

Bastos, Alliny Souza; Melo Loureiro, Ana Paula de; Oliveira, Tiago Franco de; Corbi, Samia Cruz Tfaile; Caminaga, Raquel Mantuaneli Scarel; Rossa Junior, Carlos; Orrico, Silvana R. P.
Fonte: ACADEMIC PRESS INC ELSEVIER SCIENCE; SAN DIEGO Publicador: ACADEMIC PRESS INC ELSEVIER SCIENCE; SAN DIEGO
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
100.02445%
Lipid peroxidation (LPO) has been associated with periodontal disease, and the evaluation of malondialdehyde (MDA) in the gingival crevicular fluid (GCF), an inflammatory exudate from the surrounding tissue of the periodontium, may be useful to clarify the role of LPO in the pathogenesis of periodontal disease. We describe the validation of a method to measure MDA in the GCF using high-performance liquid chromatography. MDA calibration curves were prepared with phosphate-buffered solution spiked with increasing known concentrations of MDA. Healthy and diseased GCF samples were collected from the same patient to avoid interindividual variability. MDA response was linear in the range measured, and excellent agreement was observed between added and detected concentrations of MDA. Samples' intra- and interday coefficients of variation were below 6.3% and 12.4%, respectively. The limit of quantitation (signal/noise = 5) was 0.03 mu M. When the validated method was applied to the GCF, excellent agreement was observed in the MDA quantitation from healthy and diseased sites, and diseased sites presented more MDA than healthy sites (P < 0.05). In this study, a validated method for MDA quantitation in GCF was established with satisfactory sensitivity...

‣ Associations Between Matrix Metalloproteinase-8 and -14 and Myeloperoxidase in Gingival Crevicular Fluid From Subjects With Progressive Chronic Periodontitis: A Longitudinal Study

Mäntylä, Päivi; Rivera, Oriana; Dezerega Piwonka, Andrea Patricia; Tervahartiala, Taina; Gamonal Aravena, Jorge Antonio; Hernández, Marcela
Fonte: American Academy of Periodontology Publicador: American Academy of Periodontology
Tipo: Artículo de revista
Português
Relevância na Pesquisa
100.02445%
Background: Matrix metalloproteinase (MMP)-8 is a central mediator in chronic periodontitis. MMP-8 can be activated by the cooperative action of otherMMPs such asMMP-14, reactive oxygen species, andmicrobial proteases. The aimof this study is to associate the levels, molecular forms, isoenzyme distribution, and degree of activation of MMP-8 and -14, myeloperoxidase (MPO), and tissue inhibitor of MMP (TIMP)-1 in gingival crevicular fluid (GCF) from patients with progressive periodontitis at baseline and after periodontal therapy. Methods: In this longitudinal study, GCF samples from active (n = 25) and inactive (n = 25) sites of subjects with periodontitis were screened at baseline for GCF levels of MMP-8 by immunofluorometric assay, of MMP-14 by specific activity assay, and of MPOandTIMP-1 by enzyme-linked immunosorbent assay.MMP-8 andMPOwere alsomeasuredafterperiodontal treatment. Molecular forms were determined by immuno-Western blot analyses and subjected to densitometric scanning and statistical analyses. Results: High MMP-8 and MPO levels and a strong MPO/ MMP-8 positive correlation were found in active and inactive sites at baseline. After treatment, decreases in MPO and MMP-8 were seen, except for active sites in which MMP-8 differences were not significant (P <0.05). Conclusions: We present initial data that show that GCF levels and associations between MPO and MMP-8 are related to progression episodes and treatment responses in patients with chronic periodontitis. Our results suggest an interaction between the MPO oxidative pathway and MMP-8 activation...

‣ Longitudinal analysis of metalloproteinases, tissue inhibitors of metalloproteinases and clinical parameters in gingival crevicular fluid from periodontitis-affected patients.

Melej, C.; Martínez, B.; Zaldívar, M.; Valenzuela, M. A.; Gamonal Aravena, Jorge Antonio; Puente, J.; Pozo, P.
Fonte: Blacwell Munksgaard Publicador: Blacwell Munksgaard
Tipo: Artículo de revista
Português
Relevância na Pesquisa
100.67277%
Background:  The aim of this work was to improve the assessment of the periodontal disease status through measurements of extracellular matrix metalloproteinases (MMPs) and their tissular inhibitors (TIMPs) in the gingival crevicular fluid from patients diagnosed with chronic periodontitis. Methods:  Gingival crevicular fluid samples from patients (n = 13) were taken from 60 sites initially, and from 51 and 41 sites, respectively, 3 and 6 months after scaling and root planing. Gingival crevicular fluid samples were also taken from healthy subjects (n = 11, 24 sites). The presence of MMP-9 and MMP-8 was assessed by zymography and immunowestern blotting, respectively. The actual MMP activity (gelatinase and collagenase) was measured using the fluorogenic substrate assay. TIMP-1 and -2 levels were measured by immunodot blot. Results:  The fluorogenic substrate assay determinations showed higher MMP activity in sites with probing depth ≥ 4 mm, with significant reduction post-treatment. Gelatinase activity followed by zymography consisted mainly of MMP-9. A different pattern of MMP-8 in control and patient sites was found. Controls only showed species of a partially active form (69 kDa), whereas patient sites showed a high frequency of the active form (56 kDa)...