Página 1 dos resultados de 5740 itens digitais encontrados em 0.042 segundos

‣ Tratamento do papiloma vírus humano na infância com creme de imiquimode a 5%; Treatment of human papillomavirus in childhood with imiquimod 5% cream

BRANDT, Hebert Roberto Clivati; FERNANDES, Juliana Dumêt; PATRIOTA, Regia Celli Ribeiro; CRIADO, Paulo Ricardo; BELDA JUNIOR, Walter
Fonte: Sociedade Brasileira de Dermatologia Publicador: Sociedade Brasileira de Dermatologia
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
67.6549%
Lesões decorrentes da infecção pelo papilomavírus humano na infância, em especial as verrugas anogenitais, são um importante problema epidemiológico e terapêutico. O tratamento das verrugas anogenitais na infância é um desafio terapêutico. Os tratamentos convencionais geralmente são dolorosos e necessitam de anestesia geral. O imiquimode, um imunomodulador tópico, constitui uma alternativa terapêutica. Serão descritos quatro casos tratados com sucesso utilizando creme de imiquimode a 5% aplicado topicamente.; In children, lesions caused by the human papillomavirus (HPV) constitute a significant epidemiological issue and a therapeutic dilemma, particularly in the case of anogenital warts. The treatment of anogenital warts in children is a challenge, since standard treatments are generally painful and require the patient to be anesthetized. Imiquimod, a topical immune response modifier, constitutes an alternative therapeutic agent for the treatment of HPV. The present report describes four cases in which treatment with topically applied imiquimod 5% cream was implemented with successful results.

‣ Low human papillomavirus prevalence in head and neck cancer: results from two large case-control studies in high-incidence regions

RIBEIRO, Karina Braga; LEVI, Jose Eduardo; PAWLITA, Michael; KOIFMAN, Sergio; MATOS, Elena; ELUF-NETO, Jose; WUNSCH-FILHO, Victor; CURADO, Maria Paula; SHANGINA, Oxana; ZARIDZE, David; SZESZENIA-DABROWSKA, Neonila; LISSOWSKA, Jolanta; DAUDT, Alexander; ME
Fonte: OXFORD UNIV PRESS Publicador: OXFORD UNIV PRESS
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
67.42973%
Background Recent studies support an important role for human papillomavirus (HPV) in a subgroup of head and neck squamous cell carcinomas (HNSCC). We have evaluated the HPV deoxyribonucleic acid (DNA) prevalence as well as the association between serological response to HPV infection and HNSCC in two distinct populations from Central Europe (CE) and Latin America (LA). Methods Cases (n = 2214) and controls (n = 3319) were recruited from 1998 to 2003, using a similar protocol including questionnaire and blood sample collection. Tumour DNA from 196 fresh tissue biopsies was analysed for multiple HPV types followed by an HPV type-specific polymerase chain reaction (PCR) protocol towards the E7 gene from HPV 16. Using multiplex serology, serum samples were analysed for antibodies to 17 HPV types. Statistical analysis included the estimation of adjusted odds ratios (ORs) and the respective 95% confidence intervals (CIs). Results HPV16 E7 DNA prevalence among cases was 3.1% (6/196), including 4.4% in the oropharynx (3/68), 3.8% in the hypopharynx/larynx (3/78) and 0% among 50 cases of oral cavity carcinomas. Positivity for both HPV16 E6 and E7 antibodies was associated with a very high risk of oropharyngeal cancer (OR = 179, 95% CI 35.8-899) and hypopharyngeal/laryngeal cancer (OR = 14.9...

‣ Treatment of human papillomavirus in childhood with imiquimod 5% cream

BRANDT, Hebert Roberto Clivati; FERNANDES, Juliana Dumet; PATRIOTA, Regia Celli Ribeiro; CRIADO, Paulo Ricardo; BELDA JUNIOR, Walter
Fonte: SOC BRASILEIRA DERMATOLOGIA Publicador: SOC BRASILEIRA DERMATOLOGIA
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
67.51326%
In children, lesions caused by the human papillomavirus (HPV) constitute a significant epidemiological issue and a therapeutic dilemma, particularly in the case of anogenital warts. The treatment of anogenital warts in children is a challenge, since standard treatments are generally painful and require the patient to be anesthetized. Imiquimod, a topical immune response modifier, constitutes an alternative therapeutic agent for the treatment of HPV. The present report describes four cases in which treatment with topically applied imiquimod 5% cream was implemented with successful results.

‣ GP5+/6+ SYBR Green methodology for simultaneous screening and quantification of human papillomavirus

ARAUJO, Melissa Rodrigues de; MARCO, Laura De; SANTOS, Carlos F.; RUBIRA-BULLEN, Izabel Regina Fisher; RONCO, Guglielmo; PENNINI, Ilenia; VIZZINI, Loredana; MERLETTI, Franco; GILLIO-TOS, Anna
Fonte: ELSEVIER SCIENCE BV Publicador: ELSEVIER SCIENCE BV
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
67.42973%
Background: Detection and quantification of human papillomavirus (HPV) may help in predicting the evolution of HPV infection and progression of associated lesions. Objectives: We propose a novel protocol using consensus primers GP5+/6+ in a SYBR Green quantitative real-time (Q-RT) polymerase chain reaction (PCR). The strategy permits screening for HPV infection and viral load quantification simultaneously. Study design: DNA from 153 archived cervical samples, previously tested for HPV detection by GP5+/6+ PCR and typed by EIA-RLB (enzyme immunoassay-reverse line blot) or sequence analysis, was analysed using SYBR Green Q-RT PCR. Melting temperature assay (T(m)) and cycle threshold (C(t)) were used to evaluate HPV positivity and viral load. The T(m) in the range of 77-82 degrees C was considered to be positive for HPV-DNA. HPV results generated through GP5+/6+ conventional PCR were considered the gold standard against which sensitivity and specificity of our assay were measured. Results: Out of 104 HPV positive samples, 100 (96.2%) were also determined as positive by SYBR Green Q-RT PCR; of the 49 HPV-negative samples, all were determined as negative. There was an excellent positivity agreement (K = 0.94) between the SYBR Green Q-RT and the previous methods employed. The specificity and sensitivity were 100% and 96.2%...

‣ Estudo in vitro sobre a interação celular e vias endocíticas de papilomavírus humano (HPV) em leucócitos do sangue periférico.; In vitro study on the interaction of human papillomavirus in cell from peripheral blood leukocytes.

Szulczewski, Vívian
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 05/05/2009 Português
Relevância na Pesquisa
67.59047%
O papilomavírus humano (HPV) é o principal agente etiológico do câncer cervical e anogenital, sendo o HPV16 e o HPV18 os vírus de alto risco. Estudos recentes evidenciaram que além da transmissão sexual do HPV, há outras formas de contágio. Entretanto, a dificuldade na obtenção de quantidades viáveis do tipo selvagem ou mutante do HPV tem limitado em muito os estudos de diversos aspectos da biologia do papilomavírus. Este estudo investigou a possibilidade de o HPV infectar células leucocitárias do sangue periférico humano. Concluímos que as VLPs L1L2 do HPV16 podem utilizar a via endocítica do ferro mediada por clatrina, através do complexo VLPs-Transferrina-Receptor de Transferrina, permanecendo de forma latente em leucócitos. Esta porta de entrada oportunista poderia explicar a propagação crescente e alarmante deste agravo à saúde humana, motivo de preocupação nos sistemas mundiais de saúde pública. Este trabalho demonstrou pela primeira vez a internalização de VLPs L1L2 do HPV16 em leucócitos do sangue periférico humano.; Human papillomavirus (HPV) is the primary etiologic agent of anogenital and cervical cancer, caused mainly by the high-risk HPV16 and HPV18 viruses. Recent studies revealed that besides the sexual transmission of HPV...

‣ Perfil sociodemográfico e clínico de mulheres com infecção genital pelo Papilomavirus humano atendidas em um hospital de referência do interior paulista; The sociodemographic and clinical profile of women with genital human papillomavirus infection followed at a reference hospital in upstate São Paulo

Gaspar, Joice
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 28/06/2013 Português
Relevância na Pesquisa
67.379487%
Introdução: A infecção pelo Papilomavirus humano (HPV) enquadra-se como um problema de saúde pública, sendo considerada a infecção sexualmente transmissível (IST) mais comum. Estima-se, mundialmente, que aproximadamente 600 milhões de pessoas possuam o vírus do HPV e que em torno de 75 a 80% das pessoas adquiram- no em algum momento da vida. Objetivo: Analisar os aspectos sociodemográficos e clínicos em mulheres com infecção genital pelo HPV associando-os com o tipo de lesão genital e a sorologia reagente ou não reagente para o HIV e avaliar o seguimento clínico de mulheres com lesão intraepitelial de alto grau (HSIL) causada pelo HPV, que foram submetidas à cirurgia de alta frequência (CAF). Metodologia: Estudo transversal, retrospectivo, com abordagem quantitativa, realizado no Serviço de Moléstias Infecciosas em Ginecologia e Obstetrícia (SEMIGO) do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (HCFMRP- USP). Foram estudadas 1027 mulheres com diagnóstico de infecção genital pelo HPV, nas suas expressões lesão intraepitelial de baixo grau (LSIL), HSIL, condilomatoses (vulvar, vaginal, cervical e perianal), ou por identificação de sequências de DNA- HPV por meio de técnicas de biologia molecular. Foram elaborados dois formulários estruturados especificamente para este estudo...

‣ Correlação colpo-cito-histológica da infecção pelo papilomavírus humano em adolescentes com atividade sexual; Colposcopic, cytologic and histologic findings correlation with human papillomavirus infection in sexually active adolescents

Tubaki, Márcia Emy
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 04/05/2004 Português
Relevância na Pesquisa
67.42973%
Este estudo prospectivo foi delineado com o objetivo de avaliar os achados citológicos, colposcópicos e histológicos da infecção pelo papilomavírus humano em adolescentes e estabelecer a correlação entre o resultado da colpocitologia oncológica e os fatores de risco para a infecção pelo papilomavírus humano. O grupo foi constituído por 82 adolescentes entre 13 e 19 anos, acompanhadas no Ambulatório de Adolescentes e encaminhadas ao Setor de Patologia do Trato Genital Inferior e Colposcopia da Clínica Ginecológica do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo. As pacientes foram submetidas a anamnese, coleta da colpocitologia oncológica, colposcopia e biópsia de colo uterino na presença de aspectos colposcópicos anormais. A média etária das pacientes foi de 17,7 anos, com desvio-padrão de 1,4 anos. A colpocitologia oncológica foi normal em 57% e alterada em 43%. Os achados citológicos anormais diagnosticados foram: lesão intra-epitelial escamosa de baixo grau em 38%, lesão intra-epitelial escamosa de alto grau em 4% e células escamosas atípicas de significado indeterminado em 1%. A colposcopia foi satisfatória em todos os casos. Os achados colposcópicos normais foram o epitélio pavimentoso original em 4%...

‣ Computer-assisted analysis of p53 and PCNA expression in oral lesions infected with human papillomavirus

Soares, Christiane Pienna; Neto, Carlos Benatti; Fregonezi, Paula Andrea Gabrielli; Teresa, Debora Barreto; Santos, Raimunda Telma de Macedo; Longatto Filho, Adhemar; Maeda, Marina Yoshiê Sakamoto
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 19-24
Português
Relevância na Pesquisa
67.746655%
OBJECTIVE: To carry out a retrospective study to determine whether human papillomavirus (HPV) infection and immunohistochemical expression of p53 and proliferating cell nuclear antigen (PCNA) are related to the risk of oral cancer. STUDY DESIGN: Fifty-seven oral biopsies, consisting of 30 oral squamous papillomas (OSPs) and 27 oral squamous cell carcinomas (OSCCs) were tested for the presence of HPV 6/11 and 16/18 by in situ hybridization using catalyzed signal amplification and in situ hybridization. p53 And PCNA expression was analyzed by immunohistochemistry and evaluated quantitatively by image analysis. RESULTS: Nineteen of the 57 oral lesions (33.3%) were positive for HPV. HPV 6/11 was found in 6 of 30 (20%) OSPs and 1 of 27 (3.7%) OSCCs. HPV 16/18 was found in 10 of 27 (37%) OSCCs and 2 of 30 (6.7%) OSPs. Sixteen of the 19 HPV-positive cases (84.2%) were p53 negative; 5 (9%) were HPV 6/11 and 11 (19%) HPV 16/18, with an inverse correlation between the presence of HPV DNA and p53 expression (P=.017, P < .05). PCNA expression appeared in 18 (94.7%) of HPV positive cases, showing that HPV 16/18 was associated with intensity of PCNA expression and with OSCCs (P=.037, P < .05). CONCLUSION: Quantitative evaluation of p53 by image analysis showed an inverse correlation between p53 expression and HPV presence...

‣ p16INK4A immunohistochemical overexpression in premalignant and malignant oral lesions infected with human papillomavirus

Fregonesi, Paula Andrea Gabrielli; Teresa, Debora Barreto; Duarte, Roberta Aparecida; Neto, Carlos Benatti; De Oliveira, Maria Rita Brancini; Soares, Christiane Pienna
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 1291-1297
Português
Relevância na Pesquisa
67.69641%
Human papillomavirus (HPV) is believed to promote the oncogenic process, and the correlation between viral oncoproteins and dysfunction of p16 INK4A tumor suppressor protein in oral lesions is controversial. To test the hypothesis that anogenital HPV types participate in disruption of the regulation of p16INK4A suppressor protein in oral lesions, we analyzed 46 oral biopsy specimens for the presence of HPV 6/11 and 16/18 by in situ hybridization (ISH) and for p16INK4A expression by immunohistochemistry (IHC). Eighteen (39%) of the 46 oral lesions were HPV-positive and 28 (61%) were HPV-negative. HPV 6/11 DNA was found in 5 (11%) and HPV 16/18 in 13 (28%) of 46 biopsies. Nine of the 18 HPV-positive oral lesions (50%), assessed by catalyzed signal amplification coupled to ISH (CSA-ISH), gave high-intensity p16INK4A immunostaining. Focal and diffuse patterns were observed in 11/13 (77%) lesions with HPV 16/18, focal immunopositivity in 3/5 (80%) with HPV 6/11, and negative or sporadic p16-labeling in 18/28 (64%) without the presence of HPV DNA. These results showed a strong association between overexpression of p16 protein and malignant oral lesions, mainly those infected by HPV 16/18. We can conclude that high-risk HPV types are associated with p16 overexpression...

‣ Human papillomavirus and Epstein-Barr virus infection, p53 expression, and cellular proliferation in laryngeal carcinoma

Elgui de Oliveira, Deilson; Bacchi, Maura M.; Macarenco, Ricardo S.S.; Tagliarini, José Vicente; Cordeiro, Ricardo C.; Bacchi, Carlos E.
Fonte: Universidade Estadual Paulista Publicador: Universidade Estadual Paulista
Tipo: Artigo de Revista Científica Formato: 284-293
Português
Relevância na Pesquisa
67.46377%
Laryngeal carcinomas are aggressive neoplasms with controversial association with the human papillomavirus (HPV) and Epstein-Barr virus (EBV). So far, the impairment of p53 protein function and its impact on cellular proliferation has not been studied adequately in these tumors. In this work, molecular biologic techniques were used to assess the frequency of HPV and EBV in 110 squamous cell carcinomas of the larynx. In addition, accumulation of p53 and Ki-67 cell proliferation antigen expression in malignant cells was assessed by immunohistochemical analysis. High-grade HPV was found in 37.3% of cases, and none had demonstrable EBV infection. Accumulation of p53 was found in 78.2% of the cases, and it was related to a high Ki-67 labeling index and higher histologic grade. The results demonstrate association of HPV with more than one third of laryngeal carcinomas studied, mainly glottic tumors. Tumors with increased cell proliferation were more frequently high grade, with p53 accumulation and lymph node metastasis. © American Society for Clinical Pathology.

‣ Treatment of human papillomavirus in childhood with imiquimod 5% cream

BRANDT, Hebert Roberto Clivati; FERNANDES, Juliana Dumet; PATRIOTA, Regia Celli Ribeiro; CRIADO, Paulo Ricardo; BELDA JUNIOR, Walter
Fonte: SOC BRASILEIRA DERMATOLOGIA Publicador: SOC BRASILEIRA DERMATOLOGIA
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
67.51326%
In children, lesions caused by the human papillomavirus (HPV) constitute a significant epidemiological issue and a therapeutic dilemma, particularly in the case of anogenital warts. The treatment of anogenital warts in children is a challenge, since standard treatments are generally painful and require the patient to be anesthetized. Imiquimod, a topical immune response modifier, constitutes an alternative therapeutic agent for the treatment of HPV. The present report describes four cases in which treatment with topically applied imiquimod 5% cream was implemented with successful results.

‣ Epidemiological and functional implications of molecular variants of human papillomavirus

Sichero,L.; Villa,L.L.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2006 Português
Relevância na Pesquisa
67.42973%
Human papillomavirus genomes are classified into molecular variants when they present more than 98% of similarity to the prototype sequence within the L1 gene. Comparative nucleotide sequence analyses of these viruses have elucidated some features of their phylogenetic relationship. In addition, human papillomavirus intratype variability has also been used as an important tool in epidemiological studies of viral transmission, persistence and progression to clinically relevant cervical lesions. Until the present, little has been published concerning the functional significance of molecular variants. It has been shown that nucleotide variability within the long control region leads to differences in the binding affinity of some cellular transcriptional factors and to the enhancement of the expression of E6 and E7 oncogenes. Furthermore, in vivo and in vitro studies revealed differences in E6 and E7 biochemical and biological properties among molecular variants. Nevertheless, further correlation with additional functional information is needed to evaluate the significance of genome intratypic variability. These results are also important for the development of vaccines and to determine the extent to which immunization with L1 virus-like particles of one variant could induce antibodies that cross-neutralize other variants.

‣ Prevalence of high-risk human papillomavirus by cobas 4800 HPV test in urban Peru

Iwasaki,Ricardo; Galvez-Philpott,Felipe; Arias-Stella Jr.,Javier; Arias-Stella,Javier
Fonte: Brazilian Society of Infectious Diseases Publicador: Brazilian Society of Infectious Diseases
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2014 Português
Relevância na Pesquisa
67.820225%
Background: Molecular tests allow the detection of high-risk human papillomavirus in cervical samples, playing an important role in the prevention of cervical cancer. Objectives: We performed a study to determine the prevalence of HPV 16, HPV 18 and other high-risk human papillomavirus (pool 12 genotypes) in Peruvian females from diverse urban areas using the cobas 4800 HPV test. Methods: Routine cervical samples collected in our laboratory were analyzed by cobas 4800 HPV test. Results: A total of 2247 samples from female patients aged 17–79 years were tested. high-risk human papillomavirus was positive in 775 (34.49%) samples. Of these, 641 (82.71%) were single infections and 134 (17.29%) were multiple infections. The positivity rates for HPV 16, HPV 18, and other high-risk human papillomavirus were 10.77%, 2.0%, and 28.08%, respectively. In multiple high-risk human papillomavirus infections, the concomitance of HPV 16 and other high-risk human papillomavirus was more prevalent (13.42%). Conclusion: Our study showed high prevalence of high-risk human papillomavirus in urban Peru, mainly among young women. In both single and multiple infections other high-risk human papillomavirus were more prevalent than HPV 16 and HPV 18...

‣ Phylogenetic classification of human papillomavirus genotypes in high-grade cervical intraepithelial neoplasia in women from a densely populated Brazilian urban region

Pitta,Denise Rocha; Sarian,Luis Otávio; Campos,Elisabete Aparecida; Rabelo-Santos,Sílvia Helena; Syrjänen,Kari; Derchain,Sophie Françoise
Fonte: Associação Paulista de Medicina - APM Publicador: Associação Paulista de Medicina - APM
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/01/2009 Português
Relevância na Pesquisa
67.63221%
CONTEXT AND OBJECTIVE: Differences in human papillomavirus (HPV) types may correlate with the biological potential and invasion risk of high-grade cervical intraepithelial neoplasia (CIN 2 and CIN 3). The objective of this study was to determine the relationship between different combinations of HPV types and CIN severity. DESIGN AND SETTING: Cross-sectional study, at Universidade Estadual de Campinas (Unicamp). METHODS: Cervical samples from 106 women treated due to CIN 2 (18) or CIN 3 (88) were examined for specific HPV genotypes using Roche Linear Array® (LA-HPV). The proportions of CIN 2 and CIN 3 in groups of women infected with the HPV phylogenetic groups A7 and A9 were compared. Three groups were formed: women with single infections; multiple infections; and the whole sample. RESULTS: Multiple infections were detected in 68 samples (64.7%). The most frequent high-risk genotypes detected (single/multiple) were HPV 16 (57.1%), HPV 58 (24.7%), HPV 33 (15.2%), HPV 52 (13.3%), HPV 31 (10.4%), HPV 51 (7.6%) and HPV 18 (6.6%). Women without infection with HPV species Alpha 9 were less likely to have CIN 3 than were their Alpha 9 HPV-infected counterparts. HPV 16 and/or HPV 18, with or without associations with other viral types, were more frequently found in women with CIN 3 than in those with CIN 2. CONCLUSIONS: The severity of high-grade CIN may be aggravated by the presence of HPV types included in the Alpha 9 phylogenetic classification and by infections including HPV 16 and 18...

‣ The impact of glucocorticoids and anti-cd20 therapy on cervical human papillomavirus infection risk in women with systemic lupus erythematosus

Mendoza-Pinto,Claudia; Garcia-Carrasco,Mario; Vallejo-Ruiz,Veronica; Taboada-Cole,Alejandro; Munoz-Guarneros,Margarita; Solis-Poblano,Juan Carlos; Pezzat-Said,Elias; Aguilar-Lemarroy,Adriana; Jave-Suarez,Luis Felipe; de Lara,Luis Vazquez; Ramos-Alvarez,Gl
Fonte: Faculdade de Medicina / USP Publicador: Faculdade de Medicina / USP
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2013 Português
Relevância na Pesquisa
67.871504%
OBJECTIVE: To identify the prevalence and factors associated with cervical human papillomavirus infection in women with systemic lupus erythematosus METHODS: This cross-sectional study collected traditional and systemic lupus erythematosus-related disease risk factors, including conventional and biologic therapies. A gynecological evaluation and cervical cytology screen were performed. Human papillomavirus detection and genotyping were undertaken by PCR and linear array assay. RESULTS: A total of 148 patients were included, with a mean age and disease duration of 42.5±11.8 years and 9.7±5.3 years, respectively. The prevalence of squamous intraepithelial lesions was 6.8%. The prevalence of human papillomavirus infection was 29%, with human papillomavirus subtype 59 being the most frequent. Patients with human papillomavirus were younger than those without the infection (38.2±11.2 vs. 44.2±11.5 years, respectively; p = 0.05), and patients with the virus had higher daily prednisone doses (12.8±6.8 vs. 9.7±6.7 mg, respectively; p = 0.01) and cumulative glucocorticoid doses (14.2±9.8 vs. 9.7±7.3 g, respectively; p = 0.005) compared with patients without. Patients with human papillomavirus infection more frequently received rituximab than those without (20.9% vs. 8.5%...

‣ Clinicopathological aspects and prevalence of human papillomavirus in anal cancer

Aguiar,Marina Tayla Mesquita; Bosso,Natália Carelli de Castro; Leal,Caio Bruno Quinta de Souza; Lira,Clerlhan Ferreira de; Cabral,Lázara Alyne Oliveira; Silva,Antonio Márcio Teodoro Cordeiro; Saddi,Vera Aparecida
Fonte: Sociedade Brasileira de Coloproctologia Publicador: Sociedade Brasileira de Coloproctologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2014 Português
Relevância na Pesquisa
67.75835%
Anal cancer is relatively rare; however, its incidence has increased in recent years. Several risk factors are associated with the development of anal cancer, including age older than 50 years, low-fiber diet, chronic anal fistulas, smoking, multiple partners, anal intercourse practice, Human Immunodeficiency Virus infection and immunosuppression. However, the presence of human papillomavirus represents the main risk factor for the development of anal cancer. The aim of this study was to evaluate the clinicopathological aspects of a series of patients with anal carcinomas diagnosed in Hospital Araújo Jorge, Goiânia-Goiás, as well as the prevalence of human papillomavirus genome in these tumors. Clinical, pathological and socio-demographic data were collected from the respective medical files and paraffin blocks containing anal carcinomas specimens were used for DNA extraction and detection of human papillomavirus, by means of polymerase chain reaction, using short PCR fragment primers. Forty-three cases were selected and had the data analyzed, while 38 cases were tested for human papillomavirus genome detection. Among the evaluated patients, 62.8% were women; 53.4% of tumors were squamous cell carcinoma and 46.5% of the patients were aged between 60 and 75 years. Risk factors...

‣ Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection

Padovan, Cacilda Tezelli Junqueira; Bonin, Camila Mareti; Tozetti, Inês Aparecida; Ferreira, Alda Maria Teixeira; Fernandes, Carlos Eurico dos Santos; Costa, Izaias Pereira da
Fonte: Revista da Sociedade Brasileira de Medicina Tropical Publicador: Revista da Sociedade Brasileira de Medicina Tropical
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
67.68894%
ABSTRACT - Introduction The progression of human papillomavirus (HPV) infection in the anogenital tract has been associated with the involvement of cells with regulatory properties. Evidence has shown that glucocorticoid-induced tumor necrosis factor receptor (GITR) is an important surface molecule for the characterization of these cells and proposes that GITR ligand may constitute a rational treatment for many cancer types. We aimed to detect the presence of GITR and CD25 in cervical stroma cells with and without pathological changes or HPV infection to better understand the immune response in the infected tissue microenvironment. Methods We subjected 49 paraffin-embedded cervical tissue samples to HPV DNA detection and histopathological analysis, and subsequently immunohistochemistry to detect GITR and CD25 in lymphocytes. Results We observed that 76.9% of all samples with high GITR expression were HPV-positive regardless of histopathological findings. High GITR expression (77.8%) was predominant in samples with ≥1,000 RLU/PCB. Of the HPV-positive samples negative for intraepithelial lesion and malignancy, 62.5% had high GITR expression. High GITR expression was observed in both carcinoma and high-grade squamous intraepithelial lesion (HSIL) samples (p = 0.16). CD25 was present in great quantities in all samples. Conclusions The predominance of high GITR expression in samples with high viral load that were classified as HSIL and carcinoma suggests that GITR+ cells can exhibit regulatory properties and may contribute to the progression of HPV-induced cervical neoplasia...

‣ Entwicklung eines Produktions- und DNA-Verpackungssystems für Virus-ähnliche Partikel des humanpathogenen Papillomavirus Typ 16; Development of a production and DNA-packaging system for virus like particles of human papillomavirus type 16

Jung, Marcel-Alexander
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
67.42973%
Die weltweit verbreiteten Papillomaviren sind kleine unbehüllte DNA-Viren, die eine ausgeprägte Wirts- bzw. Gewebsspezifität besitzen und sich ausschließlich in den oberen Schichten von Haut- und Schleimhautepithelien vermehren. Die Infektion dieser Viren verläuft beim Menschen in der Regel inapparent, kann aber in Abhängigkeit vom Virustyp auch zur Entstehung der zweithäufigsten Krebsart bei Frauen - dem Zervixkarzinom - führen. Das Virusgenom wird von mehreren Kopien der zwei Strukturproteine L1 und L2 ein-geschlossen und bildet somit die infektiöse Einheit. Die genauen Abläufe beim Kapsidassemblierungs- und Verpackungsprozess sowie die Vorgänge bei der Auf-nahme und Prozessierung der Viren sind noch weitgehend ungeklärt. Die Möglichkeit zur künstlichen Herstellung dieser Viren ist eine wichtige Voraussetzung zur Erfor-schung der zuvor genannten Prozesse, bei denen große Mengen an infektiösem Vi-rusmaterial benötigt werden. Die Herstellung dieser Viren im Labor hat sich aber als kompliziert erwiesen, da die Expression der Strukturproteine sehr stark reguliert und eng mit dem Differenzierungsprogramm der Zelle verknüpft ist. Bis heute wurde eine Vielzahl an Produktionssystemen entwickelt, deren Nachteile v.a. durch geringe Ausbeuten...

‣ Time trends of human papillomavirus types in invasive cervical cancer, from 1940 to 2007

Laco, Jan; Guarch, Rosa; Bravo, Ignacio G.; Lerma, Enrique; Bosch, F. Xavier; Lloveras, Belén; Castellsagué, Xavier; Molina, Carla; Clavero, Omar; Muñoz, Nubia; Felix, Ana; Sánchez, Gloria I.; Andújar, Miguel; Ordi, Jaume; Velasco, Julio; Pelayo, Ade
Fonte: UICC Publicador: UICC
Tipo: Artículo de revista
Português
Relevância na Pesquisa
67.488354%
Artículo de publicación ISI; Contribution over time of human papillomavirus (HPV) types in human cancers has been poorly documented. Such data is fundamental to measure current HPV vaccines impact in the years to come. We estimated the HPV type-specific distribution in a large international series of invasive cervical cancer (ICC) over 70 years prior to vaccination. Paraffin embedded ICC cases diagnosed between 1940 and 2007 were retrieved from eleven countries in Central-South America, Asia and Europe. Included countries reported to have low-medium cervical cancer screening uptake. Information on age at and year of diagnosis was collected from medical records. After histological confirmation, HPV DNA detection was performed by SPF-10/DEIA/LiPA25 (version1). Logistic regression models were used for estimating the adjusted relative contributions (RC) of HPV16 and of HPV18 over time. Among 4,771 HPV DNA positive ICC cases, HPV16 and HPV18 were the two most common HPVs in all the decades with no statistically significant variations of their adjusted-RC from 1940–59 to 2000–07 (HPV16—from 61.5 to 62.1%, and HPV18—from 6.9 to 7.2%). As well, the RC of other HPV types did not varied over time. In the stratified analysis by histology...

‣ A polytope DNA vaccine elicits multiple effector and memory CTL responses and protects against human papillomavirus 16 E7-expressing tumour

Doan, Tracy; Herd, Karen; Ramshaw, Ian; Thomson, Scott; Tindle, Robert W
Fonte: Springer Publicador: Springer
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
67.46377%
Vaccine-induced CD8 T cells directed to tumour-specific antigens are recognised as important components of protective and therapeutic immunity against tumours. Where tumour antigens have pathogenic potential or where immunogenic epitopes are lost from tumours, development of subunit vaccines consisting of multiple individual epitopes is an attractive alternative to immunising with whole tumour antigen. In the present study we investigate the efficacy of two DNA-based multiepitope ('polytope') vaccines containing murine (H-2b) and human (HLA-A*0201)-restricted epitopes of the E7 oncoprotein of human papillomavirus type 16, in eliciting tumour-protective cytotoxic T-lymphocyte (CTL) responses. We show that the first of these polytopes elicited powerful effector CTL responses (measured by IFN-γ ELISpot) and long-lived memory CTL responses (measured by functional CTL assay and tetramers) in immunised mice. The responses could be boosted by immunisation with a recombinant vaccinia virus expressing the polytope. Responses induced by immunisation with polytope DNA alone partially protected against infection with recombinant vaccinia virus expressing the polytope. Complete protection was afforded against challenge with an E7-expressing tumour...