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‣ Reversible flow of cholesteryl ester between high-density lipoproteins and triacylglycerol-rich particles is modulated by the fatty acid composition and concentration of triacylglycerols

CAZITA, P.M.; CASTILHO, L.N.; CARVALHO, M.D.T.; SESSO, A.C.; OLIVEIRA, H.C.F.; QUINTÃO, E.C.R.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.900894%
We determined the influence of fasting (FAST) and feeding (FED) on cholesteryl ester (CE) flow between high-density lipoproteins (HDL) and plasma apoB-lipoprotein and triacylglycerol (TG)-rich emulsions (EM) prepared with TG-fatty acids (FAs). TG-FAs of varying chain lengths and degrees of unsaturation were tested in the presence of a plasma fraction at d > 1.21 g/mL as the source of CE transfer protein. The transfer of CE from HDL to FED was greater than to FAST TG-rich acceptor lipoproteins, 18% and 14%, respectively. However, percent CE transfer from HDL to apoB-containing lipoproteins was similar for FED and FAST HDL. The CE transfer from HDL to EM depended on the EM TG-FA chain length. Furthermore, the chain length of the monounsaturated TG-containing EM showed a significant positive correlation of the CE transfer from HDL to EM (r = 0.81, P < 0.0001) and a negative correlation from EM to HDL (r = -041, P = 0.0088). Regarding the degree of EM TG-FAs unsaturation, among EMs containing C18, the CE transfer was lower from HDL to C18:2 compared to C18:1 and C18:3, 17.7%, 20.7%, and 20%, respectively. However, the CE transfer from EMs to HDL was higher to C18:2 than to C18:1 and C18:3, 83.7%, 51.2%, and 46.3%, respectively. Thus...

‣ Influences of sex and age on biological rhythms of serum lipids and lipoproteins

DALPINO, Fabio B.; MENNA-BARRETO, Luiz; FARIA, Eliana C. de
Fonte: ELSEVIER SCIENCE BV Publicador: ELSEVIER SCIENCE BV
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.81316%
Background: Few studies have evaluated seasonal variations of biochemical parameters routinely analyzed in clinical laboratories. Rhythmic patterns for lipids and lipoproteins have been demonstrated and have been the object of research, mainly because of their demonstrated association with coronary artery disease. This study evaluated the occurrence of biological rhythms on serum lipids and lipoproteins and the effects of sex and age on the rhythms in a Brazilian hospital outpatient population. Methods: Retrospective laboratory study was carried out to evaluate the results of total cholesterol (TC), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C) and triglycerides (TG), from individuals registered at a university referral hospital over 8years. The studied population was composed of individuals of both sexes and all ages totaling 38,579 participants and 301,934 measurements. Statistical analyses were carried out using the SAS program and the temporal analysis used the Cosinor method. Results: TG rhythm was present only in females. All other parameters were equally rhythmic in both sexes. Regarding age, HDL-C presented rhythms in all age groups, but TC and LDL-C showed seasonality only for those > 13years, TG did not present rhythms in all age groups. Conclusion: Effects of sex and age on biological rhythms detected in TC...

‣ Cu and Fe metallic ions-mediated oxidation of low-density lipoproteins studied by NMR, TEM and Z-scan technique

GOMEZ, S. L.; MONTEIRO, A. M.; Rabbani, Said Rahnamaye; Bloise Junior, Antonio Carlos; CARNEIRO, S. M.; ALVES, S.; GIDLUND, M.; ABDALLA, D. S. P.; Figueiredo Neto, Antonio Martins
Fonte: ELSEVIER IRELAND LTD Publicador: ELSEVIER IRELAND LTD
Tipo: Artigo de Revista Científica
Português
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36.683647%
In this work we report on a study of the morphological changes of LDL induced in vitro by metallic ions (Cu(2+) and Fe(3+)). These modifications were characterized by transmission electron microscopy, nuclear magnetic resonance and the Z-scan technique. The degree of oxidative modification of LDL was determined by the TBARS and lipid hydroperoxides assays. It is shown that distinct pathways for modifying lipoproteins lead to different morphological transformations of the particles characterized by changes in size and/or shape of the resulting particles, and by the tendency to induce aggregation of the particles. There were no evidence of melting of particles promoted by oxidative processes with Cu and Fe. (C) 2010 Elsevier Ireland Ltd. All rights reserved.; State of Sao Paulo Research Foundation (FAPESP); National Institute of Science and Technology of Complex Fluids (INCT-FCx); National Counsel of Technological and Scientific Development (CNPq)

‣ Metabolism of triglyceride-rich lipoproteins and lipid transfer to high-density lipoprotein in young obese and normal-weight patients with polycystic ovary syndrome

ROCHA, Michelle P.; MARANHAO, Raul C.; SEYDELL, Talita M.; BARCELLOS, Cristiano R. G.; BARACAT, Edmundo C.; HAYASHIDA, Sylvia A. Y.; BYDLOWSKI, Sergio P.; MARCONDES, Jose A. M.
Fonte: ELSEVIER SCIENCE INC Publicador: ELSEVIER SCIENCE INC
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.683647%
Objective: To clarify whether the metabolism of triglyceride-rich lipoproteins and lipid transfer to high-density lipoprotein (HDL) are altered in patients with polycystic ovary syndrome (PCOS). Design: Case control study. Setting: Endocrinology clinics. Patient(s): Eight normal-weight (NW) and 15 obese (013) patients with PCOS were compared with 10 NW and 10 Ob women without PCOS paired for age and body mass index. Intervention(s): Determination of triglyceride-rich lipoprotein metabolism and lipid transfer to HDL. Main Outcome Measure(s): Participants were injected triglyceride-rich emulsions labeled with (14)C-cholesteryl esters and (3)H-triglycerides and the fractional clearance rate (FCR, in min(-1)) of labels was determined. Lipid transfer from artificial nanoemulsions to HDL was performed by incubating radioactively labeled lipid nanoemulsions with plasma during 1 hour, followed by radioactive counting of HDL-containing supernatant after chemical precipitation. Result(s): Lipolysis estimated by triglyceride FCR was equal in PCOS groups (NW = 0.043 +/- 0.032, Ob = 0.033 +/- 0.009) and respective controls (NW = 0.039 +/- 0.015, Ob = 0.044 +/- 0.019). However, the remnant removal as estimated by cholesteryl ester FCR was reduced in both PCOS groups (NW = 0.005 +/- 0.006...

‣ Influência do exercício físico nas lipoproteínas e no endotélio de pacientes com síndrome metabólica; Role of exercise in lipoproteins and in the endothelium of patients with metabolic syndrome

Casella Filho, Antonio
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 21/09/2007 Português
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A disfunção endotelial é um dos componentes básicos tanto da origem como das complicações de algumas doenças cardiovasculares, principalmente aquelas devidas a aterosclerose. Fatores de risco que compõe a Síndrome Metabólica (SMet) interferem na integridade endotelial por causarem marcante estresse oxidativo e conseqüente disfunção endotelial. Os benefícios de um treinamento físico de longa duração sobre o endotélio e sobre a concentração das lipoproteínas já são conhecidos. Entretanto, ainda restam lacunas de conhecimento dos efeitos que um treinamento de curta duração produziria em pacientes portadores de Síndrome Metabólica. Para elucidarmos este assunto, estudamos 40 indivíduos sedentários sendo 30 portadores de SMet e 10 normais para controle. Vinte dos pacientes com SMet (10 mulheres e 10 homens) realizaram treinamento físico (TF) aeróbio de moderada intensidade, em bicicleta ergométrica, por um período de 3 meses. A reatividade vascular e testes funcionais in vitro das lipoproteínas HDL e LDL foram realizados antes e depois do TF. Os resultados indicam melhora na unção endotelial, porém sem mudanças do índice de massa corpórea e dos níveis lipídicos. Houve redução da circunferência abdominal e dos níveis de Triglicérides. Os testes funcionais revelaram que...

‣ Seriam as apolipoproteinas A e B mais eficientes do que as lipoproteinas na investigacao de risco de doenca arterial coronariana obstrutiva?; Are apolipoproteins A and B better than lipoproteins for assessing risk of obstructive coronary heart disease?

Manfroi, Waldomiro Carlos; Zago, Alcides José; Alves, Alexandre; Brisolara, Maria Lucia; Souza, Josiane de; Candiago, Rafael Henriques; Kirschnick, Luciana Schmidt; Ribeiro, Leticia Carina; Ordovas, Karen Gomes; Leitão, Cristiane Bauermann; Cruz, Rosana
Fonte: Universidade Federal do Rio Grande do Sul Publicador: Universidade Federal do Rio Grande do Sul
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
Relevância na Pesquisa
36.900894%
Objective – To evaluate whether apolipoproteins A-I (Apo A-I) and B (Apo B) have, higher ensitivity (SN), specificity (SP) and positive predictive value (PPV) than lipoproteins (LP), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL), and triglycerides (TGL) in assessing the risk of coronary heart disease (CHD). Methods – This is a transversal case-control study of 241 patients, who were divided into two groups: 1) 145 patients with CHD, and 2) 96 patients without coronary disease. A model of logistic regression to evaluate the relation between the LPs and CHD was developed in which variables with a p-alpha <0.1 were included. Results – Apo A-I levels were higher in the patients without CHD, (OR 2.08, CI 1.20-3.57). There were no statistical differences between the values of Apo A-I and the remaining lipid fractions (Apo A-I: 67%; Apo B: 100%; PPV: TC= 71%; TGC=71%; HDL=71%; LDL=71%). The costs of the tests in Reais were as follows: Apo A-I: R$ 56.60; Apo B-100: R$ 56.60; TC: R$ 9.94; HDL: R$ 21.30; LDL: R$ 28.40; TGL: R$ 14.20. Conclusion – Levels of Apo A-I and Apo B have no advantage over conventional lipoproteins in predicting the risk of CHD, despite the statistical association between Apo A-I and CHD; in addition...

‣ Modulação funcional e genica de lipides e lipoproteinas plasmaticos e da aterosclerose carotidea na hiperalfalipoproteinemia; Functional and genic modulation of serum lipids and lipoproteins of carotid atherosclerosis in hyperalphalipoproteinemia

Fernanda Dutra Santiago
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 31/08/2007 Português
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Está bem estabelecida na literatura especializada a associação inversa entre as concentrações plasmáticas de colesterol das lipoproteínas de alta densidade (HDL-C) e a incidência de doença arterial coronariana (DAC). Além de propriedades anti-oxidante, anti-inflamatória e anti-trombótica, a HDL participa do transporte reverso de colesterol, via pela qual o colesterol é captado das lipoproteínas e das membranas células periféricas e transportado ao fígado para sua excreção na forma livre ou de ácidos biliares. A lipase hepática (LH) possui função crucial no transporte reverso do colesterol, por sua atividade lipolítica e pela função de ligante à lipoproteínas facilitando sua captação tissular. A proteína de transferência de ésteres de colesterol (CETP), e mesma importância metabólica, promove a troca de ésteres de colesterol por triglicérides entre a HDL e as lipoproteínas ricas em triglicérides. Mutações nos genes que codificam estas proteínas têm sido muito estudadas para se compreender a função destas no metabolismo lipídico. O modelo experimental da hiperalfalipoproteinemia tem sido utilizado no decorrer dos últimos anos com o intuito de elucidar os mecanismos de ação da HDL e das proteínas reguladoras do seu metabolismo. A hiperalfalipoproteinemia é caracterizada pelo aumento das concentrações de HDL-C e é causada principalmente por deficiências genética de CETP e/ou LH. Os objetivos desta dissertação foram o de se estabelecer à modulação da hiperalfalipoproteinemia sobre os parâmetros antropométricos...

‣ Modificações oxidativas de lipoproteínas de baixa densidade e seus anticorpos : associação com componentes do transporte reverso de colesterol e com a aterosclerose carotídea em adultos sem doença aterosclerótica estabelecida; Oxidative modification of plasma low density lipoproteins and their antibodies : association with the reverse cholesterol transport and carotid atherosclerosis in adults without established aterosclerotic disease

Paolla Fernanda Cezar Ferreira
Fonte: Biblioteca Digital da Unicamp Publicador: Biblioteca Digital da Unicamp
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 26/05/2011 Português
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36.683647%
A doença cardiovascular (DCV) é a causa mais comum de morte nas sociedades ocidentais, afetando ambos os sexos, sendo a aterosclerose a mais importante patologia subjacente. A aterosclerose é aceita mundialmente como uma doença imune-inflamatória, na qual, a participação do sistema imunológico é crucial para o início da disfunção endotelial, formação da estria gordurosa, progressão da lesão e ruptura da placa. A modificação oxidativa das lipoproteínas de baixa densidade (LDL), é considerada essencial no processo aterosclerótico, gerando LDL oxidadas (LDLox), que possuem propriedades quimiotáticas e imunogênicas, levando à formação de anticorpos (acLDLox). É fundamental o conhecimento das repercussões metabólicas e ateroscleróticas da LDLox e seus anticorpos no plasma de adultos assintomáticos. Os objetivos deste estudo foram determinar a ação aterogênica da LDLox e dos acLDLox e seus determinantes metabólicos e antropométricos. Foram selecionados 107 indivíduos e classificados em tercis de acordo com os níveis de LDLox e acLDLox. As análises bioquímicas foram realizadas por métodos enzimáticos-colorimétricos, nefelométricos e radiométricos (CETP, PLTP, LH e LPL). A LDLox plasmática foi determinada por ELISA (Mercodia) e os acLDLox contra a LDL inteira...

‣ Influences of sex and age on biological rhythms of serum lipids and lipoproteins

DALPINO, Fabio B.; MENNA-BARRETO, Luiz; FARIA, Eliana C. de
Fonte: ELSEVIER SCIENCE BV Publicador: ELSEVIER SCIENCE BV
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.81316%
Background: Few studies have evaluated seasonal variations of biochemical parameters routinely analyzed in clinical laboratories. Rhythmic patterns for lipids and lipoproteins have been demonstrated and have been the object of research, mainly because of their demonstrated association with coronary artery disease. This study evaluated the occurrence of biological rhythms on serum lipids and lipoproteins and the effects of sex and age on the rhythms in a Brazilian hospital outpatient population. Methods: Retrospective laboratory study was carried out to evaluate the results of total cholesterol (TC), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C) and triglycerides (TG), from individuals registered at a university referral hospital over 8years. The studied population was composed of individuals of both sexes and all ages totaling 38,579 participants and 301,934 measurements. Statistical analyses were carried out using the SAS program and the temporal analysis used the Cosinor method. Results: TG rhythm was present only in females. All other parameters were equally rhythmic in both sexes. Regarding age, HDL-C presented rhythms in all age groups, but TC and LDL-C showed seasonality only for those > 13years, TG did not present rhythms in all age groups. Conclusion: Effects of sex and age on biological rhythms detected in TC...

‣ Reversible flow of cholesteryl ester between high-density lipoproteins and triacylglycerol-rich particles is modulated by the fatty acid composition and concentration of triacylglycerols

CAZITA, P.M.; CASTILHO, L.N.; CARVALHO, M.D.T.; SESSO, A.C.; OLIVEIRA, H.C.F.; QUINTÃO, E.C.R.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
36.900894%
We determined the influence of fasting (FAST) and feeding (FED) on cholesteryl ester (CE) flow between high-density lipoproteins (HDL) and plasma apoB-lipoprotein and triacylglycerol (TG)-rich emulsions (EM) prepared with TG-fatty acids (FAs). TG-FAs of varying chain lengths and degrees of unsaturation were tested in the presence of a plasma fraction at d > 1.21 g/mL as the source of CE transfer protein. The transfer of CE from HDL to FED was greater than to FAST TG-rich acceptor lipoproteins, 18% and 14%, respectively. However, percent CE transfer from HDL to apoB-containing lipoproteins was similar for FED and FAST HDL. The CE transfer from HDL to EM depended on the EM TG-FA chain length. Furthermore, the chain length of the monounsaturated TG-containing EM showed a significant positive correlation of the CE transfer from HDL to EM (r = 0.81, P < 0.0001) and a negative correlation from EM to HDL (r = -041, P = 0.0088). Regarding the degree of EM TG-FAs unsaturation, among EMs containing C18, the CE transfer was lower from HDL to C18:2 compared to C18:1 and C18:3, 17.7%, 20.7%, and 20%, respectively. However, the CE transfer from EMs to HDL was higher to C18:2 than to C18:1 and C18:3, 83.7%, 51.2%, and 46.3%, respectively. Thus...

‣ Are apolipoproteins A and B better than lipoproteins for assessing risk of obstructive coronary heart disease?

Manfroi,Waldomiro Carlos; Zago,Alcides José; Campos,Marcelo; Alves,Alexandre; Brisolara,Maria Lúcia; Souza,Josiane de; Candiago,Rafael Henriques; Kirschnick,Luciana; Ribeiro,Letícia; Ordovás,Kárem; Leitão,Cristiane; Cruz,Rosana
Fonte: Sociedade Brasileira de Cardiologia - SBC Publicador: Sociedade Brasileira de Cardiologia - SBC
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/1999 Português
Relevância na Pesquisa
36.900894%
OBJECTIVE: To evaluate whether apolipoproteins A-I (Apo A-I) and B (Apo B) have, higher ensitivity (SN), specificity (SP) and positive predictive value (PPV) than lipoproteins (LP), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL), and triglycerides (TGL) in assessing the risk of coronary heart disease (CHD). METHODS: This is a transversal case-control study of 241 patients, who were divided into two groups: 1) 145 patients with CHD, and 2) 96 patients without coronary disease. A model of logistic regression to evaluate the relation between the LPs and CHD was developed in which variables with a p-alpha <0.1 were included. RESULTS: Apo A-I levels were higher in the patients without CHD, (OR 2.08, CI 1.20-3.57). There were no statistical differences between the values of Apo A-I and the remaining lipid fractions (Apo A-I: 67%; Apo B: 100%; PPV: TC= 71%; TGC=71%; HDL=71%; LDL=71%). The costs of the tests in Reais were as follows: Apo A-I: R$ 56.60; Apo B-100: R$ 56.60; TC: R$ 9.94; HDL: R$ 21.30; LDL: R$ 28.40; TGL: R$ 14.20. CONCLUSION: Levels of Apo A-I and Apo B have no advantage over conventional lipoproteins in predicting the risk of CHD, despite the statistical association between Apo A-I and CHD; in addition...

‣ Lipolysis of emulsion models of triglyceride-rich lipoproteins is altered in male patients with abdominal aorta aneurysm

Hosni,J.J.; Vinagre,C.G.; Mady,C.; Maranhão,R.C.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2007 Português
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36.683647%
Disorders of the lipid metabolism may play a role in the genesis of abdominal aorta aneurysm. The present study examined the intravascular catabolism of chylomicrons, the lipoproteins that carry the dietary lipids absorbed by the intestine in the circulation in patients with abdominal aorta aneurysm. Thirteen male patients (72 ± 5 years) with abdominal aorta aneurysm with normal plasma lipid profile and 13 healthy male control subjects (73 ± 5 years) participated in the study. The method of chylomicron-like emulsions was used to evaluate this metabolism. The emulsion labeled with 14C-cholesteryl oleate and ³H-triolein was injected intravenously in both groups. Blood samples were taken at regular intervals over 60 min to determine the decay curves. The fractional clearance rate (FCR) of the radioactive labels was calculated by compartmental analysis. The FCR of the emulsion with ³H-triolein was smaller in the aortic aneurysm patients than in controls (0.025 ± 0.017 vs 0.039 ± 0.019 min-1; P < 0.05), but the FCR of14C-cholesteryl oleate of both groups did not differ. In conclusion, as indicated by the triglyceride FCR, chylomicron lipolysis is diminished in male patients with aortic aneurysm, whereas the remnant removal which is traced by the cholesteryl oleate FCR is not altered. The results suggest that defects in the chylomicron metabolism may represent a risk factor for development of abdominal aortic aneurysm.

‣ Reversible flow of cholesteryl ester between high-density lipoproteins and triacylglycerol-rich particles is modulated by the fatty acid composition and concentration of triacylglycerols

Cazita,P.M.; Castilho,L.N.; Carvalho,M.D.T.; Sesso,A.C.; Oliveira,H.C.F.; Quintão,E.C.R.
Fonte: Associação Brasileira de Divulgação Científica Publicador: Associação Brasileira de Divulgação Científica
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2010 Português
Relevância na Pesquisa
36.900894%
We determined the influence of fasting (FAST) and feeding (FED) on cholesteryl ester (CE) flow between high-density lipoproteins (HDL) and plasma apoB-lipoprotein and triacylglycerol (TG)-rich emulsions (EM) prepared with TG-fatty acids (FAs). TG-FAs of varying chain lengths and degrees of unsaturation were tested in the presence of a plasma fraction at d > 1.21 g/mL as the source of CE transfer protein. The transfer of CE from HDL to FED was greater than to FAST TG-rich acceptor lipoproteins, 18% and 14%, respectively. However, percent CE transfer from HDL to apoB-containing lipoproteins was similar for FED and FAST HDL. The CE transfer from HDL to EM depended on the EM TG-FA chain length. Furthermore, the chain length of the monounsaturated TG-containing EM showed a significant positive correlation of the CE transfer from HDL to EM (r = 0.81, P < 0.0001) and a negative correlation from EM to HDL (r = -041, P = 0.0088). Regarding the degree of EM TG-FAs unsaturation, among EMs containing C18, the CE transfer was lower from HDL to C18:2 compared to C18:1 and C18:3, 17.7%, 20.7%, and 20%, respectively. However, the CE transfer from EMs to HDL was higher to C18:2 than to C18:1 and C18:3, 83.7%, 51.2%, and 46.3%, respectively. Thus...

‣ Oxidative stress and plasma lipoproteins in cancer patients

Maia,Fernanda Maria Machado; Santos,Emanuelly Barbosa; Reis,Germana Elias
Fonte: Instituto Israelita de Ensino e Pesquisa Albert Einstein Publicador: Instituto Israelita de Ensino e Pesquisa Albert Einstein
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2014 Português
Relevância na Pesquisa
36.683647%
Objective To evaluate the relation between oxidative stress and lipid profile in patients with different types of cancer. Methods This was an observational cross-sectional. A total of 58 subjects were evaluated, 33 males, divided into two groups of 29 patients each: Group 1, patients with cancer of the digestive tract and accessory organs; Group 2 patients with other types of cancers, all admitted to a public hospital. The plasma levels (lipoproteins and total cholesterol, HDL, and triglycerides, for example) were analyzed by enzymatic kits, and oxidative stress based on thiobarbituric acid-reactive substances, by assessing the formation of malondialdehyde. Results In general the levels of malondialdehyde of patients were high (5.00μM) as compared to 3.31μM for healthy individuals. The median values of lipids exhibited normal triacylglycerol (138.78±89.88mg/dL), desirable total cholesterol values (163.04±172.38mg/dL), borderline high LDL (151.30±178.25mg/dL) and low HDL (31.70±22.74mg/dL). Median HDL levels in Group 1 were lower (31.32mg/dL) than the cancer patients in Group 2 (43.67mg/dL) (p=0.038). Group 1 also showed higher levels of oxidative stress (p=0.027). Conclusion The lipid profile of patients with cancer was not favorable...

‣ Protection of Neurons from Apoptosis by Apolipoprotein E-containing Lipoproteins Does Not Require Lipoprotein Uptake and Involves Activation of Phospholipase Cγ1 and Inhibition of Calcineurin*

Hayashi, Hideki; Campenot, Robert B.; Vance, Dennis E.; Vance, Jean E.
Fonte: American Society for Biochemistry and Molecular Biology Publicador: American Society for Biochemistry and Molecular Biology
Tipo: Artigo de Revista Científica
Português
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Apolipoprotein E-containing lipoproteins (LpE) are generated in the central nervous system by glial cells, primarily astrocytes, and are recognized as key players in lipid metabolism and transport in the brain. We previously reported that LpE protect retinal ganglion neurons from apoptosis induced by withdrawal of trophic additives (Hayashi, H., Campenot, R. B., Vance, D. E., and Vance, J. E. (2007) J. Neurosci. 27, 1933–1941). LpE bind to low density lipoprotein receptor-related protein-1 and initiate a signaling pathway that involves activation of protein kinase Cδ and inhibition of the pro-apoptotic glycogen synthase kinase-3β. We now show that uptake of LpE is not required for the neuroprotection. Experiments with inhibitors of phospholipase Cγ1 and RNAi knockdown studies demonstrate that activation of phospholipase Cγ1 is required for the anti-apoptotic signaling pathway induced by LpE. In addition, the protein phosphatase-2B, calcineurin, is involved in a neuronal death pathway induced by removal of trophic additives, and LpE inhibit calcineurin activation. LpE also attenuate neuronal death caused by oxidative stress. Moreover, physiologically relevant apoE3-containing lipoproteins generated by apoE3 knock-in mouse astrocytes more effectively protect neurons from apoptosis than do apoE4-containing lipoproteins. Because inheritance of the apoE4 allele is the strongest known genetic risk factor for Alzheimer disease...

‣ Racial differences between African-American and white women in insulin resistance and visceral adiposity are associated with differences in apoCIII containing apoAI and apoB lipoproteins

Wang, Liyun; Sacks, Frank M; Furtado, Jeremy D; Ricks, Madia; Courville, Amber B; Sumner, Anne E
Fonte: BioMed Central Publicador: BioMed Central
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
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Background: African-Americans have higher HDL, less visceral adipose tissue (VAT) and lower triglyceride (TG) and apoCIII concentrations than whites, despite being more insulin-resistant. We studied in African-American and white women the influences of insulin resistance and VAT on the apoAI concentrations of two HDL subspecies, one that contains apoCIII that is associated with increased risk of coronary heart disease (CHD) and one that does not have apoCIII that is associated with decreased CHD; and on the apoCIII concentrations of HDL and of the apoB lipoproteins. Methods: The participants were 32 women (14 African-Americans, 18 white) of similar age (39 ± 12 vs. 42 ± 11y). Mean BMI was 34 kg/m2 in the African-Americans compared to 30 in the whites. A standard diet (33% fat, 52% carbohydrate, 15% protein) was provided for 7 days followed by a test meal (40% fat, 40% carbohydrate, 20% protein) on Day 8. Insulin sensitivity index (SI) was calculated from the minimal model. Results: After controlling for SI, African-Americans have a higher mean apoAI level in HDL with apoCIII compared with whites (12.9 ± 2.8 and 10.9 ± 2.9 mg/dL, respectively, P = 0.05). SI was associated with higher apoAI in HDL with apoCIII, whereas VAT was not associated with this HDL subspecies. This pattern of results was reversed for apoCIII concentrations in apoB lipoproteins. After adjusting for SI...

‣ Pesquisa de anticorpos IgG séricos anti-lipoproteínas de mycoplasma fermentans e mycoplasma hominis ou anti-mam (superantigeno de mycoplasma arthritidis) em pacientes com artrite reumatoide ou lupus eritematoso sistemico; Search IgG anti-serum lipoproteins mycoplasma fermentans and mycoplasma hominis or anti-mam (superantigen mycoplasma arthritidis) in patients with rheumatoid arthritis or lupus erythematosus systemic

Rocha Sobrinho, Hermínio Maurício da
Fonte: Universidade Federal de Goiás; Brasil; UFG; Programa 1; Faculdade 1::Departamento 1 Publicador: Universidade Federal de Goiás; Brasil; UFG; Programa 1; Faculdade 1::Departamento 1
Tipo: Dissertação Formato: application/pdf
Português
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Rheumatoid Arthritis (RA) and Systemic Lupus Erythematosus (SLE) are autoimmune diseases of unknown etiology. Some species of mycoplasmas cause arthritis in animals and humans, and their lipid-associated membrane proteins (LAMPs) and Mycoplasma arthritidis mitogen (MAM superantigen) are potent stimulators of the immune system. Thus, it has been proposed that mycoplasma can be involved in autoimmune-disease etiology. The objective of the present work was to detect antibodies to MAM and LAMPs of M. hominis and M. fermentans in the patient sera, and to characterize the profile of IgG antibodies reactivity with LAMPs in order to identify the major immunogenic mycoplasmal lipoproteins that could be involved in the etiopathogenesis of these autoimmune diseases. Serum samples were obtained from peripheral blood of female patients at the same age of healthy controls. Recombinant MAM (from M. arthrititidis), LAMPs of M. hominis PG21 and M. fermentans PG18 were used in Western blotting assays. Antibodies to MAM were detected in the patient and control sera (RA: 27.5% vs 18.8%; SLE: 21.7% vs 20.0%). At least 23 LAMPs were found in the preparations of M. hominis PG21 and of M. fermentans PG18 with molecular masses between 20 and 192 KDa. The sera of RA patients recognized a larger number of LAMPs of M. hominis PG21 and M. fermentans PG18 than the control sera (RA: 11 ± 4 vs controls: 7 ± 3...

‣ Native and oxidized low density lipoproteins enhance platelet aggregation in whole blood

Tornvall, P.; Chirkova, L.; Toverud, K.; Horowitz, J.; Chirkov, Y.; Gustaf, K.
Fonte: Elsevier Publicador: Elsevier
Tipo: Artigo de Revista Científica
Publicado em //1999 Português
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The effects of native and oxidized low density lipoproteins on platelet aggregability remain controversial despite numerous studies. In the current investigation, effects of native, minimally and extensively copper-modified low-density lipoproteins on aggregation responses to ADP and collagen in platelet-rich plasma, washed platelets, and whole blood were studied. Preincubation with native and oxidized low-density lipoproteins (1.5-23.2 malondialdehyde equivalents [MDAeq]/mg low density lipoprotein) did not modify aggregability in platelet-rich plasma or washed platelets but increased aggregation markedly in whole blood (40-58%, p<0.01). In whole blood, the increase in response to ADP was not affected by the degree of low-density lipoprotein oxidation. This comprehensive investigation of low density lipoprotein effects on platelet aggregation therefore has demonstrated that low density lipoproteins indirectly increase platelet aggregability in whole blood, but not in platelet-rich plasma and washed platelets, presumably via an interaction between platelets and other formed elements of blood.

‣ Rol de las lipoproteínas de Brucella Abortus en el desarrollo de la respuesta inflamatoria en brucelosis; Role of Brucella abortus lipoproteins in the development of the inflammatory response in brucellosis

Zwerdling, Astrid
Fonte: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires Publicador: Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires
Tipo: info:eu-repo/semantics/doctoralThesis; tesis doctoral; info:eu-repo/semantics/publishedVersion Formato: application/pdf
Publicado em //2009 Português
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La inflamación es un rasgo característico de la brucelosis humana, presente virtualmente en todos los órganos afectados por la misma. Esta particularidad, junto con la detección de bacterias en los tejidos inflamados, sugiere que Brucella estimula una robusta respuesta inflamatoria en los sitios en que se localiza. Sin embargo, los mecanismos por los cuales estas bacterias desencadenan esta respuesta son hasta el momento desconocidos. A pesar del poderoso potencial inmunomodulador de las lipoproteínas bacterianas, se les ha prestado hasta el momento poca atención en la brucelosis. En esta tesis investigamos la función de las lipoproteínas en el desarrollo de la respuesta inflamatoria asociada a esta enfermedad, poniendo énfasis en la interacción de Brucella con distintos tipos celulares fundamentales en el desarrollo de la respuesta innata. Nuestros resultados demuestran que B. abortus es capaz de inducir la respuesta inflamatoria por medio de la activación y producción de citoquinas en monocitos/macrófagos, células dendríticas y neutrófilos. En relación al componente de Brucella responsable de inducir esta respuesta, nuestras observaciones experimentales indican que las lipoproteínas de Brucella estarían implicadas en el desarrollo de la respuesta inflamatoria en brucelosis.; Regardless of the diversity of signs and symptoms of human brucellosis...

‣ Recent advances in lipoproteins and atherosclerosis: A nutrigenomic approach; Nuevos avances sobre el efecto de las lipoproteínas en la aterosclerosis: Una aproximación nutrigenómica

López, Sergio; Ortega, Almudena; Varela, Lourdes; Bermúdez, Beatriz; Muriana, Francisco J. G.; Abia, Rocío
Fonte: Consejo Superior de Investigaciones Científicas (España) Publicador: Consejo Superior de Investigaciones Científicas (España)
Tipo: Artículo Formato: 63313 bytes; application/pdf
Português
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8 pages.-- Review article.; [EN] Atherosclerosis is a disease in which multiple factors contribute to the degeneration of the vascular wall. Many risk factors have been identified as having influence on the progression of atherosclerosis among them, the type of diet. Multifactorial interaction among lipoproteins, vascular wall cells, and inflammatory mediators has been recognised as the basis of atherogenesis. Dietary intake affects lipoprotein concentration and composition providing risk or protection at several stages of atherosclerosis. More intriguingly, it has been demonstrated that the extent to which each lipid or lipoprotein is associated with cardiovascular disease depends on the time to last meal; thus, postprandial lipoproteins, main lipoproteins in blood after a high-fat meal, have been shown to strongly influence atherogenesis. As a complex biological process, the full cellular and molecular characterization of atherosclerosis derived by diet, calls for application of the newly developing “omics” techniques of analysis. This review will considered recent studies using high-throughput technologies and a nutrigenomic approach to reveal the patho-physiological effects that the fasting and postprandial lipoproteins may exert on the vascular wall.; [ES] La aterosclerosis es una enfermedad en la que múltiples factores...