Página 1 dos resultados de 145 itens digitais encontrados em 0.001 segundos

‣ A novel whey protein synthesized only in late lactation by the mammary gland from the tammar (Macropus eugenii).

Nicholas, K R; Messer, M; Elliott, C; Maher, F; Shaw, D C
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 01/02/1987 Português
Relevância na Pesquisa
49.117856%
A major whey protein which appears in milk from the tammar wallaby (Macropus eugenii) only during the second half of lactation (late lactation protein-A, LLP-A) was purified to apparent homogeneity by ion-exchange chromatography and gel filtration. An Mr of 21,600 +/- 2000 was calculated from its amino acid composition. A computer-based comparison of the sequence of the first 69 amino acid residues with the Atlas of Protein Sequence data base showed no significant homology with known proteins. Antiserum to LLP-A was prepared in rabbits, and single radial immunodiffusion was used to measure the amounts of LLP-A in milk during the first 40 weeks of lactation. LLP-A was first detected at 26 weeks; thereafter its concentration increased abruptly, to reach a maximum of 26 g/l at approx. 36 weeks of lactation. Explants prepared from mammary gland biopsies at 20 and 35 weeks of lactation were exposed to [3H]amino acids for 8 h; immunoprecipitation of tissue extracts showed that, whereas the rate of casein synthesis was the same at both stages of lactation, LLP-A was synthesized only by the 35-week mammary gland.

‣ The tammar wallaby (Macropus eugenii) and the Sprague-Dawley rat: comparative anatomy and physiology of inguinoscrotal testicular descent.

Griffiths, A L; Renfree, M B; Shaw, G; Watts, L M; Hutson, J M
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /12/1993 Português
Relevância na Pesquisa
49.117856%
Inguinoscrotal testicular descent in the tammar wallaby (Macropus eugenii) and the Sprague-Dawley rat was studied by macroscopic dissection, histological evaluation and organ culture bioassay. In 3 or 4 d Sprague-Dawley rats (n = 10) the gubernacular tip bulged free from the surrounding tissues, particularly with the application of abdominal pressure. Microscopic examination revealed that only the body of the gubernaculum is connected posteriorly to the pubic region. In contrast, macroscopic dissection of male tammar wallabies (n = 17) revealed a densely adherent distal gubernacular attachment to the inside of the fibrous scrotal bulge while the body of the gubernaculum was less firmly attached. These attachments were present throughout the process of testicular descent, illustrating an important anatomical difference between these species. The gubernaculum from the tammar wallaby pouch young was studied in organ culture with rat calcitonin gene-related peptide for 4 d. Rhythmic gubernacular contractions similar to those documented previously in the rat were not observed. The hypothesis proposed in the rat for the control of inguinoscrotal gubernacular migration via the genitofemoral nerve and its neurotransmitters may not be applicable in marsupial mammals.

‣ The first comprehensive genetic linkage map of a marsupial: the tammar wallaby (Macropus eugenii).

Zenger, Kyall R; McKenzie, Louise M; Cooper, Desmond W
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /09/2002 Português
Relevância na Pesquisa
49.117856%
The production of a marsupial genetic linkage map is perhaps one of the most important objectives in marsupial research. This study used a total of 353 informative meioses and 64 genetic markers to construct a framework genetic linkage map for the tammar wallaby (Macropus eugenii). Nearly all markers (93.8%) formed a significant linkage (LOD > 3.0) with at least one other marker, indicating that the majority of the genome had been mapped. In fact, when compared with chiasmata data, >70% (828 cM) of the genome has been covered. Nine linkage groups were identified, with all but one (LG7; X-linked) allocated to the autosomes. These groups ranged in size from 15.7 to 176.5 cM and have an average distance of 16.2 cM between adjacent markers. Of the autosomal linkage groups (LGs), LG2 and LG3 were assigned to chromosome 1 and LG4 localized to chromosome 3 on the basis of physical localization of genes. Significant sex-specific distortions toward reduced female recombination rates were revealed in 22% of comparisons. When comparing the X chromosome data to closely related species it is apparent that they are conserved in both synteny and gene order.

‣ Spermiogenesis and spermiation in a marsupial, the tammar wallaby (Macropus eugenii)

LIN, MINJIE; HARMAN, AMANDA; RODGER, JOHN C.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /04/1997 Português
Relevância na Pesquisa
49.117856%
Fourteen steps of spermatid development in the tammar wallaby (Macropus eugenii), from the newly formed spermatid to the release of the spermatozoon into the lumen of the seminiferous tubules, were recognised at the ultrastructural level using transmission and scanning electron microscopy. This study confirmed that although the main events are generally similar, the process of the differentiation of the spermatid in marsupials is notably different and relatively more complex than that in most studied eutherian mammals and birds. For example, the sperm head rotated twice in the late stage of spermiogenesis: the shape of the spermatid changed from a T-shape at step 10 into a streamlined shape in step 14, and then back to T-shape in the testicular spermatozoa. Some unique figures occurring during the spermiogenesis in other marsupial species, such as the presence of Sertoli cell spurs, the nuclear ring and the subacrosomal space, were also found in the tammar wallaby. However, an important new finding of this study was the development of the postacrosome complex (PAC), a special structure that was first evident as a line of electron dense material on the nuclear membrane of the step 7 spermatid. Subsequently it became a discontinuous line of electron particles...

‣ Acrosome formation during sperm transit through the epididymis in two marsupials, the tammar wallaby (Macropus eugenii) and the brushtail possum (Trichosurus vulpecula)

LIN, MINJIE; RODGER, JOHN C.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /02/1999 Português
Relevância na Pesquisa
49.117856%
In certain Australian marsupials including the tammar wallaby (Macropus eugenii) and the brushtail possum (Trichosurus vulpecula), formation of the acrosome is not completed in the testis but during a complex differentiation process as spermatozoa pass through the epididymis. Using transmission and scanning electron microscopy this paper defined the process of acrosome formation in the epididymis, providing temporal and spatial information on the striking reorganisation of the acrosomal membranes and matrix and of the overlying sperm surface involved. On leaving the testis wallaby and possum spermatozoa had elongated ‘scoop’-shaped acrosomes projecting from the dorsal surface of the head. During passage down the epididymis, this structure condensed into the compact button-like organelle found on ejaculated spermatozoa. This condensation was achieved by a complex process of infolding and fusion of the lateral projections of the ‘scoop’. In the head of the epididymis the rims of the lateral scoop projections became shorter and thickened and folded inwards, to eventually meet midway along the longitudinal axis of the acrosome. As spermatozoa passed through the body of the epididymis the lateral projections fused together. Evidence of this fusion of the immature outer acrosomal membrane is the presence of vesicles within the acrosomal matrix which persist even in ejaculated spermatozoa. When spermatozoa have reached the tail of the epididymis the acrosome condenses into its mature form...

‣ Actin polymerisation during morphogenesis of the acrosome as spermatozoa undergo epididymal maturation in the tammar wallaby (Macropus eugenii)

SCARLETT, CHRIS J. ; LIN, MINJIE; AITKEN, R. JOHN
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /01/2001 Português
Relevância na Pesquisa
49.117856%
In the tammar wallaby (Macropus eugenii), post-testicular acrosomal shaping involves a complex infolding and fusion of the anterior and lateral projections of the scoop-shaped acrosome into a compact button-like structure occupying the depression on the anterior end of the sperm nucleus. The present study has generated cytochemical and histological evidence to demonstrate that the occurrence of actin filaments (F-actin, labelled by Phalloidin-FITC) in the acrosome of tammar wallaby spermatozoa is temporally and spatially associated with the process of acrosomal shaping in the epididymis, through a pool of monomeric actin (G-actin, labelled by Rh-DNase I) present in the acrosome throughout all stages of epididymal maturation. F-actin was not detected in the acrosome of testicular spermatozoa, but was found in the infolding and condensing acrosome of caput and corpus epididymal spermatozoa. When the spermatozoa completed acrosome shaping in the cauda epididymidis, F-actin disappeared from the acrosomal area. The strong correlation between the occurrence of F-actin and the events of acrosomal shaping suggested that the post-testicular shaping of the acrosome might depend on a precise succession of assembly and disassembly of F-actin within the acrosome as the spermatozoa transit the epididymis. Thus...

‣ The development of the gubernaculum and inguinal closure in the marsupial Macropus eugenii

Coveney, Douglas; Shaw, Geoffrey; Hutson, John M; Renfree, Marilyn B
Fonte: Blackwell Science Inc Publicador: Blackwell Science Inc
Tipo: Artigo de Revista Científica
Publicado em /09/2002 Português
Relevância na Pesquisa
49.117856%
This study reports the developmental anatomy of testicular descent and inguinal closure of the tammar wallaby (Macropus eugenii) from birth to maturity. In females the ovary migrated caudally between days 10 and 20 after birth. The gubernaculum differentiates into the round ligament in the abdomen and extra-abdominally as the ilio-marsupialis muscle of the mammary glands. In males the testes migrated to the internal inguinal ring by day 20 post partum (pp), coinciding with the enlargement of the gubernaculum, and from the internal inguinal ring to the scrotum between days 20 and 65 pp. During descent there was an increase in the hyaluronic acid concentration in cells of the gubernaculum and scrotum. Development of the cremaster muscle began by day 10 pp on the periphery of the gubernaculum and its basic structure was completed by day 60 pp. After descent the inguinal canal closed between days 50 and 60 pp, but a small irregular lumen persisted, somewhat similar to that seen in the congenital scrotal hydrocoele of humans. Tammars have a hopping mode of locomotion and, like humans, are essentially bipedal. We suggest that inguinal closure evolved in these two species because their upright posture may otherwise lead to a high incidence of inguinal hernias.

‣ Immunohistochemistry of the lymphoid tissues of the tammar wallaby, Macropus eugenii

Old, Julie M; Deane, Elizabeth M
Fonte: Blackwell Science Inc Publicador: Blackwell Science Inc
Tipo: Artigo de Revista Científica
Publicado em /09/2002 Português
Relevância na Pesquisa
49.117856%
The lymphoid tissues of the metatherian mammal, the adult tammar wallaby, Macropus eugenii, were investigated using immunohistochemical techniques. Five cross-reactive antibodies previously shown to recognize surface markers in marsupial tissues and five previously untested antibodies were used. The distribution of T-cells in the tissue beds of spleen, lymph node, thymus, gut-associated lymphoid tissue (GALT) and bronchus-associated lymphoid tissue (BALT) was documented using antibodies to CD3 and CD5. Similarly, B-cells were identified in the same tissues using anti-CD79b. Antibodies to CD8, CD31, CD79a and CD68 failed to recognize cells in these tissue beds. In general the pattern of cellular distribution identified using these antibodies was similar to that observed in other marsupial and eutherian lymphoid tissues. This study provides further information on the commonality of lymphoid tissue structure in the two major groups of extant mammals, metatherians and eutherians.

‣ Community Composition and Density of Methanogens in the Foregut of the Tammar Wallaby (Macropus eugenii)▿

Evans, Paul N.; Hinds, Lyn A.; Sly, Lindsay I.; McSweeney, Christopher S.; Morrison, Mark; Wright, André-Denis G.
Fonte: American Society for Microbiology (ASM) Publicador: American Society for Microbiology (ASM)
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
49.117856%
The composition of the methanogenic archaeal community in the foregut contents of Tammar wallabies (Macropus eugenii) was studied using 16S rRNA and methyl coenzyme reductase subunit A (mcrA) gene clone libraries. Methanogens belonging to the Methanobacteriales and a well-supported cluster of uncultivated archaeon sequences previously observed in the ovine and bovine rumens were found. Methanogen densities ranged from 7.0 × 105 and 3.9 × 106 cells per gram of wet weight.

‣ Dynamics of leg muscle function in tammar wallabies (M-eugenii) during level versus incline hopping

Biewener, Andrew A.; McGowan, Craig P.; Card, G. M.; Baudinette, Russell Victor
Fonte: Company of Biologists Publicador: Company of Biologists
Tipo: Artigo de Revista Científica
Publicado em //2004 Português
Relevância na Pesquisa
59.41236%
The goal of our study was to examine whether the in vivo force-length behavior, work and elastic energy savings of distal muscle-tendon units in the legs of tammar wallabies (Macropus eugenii) change during level versus incline hopping. To address this question, we obtained measurements of muscle activation (via electromyography), fascicle strain (via sonomicrometry) and muscle-tendon force (via tendon buckles) from the lateral gastrocnemius (LG) and plantaris (PL) muscles of tammar wallabies trained to hop on a level and an inclined (10°, 17.4% grade) treadmill at two speeds (3.3 m s-1 and 4.2 m s-1). Similar patterns of muscle activation, force and fascicle strain were observed under both level and incline conditions. This also corresponded to similar patterns of limb timing and movement (duty factor, limb contact time and hopping frequency). During both level and incline hopping, the LG and PL exhibited patterns of fascicle stretch and shortening that yielded low levels of net fascicle strain [LG: level, -1.0±4.6% (mean ± S.E.M.) vs incline, 0.6±4.5%; PL: level, 0.1±1.0% vs incline, 0.4±1.6%] and muscle work (LG: level, -8.4±8.4 J kg-1 muscle vs incline, -6.8±7.5 J kg-1 muscle; PL: level, -2.0±0.6 J kg-1 muscle vs incline...

‣ Postnatal development and control of the pulmonary surfactant system in the tammar wallaby Macropus eugenii

Miller, Natalie J.; Orgeig, Sandra; Daniels, Christopher Brian; Baudinette, Russell Victor
Fonte: The Company of Biologists Ltd Publicador: The Company of Biologists Ltd
Tipo: Artigo de Revista Científica
Publicado em //2001 Português
Relevância na Pesquisa
69.706123%
Marsupials are born at an early stage of development and are adapted for future development inside the pouch. Whether the pulmonary surfactant system is fully established at this altricial stage is unknown. This study correlates the presence of surfactant proteins (SP-A, SP-B and SP-D), using immunohistochemistry, with the ex-utero development of the lung in the tammar wallaby Macropus eugenii and also investigates the control of phosphatidylcholine (PC) secretion from developing alveolar type II cells. All three surfactant proteins were found at the site of gas exchange in the lungs of joeys at all ages, even at birth when the lungs are in the early stages of the terminal air-sac phase. Co-cultures of alveolar type II cells and fibroblasts were isolated from the lungs of 30- and 70-day-old joeys and incubated with the hormones dexamethasone (10 µmol l–1), prolactin (1 µmol l–1) or triiodothyronine (100 µmol l–1) or with the autonomic secretagogues isoproterenol (100 µmol l–1) or carbamylcholine chloride (100 µmol l–1). Basal secretion of PC was greater at 30 days of age than at 70 days. Co-cultures responded to all five agonists at 30 days of age, but only the autonomic secretagogues caused a significant increase in PC secretion at 70 days of age. This demonstrates that...

‣ Characterizing the chromosomes of the Australian model marsupial Macropus eugenii (tammar wallaby)

Alsop, A.; Miethke, P.; Rofe, R.; Koina, E.; Sankovic, N.; Deakin, J.; Haines, H.; Rapkins, R.; Marshall Graves, J.
Fonte: Kluwer Academic Publ Publicador: Kluwer Academic Publ
Tipo: Artigo de Revista Científica
Publicado em //2005 Português
Relevância na Pesquisa
59.41162%
Marsupials occupy a phylogenetic middle ground that is very valuable in genome comparisons of mammal and other vertebrate species. For this reason, whole genome sequencing is being undertaken for two distantly related marsupial species, including the model kangaroo species Macropus eugenii (the tammar wallaby). As a first step towards the molecular characterization of the tammar genome, we present a detailed description of the tammar karyotype, report the development of a set of molecular anchor markers and summarize the comparative mapping data for this species.; Amber E. Alsop, Pat Miethke, Ruth Rofe, Edda Koina, Natasha Sankovic, Janine E. Deakin, Helen Haines, Robert W. Rapkins and Jennifer A. Marshall Graves

‣ The α-globin gene family of an Australian marsupial, Macropus eugenii: The long evolutionary history of the θ-globin gene and its functional status in mammals; The alpha-globin gene family of an Australian marsupial, Macropus eugenii: The long evolutionary history of the theta-globin gene and its functional status in mammals

Cooper, S.; Wheeler, D.; Hope, R.; Dolman, G.; Saint, K.; Gooley, A.; Holland, R.
Fonte: Springer Publicador: Springer
Tipo: Artigo de Revista Científica
Publicado em //2005 Português
Relevância na Pesquisa
49.706123%
Comparative evolutionary analyses of gene families among divergent lineages can provide information on the order and timing of major gene duplication events and evolution of gene function. Here we investigate the evolutionary history of the α-globin gene family in mammals by isolating and characterizing α-like globin genes from an Australian marsupial, the tammar wallaby, Macropus eugenii. Sequence and phylogenetic analyses indicate that the tammar α -globin family consists of at least four genes including a single adult-expressed gene (α), two embryonic/neonatally expressed genes (ζ and ζ′), and θ -globin, each orthologous to the respective α-, ζ-, and θ-globin genes of eutherian mammals. The results suggest that the θ -globin lineage arose by duplication of an ancestral adult α-globin gene and had already evolved an unusual promoter region, atypical of all known α-globin gene promoters, prior to the divergence of the marsupial and eutherian lineages. Evolutionary analyses, using a maximum likelihood approach, indicate that θ -globin, has evolved under strong selective constraints in both marsupials and the lineage leading to human θ -globin, suggesting a long-term functional status. Overall, our results indicate that at least a four-gene cluster consisting of three α-like and one β-like globin genes linked in the order 5′–ζ–α–θ–ω–3′ existed in the common ancestor of marsupials and eutherians. However...

‣ Sex ratio theory applied to a macropod marsupial : Is reproduction by the Tammar Wallaby (Macropus eugenii) consistent with sex ratio theory?

Perryman, Aryn White
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2012 Português
Relevância na Pesquisa
69.706123%
Marsupial species provide an important research tool in attempts to unravel the complicated mechanisms of mammalian reproduction. Comparisons between metatherian and eutherian reproductive life history patterns used to achieve the same outcomes, illuminates processes that have been difficult to investigate in eutherian species alone. Adaptive sex allocation is one such area that has provided equivocal data that is much scrutinized and debated amongst scientists. The theory that has provided the most disparity in the literature, and lead to the most debate, is the Trivers-Willard Model (TWM) (Trivers & Willard, 1973). The TWM proposes that, in polygynous mammalian species where the male has increased variability in reproductive returns, mothers in ‘good condition’ should bias their offspring towards sons, to maximise their fitness. There are three main assumptions associated with the theory: 1) that sons will benefit more than daughters from increased maternal resource allocation, 2) that juvenile condition predicts adult condition and 3) that maternal quality is a predictor of offspring quality. Much of the previous support for the TWM has been based on research in eutherian species (e.g. ungulate species), which usually tests only one assumption at a time by a posteriori application of the theory to observed data. Macropod species fill similar niches to ungulate species in other ecosystems and provide easy access to pouch young at a very early stage of development...

‣ Establishment, behaviour and ecology of the SA mainland tammar wallaby (Macropus eugenii eugenii) following an experimental reintroduction.

Kemp, Leah F.
Fonte: Universidade de Adelaide Publicador: Universidade de Adelaide
Tipo: Tese de Doutorado
Publicado em //2011 Português
Relevância na Pesquisa
49.59359%
The South Australian mainland tammar wallaby (Macropus eugenii eugenii) was presumed extinct in the wild from the early 1930's, until a feral population was re-discovered in New Zealand. Eighty-five animals were returned to Australia as part of a repatriation program into their former range. The establishment phase after a reintroduction is a critical time as animals may fail to survive if they cannot find resources and avoid predators in an unfamiliar habitat. To maximise reintroduction and establishment success, reintroductions need to be planned with a good understanding of the animals’ ecology and anti-predator strategies. To improve this understanding requires experimental reintroductions and detailed monitoring. This thesis investigates the experimental reintroduction of 46 wallabies into Innes National Park in South Australia and examines the influence of release group familiarity on establishment. Part of this was an investigation of home range, habitat requirements and social behaviours during establishment and seasonally post-establishment. The thesis includes three data chapters which focus on (1) home range and core area, home range stability and degree of overlap with conspecifics; (2) habitat selection at the landscape scale and for day and night use within home range; and (3) factors influencing fine scale habitat use and social grouping behaviours in light of predation risk. In this study...

‣ Development of the vestibular apparatus and central vestibular connections in a wallaby (Macropus eugenni)

McCluskey, S U; Marotte, Lauren; Ashwell, Ken W S
Fonte: S Karger AG Publicador: S Karger AG
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
59.41162%
We have studied the early development of the vestibular apparatus and its central connections in the tammar wallaby (Macropus eugenii) in order to determine whether the vestibular system anatomy is sufficiently mature at birth to assist in climbing to the

‣ Y chromosome microsatellite markers identified from the tammar wallaby (Macropus eugenii) and their amplification in three other macropod species

Macdonald, Anna J; Sankovic, Natasha; Sarre, Stephen; Fitzsimmons, Nancy; Wakefield, Matthew; Graves, Jennifer; Zengers, Kyall R
Fonte: Blackwell Publishing Ltd Publicador: Blackwell Publishing Ltd
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
69.706123%
Microsatellites were identified from three fully sequenced Y chromosome-specific bacterial artificial chromosome clones from the tammar wallaby, Macropus eugenii. Ten microsatellites were genotyped in male tammar wallabies. Four loci were polymorphic with

‣ Cyto- and Chemoarchitecture of the Cortex of the Tammar Wallaby (Macropus eugenii): Areal Organization

Ashwell, Ken W S; Zhang, L-L; Marotte, Lauren
Fonte: S Karger AG Publicador: S Karger AG
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
69.706123%
We have examined the cyto- and chemoarchitecture of the isocortex of a diprotodontid marsupial, the tammar wallaby (Macropus eugenii), using Nissl staining in combination with enzyme histochemical (acetylcholinesterase - AChE, NADPH-diaphorase - NADPHd, cytochrome oxidase) and immunohistochemical (non-phosphorylated neurofilament - SMI-32) markers. The primary sensory cortex showed distinctive patterns of reactivity in cytochrome oxidase, acetylcholinesterase and NADPH diaphorase. For example, in AChE material, S1 showed a heterogeneous appearance, with regions exhibiting a double layer of AChE activity (layers II and IV) adjacent to poorly reactive regions. In NADPHd preparations, activity in S1 was strongest in layers I to IV although, as in AChE material, there were consistent patches of reduced NADPHd activity which corresponded to poorly reactive regions in the AChE sections. Each of the primary sensory areas of the isocortex showed a different pattern of distribution of SMI-32+ neurons. In V1, SMI-32+ neurons were distributed in two layers (III and V) throughout the tangential extent ofthat region. In S1, SMI-32+ neurons were concentrated in layer V, but large and discrete patches within S1 had additional SMI-32+ neurons in layer III. In primary auditory cortex there was a dense band of SMI-32+ neurons in layer V...

‣ Characterizing the chromosomes of the Australian model marsupial Macropus eugenii (Tammar wallaby)

Alsop, Amber; Miethke, Patricia; Rofe, Ruth; Koina, Edda; Sankovic, Natasha; Deakin, Janine; Haines, Helen; Rapkins, Robert; Graves, Jennifer
Fonte: Kluwer Academic Publishers Publicador: Kluwer Academic Publishers
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
59.41162%
Marsupials occupy a phylogenetic middle ground that is very valuable in genome comparisons of mammal and other vertebrate species. For this reason, whole genome sequencing is being undertaken for two distantly related marsupial species, including the model kangaroo species Macropus eugenii (the tammar wallaby). As a first step towards the molecular characterization of the tammar genome, we present a detailed description of the tammar karyotype, report the development of a set of molecular anchor markers and summarize the comparative mapping data for this species.

‣ Construction of a marsupial bacterial artificial chromosome library from the model Australian marsupial, the tammar wallaby (Macropus eugenii)

Sankovic, Natasha; Bawden, Wayne; Martyn, John; Graves, Jennifer; Zuelke, Kurt
Fonte: CSIRO Publishing Publicador: CSIRO Publishing
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
69.706123%
With the accelerating recognition of the power of comparative genomics, there is now enormous interest in sequencing the genomes of a broad range of species. Marsupials diverged at an important evolutionary time. The model Australian marsupial, the tammar wallaby (Macropus eugenii), has long been a resource for biological and genetic studies of marsupials, and the availability of a bacterial artificial chromosome (BAC) library will be a valuable resource in these studies. A tammar wallaby BAC library was constructed using pRazorBAC vector. It contains 55 296 clones with an average insert size of 108 kb, representing 2.2 times coverage of the wallaby genome (based on an estimated 2.7 × 109 bp haploid genome size). The library was arrayed in 384-well plates, and spotted in duplicate onto nylon membranes. Screening these membranes has yielded clones containing 34 single-copy genes distributed over the genome, while it failed for only one gene. Each probe isolated 1-12 BAC clones and, to date, no chimeric clones have been found. This BAC library will constitute an invaluable resource for creating physical maps, positional cloning of genes and other sequences in the tammar wallaby, as well as comparative mapping studies in mammals.