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‣ Fed State Protein Turnover in Healthy Older Persons under a Usual Protein-Rich Diet

PFRIMER, Karina; MARCHINI, Julio Sergio; MORIGUTI, Julio Cesar; FERRIOLLI, Eduardo
Fonte: WILEY-BLACKWELL PUBLISHING, INC Publicador: WILEY-BLACKWELL PUBLISHING, INC
Tipo: Artigo de Revista Científica
Português
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The objective of this study was to verify the protein turnover rates of healthy older persons under a usual protein-rich diet and to compare values to those described in the literature. This cross-sectional study was conducted at Metabolism Unit, Univ. Hospital of the School of Medicine of Ribeirao Preto, Univ. of Sao Paulo, Brazil. In this study, 7 healthy older persons aged 65.4 +/- 2.8 y, with BMI 22.7 +/- 2.4 kg/m(2) and a mean daily protein intake of 1.34 g of protein/kg were studied. A 9-h whole-body (15)N-glycine single-dose study was performed after an overnight fast. During the study, each subject received 6 isoenergetic, isonitrogenous meals at 2-h intervals based on their average intake. Ammonium, urea, and total nitrogen were quantified and analyzed by mass spectrometry, with the determination of total protein turnover rates by the (15)N-glycine method. The results show that total nitrogen output was 3.2 +/- 0.96 g/N and intake 7.7 +/- 1 g/N, (15)N nitrogen flux was 30.6 +/- 6.3 g/9 h. Endogenous nitrogen balance was positive (4.5g +/- g/N in 9 h). In conclusion, the protein turnover of healthy older persons under a usual protein-rich diet is positive during the fed state and has synthesis and degradation rates similar to those previously described in studies involving diet adaptation periods.; Nacional de Desenvolvimento Cientifico e Tecnologico[477045/2004-9]; IAEA (Intl. Atomic Energy Agency[7008-86572L]; CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel superior...

‣ Avaliação do metabolismo protéico em idosos brasileiros independentes utilizando a glicina marcada com 15N; Protein Metabolism in Brazilian healthy elderly using glycine labeled with 15N

Pfrimer, Karina
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 22/02/2006 Português
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O metabolismo protéico em idosos, analisado pela medida da velocidade de reciclagem, é um importante fator para a análise da manutenção da massa muscular e das atividades de vida diária. Dados coletados em idosos apontam uma redução da síntese protéica com o envelhecimento. Outros relatam ser esta mantida e a degradação aumentada. Esta investigação teve por objetivo avaliar o metabolismo protéico de idosos saudáveis e independentes utilizando a glicina, marcada com o isótopo 15N. Sete idosos saudáveis foram estudados.Foram feitas avaliações clínica, nutricional e bioquímica em todos os voluntários, sendo excluídos aqueles portadores de doenças ou usuários de medicamentos que interferissem no metabolismo protéico. Foi oferecida uma dose oral de 200 mg de 15N-Glicina e coletadas amostras de sangue e urina (basal, antes do consumo da glicina, quatro horas e meia e nove horas após a ingestão da glicina). Foram quantificados amônia, uréia e nitrogênio total e as amostras analisadas por espectrometria de massa, para a determinação do enriquecimento isotópico (15N). Os voluntários tinham 65,4 ± 2,8 anos (média ± desvio padrão), quatro mulheres e três homens, com IMC de 22,73 ± 2,4 Kg/m2. Total de nitrogênio excretado de 3...

‣ Efeito da oferta dietética de proteína sobre o ganho muscular, balanço nitrogenado e cinética da 15N-glicina de atletas em treinamento de musculação

Maesta, Nailza; Cyrino, Edilson Serpeloni; Outa Angeleli, Aparecida Yooko; Burini, Roberto Carlos
Fonte: Soc Brasileira Med Esporte Publicador: Soc Brasileira Med Esporte
Tipo: Artigo de Revista Científica Formato: 215-220
Português
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The effect of increased protein intake on the muscle mass gain, nitrogen balance and N-15-glycine kinetics was studied in six young, healthy subjects practitioners of strength training (> 2 years), without use of anabolic steroids and in agreement with the ethical principles of the research. All athletes received adequate diet (0.88g protein/kg/day) during 2 weeks prior the study (D1), and thereafter with diet providing 1.5g of protein/kg/day and 30kcal/g of protein (D2 diet) for the subsequent 2 weeks. Later on, they all received diet with 2.5g of protein/kg/day (D3 diet) and 30 kcal/g protein for the last two weeks. Body composition, food intake, blood biochemistry, nitrogen balance (NB) and 15N-glycine kinetics were determined at the beginning, after D1 (M0) and in the last days of the D2 (M1) and D3 (M2). The results showed at the end of the study (4 weeks) significant increase in muscle mass (1.63 +/- 0.9kg), without difference between D2 and D3. The NB followed the protein/energy consumption (M0 = -7.8g/day; M1 = 5.6g/day and D3 = 16.6g/day), the protein synthesis followed the NB, with M0 < (M1= M2) (M1 = 49.8 +/- 12.2g N/day and M2 = 52.5 +/- 14.0g N/day). Protein catabolism rate was similarly kept among diets. Thus, the results of the NB and N-15-glycine kinetics indicate that the recommended protein intake for these athletes is higher than the one for sedentary adults (0.88g/kg) and lower than 2.5g/kg...

‣ Comparison of omega-3 fatty acids and sulfasalazine in ulcerative colitis

Dichi, I; Frenhane, P.; Dichi, J. B.; Correa, C. R.; Angeleli, AYO; Bicudo, M. H.; Rodrigues, MAM; Victoria, C. R.; Burini, R. C.
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 87-90
Português
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Fish oil omega-3 fatty acids exert antiinflammatory effects on patients with ulcerative colitis. However, a comparative study in patients with mild to moderate ulcerative colitis receiving only sulfasalazine or omega-3 fatty acids has not been performed. We sought to detect changes in the inflammatory disease activity with the use of either fish oil omega-3 fatty acids or sulfasalazine in patients with ulcerative colitis. Ten patients (five male, five female; mean age = 48 +/- 12 y) with mild to moderate active ulcerative colitis were investigated in a randomized cross-over design. They received either sulfasalazine (2 g/d) or omega-3 fatty acids (5.4 g/d) for 2 mo. Disease activity was assessed by clinical and laboratory indicators, sigmoidoscopy, histology, and whole-body protein turnover (with N-15-glycine). Treatment with w-3 fatty acids resulted in greater disease activity as detected by a significant increase in platelet count, erythrocyte sedimentation rate, C-reactive protein, and total fecal nitrogen excretion. No major changes in protein synthesis and breakdown were observed during either treatment. In conclusion, treatment with sulfasalazine is superior to treatment with omega-3 fatty acids in patients with mild to moderate active ulcerative colitis. Nutrition 2000;16:87-901 (C) Elsevier B.V. 2000.

‣ Protein-energy status and N-15-glycine kinetic study of Child A cirrhotic patients fed low- to high-protein energy diets

Dichi, I; Dichi, J. B.; PapiniBerto, S. J.; Angeleli, AYO; Bicudo, M. H.; Rezende, T. A.; Burini, R. C.
Fonte: Elsevier B.V. Publicador: Elsevier B.V.
Tipo: Artigo de Revista Científica Formato: 519-523
Português
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In five male cirrhotic patients (Child A) and in four age- and sex-matched healthy control subjects, whole-body protein turnover was measured using a single oral dose of N-15-glycine as a tracer and urinary ammonia as end product. Subjects were studied in the fasting and feeding state, with different levels of protein and energy intake. The patients were underweight and presented lower plasma transthyretin and retinol-binding protein levels. When compared with controls, the kinetic studies showed patients to be hypometabolic in the fasting (Do) state and with the control diet [D-1 = (0.85 g of protein/154 kJ). kg(-1). day(-1)]. However, when corrected by body weight, the kinetic differences between groups disappeared, whereas the N-retention in the feeding state showed better results for the patients due mainly to their efficient breakdown decrease. When fed high-level protein or energy diets [D-2 = (0.9 g protein/195 kJ) and D-3 = (1.56 g protein/158 kJ). kg(-1). day(-1)], the patients showed D-0 = D-1 = D-2 < D-3 for N-flux and (D-0 = D-1) < D-3 (D-2 is intermediary) for protein synthesis. Thus, the present data suggest that the remaining mass of the undernourished mild cirrhotic patients has fairly good protein synthesis activity and also that protein...

‣ Resistência de genótipos de soja Glycine max (L.) a Nezara viridula (L. ,1758) (Hemiptera: Pentatomidae)

Souza, Efrain de Santana
Fonte: Universidade Estadual Paulista (UNESP) Publicador: Universidade Estadual Paulista (UNESP)
Tipo: Dissertação de Mestrado Formato: viii, 83f.: il. color. , grafs., tabs.
Português
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Pós-graduação em Agronomia (Proteção de Plantas) - FCA; Visando avaliar a resistência de diferentes genótipos de soja [Glycine max (L.)] frente ao ataque do percevejo Nezara viridula (L.) (Hemiptera: Pentatomidae), foram conduzidos ensaios em laboratório sob condições controladas (T= 25  2º C, U.R.= 70  10% e fotoperíodo= 12 h). Foram realizados testes de atratividade e preferência para alimentação com grãos maduros e vagens verdes dos genótipos de soja „Conquista‟, „IAC-17‟, „IAC-19‟, „IAC-24‟, „PI-227687‟, „TMG-103‟, „TMG-117‟, „TMG-121‟, „BRS-242‟ e „IAC-PL1‟, avaliando-se o número de insetos atraídos aos 15, 30, 45, 60, 120 e 180 minutos após a liberação. No teste com vagens verdes, contou-se também o número de picadas e o tempo médio de alimentação por picada. Visando associar a preferência do inseto com as características físicas dos genótipos, realizou-se a contagem do número de tricomas em vagens verdes (densidade/área) e a medição da textura nestas mesmas vagens. Estudos de antibiose também foram realizados utilizando-se os mesmos genótipos, acompanhando-se diariamente o desenvolvimento dos insetos confinados a cada material (25 ninfas/genótipo). Nesta fase avaliou-se: a duração de cada estádio e a duração total da fase ninfal; a mortalidade por estádio e total da fase ninfal; o peso de ninfas (N5) e adultos recém-emergidos (24 h) e o período de desenvolvimento de ovo a adulto. Em teste com chance de escolha...

‣ Agonist action of taurine on glycine receptors in rat supraoptic magnocellular neurones: possible role in osmoregulation.

Hussy, N; Deleuze, C; Pantaloni, A; Desarménien, M G; Moos, F
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 01/08/1997 Português
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1. To evaluate the implication of taurine in the physiology of supraoptic neurones, we (i) investigated the agonist properties of taurine on glycine and GABAA receptors of supraoptic magnocellular neurones acutely dissociated from adult rats, using whole-cell voltage clamp, (ii) studied the effects of taurine and strychnine in vivo by extracellular recordings of supraoptic vasopressin neurones in anaesthetized rats, and (iii) measured the osmolarity-dependent release of endogenous taurine from isolated supraoptic nuclei by HPLC. 2. GABA, glycine and taurine evoked rapidly activating currents that all reversed close to the equilibrium potential for Cl-, indicating activation of Cl(-)-selective channels. Glycine-activated currents were reversibly blocked by strychnine (IC50 of 35 nM with 100 microM glycine), but were unaffected by the GABAA antagonist gabazine (1-3 microM). GABA-activated currents were reversibly antagonized by 3 microM gabazine, but not by strychnine (up to 1 microM). 3. Responses to 1 mM taurine were blocked by strychnine but not by gabazine and showed no additivity with glycine-induced currents, indicating selective activation of glycine receptors. Responses to 10 mM taurine were partially antagonized by gabazine...

‣ Spontaneous motoneuronal activity mediated by glycine and GABA in the spinal cord of rat fetuses in vitro.

Nishimaru, H; Iizuka, M; Ozaki, S; Kudo, N
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 15/11/1996 Português
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1. Spontaneous motoneuronal activity was monitored from the lumbar ventral roots in an isolated spinal cord preparation from rat fetuses at embryonic days (E) 13.5-18.5. 2. Spontaneous bursts that were synchronized in both left and right ventral roots were observed periodically (mean interval, 1.5-2.6 min) from E14.5 to 17.5. This activity was abolished in Ca(2+)-free saline or by application of tetrodotoxin (1 microM), indicating that it was synaptically mediated. 3. The glutamate receptor blocker kynurenate (4 mM) failed to block spontaneous bursts at E14.5-15.5, though it completely abolished them at E17.5. The glycine receptor antagonist strychnine (10 microM) completely blocked spontaneous bursts at E14.5-15.5. Bicuculline, a GABAA receptor antagonist, reduced the amplitude of the spontaneous bursts. 4. At E15.5, a brief application of glycine (250 microM to 2 mM) evoked excitatory responses resembling the spontaneous bursts in both time course and amplitude. Such glycine-induced responses were not observed under Ca(2+)-free conditions, suggesting that they were synaptically evoked. These synaptic responses were not blocked by kynurenate (4 mM), but they were abolished by strychnine (10 microM). 5. It is concluded that glycine and GABA generate the earliest spontaneous motor activity of the fetus and function transiently as excitatory transmitters in the embryonic spinal cord.

‣ Synthesis and Characterization of Amphiphilic Cyclic Diblock Copolypeptoids from N-Heterocyclic Carbene-Mediated Zwitterionic Polymerization of N-Substituted N-carboxyanhydride

Lee, Chang-Uk; Smart, Thomas P.; Guo, Li; Epps, Thomas H.; Zhang, Donghui
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em 29/11/2011 Português
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N-Heterocyclic carbene (NHC)-mediated ring-opening polymerization of N-decylN-carboxylanhydride monomer (De-NCA) has been shown to occur in a controlled manner, yielding cyclic poly(N-decyl-glycine)s (c-PNDGs) with polymer molecular weights (MW) between 4.8 and 31 kg·mol−1 and narrow molecular weight distributions (PDI < 1.15). The reaction exhibits pseudo-first order kinetics with respect to monomer concentration. The polymer MW increases linearly with conversion, consistent with a living polymerization. ESI MS and SEC analysesconfirm the cyclic architectures of the forming polymers. DSC and WAXS studies reveal that the c-PNDG homopolymers are highly crystalline with two prominent first order transitions at 72–79°C (Tm,1) and 166–177°C (Tm,2), which have been attributed to the side chain and main chain melting respectively. A series of amphiphilic cyclic diblock copolypeptoids [i.e.,poly(N-methyl-glycine)-b-poly(N-decyl-glycine) (c-PNMG-b-PNDG)] with variable molecular weight and composition was synthesized by sequential NHC-mediated polymerization of the corresponding N-methyl N-carboxyanhydride (Me-NCA) and De-NCA monomers. 1H NMR analysis reveals that adjusting the initial monomer to NHC molar ratio can readily control the block copolymer chain length and composition. Time-lapsed light scattering and cryogenic transmission electron microscopy (cryo-TEM) analysis of c-PNDG-b-PNMG samples revealed that the amphiphilic cyclic block copolypeptoids self-assemble into spherical micelles that reorganize into micron-long cylindrical micelles with uniform diameter in room temperature methanol over the course of several days. An identical morphological transition has also been noted for the linear analogs...

‣ N-Arachidonyl Glycine Does Not Activate G Protein–Coupled Receptor 18 Signaling via Canonical Pathways

Lu, Van B.; Puhl, Henry L.; Ikeda, Stephen R.
Fonte: The American Society for Pharmacology and Experimental Therapeutics Publicador: The American Society for Pharmacology and Experimental Therapeutics
Tipo: Artigo de Revista Científica
Português
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47.26973%
Recent studies propose that N-arachidonyl glycine (NAGly), a carboxylic analogue of anandamide, is an endogenous ligand of the Gαi/o protein–coupled receptor 18 (GPR18). However, a high-throughput β-arrestin–based screen failed to detect activation of GPR18 by NAGly (Yin et al., 2009; JBC, 18:12328). To address this inconsistency, this study investigated GPR18 coupling in a native neuronal system with endogenous signaling pathways and effectors. GPR18 was heterologously expressed in rat sympathetic neurons, and the modulation of N-type (Cav2.2) calcium channels was examined. Proper expression and trafficking of receptor were confirmed by the “rim-like” fluorescence of fluorescently tagged receptor and the positive staining of external hemagglutinin-tagged GPR18-expressing cells. Application of NAGly on GPR18-expressing neurons did not inhibit calcium currents but instead potentiated currents in a voltage-dependent manner, similar to what has previously been reported (Guo et al., 2008; J Neurophysiol, 100:1147). Other proposed agonists of GPR18, including anandamide and abnormal cannabidiol, also failed to induce inhibition of calcium currents. Mutants of GPR18, designed to constitutively activate receptors, did not tonically inhibit calcium currents...

‣ Efeito da germinacao de soja cv. BR-13 e Parana sobre acido fitico, fosforo total e inibidores de tripsina.

RIBEIRO, M.L.L.; IDA, E.I.; OLIVEIRA, M.C.N. de
Fonte: Pesquisa Agropecuaria Brasileira, Brasilia, v.34, n.1, p.31-36, jan. 1999. Publicador: Pesquisa Agropecuaria Brasileira, Brasilia, v.34, n.1, p.31-36, jan. 1999.
Tipo: Artigo em periódico indexado (ALICE)
Português
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Este trabalho foi conduzido para avaliar o efeito da germinacao sobre os constituintes antinutricionais da soja, acido fitico, P total e inibidores de tripsina, que interferem na utilizacao de proteinas e outras substancias. A diminuicao no aproveitamento de nutrientes resulta em inibicao de crescimento, hipoglicemia ou danos a tecidos animais. Para os ensaios foram utilizadas as cultivares de soja (Glycine max (L.) Merrill) BR-13 e Parana. As sementes foram incubadas em camara de germinacao a 25 graus C. e 100% de umidade, de 0 a 72 horas, com coleta de amostras em intervalos equidistantes de seis horas. A cultivar Parana apresentou teores superiores de acido fitico, P total e inibidores de tripsina, em relacao a cultivar Br-13. As analises de variancia e comparacoes entre medias indicaram que houve aumentos significativos (p<0.05) nos teores de P total (8,5% e 3,6% nas cultivares BR-13 e Parana, respectivamente) e nos niveis de inibidores de tripsina (22,05% e 17,10% nas cultivares BR-13 e Parana respectivamente), e reducoes nos teores de acido fitico (15,96% e 9,46% nas cultivares BR-13 e Parana, respectivamente), em 72 horas de germinacao.; 1999

‣ Diversidade genética da soja entre períodos e entre programas de melhoramento no Brasil.

PRIOLLI, R.H.G.; MENDES-JUNIOR, C.T.; SOUSA, S.M.B.; SOUSA, N.E.A.; CONTEL, E.P.B.
Fonte: Pesquisa Agropecuária Brasileira, Brasília, v.39, n.10, p.967-975, out. 2004. Publicador: Pesquisa Agropecuária Brasileira, Brasília, v.39, n.10, p.967-975, out. 2004.
Tipo: Artigo em periódico indexado (ALICE)
Português
Relevância na Pesquisa
46.863896%
O objetivo deste trabalho foi detectar os efeitos do melhoramento sobre a diversidade do germoplasma da soja cultivada nas três ultimas décadas, por meio da comparação de seis programas de melhoramento e períodos de lançamento de cultivares, utilizando locos microssatélites. Em relação aos programas de melhoramento, todos os locos apresentaram diferenças significativas em suas distribuições alélicas. Alguns locos eram compostos de alelos exclusivos em alguns programas de melhoramento, enquanto outros foram compostos sempre dos mesmos alelos em maior freqüência para todos os programas. A AMOVA indicou maior porção da variância devido a cultivares dentro de programas e somente 5,3% (p<0,05) devido à diferença entre programas. Quando comparados os programas de melhoramento entre si, cinco entre as 15 comparações apresentaram diferenças significativas (p<0,05), estando presente o programa IAC em quatro destas cinco comparações. As estimativas de variabilidade da soja entre os períodos de melhoramento avaliados indicaram que somente 1,78% da variância total foi devida à diferença entre períodos (p>0,05). Os resultados sugerem que o germoplasma de soja utilizado em programas de melhoramento no Brasil manteve nível constante de diversidade genética nos últimos 30 anos...

‣ Expressão dos genes nodC, nodW e nopP em Bradyrhizobium japonicum estirpe CPAC 15 avaliada por RT-qPCR.

BORTOLAN, S.; BARCELLOS, F. G.; MARCELINO, F. C.; HUNGRIA, M.
Fonte: Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 11, p. 1491-1498, nov. 2009 Publicador: Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 11, p. 1491-1498, nov. 2009
Tipo: Artigo em periódico indexado (ALICE)
Português
Relevância na Pesquisa
47.1268%
Resumo - O objetivo deste trabalho foi avaliar a expressão, por RT-qPCR, dos genes de nodulação nodC e nodW e do gene nopP da estirpe CPAC 15, que provavelmente atuam na infecção das raízes da soja. Foram realizados dois experimentos. No primeiro, a expressão dos genes foi avaliada nas células após a incubação com genisteína por 15 min, 1, 4 e 8 horas. Os resultados revelaram que os três genes apresentaram maior expressão imediatamente após o contato com o indutor (15 min). No segundo experimento, a bactéria foi cultivada na presença de indutores (genisteína ou exsudatos de sementes de soja) por 48 horas. A expressão dos três genes foi maior na presença de genisteína, com valores de expressão para nodC, nodW e nopP superiores ao controle. Os resultados obtidos confirmam a funcionalidade dos três genes na estirpe CPAC 15, com ênfase para o nopP, cuja funcionalidade em Bradyrhizobium japonicum foi descrita pela primeira vez.; 2009

‣ Diversidade genética da soja entre períodos e entre programas de melhoramento no Brasil.

PRIOLLI, R. H. G.; MENDES-JUNIOR, C. T.; SOUSA, S. M. B.; SOUSA, N. E. A.; CONTEL, E. P. B.
Fonte: Pesquisa Agropecuária Brasileira, Brasília, v. 39, n. 10, p. 967-975, out. 2004. Publicador: Pesquisa Agropecuária Brasileira, Brasília, v. 39, n. 10, p. 967-975, out. 2004.
Tipo: Artigo em periódico indexado (ALICE)
Português
Relevância na Pesquisa
46.92891%
O objetivo deste trabalho foi detectar os efeitos do melhoramento sobre a diversidade do germoplasma da soja cultivada nas três ultimas décadas, por meio da comparação de seis programas de melhoramento e períodos de lançamento de cultivares, utilizando locos microssatélites. Em relação aos programas de melhoramento, todos os locos apresentaram diferenças significativas em suas distribuições alélicas. Alguns locos eram compostos de alelos exclusivos em alguns programas de melhoramento, enquanto outros foram compostos sempre dos mesmos alelos em maior freqüência para todos os programas. A AMOVA indicou maior porção da variância devido a cultivares dentro de programas e somente 5,3% (p<0,05) devido à diferença entre programas. Quando comparados os programas de melhoramento entre si, cinco entre as 15 comparações apresentaram diferenças significativas (p<0,05), estando presente o programa IAC em quatro destas cinco comparações. As estimativas de variabilidade da soja entre os períodos de melhoramento avaliados indicaram que somente 1,78% da variância total foi devida à diferença entre períodos (p>0,05). Os resultados sugerem que o germoplasma de soja utilizado em programas de melhoramento no Brasil manteve nível constante de diversidade genética nos últimos 30 anos...

‣ Análise de fatores e estratificação ambiental na avaliação da adaptabilidade e estabilidade em soja

MENDONÇA, O.; CARPENTIERI-PÍPOLO, V.; GARBUGLIO, D.D.; JUNIOR, N. da S.F.
Fonte: Pesquisa Agropecuária Brasileira, Brasília, v. 42, n.11, p. 1567-1575, nov. 2007 Publicador: Pesquisa Agropecuária Brasileira, Brasília, v. 42, n.11, p. 1567-1575, nov. 2007
Tipo: Artigo em periódico indexado (ALICE)
Português
Relevância na Pesquisa
46.863896%
O objetivo deste trabalho foi avaliar a eficiência de diferentes métodos de estratificação ambiental, a representatividade dos locais de avaliação, e a adaptabilidade e estabilidade de genótipos de soja, por meio de ensaios de produtividade de grãos, nos estados do Paraná e Santa Catarina, nos anos agrícolas de 2000 a 2003, em um total de 15 ambientes. Foram utilizados, para estratificação ambiental, o método tradicional de Lin e a análise de fatores aliada ao porcentual de parte simples (PS%) da interação genótipo vs. ambiente (GxA). Na determinação da adaptabilidade e estabilidade dos genótipos, foram utilizados modelos baseados em regressão linear única e bissegmentada. Foram testados 21 genótipos de soja em delineamento de blocos ao acaso com três repetições, na avaliação da produtividade de grãos. O genótipo RB 605 apresenta ampla faixa de adaptação com elevada produtividade média de grãos. De acordo com ambos os métodos, as localidades de Palotina e Brasilândia do Sul podem ser reduzidas a somente um local de ensaio.A análise de fatores associada ao PS% da interação GxA é mais seletiva para estratificação ambiental, em relação ao método tradicional de Lin.; 2007

‣ Oxidation of oxa and thia fatty acids and related compounds catalysed by 5- and 15-lipoxygenase

Easton, C.; Robertson, T.; Pitt, M.; Rathjen, D.; Ferrante, A.; Poulos, A.
Fonte: Pergamon-Elsevier Science Ltd Publicador: Pergamon-Elsevier Science Ltd
Tipo: Artigo de Revista Científica
Publicado em //2001 Português
Relevância na Pesquisa
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The modified fatty acids, (Z,Z,Z)-(octadeca-6,9,12-trienyloxy)acetic acid, (Z,Z,Z)-(octadeca-9,12,15-trienyloxy)acetic acid, (all-Z)-(eicosa-5,8,11,14-tetraenyloxy)acetic acid, (all-Z)-(eicosa-5,8,11,14-tetraenylthio)acetic acid, 3-[(all-Z)-(eicosa-5,8,11,14-tetraenylthio)]propionic acid, (all-Z)-(eicosa-5,8,11,14-tetraenylthio)succinic acid, N-[(all-Z)-(eicosa-5,8,11,14-tetraenoyl)]glycine and N-[(all-Z)-(eicosa-5,8,11,14-tetraenoyl)]aspartic acid, all react with soybean 15-lipoxygenase. The products were treated with triphenylphosphine to give alcohols, which were isolated using HPLC. Analysis of the alcohols using negative ion tandem electrospray mass spectrometry, and by comparison with compounds obtained by autoxidation of arachidonic acid, shows that each enzyme-catalysed oxidation occurs at the omega-6 position of the substrate. In a similar fashion, it has been found that (Z,Z,Z)-(octadeca-6,9,12-trienyloxy)acetic acid, (Z,Z,Z)-(octadeca-9,12,15-trienyloxy)acetic acid, (all-Z)-(eicosa-5,8,11,14-tetraenylthio)acetic acid and 3-[(all-Z)-(eicosa-5,8,11,14-tetraenylthio)]propionic acid each undergoes regioselective oxidation at the carboxyl end of the polyene moiety on treatment with potato 5-lipoxygenase. Neither (all-Z)-(eicosa-5...

‣ Regionally different N-methyl-D-aspartate receptors distinguished by ligand binding and quantitative autoradiography of [3H]-CGP 39653 in rat brain.

Mugnaini, M.; van Amsterdam, F. T.; Ratti, E.; Trist, D. G.; Bowery, N. G.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /11/1996 Português
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37.85057%
1. Binding of D,L-(E)-2-amino-4-[3H]-propyl-5-phosphono-3-pentenoic acid ([3H]-CGP 39653), a high affinity, selective antagonist at the glutamate site of the N-methyl-D-aspartate (NMDA) receptor, was investigated in rat brain by means of receptor binding and quantitative autoradiography techniques. 2. [3H]-CGP 39653 interacted with striatal and cerebellar membranes in a saturable manner and to a single binding site, with KD values of 15.5 nM and 10.0 nM and receptor binding densities (Bmax values) of 3.1 and 0.5 pmol mg-1 protein, respectively. These KD values were not significantly different from that previously reported in the cerebral cortex (10.7 nM). 3. Displacement analyses of [3H]-CGP 39653 in striatum and cerebellum, performed with L-glutamic acid, 3-((+/-)-2-carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP) and glycine showed a pharmacological profile similar to that reported in the cerebral cortex. L-Glutamic acid and CPP produced complete displacement of specific binding with Ki values not significantly different from the cerebral cortex. Glycine inhibited [3H]CGP 39653 binding with shallow, biphasic curves, characterized by a high and a low affinity component. Furthermore, glycine discriminated between these regions (P < 0.005...

‣ Osmotic and Chill Activation of Glycine Betaine Porter II in Listeria monocytogenes Membrane Vesicles

Gerhardt, Paul N. M.; Tombras Smith, Linda; Smith, Gary M.
Fonte: American Society for Microbiology Publicador: American Society for Microbiology
Tipo: Artigo de Revista Científica
Publicado em /05/2000 Português
Relevância na Pesquisa
37.537788%
Listeria monocytogenes is a foodborne pathogen known for its tolerance to conditions of osmotic and chill stress. Accumulation of glycine betaine has been found to be important in the organism's tolerance to both of these stresses. A procedure was developed for the purification of membranes from L. monocytogenes cells in which the putative ATP-driven glycine betaine permease glycine betaine porter II (Gbu) is functional. As is the case for the L. monocytogenes sodium-driven glycine betaine uptake system (glycine betaine porter I), uptake in this vesicle system was dependent on energization by ascorbate-phenazine methosulfate. Vesicles lacking the gbu gene product had no uptake activity. Transport by this porter did not require sodium ion and could be driven only weakly by artificial gradients. Uptake rates could be manipulated under conditions not affecting secondary transport but known to affect ATPase activity. The system was shown to be both osmotically activated and cryoactivated. Under conditions of osmotic activation, the system exhibited Arrhenius-type behavior although the uptake rates were profoundly affected by the physical state of the membrane, with breaks in Arrhenius curves at approximately 10 and 18°C. In the absence of osmotic activation...

‣ Caesium ions: a glycine-activated channel agonist in rat spinal cord neurones grown in cell culture.

Smith, S. M.; McBurney, R. N.
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /04/1989 Português
Relevância na Pesquisa
37.719287%
1. The chloride (Cl-) currents activated by caesium ions (Cs+), glycine and gamma-aminobutyric acid (GABA) were compared following their application to rat neurones that had been grown in cell culture. Recordings were made using the whole-cell patch clamp technique under voltage clamp conditions. 2. In spinal cord neurones, bicuculline methiodide antagonized GABA-activated currents more effectively than Cs+ - or glycine-activated currents. However, strychnine was more effective at blocking the currents activated by Cs+ or glycine than those activated by GABA. 3. Of the 3 agonists, only GABA activated currents in cells from the intermediate lobe of the rat pituitary. 4. In spinal neurones the size of the currents activated by 70 mM Cs+ was correlated to the size of the currents activated by 15 microM glycine (P less than 0.005; n = 10, Spearman's rank correlation), but there, was no significant correlation between the size of the currents activated by these agents and 10 microM GABA. 5. The joint application of glycine and Cs+ activated currents that were approximately twice as big as the sums of the currents activated by separate applications of the same doses. This synergism was consistent with Cs+ acting at the same receptor as glycine (7 microM glycine being equivalent to 31 +/- 7 mM Cs+). 6. It was concluded that Cs+ activates the same Cl- channel as the inhibitory neurotransmitter glycine.

‣ Oxidation of oxa and thia fatty acids and related compounds catalyzed by 5- and 15-lipoxygenase

Easton, Christopher; Robertson, Thomas; Pitt, Michael; Rathjen, Deborah A; Ferrante, Antonio; Poulos, Alf
Fonte: Pergamon-Elsevier Ltd Publicador: Pergamon-Elsevier Ltd
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
37.904863%
The modified fatty acids, (Z,Z,Z)-(octadeca-6,9,12-trienyloxy)acetic acid, (Z,Z,Z)-(octadeca-9,12,15-trienyloxy)acetic acid, (all-Z)-(eicosa-5,8,11,14-tetraenyloxy)acetic acid, (all-Z)-(eicosa-5,8,11,14-tetraenylthio)acetic acid, 3-[(all-Z)-(eicosa-5,8,11,14-tetraenylthio)]propionic acid, (all-Z)-(eicosa-5,8,11,14-tetraenylthio)succinic acid, N-[(all-Z)-(eicosa-5,8,11,14-tetraenoyl)]glycine and N-[(all-Z)-(eicosa-5,8,11,14-tetraenoyl)]aspartic acid, all react with soybean 15-lipoxygenase. The products were treated with triphenylphosphine to give alcohols, which were isolated using HPLC. Analysis of the alcohols using negative ion tandem electrospray mass spectrometry, and by comparison with compounds obtained by autoxidation of arachidonic acid, shows that each enzyme-catalysed oxidation occurs at the ω-6 position of the substrate. In a similar fashion, it has been found that (Z,Z,Z)-(octadeca-6,9,12-trienyloxy)acetic acid, (Z,Z,Z)-(octadeca-9,12,15-trienyloxy)acetic acid, (all-Z)-(eicosa-5,8,11,14-tetraenylthio)acetic acid and 3-[(all-Z)-(eicosa-5,8,11,14-tetraenylthio)]propionic acid each undergoes regioselective oxidation at the carboxyl end of the polyene moiety on treatment with potato 5-lipoxygenase. Neither (all-Z)-(eicosa-5...