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‣ Tendências da incidência e da mortalidade por câncer de cólon em residentes no município de São Paulo; Trends in colon cancer incidence and mortality among residents of São Paulo

Marcolin, Marilande
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 18/12/2009 Português
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Introdução - Estudos sobre o câncer de cólon mostram que a sua incidência, no mundo, tem aumentado de maneira significativa no último século. Acredita-se que este resultado esteja relacionado, entre outros aspectos, com a industrialização, a urbanização ocorridas neste período e mudanças no estilo de vida. A morbimortalidade associada ao câncer de cólon observada em países desenvolvidos é maior do que em países em desenvolvimento e o que se tem observado é que, embora a tendência da incidência seja crescente para ambos os sexos, a mortalidade permanece estável. Objetivo - Analisar as tendências da incidência e da mortalidade de pacientes com câncer de cólon, registrados no Registro de Câncer de Base Populacional (RCBP) do Município de São Paulo. Métodos - Foram analisadas as tendências temporais da incidência no período de 1997 a 2005 e da mortalidade no período de 1980 a 2007. As análises foram feitas separadamente por sexo e faixa etária e os efeitos da idade, do período e da coorte foram estimados através do modelo de regressão de Poisson. Resultados - Houve aumento na incidência por câncer de cólon no município de São Paulo, em quase todas as faixas etárias estudadas. O aumento da mortalidade foi menor do que o aumento da incidência e parece coincidir com um efeito de coorte presente durante todo o período do estudo. Tanto na incidência quanto na mortalidade...

‣ Evaluation of chemotherapeutic potential of natural extracts using 3D models of colon cancer

Silva, Inês Alexandra Marreiros
Fonte: Faculdade de Ciências e Tecnologia Publicador: Faculdade de Ciências e Tecnologia
Tipo: Dissertação de Mestrado
Publicado em //2013 Português
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Dissertation to obtain master degree in Biotechnology; Recently there is a growing interest in cancer treatment through the use of natural compounds. In particular, phenolic compounds and monoterpenes found in fruits and vegetables are very attractive in the prevention and chemotherapy of various types of cancer. However, many promising compounds previously tested in in vitro cell models fail to demonstrate activity when evaluated in vivo. Therefore, there is an emerging need to develop more robust and reliable cellular models for pre-clinical evaluation of new chemotherapeutic agents. The main goal of this thesis was the evaluation of the chemotherapeutic potential of natural extracts, rich in bioactive compounds, using 3D models of colon cancer. For this purpose, a 3D model of human colorectal cancer cell line HT29 was developed. By culturing HT29 cells in a stirred culture system, it was possible to obtain 3D cellular spheroids with different size diameter during culture time. It was verified phenotypic changes within the spheroid along culture, such as formation of apoptotic core and altered expression of stem and epithelial markers in different spheroid areas, which are typical features of tumor progression. After an initial screening of the antiproliferative potential of 14 natural extracts performed in a 2D model of HT29 cells...

‣ Prognostic significance of microsatellite instability determined by immunohistochemical staining of MSH2 and MLH1 in sporadic T3N0M0 colon cancer

Parc, Y; Gueroult, S; Mourra, N; Serfaty, L; Fléjou, J-F; Tiret, E; Parc, R
Fonte: Copyright 2004 by Gut Publicador: Copyright 2004 by Gut
Tipo: Artigo de Revista Científica
Publicado em /03/2004 Português
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Background: Microsatellite instability (MSI) has been identified as a factor with good prognosis and chemosensitivity in stage III C colon cancer. The purpose of this study was to evaluate the routine use of immunohistochemical analysis (immunohistochemical staining of MSH2 and MLH1) to identify T3N0M0 (stage II) colon cancer with MSI and assess the prognostic value of this analysis. The study was conducted in a large cohort of patients in a single institution who had a curatively resected T3N0M0 colon cancer and were not receiving adjuvant therapy.

‣ Leptin stimulates the proliferation of human colon cancer cells in vitro but does not promote the growth of colon cancer xenografts in nude mice or intestinal tumorigenesis in ApcMin/+ mice

Aparicio, T; Kotelevets, L; Tsocas, A; Laigneau, J-P; Sobhani, I; Chastre, E; Lehy, T
Fonte: Copyright 2005 by Gut Publicador: Copyright 2005 by Gut
Tipo: Artigo de Revista Científica
Publicado em /08/2005 Português
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Background and aims: Leptin, the product of the ob gene, has been suggested to increase the risk of colon cancer. However, we have shown that although leptin stimulates epithelial cell proliferation it reduces the development of carcinogen induced preneoplastic lesions in the rat colon. Here, we explored the effect of leptin in vitro on proliferation of human colon cancer cells, and in vivo on the growth of HT-29 xenografts in nude mice and the development of intestinal tumours in ApcMin/+ mice.

‣ Using real-time impedance-based assays to monitor the effects of fibroblast-derived media on the adhesion, proliferation, migration and invasion of colon cancer cells

Dowling, Catríona M; Herranz Ors, Carmen; Kiely, Patrick A
Fonte: Portland Press Publicador: Portland Press
Tipo: info:eu-repo/semantics/article; all_ul_research; ul_published_reviewed
Português
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peer-reviewed; Increasing our knowledge of the mechanisms regulating cell proliferation, migration and invasion are central to understanding tumour progression and metastasis. The local tumour microenvironment contributes to the transformed phenotype in cancer by providing specific environmental cues that alter the cells behaviour and promotes metastasis. Fibroblasts have a strong association with cancer and in recent times there has been some emphasis in designing novel therapeutic strategies that alter fibroblast behaviour in the tumour microenvironment. Fibroblasts produce growth factors, chemokines and many of the proteins laid down in the ECM (extracellular matrix) that promote angiogenesis, inflammation and tumour progression. In this study, we use a label-free RTCA (real-time cell analysis) platform (xCELLigence) to investigate how media derived from human fibroblasts alters cancer cell behaviour. We used a series of complimentary and novel experimental approaches to show HCT116 cells adhere, proliferate and migrate significantly faster in the presence of media from human fibroblasts. As well as this, we used the xCELLigence CIMplates system to show that HCT116 cells invade matrigel layers aggressively when migrating towards media derived from human fibroblasts. These data strongly suggest that fibroblasts have the ability to increase the migratory and invasive properties of HCT116 cells. This is the first study that provides real-time data on fibroblast-mediated migration and invasion kinetics of colon cancer cells.

‣ Nutrient and antioxidant modulation of apoptosis in gastric and colon cancer cells

Matthews, G.; Howarth, G.; Butler, R.
Fonte: Landes Bioscience Publicador: Landes Bioscience
Tipo: Artigo de Revista Científica
Publicado em //2006 Português
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Gastric cancer and colon cancer are major causes of mortality and morbidity worldwide. Many cancers manifest due to changes in gene expression, particularly those involved in cellular proliferation and apoptosis. Apoptosis is an important process that removes damaged or deleterious cells and contributes to normal cellular and tissue homeostasis. Apoptosis is a tightly regulated process mediated by caspases, and the involvement of the Bcl-2 superfamily of membrane bound proteins, among others. Thus, the therapeutic induction of apoptosis has been proposed as a novel method to eliminate cancer cells. The oxidative pentose pathway (OPP) and the glutathione (GSH) antioxidant defense system play an important role in the regulation of cell growth and apoptosis. The OPP regulates intracellular redox status and provides NADPH for the synthesis of GSH, an important antioxidant. GSH is required to inactivate intracellular reactive oxygen species (ROS) which induce apoptosis and cell injury. Depletion of GSH increases the sensitivity of cells to ROS. Many chemotherapeutic agents induce apoptosis through ROS-mediated cell damage. Therefore, we speculate that the therapeutic inhibition of the OPP and/or the GSH defense system may increase the sensitivity of gastric and colon cancer cells to anti-cancer therapy. Moreover...

‣ Australian and New Zealand study comparing laparascopic and open surgeries for colon cancer in adults: Organization and conduct

Allardyce, R.; Bagshaw, P.; Frampton, C.; Frizelle, F.; Hewett, P.; Rieger, N.; Smith, S.; Solomon, M.; Stevenson, A.
Fonte: Blackwell Science Asia Publicador: Blackwell Science Asia
Tipo: Artigo de Revista Científica
Publicado em //2008 Português
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This article describes the initiation and implementation of the multicentre Australia and New Zealand prospective randomized controlled clinical study comparing laparoscopic and conventional open surgical treatments of right-sided and left-sided potentially curable colon cancer (Australasian Laparoscopic Colon Cancer Study). Six hundred and one adult patients were admitted with a clinical diagnosis of a single adenocarcinoma based on a physical examination and colonoscopy, barium enema or computed tomography scan and randomly allocated to either laparoscopic or open surgery. The primary aim of the study is to compare 5-year mortality and tumour recurrence rates between the two groups. Secondary aims include comparisons of safety (intraoperative and early postoperative complications, wound site recurrence, postoperative recovery and 30-day mortality), quality of life, in-hospital costs and short-term mortality and tumour recurrence. The data for 592 patients have been collected. There are currently 3141 person years of follow up. In all 370 patients have been assessed at 5 years. This study shows that large cooperative Australia–New Zealand surgical trials can and should be carried out to address significant clinical issues. When possible...

‣ General practice vs surgical-based follow-up for patients with colon cancer: randomised controlled trial

Wattchow, D.; Weller, D.; Esterman, A.; Pilotto, L.; McGorm, K.; Hammett, Z.; Platell, C.; Silagy, C.
Fonte: Nature Publishing Group Publicador: Nature Publishing Group
Tipo: Artigo de Revista Científica
Publicado em //2006 Português
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This trial examined the optimal setting for follow-up of patients after treatment for colon cancer by either general practitioners or surgeons. In all, 203 consenting patients who had undergone potentially curative treatment for colon cancer were randomised to follow-up by general practitioners or surgeons. Follow-up guidance recommended three monthly clinical review and annual faecal occult blood tests (FOBT) and were identical in both study arms. Primary outcome measures (measured at baseline, 12 and 24 months were (1) quality of life, SF-12; physical and mental component scores, (2) anxiety and depression: Hospital Anxiety and Depression Scale and (3) patient satisfaction: Patient Visit-Specific Questionnaire. Secondary outcomes (at 24 months) were: investigations, number and timing of recurrences and deaths. In all, 170 patients were available for follow-up at 12 months and 157 at 24 months. At 12 and 24 months there were no differences in scores for quality of life (physical component score, P=0.88 at 12 months; P=0.28 at 24 months: mental component score, P=0.51, P=0.47; adjusted), anxiety (P=0.72; P=0.11) depression (P=0.28; P=0.80) or patient satisfaction (P=0.06, 24 months). General practitioners ordered more FOBTs than surgeons (rate ratio 2.4...

‣ Long-term outcomes of the Australasian randomized clinical trial comparing laparoscopic and conventional open surgical treatments for colon cancer: The Australasian Laparoscopic Colon Cancer Study Trial

Bagshaw, P.; Hewett, P.; Rieger, N.
Fonte: Lippincott Williams & Wilkins Publicador: Lippincott Williams & Wilkins
Tipo: Artigo de Revista Científica
Publicado em //2012 Português
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OBJECTIVE: We report a multicentered randomized controlled trial across Australia and New Zealand comparing laparoscopic-assisted colon resection (LCR) with open colon resection (OCR) for colon cancer. BACKGROUND: Colon cancer is a significant worldwide health issue. This trial investigated whether the short-term benefits associated with LCR for colon cancer could be achieved safely, without survival disadvantages, in our region. METHODS: A total of 601 patients with potentially curable colon cancer were randomized to receive LCR or OCR. Primary endpoints were 5-year overall survival, recurrence-free survival, and freedom from recurrence rates, compared using an intention-to-treat analysis. RESULTS: On April 5, 2010, 587 eligible patients were followed for a median of 5.2 years (range, 1 week–11.4 years) with 5-year confirmed follow-up data for survival and recurrence on 567 (96.6%). Significant differences between the 2 trial groups were as follows: LCR patients were older at randomization, and their pathology specimens showed smaller distal resection margins; OCR patients had some worse pathology parameters, but there were no differences in disease stages. There were no significant differences between the LCR and OCR groups in 5-year follow-up of overall survival (77.7% vs 76.0%...

‣ Short-chain fatty acids induce apoptosis in colon cancer cells associated with changes to intracellular redox state and glucose metabolism

Matthews, G.; Howarth, G.; Butler, R.
Fonte: Karger Publicador: Karger
Tipo: Artigo de Revista Científica
Publicado em //2012 Português
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BACKGROUND: Short-chain fatty acids (SCFA) are undergoing increased scrutiny as chemotherapeutics for colon cancer, although a comprehensive understanding of their mode of action is lacking. We investigated candidate SCFA for their capability to modulate apoptosis, cell cycle, intracellular redox state and glucose metabolism in the Caco-2 human colon cancer cell line. METHODS: Caco-2 cells were incubated with butyrate, propionate or a combination of these SCFA (1:1) and assessed by flow cytometry, enzyme activity analysis or by isotope ratio mass spectrometry. RESULTS: Butyrate and the SCFA combination induced apoptosis and G2-M arrest to a greater extent than propionate alone (p < 0.05). SCFA treatment led to time-dependent alterations to the oxidative pentose pathway, reductions in glutathione availability and increases in levels of reactive oxygen species (p < 0.05) compared with untreated controls. The rate of D-glucose metabolism was increased by all SCFA, although to the greatest extent by butyrate (p < 0.05). CONCLUSIONS: These results suggest that butyrate, or the combination of both SCFA, induced rapid and extensive apoptosis and G2-M arrest associated with changes to redox state and D-glucose metabolism. These results support the potential for butyrate and propionate to act as adjuncts to conventional chemotherapy regimens for colon cancer.; Matthews G.M....

‣ The intestinal epithelial cell differentiation marker intestinal alkaline phosphatase (ALPi) is selectively induced by histone deacetylase inhibitors (HDACi) in colon cancer cells in a Kruppel-like Factor 5 (KLF5)-defendent manner

Shin, J.; Carr, A.; Corner, G.A.; Tögel, L.; Dávaos-Salas, M.; Tran, H.; Chueh, A.C.; Al-Obaidi, S.; Chionh, F.; Ahmed, N.; Buchanan, D.D.; Young, J.P.; Malo, M.S.; Hodin, R.A.; Arango, D.; Sieber, O.M.; Augenlicht, L.H.; Dhillon, A.S.; Weber, T.K.; Mar
Fonte: American Society for Biochemistry and Molecular Biology Publicador: American Society for Biochemistry and Molecular Biology
Tipo: Artigo de Revista Científica
Publicado em //2014 Português
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The histone deacetylase inhibitor (HDACi) sodium butyrate promotes differentiation of colon cancer cells as evidenced by induced expression and enzyme activity of the differentiation marker intestinal alkaline phosphatase (ALPi). Screening of a panel of 33 colon cancer cell lines identified cell lines sensitive (42%) and resistant (58%) to butyrate induction of ALP activity. This differential sensitivity was similarly evident following treatment with the structurally distinct HDACi, MS-275. Resistant cell lines were significantly enriched for those harboring the CpG island methylator phenotype (p = 0.036, Chi square test), and resistant cell lines harbored methylation of the ALPi promoter, particularly of a CpG site within a critical KLF/Sp regulatory element required for butyrate induction of ALPi promoter activity. However, butyrate induction of an exogenous ALPi promoter-reporter paralleled up-regulation of endogenous ALPi expression across the cell lines, suggesting the presence or absence of a key transcriptional regulator is the major determinant of ALPi induction. Through microarray profiling of sensitive and resistant cell lines, we identified KLF5 to be both basally more highly expressed as well as preferentially induced by butyrate in sensitive cell lines. KLF5 overexpression induced ALPi promoter-reporter activity in resistant cell lines...

‣ Molekulare Zusammenhänge der Retinoid-Insensitivität der humanen Kolonkarzinomzellinie HCT116; Molecular conditions of retinoic insensitivity by analyzing the human colon cancer cell line HCT 116

Raff, Corinna
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
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Molekulare Zusammenhänge der Retinoid-Insensitivität der humanen Kolon-karzinomzellinie HCT116 Corinna Raff, Sektion für Strahlenbiologie, Abteilung für Radioonkologie, Eberhard-Karls-Universität Tübingen HINTERGRUND: Retinoide spielen aufgrund ihrer antiproliferativen, differenzie-rungsinduzierenden und radiosensitivierenden Wirkung eine wichtige Rolle bei der Behandlung von bösartigen Tumoren. Da der genaue Mechanismus der Zyto- und Radiotoxizität nicht vollständig geklärt ist, wurde die retinoidresistente humane Kolonkarzinomzellinie HCT 116 untersucht, um Aufschlüsse über die Ursache der Resistenz und den Wirkmechanismus zu erhalten. METHODIK: Mittels Koloniebildungstests wurde eine radiosensitivierende Wirkung der Retinoide untersucht. Des weiteren wurde die basale und die durch Behandlung mit all-trans- bzw. 13-cis-Retinsäure induzierte Expression der Bindungsproteine CRABP I und II sowie der nukleären Retinoidrezeptoren RARalpha, beta, gamma und RXRalpha und beta untersucht. Diese Parameter wurden mit Hilfe von RT-PCR, ELISA und Western Blot erhoben. Durch eine Doppelstrang-Sequenzierung wurde die Gensequenz des Bindungsproteins CRABP II und des Retinoidrezeptors RARbeta untersucht. ERGEBNISSE und DISKUSSION: Die Kolonkarzinomzellinie HCT116 ist eine retin-säureresistente Zellinie...

‣ Membrane androgen receptor activation triggers pro-apoptotic responses in vitro and in vivo and blocks migration in colon cancer; Membrane Androgen-Rezeptor-Aktivierung auslöst pro-apoptotische Reaktionen in vitro und in vivo und blockiert die Migration bei Darmkrebs

Gu, Shuchen
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
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The classical intracellular androgen receptors (iAR) mediate genomic androgen signals, which take at least more than half an hour. However, the rapid or non-genomic action of androgens takes only seconds to few minutes and involves the activation of androgen membrane binding sites. Although the molecular identity of those membrane binding sites remains still unknown, their expression has been reported in many cell types, including various tumor cells. Activation of membrane androgen receptors (mAR) in prostate and breast cancer cells has been implicated in the regulation of cell growth, motility and apoptosis. Here we analyzed mAR expression and function in colon cancer. Using fluorescent mAR ligands we showed specific membrane staining in mouse colon tumor tissues and in iAR silenced Caco2 cell lines. Stimulation of colon-mAR by testosterone-albumin-conjugates induced rapid actin and tubulin cytoskeleton reorganization and generated apoptotic responses, even in the presence of anti-androgens. We showed that long-term activation of mAR in Caco2 cell lines down-regulated the activity of PI-3K and Akt and induced de-phosphorylation/activation of the pro-apoptotic Bad. Treatment of APCmin/+ mice significantly decreased the expression of p-AKT and p-Bad levels in tumor tissue. Moreover...

‣ The natural triterpene maslinic acid induces apoptosis in HT29 colon cancer cells by a JNK-p53-dependent mechanism

Reyes Zurita, Fernando Jes??s; Pach??n Pe??a, Gisela; Liz??rraga, Daneida; Rufino Palomares, Eva Encarnaci??n; Cascante Serratosa, Marta; Lupi????ez Cara, Jos?? Antonio
Fonte: Biomed Central Publicador: Biomed Central
Tipo: Artigo de Revista Científica
Português
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[Background] Maslinic acid, a pentacyclic triterpene found in the protective wax-like coating of the leaves and fruit of Olea europaea L., is a promising agent for the prevention of colon cancer. We have shown elsewhere that maslinic acid inhibits cell proliferation to a significant extent and activates mitochondrial apoptosis in colon cancer cells. In our latest work we have investigated further this compound's apoptotic molecular mechanism. [Methods] We used HT29 adenocarcinoma cells. Changes genotoxicity were analyzed by single-cell gel electrophoresis (comet assay). The cell cycle was determined by flow cytometry. Finally, changes in protein expression were examined by western blotting. Student's t-test was used for statistical comparison. [Results] HT29 cells treated with maslinic acid showed significant increases in genotoxicity and cell-cycle arrest during the G0/G1 phase after 72 hours' treatment and an apoptotic sub-G0/G1 peak after 96 hours. Nevertheless, the molecular mechanism for this cytotoxic effect of maslinic acid has never been properly explored. We show here that the anti-tumoral activity of maslinic acid might proceed via p53-mediated apoptosis by acting upon the main signaling components that lead to an increase in p53 activity and the induction of the rest of the factors that participate in the apoptotic pathway. We found that in HT29 cells maslinic acid activated the expression of c-Jun NH2-terminal kinase (JNK)...

‣ Transcriptional changes in human Caco-2 colon cancer cells following exposure to a recurrent non-toxic dose of polyphenol-rich chokeberry juice

Bermúdez-Soto, María J.; Larrosa, Mar; Garcia-Cantalejo, Jesús M.; Espín de Gea, Juan Carlos; Tomás Barberán, Francisco; García-Conesa, María Teresa
Fonte: Springer Publicador: Springer
Tipo: Artículo Formato: 259768 bytes; application/pdf
Português
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3 pages.-- Printed version published Oct 2007.; Berries and red fruits are important dietary sources of polyphenols [1]. In vitro and animal studies have demonstrated the bioavailability and the anti-proliferative and anticarcinogenic properties of these fruits or of their phenolic components [2, 3]. Consumption of berries may contribute to the reduction of colon cancer by mechanisms not yet understood. Gene expression analysis using microarrays allows for a more comprehensive study of the possible molecular mechanisms by which food or food components may prevent certain cancers of the gastrointestinal tract [4]. The aim of this research is to investigate the anti-proliferative effects of a polyphenol-rich berry juice on a human model of colon cancer cells and its association to transcriptional changes in relation to colon cancer.; Peer reviewed

‣ Conhecimento de mulheres sobre câncer de mama e de colo do útero; Women' knowledge about breast cancer and colon cancer

Silva, Nancy Capretz Batista da; Franco, Maria Aparecida Paiva; Marques, Susi Lippi
Fonte: Universidade de São Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto Publicador: Universidade de São Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; Formato: application/pdf
Publicado em 01/12/2005 Português
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No Brasil, a incidência do câncer de mama e do colo do útero está altamente relacionada à falta de informação. É importante avaliar o conhecimento da população feminina sobre o assunto e, a partir disso, estabelecer estratégias para a diminuição das ocorrências. Assim, este trabalho visou avaliar o conhecimento de 294 mulheres- funcionárias de hospital, escolas e professoras- com idade entre 20 e 57 anos, sobre este tema, por meio de teste objetivo e questionário. Os dados revelaram que embora 89,11% delas procurava adotar condutas preventivas, ainda há dúvidas sobre formas de incidência e prevenção do câncer de mama e colo do útero. Aponta-se a viabilidade e aplicabilidade do instrumento (teste objetivo) como recurso técnico para planejamento de intervenções que ampliem o conhecimento sobre a doença e mudem a atitude da população na prevenção e detecção precoce do câncer de mama e colo do útero no âmbito da Saúde Pública.; In Brazil, breast cancer and colon cancer incidences are highly related to the lack of information. It is important to evaluate the knowledge that the feminine population has about this matter to establish strategies in order to decrease those events. In this context, the purpose of this study was to evaluate the knowledge that 294 women (hospitals...

‣ Reduction of b-Glucuronidase and nitroreductase activity by yoghurt in a murine colon cancer model

de Moreno de LeBlanc,A.; Perdigón,G.
Fonte: Biocell Publicador: Biocell
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2005 Português
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Yoghurt feeding inhibits induced colon cancer in mice. Several studies showed the immunomodulatory effect of yoghurt which can explain this inhibition. It is possible that yoghurt bacteria can also affect gut flora enzymes related to colon carcinogenesis as reported for other probiotics in different animal tumours. The aim of this work was to evaluate the role of yoghurt starter bacteria and their cell-free fermentation products on the reduction of procarcinogen enzyme activities (beta-glucuronidase and nitroreductase). Mice injected with 1,2-dimethylhydrazine (DMH) and fed with yoghurt were used for this study. Mice given milk or yoghurt supernatant (cell free extract) were used to evaluate if the yoghurt antitumour effect is due to the starter bacteria or other components released during fermentation, that could inhibit these enzymes. We determined that yoghurt by itself maintained enzymes activities similar or lower than nontreatment control group, and the enzyme activity was also lower than milk or yoghurt supernatant groups. DMH increased the activity of the enzymes. Mice injected with DMH and fed cyclically with yoghurt presented lower enzymes activities than the tumour control group. Feeding yoghurt decreased procarcinogenic enzyme levels in the large intestine contents of mice bearing colon tumour. The results of this study provide another mechanism by which yoghurt starter bacteria interact with the large intestine of the mice and prevent colon cancer.

‣ The value of abdominal ultrasound in the diagnosis of colon cancer

Martínez-Ares,D.; Martín-Granizo Barrenechea,I.; Souto-Ruzo,J.; Yáñez López,J.; Pallarés Peral,A.; Vázquez-Iglesias,J. L.
Fonte: Revista Española de Enfermedades Digestivas Publicador: Revista Española de Enfermedades Digestivas
Tipo: info:eu-repo/semantics/article; journal article; info:eu-repo/semantics/publishedVersion Formato: text/html; application/pdf
Publicado em 01/12/2005 Português
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Introduction: colon cancer is one of the main causes of cancer death. Diagnosis requires the examination of the entire large bowel by means of radiological or endoscopic techniques. Many patients suspect of colon cancer are referred for colonoscopy but nevertheless this suspicion is not confirmed after endoscopic examination. The objective of this study is the evaluation of the reliability of abdominal ultrasound in the diagnosis of these tumors. Material and method: we selected patients suspect of colon cancer referred to the endoscopy unit for a colonoscopy. An abdominal ultrasound was carried out on all patients prior to the endoscopy. Considering the endoscopic examination as a gold standard, the sensibility, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the ultrasonography were evaluated. Likewise, a series of analytical and clinical parameters were evaluated, in an attempt to establish associated factors of a colon cancer. The statistical analysis was carried out by means of the statistical package SPSS 12.0 for Windows. Results: 145 patients were included in the study (56.6% males) with an average 66.72 years of age (22-89). A cancer was diagnosed in 42 cases (28.9%). In the diagnosis of colon cancer...

‣ Impact of the total number of harvested lymph nodes after colon cancer resections on survival in patients without involved lymph node

Rivadulla-Serrano,M. I.; Martínez-Ramos,D.; Armengol-Carrasco,M.; Escrig-Sos,J.; Daroca-José,J. M.; Paiva-Coronel,G. A.; Fortea-Sanchís,C.; Salvador-Sanchis,J. L.
Fonte: Revista Española de Enfermedades Digestivas Publicador: Revista Española de Enfermedades Digestivas
Tipo: info:eu-repo/semantics/article; journal article; info:eu-repo/semantics/publishedVersion Formato: text/html; application/pdf
Publicado em 01/05/2010 Português
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Background: the total number of harvested lymph nodes has been demonstrated to be of prognostic significance for colon cancer. Differences can occur in the total number of harvested lymph nodes between different specialists (surgeons and pathologists). Objective: the aim of this study was to analyse if, in our centre, the number of analysed lymph nodes in patients with colon cancer that are classified as pN0 is also related to survival. Material and methods: a retrospective study was designed, where 148 patients with colon adenocarcinoma (pN0 of TNM classification) who underwent elective surgery between 1 January 1995 and 31 December 2001, with curative intent were included. Three groups were created according to the number of analysed lymph nodes (< 7, 7-14, > 14 lymph nodes). For survival analysis the Kaplan-Meier and CUSUM curves methods were used. Results: the total number of analysed lymph nodes was 1,493 (mean 10.1 lymph nodes per patient). The rate of 5-years survival was 63.0% in the group with < 7 lymph nodes; 7-14 lymph nodes: 80.6% and those with > 14 lymph nodes: 91.8% (p < 0.01). Prognostic significance was also present for multivariate analysis. Conclusion: in our centre, harvesting a larger number of lymph nodes is related to improved rates of 5-years survival for patients with colon cancer staged as pN0. It seems reasonable to recommend obtaining as many lymph nodes as possible...

‣ The effect of combining interferon-α and gefitinib in human colon cancer cell lines

Yang,Li; Wang,Fang; He,Fang; Huang,Li Ya
Fonte: West Indian Medical Journal Publicador: West Indian Medical Journal
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/03/2011 Português
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BACKGROUND AND AIMS: Interferon-α (IFN-α) treatment is associated with up-regulation of epidermal growth factor receptor (EGFR) expression and marked growth inhibition of colon cancer cell lines. We aimed to determine the effect of combining IFN-α and gefitinib in the growth of human colon cancer cell lines. METHODS: Two human colon cancer cell lines SW480 and LOVO were treated with IFN-α alone or gefitinib alone or IFN-α plus gefitinib. Proliferation of colon cancer cells was measured by methyl thiazolyl tetrazolium (MTT) assay; the apoptosis rate was analysed by flow cytometry (FCM). The expression of XIAP, XAF1 mRNA was detected by RT-PCR and the expression of XIAP, XAF1 protein was detected by western blotting. RESULTS: Methyl thiazolyl tetrazolium showed that IFN-α, gefitinib and IFN-α plus gefitinib significantly inhibited SW480 and LOVO cells in a dose-dependent manner (p < 0.05). The FCM revealed that IFN α, gefitinib and IFN-α plus gefitinib could markedly upgrade the apoptosis rate (p < 0.05). The expression of XIAP mRNA down-regulated markedly (p < 0.05) while the expression of XAF1 mRNA up-regulated significantly (p < 0.05). The expression of XIAP protein was down-regulated markedly (p < 0.05) while the expression of XAF1 protein was up-regulated significantly (p < 0.05). CONCLUSION: IFN-α promotes the antiproliferaative effect of gefitinib on human colon cancer cell lines and the mechanism may be related to up-regulation expression of EGFR by IFN-α.