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‣ Desenvolvimento de uma técnica molecular para detecção e quantificação do vírus da hepatite G (GBV-C/HGV); The Hepatitis G (GBV-C / HGV) agent is a flavivirus

Silva, Synara Alexandre Araujo
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 01/06/2010 Português
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Introdução: O vírus da hepatite G (GBV-C/HGV) é um flavivírus de genoma RNA de fita simples polaridade positiva, replicando em linfócitos, não sendo até hoje associado a qualquer patogenia humana. Estudos recentes demonstram que pessoas co-infectadas pelos vírus GBV-C/HGV e HIV têm menor progressão para AIDS e morte, embora alguns estudos tenham falhado em demonstrar tais efeitos. Objetivo: Determinar a soroprevalência e viremia (qualitativa) por GBV-C/HGV nas amostras de pacientes HIV+ e desenvolver uma metodologia de PCR em tempo real para fazer a determinação das cargas virais de GBV-C/HGV. Métodos: Avaliamos a presença de anticorpo e RNA do vírus GBV-C/HGV em 253 amostras de plasma de mulheres HIV positivas, coletadas entre 1997-99. Realizamos o ensaio imunoenzimático anti-E2 (EIA anti-HGenv kit, Roche(TM)), a reação em cadeia da polimerase (PCR), o NESTED PCR e, padronizamos um PCR em tempo real (RT-PCR), ensaio baseado no sistema Taqman, também aplicado nas mesmas amostras. A curva-padrão do ensaio foi feita com diluições seriadas de uma bolsa de plasma GBV-C/HGV+, e os resultados expressos em unidades aleatórias/mL. Resultados: Das 253 amostras testadas, 64 foram positivas para o anticorpo anti-E2 (25...

‣ Hepatitis G virus / GB virus C in Brazil. Preliminary report

Pinho,J.R.R.; Capacci,M.L.; Silva,L.C. da; Carrilho,F.J.; Santos,C.A.; Pugliese,V; Guz,B; Levi,J.E.; Ballarati,C.A.F.; Bernardini,A.P.
Fonte: Instituto de Medicina Tropical Publicador: Instituto de Medicina Tropical
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/1996 Português
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Hepatitis G virus/ GB virus C is a novel flavivirus recently detected in hepatitis non A-E cases. In this study, the presence of this virus in chronic non-B, non-C hepatitis patients was evaluated using GBV-C specific PCR and this virus was detected in one out of thirteen patients. This patient has presented a severe liver failure, has lived for a long time in the Western Amazon basin and no other cause for this clinical picture was reported. The impact of the discovery of this new agent is still under evaluation throughout the world. The study of the prevalence of this virus among chronic hepatitis patients and healthy individuals (as blood donors) will furnish subside to evaluate its real pathogenicity.

‣ GB virus C/Hepatitis G virus and other putative hepatitis non A-E viruses

Pinho,João Renato Rebello; Silva,Luiz Caetano da
Fonte: Instituto de Medicina Tropical Publicador: Instituto de Medicina Tropical
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/1996 Português
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The identification of the major agents causing human hepatitis (Hepatitis A, B, C, D and E Viruses) was achieved during the last 30 years. These viruses are responsible for the vast majority of human viral hepatitis cases, but there are still some cases epidemiologically related to infectious agents without any evidence of infection with known virus, designated as hepatitis non A - E. Those cases are considered to be associated with at least three different viruses: 1 - Hepatitis B Virus mutants expressing its surface antigen (HBsAg) with altered epitopes or in low quantities; 2 - Another virus probably associated with enteral transmitted non A-E hepatitis, called Hepatitis F Virus. Still more studies are necessary to better characterize this agent; 3 - Hepatitis G Virus or GB virus C, recently identified throughout the world (including Brazil) as a Flavivirus responsible for about 10% of parenteral transmitted hepatitis non A-E. Probably still other unknown viruses are responsible for human hepatitis cases without evidence of infection by any of these viruses, that could be called as non A-G hepatitis.

‣ Liver histology in co-infection of hepatitis C virus (HCV) and Hepatitis G virus (HGV)

STRAUSS,Edna; GAYOTTO,Luiz Carlos da Costa; FAY,Fabian; FAY,Oscar; FERNANDES,Helena Sabino; CHAMONE,Dalton de Alencar Fischer
Fonte: Instituto de Medicina Tropical Publicador: Instituto de Medicina Tropical
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2002 Português
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As little is known about liver histology in the co-infection of hepatitis C virus (HCV) and hepatitis G virus (HGV), HGV RNA was investigated in 46 blood donors with hepatitis C, 22 of them with liver biopsy: co-infection HCV / HGV (n = 6) and HCV isolated infection (n = 16). Besides staging and grading of inflammation at portal, peri-portal and lobular areas (Brazilian Consensus), the fibrosis progression index was also calculated. All patients had no symptoms or signs of liver disease and prevalence of HGV / HCV co-infection was 15.2%. Most patients had mild liver disease and fibrosis progression index, calculated only in patients with known duration of infection, was 0.110 for co-infection and 0.130 for isolated HCV infection, characterizing these patients as "slow fibrosers". No statistical differences could be found between the groups, although a lesser degree of inflammation was always present in co-infection. In conclusion co-infection HCV / HGV does not induce a more aggressive liver disease, supporting the hypothesis that HGV is not pathogenic.

‣ Prevalence and genotypes of GB Virus C/Hepatitis G virus among blood donors in Central Brazil

Oliveira,Luciana A; Martins,Regina MB; Carneiro,Megmar AS; Teles,Sheila A; Silva,Simonne A; Cardoso,Divina DP; Lampe,Elisabeth; Yoshida,Clara FT
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2002 Português
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A survey was conducted in a blood donor population of Central Brazil aiming to investigate the prevalence of GB virus C (GBV-C)/hepatitis G virus (HGV) infection and also to analyze the virus genotypes distribution. A total of 241 voluntary blood donors were interviewed at the State Blood Bank in Goiânia, State of Goiás, Brazil. Blood samples were collected and serum samples tested for GBV-C/HGV RNA by polymerase chain reaction. Genotypes were determined by restriction fragment length polymorphism (RFLP) analysis. Seventeen samples were GBV-C/HGV RNA-positive, resulting in a prevalence of 7.1% (95% CI: 4.2-11.1). A significant trend of GBV-C/HGV RNA positivity in relation to age was observed, with the highest prevalence in donors between 29-39 years old. Ten infected individuals were characterized by reporting parenteral (30%), sexual (18%), both (6%) and intrafamiliar (6%) transmission. However, 7 (40%) GBV-C/HGV RNA-positive donors did not mention any potential transmission route. RFLP analysis revealed the presence of genotypes 1 and 2 of GBV-C/HGV; more precisely, 10 (58.9%) samples were found belonging to the 2b subtype, 4 (23.5%) to the 2a subtype, and 3 (17.6%) to genotype 1. The present data indicate an intermediate endemicity of GBV-C/HGV infection among this blood donor population...

‣ GB virus C/hepatitis G virus infection in dialysis patients and kidney transplant recipients in central Brazil

Ramos Filho,Ramon; Carneiro,Megmar AS; Teles,Sheila A; Dias,Márcia A; Cardoso,Divina DP; Lampe,Elisabeth; Yoshida,Clara FT; Martins,Regina MB
Fonte: Instituto Oswaldo Cruz, Ministério da Saúde Publicador: Instituto Oswaldo Cruz, Ministério da Saúde
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2004 Português
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In order to investigate the prevalence of GB virus C (GBV-C)/hepatitis G virus (HGV) infection in dialysis patients and kidney transplant recipients in Central Brazil and also to analyze the virus genotypes distribution, a total of 123 patients including 98 on hemodialysis, 13 on continuous ambulatory peritoneal dialysis treatment, and 12 who received kidney transplantation were interviewed in one unit of dialysis treatment in Goiânia city. Blood samples were collected and serum samples tested for GBV-C/HGV RNA by polymerase chain reaction. Genotypes were determined by restriction fragment length polymorphism (RFLP) analysis. Eighteen samples were GBV-C/HGV RNA-positive, resulting in an overall prevalence of 14.6% (95% CI: 9.2-21.7). A high positivity for GBV-C/HGV RNA was observed in patients who had received kidney transplant (16.7%), followed by those on hemodialysis (15.3%), and peritoneal dialysis (7.7%). RFLP analysis revealed the presence of genotypes 1, 2, and 3 of GBV-C/HGV; more precisely, 9 (50%) samples were found belonging to the 2b subtype, 4 (22%) to the 2a subtype, 3 (17%) to genotype 1, and 2 (11%) to genotype 3. The present data indicate an intermediate prevalence of GBV-C/HGV infection among dialysis patients and kidney transplant recipients in Central Brazil. Genotype 2 (subtype 2b) seems to be the most prevalent GBV-C/HGV genotype in our region.

‣ Hepatic histology in hepatitis C virus carriers coinfected with hepatitis G virus

Petrik, J; Guella, L; Wight, D; Pearson, G; Hinton, J; Parker, H; Allain, J; Alexander, G
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /01/1998 Português
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Background—A novel flavivirus has been described recently and designated hepatitis G virus (HGV). The virus is transmitted by the parenteral route but it is uncertain whether it is associated with chronic liver disease because liver biopsy is difficult to justify in this group. 
Aims—To examine histological features of liver biopsy in patients infected with hepatitis C virus (HCV) according to the presence or absence of HCV and HGV RNA. 
Methods—One hundred and thirty one consecutive HCV carriers undergoing staging liver biopsy were studied retrospectively. In each, HCV RNA and HGV RNA were detected by reverse transcription polymerase chain reaction on serum samples collected at the time of biopsy. The presence of each RNA was correlated with histological features blind to the RNA results; individual histological features of inflammation or fibrosis were scored separately. 
Results—Nineteen patients were positive for both HGV and HCV RNA in serum, 91 were positive for HCV RNA alone, two were positive for HGV RNA alone, and 19 were negative for both RNA species. Neither age nor sex differed between the groups; a greater proportion of intravenous drug users were HGV RNA positive, but this was not statistically significant. There was no effect of HGV coinfection on the stage of fibrosis or any other histological parameter except steatosis; patients with HCV and HGV RNA had a higher mean score for fat than those patients with HCV RNA alone (p<0.05). 
Conclusions—HGV coinfection has no important effects on histological features in chronic HCV carriers. It is unlikely that HGV infection causes chronic liver disease. 



‣ Hepatitis G virus genomic RNA is pathogenic to Macaca mulatta

Ren, Hao; Zhu, Fen-Lu; Cao, Ming-Mei; Wen, Xin-Yu; Zhao, Ping; Qi, Zhong-Tian
Fonte: Baishideng Publishing Group Inc Publicador: Baishideng Publishing Group Inc
Tipo: Artigo de Revista Científica
Português
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AIM: To explore the pathogenicity and infectivity of hepatitis G virus (HGV) by observing replication and expression of the virus, as well as the serological and histological changes of Macaca mulatta infected with HGV genomic RNA or HGV RNA-positive serum.

‣ High prevalence of GB Virus C/Hepatitis G Virus RNA among Brazilian blood donors

Levi,José Eduardo; Contri,Daniela Gazoto; Lima,Liliam Pereira; Takaoka,Deise Tihe; Garrini,Regina Helena; Santos,Wellingtom; Fachini,Roberta; Wendel,Silvano
Fonte: Instituto de Medicina Tropical Publicador: Instituto de Medicina Tropical
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2003 Português
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The aim of this study was to investigate the presence of the Hepatitis G Virus on a population of blood donors from São Paulo, Brazil and to evaluate its association to sociodemographic variables. Two RT-PCR systems targeting the putative 5'NCR and NS3 regions were employed and the former has shown a higher sensitivity. The observed prevalence of HGV-RNA on 545 blood donors was 9.7% (CI 95% 7.4;12.5). Statistical analysis depicted an association with race/ethnicity, black and mulatto donors being more frequently infected; and also with years of education, less educated donors presenting higher prevalences. No association was observed with other sociodemographic parameters as age, gender, place of birth and of residence. DNA sequencing of nine randomly chosen isolates demonstrated the presence of genotypes 1, 2 and 3 among our population but clustering of these Brazilian isolates was not detected upon phylogenetic analysis.

‣ Reverse transcription-PCR detection of hepatitis G virus.

Schlueter, V; Schmolke, S; Stark, K; Hess, G; Ofenloch-Haehnle, B; Engel, A M
Fonte: PubMed Publicador: PubMed
Tipo: Artigo de Revista Científica
Publicado em /11/1996 Português
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Hepatitis G virus (HGV) was recently identified as a new member of the Flaviviridae, but its clinical significance is still unclear. Since no immunoassay for the diagnosis of HGV is available, we developed a sensitive reverse transcription-PCR (RT-PCR) assay to facilitate the detection of the viral genome by mass screening in the clinical laboratory. Sequences within the 5'-noncoding region and within the putative NS5a region are independently amplified in the presence of digoxigenin-11-dUTP and are detected by hybridization with biotinylated capture probes binding to a streptavidin-coated matrix. Semiquantitative Enzymun-Test DNA detection via chemiluminescence can be performed either in a microtiter plate format or on fully automated ES 300 machines. We were able to detect at least 8 x 10(2) genome equivalents per ml of serum using both primer pairs. HGV was shown to be present in 43 of 130 (33%) serum samples from intravenous drug abusers with a high risk of parenteral exposure. However, only two of the patients were positive when the NS5a primers only were used, and only one patient was positive when only the 5'-noncoding region primers were used, demonstrating the increased sensitivity of HGV detection with two sets of primers. Among these patients...

‣ Perturbations induced by synthetic peptides from hepatitis G virus structural proteins in lipid model membranes: a fluorescent approach

Larios, Cristina; Casas, Jordi; Mestres, Concepció; Haro Villar, Isabel; Alsina, M. Asunción
Fonte: John Wiley & Sons Publicador: John Wiley & Sons
Tipo: Artículo Formato: 22195 bytes; application/pdf
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3 pages, 2 figures.-- PMID: 16134194 [PubMed].-- Printed version published in issue Jul-Oct 2005.-- Issue title: "Proceedings of the XIth International Symposium on Luminescence Spectrometry - Detection Techniques in Biomedical and Environmental Analysis - (ISLS 2004). Part 2" (Beijing, China, Sep 22-24, 2004).; The name HGV/GBV-C remains as an acronym for hepatitis G virus (HGV) and GB virus-C (GBV-C), strain variants of this enveloped RNA virus independently but simultaneously discovered in 1995. Nowadays there is no evidence that it causes hepatitis in humans either during initial infection or after long-term carriage, but it has been recently related with HIV regarding the inhibition of progression to AIDS.; The overall genomic organization of HGV/GBV-C is similar to that of hepatitis C virus (HCV) and other members of the Flavivirus family in Hepacivirus genus. Although a stretch of conserved, hydrophobic amino acids within the envelop glycoprotein of HCV has been proposed as the virus fusion peptide, the mode of entry of GBV-C/HGV into target cells is at present unknown. In the present work, sequences derived from the structural E2-protein of HGV/GBV-C have been selected by means of semiempirical methods and then synthesized manually following solid-phase methodologies. Their ability to induce perturbations in model membranes has been analysed by measuring the penetration of such peptides in lipid monolayers and by a series of experiments based on tryptophan peptide fluorescence emission spectra. Besides...

‣ Effects of overlapping GB virus C/hepatitis G virus synthetic peptides on biomembrane models

Larios, Cristina; Busquets, M. Antònia; Carilla Auguet, Josep; Alsina, M. Asunción; Haro Villar, Isabel
Fonte: American Chemical Society Publicador: American Chemical Society
Tipo: Artículo Formato: 22195 bytes; application/pdf
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12 pages, 8 figures, 6 tables.-- PMID: 15568870 [PubMed].-- Printed version published Dec 7, 2004.; The present study was undertaken to examine the physicochemical properties of three overlapping peptides belonging to the E2 envelope protein of Hepatitis G virus (GBV-C/HGV) and its interaction with phospholipid biomembrane models using biophysical techniques. We describe our findings concerning the surface activity and the interaction of the peptides with monolayers and liposomes composed of the zwitterionic phospholipids dipalmitoylphosphatidylcholine and dimyristoylphosphatidylcholine (DMPC) and a mixture of DMPC with the anionic phospholipid dimyristoylphosphatidylglycerol. The results inform about the effect of the chain length on their interaction with biomembrane models. The longest chain peptide interacts in a higher extent with all the phospholipid studied as a result of a combination of hydrophobic and electrostatic forces.; This work was funded by Grants BQU2003-05070-CO2-01/02 from the Ministerio de Ciencia y Tecnología (Spain).; Peer reviewed

‣ Influence of the saturation chain and head group charge of phospholipids in the interaction of hepatitis G virus synthetic peptides

Pérez, S.; Miñones Jr., J.; Espina, Marta; Alsina, M. Asunción; Haro Villar, Isabel; Mestres, Concepció
Fonte: American Chemical Society Publicador: American Chemical Society
Tipo: Artículo Formato: 22195 bytes; application/pdf
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10 pages, 11 figures, 3 tables.-- PMID: 16853582 [PubMed].-- Printed version published Oct 27, 2005.; Using the Langmuir technique, we have studied the properties at the air/water interface and the interaction of the hepatitis G virus synthetic peptide E1(53−66) and its palmitoyl derivative with membrane phospholipids. These phospholipids had different characteristics referring to the net charge and saturation of the acyl chain. The palmitoyl derivative was more stable at the air/water interface and in the kinetic at constant area measurements showed higher incorporation to the monolayer. The interaction was higher for saturated phospholipids and those with a negative net charge. When the peptides were in the subphase, they produced changes in the miscibility of mixed monolayers composed of DPPC/DPPG or DOPC/DOPG. It can be deduced from the results obtained that electrostatic interactions play a major role, but when the peptide is derivatized with the palmitoyl chain, hydrophobic interactions are added to the former ones. The interaction is also influenced by the saturation of the acyl chain.; This work was supported by project BQU2003-0507-CO2-01/02, from the Ministerio de Ciencia y Tecnología (Spain).; Peer reviewed

‣ Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles

Larios, Cristina; Christiaens, Bart; Gómara Elena, María José; Alsina, M. Asunción; Haro Villar, Isabel
Fonte: Blackwell Publishing Publicador: Blackwell Publishing
Tipo: Artículo Formato: 22195 bytes; application/pdf
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11 pages, 7 figures, 2 tables.-- PMID: 15885095 [PubMed].-- Printed version published May 2005.; The interaction with phospholipid bilayers of two synthetic peptides with sequences corresponding to a segment next to the native N-terminus and an internal region of the E2 structural hepatitis G virus (HGV/GBV-C) protein [E2(7–26) and E2(279–298), respectively] has been characterized. Both peptides are water soluble but associate spontaneously with bilayers, showing higher affinity for anionic than zwitterionic membranes. However, whereas the E2(7–26) peptide is hardly transferred at all from water to the membrane interface, the E2(279–298) peptide is able to penetrate into negatively charged bilayers remaining close to the lipid/water interface. The nonpolar environment clearly induces a structural transition in the E2(279–298) peptide from random coil to α-helix, which causes bilayer perturbations leading to vesicle permeabilization. The results indicate that this internal segment peptide sequence is involved in the fusion of HGV/GBV-C to membrane.; This work was funded by grants BQU2003-05070-CO2-01/02 from the Ministerio de Ciencia y Tecnología (Spain) and a predoctoral grant awarded to C. L.; Peer reviewed

‣ Characterization of a putative fusogenic sequence in the E2 hepatitis G virus protein

Larios, Cristina; Casas, Jordi; Alsina, M. Asunción; Mestres, Concepció; Gómara Elena, María José; Haro Villar, Isabel
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 22195 bytes; application/pdf
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11 pages, 8 figures, 2 tables.-- PMID: 16165082 [PubMed].-- Printed version published Oct 15, 2005.; Supporting information available at: http://dx.doi.org/10.1016/j.abb.2005.06.027; With the aim of better understanding the fusion process mediated by the envelope proteins of the hepatitis G virus (HGV/GBV-C), we have investigated the interaction with model membranes of two overlapping peptides [(267–284) and (279–298)] belonging to the E2 structural protein. The peptides were compared for their ability to perturb lipid bilayers by means of different techniques such as differential scanning calorimetry and fluorescence spectroscopy. Furthermore, the conformational behaviour of the peptides in different membrane environments was studied by Fourier-transform infrared spectroscopy and circular dichroism. The results showed that only the E2(279–298) peptide sequence was able to bind with high affinity to negatively charged membranes, to permeabilize efficiently negative lipid bilayers, to induce haemolysis, and to promote inter-vesicle fusion. This fusogenic activity could be related to the induced peptide conformation upon interaction with the target membrane.; This work was supported by Project BQU2003-0507-C02-01/02 from the Ministerio de Ciencia y Tecnología (Spain).; Peer reviewed

‣ Study in mono and bilayers of the interaction of hepatitis G virus (GBV-C/HGV) synthetic antigen E2(99-118) with cell membrane phospholipids

Dastis, Macarena; Rojo, Nuria; Alsina, M. Asunción; Haro Villar, Isabel; Panda, Amiya Kumar; Mestres, Concepció
Fonte: Elsevier Publicador: Elsevier
Tipo: Artículo Formato: 22195 bytes; application/pdf
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11 pages, 7 figures, 1 table.-- PMID: 15110935 [PubMed].-- Printed version published Jun 1, 2004.; The interaction of the hepatitis G synthetic peptide E2(99-118) with cell membrane phospholipids of different characteristics such as dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) was studied by Langmuir isotherms. Epifluorescence microscopy and Atomic force microscopy (AFM) was also used to study interactions with DPPC. Compression isotherms of DPPC/E2(99-118) and DPPG/E2(99-118) mixed monolayers showed negative deviation from ideallity consistent with the existence of attractive interactions. The incorporation of the peptide in DPPC monolayer was also confirmed in epifluorescence microscopy and AFM studies. The peptide retarded the formation of DPPC domains and did not let the phospholipid get organized. No important differences in the interactions with DPPC (neutral) or DPPG (anionic) were found, thus suggesting that electrostatics forces do not have a predominant influence in these interactions.; This work was supported by a BOYCAST fellowship of the Department of Science and Technology, Govt.of India awarded to A.K. Panda and grants BQU2000-0793-C02-01 /02 from CICYT (Spain).; Peer reviewed

‣ Vírus da Hepatite G / Vírus GB-C no Brasil; Hepatitis G virus / GB virus C in Brazil. Preliminary report

Pinho, J.R.R.; Capacci, M.L.; Silva, L.C. da; Carrilho, F.J.; Santos, C.A.; Pugliese, V; Guz, B; Levi, J.E.; Ballarati, C.A.F.; Bernardini, A.P.
Fonte: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo Publicador: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/06/1996 Português
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O vírus da Hepatite G ou vírus GB-C é um novo vírus recentemente descoberto em casos de hepatites não A-E. Neste estudo, casos de hepatite crônica não-B, não-C foram testados com uma reação de amplificação específica para GBV-C. Este vírus foi detectado em 1 entre os 13 casos estudados. Este paciente apresentava insuficiência hepática severa, tinha habitado por vários anos na Amazônia Ocidental e nenhuma outra causa para este quadro clínico havia sido relatada. O impacto da descoberta deste novo agente está ainda sendo investigado. O estudo da prevalência deste vírus entre pacientes com hepatite crônica e entre indivíduos sadios (como, por exemplo, doadores de sangue) fornecerá subsídios para o estabelecimento de sua real patogenicidade.; Hepatitis G virus/ GB virus C is a novel flavivirus recently detected in hepatitis non A-E cases. In this study, the presence of this virus in chronic non-B, non-C hepatitis patients was evaluated using GBV-C specific PCR and this virus was detected in one out of thirteen patients. This patient has presented a severe liver failure, has lived for a long time in the Western Amazon basin and no other cause for this clinical picture was reported. The impact of the discovery of this new agent is still under evaluation throughout the world. The study of the prevalence of this virus among chronic hepatitis patients and healthy individuals (as blood donors) will furnish subside to evaluate its real pathogenicity.

‣ Histologia hepática na co-infecção do vírus da hepatite C (VHC) e vírus da hepatite G (VHG); Liver histology in co-infection of hepatitis C virus (HCV) and Hepatitis G virus (HGV)

STRAUSS, Edna; GAYOTTO, Luiz Carlos da Costa; FAY, Fabian; FAY, Oscar; FERNANDES, Helena Sabino; CHAMONE, Dalton de Alencar Fischer
Fonte: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo Publicador: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/04/2002 Português
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As escassas informações sobre histologia hepática na co-infecção do vírus da Hepatite C (VHC) e vírus da Hepatite G (VHG) nos levou a investigar o RNA-VHG em 46 doadores de sangue com hepatite C, dos quais 22 com biópsia hepática: co-infecção VHC / VHG (n = 6) e infecção isolada do VHC (n = 16). Além de estadiamento e gradação da atividade inflamatória nas áreas portal, peri-portal e lobular, segundo o Consenso Brasileiro, calculamos também o índice de progressão da fibrose. Os pacientes estudados não apresentavam sintomas ou sinais físicos de doença hepática. A prevalência da co-infecção VHC / VHG foi de 15,2%. A maior parte dos pacientes apresentava-se com lesão hepática discreta e o índice de progressão da fibrose, calculado apenas nos pacientes com duração conhecida da infecção, foi de 0,110 para os co-infectados e de 0,130 para aqueles com infecção isolada pelo VHC, caracterizando esses pacientes como "fibrosantes lentos". Não foram encontradas diferenças estatísticas entre os grupos, apesar de menor grau de inflamação em todas as áreas analisadas, nos casos de co-infecção. Em conclusão, a co-infecção VHC / VHG não induz o surgimento de lesão hepática mais grave, favorecendo a hipótese de que o VHG não é patogênico.; As little is known about liver histology in the co-infection of hepatitis C virus (HCV) and hepatitis G virus (HGV)...

‣ Alta prevalência do RNA do vírus da hepatite G (HGV) em doadores de sangue brasileiros; High prevalence of GB Virus C/Hepatitis G Virus RNA among Brazilian blood donors

Levi, José Eduardo; Contri, Daniela Gazoto; Lima, Liliam Pereira; Takaoka, Deise Tihe; Garrini, Regina Helena; Santos, Wellingtom; Fachini, Roberta; Wendel, Silvano
Fonte: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo Publicador: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/04/2003 Português
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O objetivo deste estudo foi investigar a presença do vírus da hepatite G e sua associação com variáveis sóciodemográficas em uma população de doadores de sangue da cidade de São Paulo, Brasil. Dois sistemas de PCR foram empregados, um focando a região 5' não-codificadora e outro a região não-estrutural 3, apresentando o primeiro maior sensibilidade. A prevalência detectada do HGV-RNA em 545 doadores foi de 9,7% (IC 95% 7,4;12,5). A prevalência do HGV-RNA foi significativamente maior em doadores da raça negra/mulata (26,5%) quando comparados aos doadores caucasianos (7,5%), (OR = 4,5). Também foi observada uma tendência de maiores prevalências em doadores de menor escolaridade. Não se observou associação significativa com outros parâmetros estudados como idade, sexo, origem geográfica e local de residência. O sequenciamento do DNA de nove isolados selecionados ao acaso demonstrou a presença dos genótipos 1, 2 e 3, mas não houve um agrupamento das amostras brasileiras quando submetidas à análise filogenética; The aim of this study was to investigate the presence of the Hepatitis G Virus on a population of blood donors from São Paulo, Brazil and to evaluate its association to sociodemographic variables. Two RT-PCR systems targeting the putative 5'NCR and NS3 regions were employed and the former has shown a higher sensitivity. The observed prevalence of HGV-RNA on 545 blood donors was 9.7% (CI 95% 7.4;12.5). Statistical analysis depicted an association with race/ethnicity...

‣ O vírus da Hepatite G e outros possíveis vírus causadores de hepatites não-A, não-E; GB virus C/Hepatitis G virus and other putative hepatitis non A-E viruses

Pinho, João Renato Rebello; Silva, Luiz Caetano da
Fonte: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo Publicador: Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
Tipo: info:eu-repo/semantics/article; info:eu-repo/semantics/publishedVersion; ; ; ; ; Formato: application/pdf
Publicado em 01/12/1996 Português
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A identificação dos virus responsáveis pela vasta maioria dos casos de hepatite (Vírus das Hepatites A, B, C, D e E) foi realizada durante os últimos 30 anos. Entretanto, existem ainda alguns casos epidemiologicamente relacionados com agentes infecciosos, nos quais não se encontra nenhuma evidência de infecção por nenhum vírus conhecido. Estes casos foram designados de Hepatites não A-E e são atualmente relacionados com pelo menos três diferentes vírus: 1 - Mutantes do Vírus da Hepatite B, que expressam o antígeno de superfície (AgHBs) com epitopos alterados ou em baixas concentrações. 2 - Um outro vírus, denominado Vírus da Hepatite F, foi associado com as hepatites não A-E de transmissão entérica. Entretanto, maiores estudos são ainda necessários para sua melhor caracterização. 3 - O Vírus da Hepatite G ou Vírus GB-C foi recentemente identificado em diferentes regiões do mundo (incluindo o Brasil) como um Flavi-vírus responsável por cerca de 10% dos casos de hepatites não A-E de transmissão parenteral. Provavelmente, ainda outros vírus responsáveis pelas hepatites humanas serão encontrados em casos de hepatites sem marcadores de infecção por nenhum destes vírus.; The identification of the major agents causing human hepatitis (Hepatitis A...