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‣ Sinais de hipervascularização em imagens de ressonância magnética em metástases ósseas de carcinoma de células renais; Signs of hypervascularization at magnetic resonance imaging in bone metastases from renal cell carcinoma

FELTRIN, Leonir Terezinha; FERREIRA, José Renato; MAMERE, Augusto Elias; COELHO, Rafael Darahem de Souza; CECIN, Alexandre Oliveira; LUCCHESI, Fabiano Rubião; PINHEIRO, Marco Antônio Lopes; TRAD, Clóvis Simão
Fonte: Colégio Brasileiro de Radiologia e Diagnóstico por Imagem Publicador: Colégio Brasileiro de Radiologia e Diagnóstico por Imagem
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
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OBJETIVO: Avaliar a frequência de hipervascularização pela visualização de vasos no interior ou ao redor de metástases ósseas de carcinoma de células renais. MATERIAIS E MÉTODOS: Foram avaliados, retrospectivamente, exames de ressonância magnética de 13 pacientes com diagnóstico de carcinoma de células renais, com 15 lesões ósseas metastáticas, que não haviam sido submetidos a nenhum tratamento. RESULTADOS: Foram encontrados sinais de hipervascularização em 12 das 15 lesões (80%), sendo 6 na coluna lombar, 3 na bacia, 1 na coluna torácica, 1 na ulna e 1 na tíbia. CONCLUSÃO: A alta frequência de hipervascularização em metástases ósseas de carcinoma de células renais encontrada neste trabalho pode sugerir a etiologia renal, tornando-se muito útil na apresentação clínica usual de lesão óssea única com neoplasia primária desconhecida.; OBJECTIVE: To evaluate the frequency of hypervascularization by visualizing vascular structures inside or around bone metastases from renal cell carcinoma. MATERIALS AND METHODS: Magnetic resonance imaging studies of 13 untreated patients with diagnosis of renal cell carcinoma and 15 metastatic bone lesions were retrospectively evaluated. RESULTS: Signs of hypervascularization were found in 12 of the 15 bone lesions (80%)...

‣ Renal Cell Carcinoma: T1 and T2 Signal Intensity Characteristics of Papillary and Clear Cell Types Correlated with Pathology

OLIVA, M. Raquel; GLICKMAN, Jonathan N.; ZOU, Kelly H.; TEO, Sze Y.; MORTELE, Koenraad J.; ROCHA, Manoel S.; SILVERMAN, Stuart G.
Fonte: AMER ROENTGEN RAY SOC Publicador: AMER ROENTGEN RAY SOC
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
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OBJECTIVE. The objective of our study was to describe the T1 and T2 signal intensity characteristics of papillary renal cell carcinoma (RCC) and clear cell RCC with pathologic correlation. MATERIALS AND METHODS. Of 539 RCCs, 49 tumors (21 papillary RCCs and 28 clear cell RCCs) in 45 patients were examined with MRI. Two radiologists retrospectively and independently assessed each tumor`s T1 and T2 signal intensity qualitatively and quantitatively (i.e., the signal intensity [SI] ratio [tumor SI/renal cortex SI]). Of the 49 tumors, 37 (76%) were assessed for pathology features including tumor architecture and the presence of hemosiderin, ferritin, necrosis, and fibrosis. MRI findings and pathology features were correlated. Statistical methods included summary statistics and Wilcoxon`s rank sum test for signal intensity, contingency tables for assessing reader agreement, concordance rate between the two readers with 95% CIs, and Fisher`s exact test for independence, all stratified by RCC type. RESULTS. Papillary RCCs and clear cell RCCs had a similar appearance and signal intensity ratio on T1-weighted images. On T2-weighted images, most papillary RCCs were hypointense (reader 1, 13/21; reader 2, 14/21), with an average mean signal intensity ratio for both readers of 0.67 +/- 0.2...

‣ Análise do perfil de hipermetilação do gene PTEN e correlação com fatores clínicos anatomopatológicos no carcinoma de células renais; Analysis of hypermethylation profile of PTEN gene and correlation with clinical and pathological findings in renal cell carcinoma

Campos, Eurico Cleto Ribeiro de
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 02/08/2011 Português
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Introdução: Apesar da identificação de fatores prognósticos clínicos e patológicos, muitos pacientes portadores de carcinoma de células renais (CCR) apresentam metástases ao diagnóstico e outros irão desenvolver recorrência local ou à distância durante o seguimento. Novos fatores prognósticos e de origem molecular têm sido avaliados no CCR, destacando-se o PTEN como um dos principais genes envolvidos na carcinogênese renal. Objetivos: Avaliar os fatores clínicos e anatomopatológicos mais significativos nas taxas de sobrevida, identificar a frequência de hipermetilação do gene PTEN através da técnica do pirosequenciamento, o impacto da hipermetilação do gene nas taxas de sobrevida global (SG) e livre de doença (SLD), como também, a associação da presença de hipermetilação com os principais fatores prognóticos. Material e métodos: Foram avaliados 137 pacientes portadores de CCR submetidos a tratamento cirúrgico do tumor primário entre 1997 e 2009. Foram considerados os dados epidemiológicos, clínicos, anatomopatológicos, de estadiamento (TNM 2004) e os obtidos da reação de pirosequenciamento. Resultados: O tempo de seguimento médio foi de 32,3 meses e mediana de 28,8 meses. Considerando o estadimento clínico...

‣ Expressão de Ciclina D1 em Carcinoma de Células Renais; Expression of Cyclin D1 in Renal Cell Carcinoma

Lima, Marcela Sampaio
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Dissertação de Mestrado Formato: application/pdf
Publicado em 12/06/2013 Português
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Carcinoma de Células Renais (CCR) representa uma família de tumores distintos com evolução clínica imprevisível. Uma variedade de moléculas tem sido avaliada como marcadores prognósticos para CCR. Ciclina D1, uma proteína reguladora do ciclo celular, encontra-se superexpressa em vários tumores primários. Nosso objetivo é avaliar sua expressão como marcador prognóstico em CCR. Antes disso, traçamos um perfil clínico e histopatológico da amostra e verificamos sua relação com os fatores prognósticos considerados clássicos pela literatura. 109 espécimes de pacientes diagnosticados com CCR foram obtidos entre 2005 e 2010 no Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto USP e submetidos à análise imunoistoquímica juntamente com 07 amostras de tecido renal normal. A maior parte das características epidemiológicas e clínicas de nossa amostra foi similar àquelas descritas na literatura mundial. Houve predomínio do gênero masculino, da raça branca, com idade próxima a 60 anos, frequência de pacientes assintomáticos em torno de 36% e grande prevalência do CCR de células claras (71,55%). A mortalidade específica da doença foi de 13,76%, sendo o CCR de células claras o tipo mais frequente entre os óbitos e casos metastáticos. Os casos que exibiram má evolução clínica...

‣ Renal cell carcinoma presenting as a cervical mass

Pompeo,Antonio C.; Kanashiro,Hideki; Silva,Matheus N.
Fonte: Sociedade Brasileira de Urologia Publicador: Sociedade Brasileira de Urologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2005 Português
Relevância na Pesquisa
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The authors report a case of a 60-year-old woman presenting with a renal cell carcinoma in which the first sign leading to its diagnosis was a cervical metastasis, an uncommon site of distant disease in renal neoplasms. The patient had an 18-month history of a progressively enlarging cervical mass at the anterior aspect of the neck. After laboratory and radiological evaluation, the cervical mass was excised, and the microscopic and immunohistochemical patterns suggested the possibility of a metastatic renal cell carcinoma. Computerized tomography of the abdomen showed a solid, 4 cm left renal mass. A radical left nephrectomy was performed, and the histology confirmed the suspected diagnosis. The patient received immunotherapy, and in a follow-up period of 9 months, there was no evidence of recurrent disease. It seems that head and neck metastasis of renal cell carcinoma should preferentially be treated with surgical excision because of the associated morbidity and quality-of-life issues.

‣ Lack of association between matrix metalloproteinase-1 (MMP-1) promoter polymorphism and risk of renal cell carcinoma

Piccoli,Michelly F.; Figueira,Marcia; Andreoni,Cassio; Marumo,Julio T.; Schor,Nestor; Bellini,Maria H.
Fonte: Sociedade Brasileira de Urologia Publicador: Sociedade Brasileira de Urologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/10/2007 Português
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OBJECTIVE: Investigate the possible association of insertion/deletion (2G/G) polymorphism at nucleotide -1607 of the MMP-1 promoter with the development and progression of renal cancer MATERIALS AND METHODS: In this study, we genotyped 217 individuals, 99 patients with renal cell carcinoma (RCC) and 118 controls without cancer. DNA specimens were extracted from epithelial buccal cells and paraffin-embedded tissue of RCC patients and from epithelial buccal cells and blood cells of healthy controls RESULTS: The difference in frequency of 2G/2G genotype between controls (22.9%) and RCC patients (28.6%) was not statistically significant (p = 0.461). We also did not find correlation between 2G/2G and histological type of RCC. The comparison of genotype distribution and frequency of 2G allele in different populations showed a strong variability of 2G allele frequency among the different ethnic groups. This fact may influence on the collaboration of this 2G allele in RCC or others diseases CONCLUSION: Our data suggest that the matrix metalloproteinase-1 (MMP-1) promoter polymorphism may not play a significant role in renal cell carcinoma patients in Brazil.

‣ In vitro evidence for a new therapeutic approach in renal cell carcinoma

Pittoggi,Carmine; Martis,Gianni; Mastrangeli,Giorgia; Mastrangeli,Bruno; Spadafora,Corrado
Fonte: Sociedade Brasileira de Urologia Publicador: Sociedade Brasileira de Urologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/08/2008 Português
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PURPOSE: Renal cell carcinoma (RCC) is the most lethal among the common urologic malignancies, comprising 3% of all human neoplasias; approximately 40% of patients eventually die of cancer progression. One third of patients who present with metastatic disease and up to 40% treated for localized disease generally experience recurrence. RCCs are characterized by high resistance to chemo-, radio- and immunotherapy. We recently discovered an endogenous enzymatic activity, which is particularly expressed in tumorigenic cell, endogenous non-telomerase reverse transcriptase (RT) of retrotrasposon / retroviral origin, as a specific target to induce proliferation arrest in a number of human carcinogenesis in vitro culture cell lines. METHODS: To address this possibility, we have employed RCC primary cell culture testing pharmacological inhibition, in vitro, by two characterized non nucleosidic RT inhibitors, nevirapine and efavirenz; next, we assessed morphological effects and analyzed putative modulation on gene expression profile. RESULTS: Both treatments reduced cell proliferation rate and induced morphological differentiation and gene expression reprogramming in different RCC analyzed tumor biomarkers. CONCLUSION: In this study we describe a new potential therapeutic approach to obtain considerable future benefits in renal carcinoma cure and attempt to establish a new possible pharmacological therapy based on oral drugs administration in renal RCC treatment.

‣ Localized renal cell carcinoma management: an update

Heldwein,Flavio L.; Mccullough,T. Casey; Souto,Carlos A. V.; Galiano,Marc; Barret,Eric
Fonte: Sociedade Brasileira de Urologia Publicador: Sociedade Brasileira de Urologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/12/2008 Português
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OBJECTIVE: To review the current modalities of treatment for localized renal cell carcinoma. MATERIALS AND METHODS: A literature search for keywords: renal cell carcinoma, radical nephrectomy, nephron sparing surgery, minimally invasive surgery, and cryoablation was performed for the years 2000 through 2008. The most relevant publications were examined. RESULTS: New epidemiologic data and current treatment of renal cancer were covered. Concerning the treatment of clinically localized disease, the literature supports the standardization of partial nephrectomy and laparoscopic approaches as therapeutic options with better functional results and oncologic success comparable to standard radical resection. Promising initial results are now available for minimally invasive therapies, such as cryotherapy and radiofrequency ablation. Active surveillance has been reported with acceptable results, including for those who are poor surgical candidates. CONCLUSIONS: This review covers current advances in radical and conservative treatments of localized kidney cancer. The current status of nephron-sparing surgery, ablative therapies, and active surveillance based on natural history has resulted in great progress in the management of localized renal cell carcinoma.

‣ Metastatic renal cell carcinoma management

Heldwein,Flavio L.; Escudier,Bernard; Smyth,Gordon; Souto,Carlos A. V.; Vallancien,Guy
Fonte: Sociedade Brasileira de Urologia Publicador: Sociedade Brasileira de Urologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/06/2009 Português
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PURPOSE: To assess the current treatment of metastatic renal cell carcinoma, focusing on medical treatment options. MATERIAL AND METHODS: The most important recent publications have been selected after a literature search employing PubMed using the search terms: advanced and metastatic renal cell carcinoma, anti-angiogenesis drugs and systemic therapy; also significant meeting abstracts were consulted. RESULTS: Progress in understanding the molecular basis of renal cell carcinoma, especially related to genetics and angiogenesis, has been achieved mainly through of the study of von Hippel-Lindau disease. A great variety of active agents have been developed and tested in metastatic renal cell carcinoma (mRCC) patients. New specific molecular therapies in metastatic disease are discussed. Sunitinib, Sorafenib and Bevacizumab increase the progression-free survival when compared to therapy with cytokines. Temsirolimus increases overall survival in high-risk patients. Growth factors and regulatory enzymes, such as carbonic anhydrase IX may be targets for future therapies. CONCLUSIONS: A broader knowledge of clear cell carcinoma molecular biology has permitted the beginning of a new era in mRCC therapy. Benefits of these novel agents in terms of progression-free and overall survival have been observed in patients with mRCC...

‣ Epidemiologic characteristics of renal cell carcinoma in Brazil

Nardi,Aguinaldo C.; Zequi,Stenio de C.; Clark,Otavio A. C.; Almeida,Jose C.; Glina,Sidney
Fonte: Sociedade Brasileira de Urologia Publicador: Sociedade Brasileira de Urologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/04/2010 Português
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PURPOSE: In Brazil, National data regarding the epidemiology of renal cell carcinoma (RCC) are scarce. The aim of this study was to describe the demographic, clinical, and pathologic characteristics of RCC diagnosed and treated by members of the SBU - Brazilian Society of Urology. MATERIALS AND METHODS: For this cross-sectional study, data were collected through an on line questionnaire available to the members of the Brazilian Society of Urology (SBU). Between May 2007 and May 2008, voluntary participant urologists collected data on demographic, clinical and pathological characteristics from patients diagnosed with RCC in their practice. RESULTS: Fifty SBU affiliated institutions contributed with patient information to the study. Of the 508 patients, 58.9% were male, 78.9% were white, and the mean age was 59.8 years. Smoking history, high blood pressure and a body mass index above 30 kg/m2 were present in 14.8%, 46.1% and 17.9% of the patients, respectively. Abdominal ultrasound and computed tomography were the main diagnostic methods. The majority of the cases were localized tumors and metastasis were presented in 9.5% of the patients; 98.4% underwent nephrectomy. Clear cell carcinoma was the most common histological type. In comparison with private institutions...

‣ SEOM clinical guidelines for the treatment of renal cell carcinoma

Bellmunt, J.; Puente, J.; Garcia de Muro, J.; Lainez, N.; Rodríguez, C.; Duran, I.
Fonte: Springer Milan Publicador: Springer Milan
Tipo: Artigo de Revista Científica
Português
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The purpose of this article was to provide updated recommendations for the diagnosis and treatment of renal cell carcinoma. Pathological confirmation is mandatory before treatment with ablative or focal therapies before any type of systemic therapy. Renal cell cancer should be staged according to the TNM classification system. A laparoscopic nephron-sparing surgery should be the approach for tumors <4 cm if technically feasible. Otherwise, radical (or partial in selected cases) nephrectomy is the treatment of choice, with lymph node dissection only performed in patients with clinically detected lymph node involvement. Some retrospective evidence for a cytoreductive nephrectomy in the postimmunotherapy era suggests a benefit in patients with good or intermediate risk or for patients with a symptomatic primary lesion. Adjuvant treatment with chemotherapy or with targeted agents is not recommended and studies are ongoing today. Patients with metastatic disease should be staged by computed tomography scans of the chest, abdomen and pelvis. The efficacy of sunitinib, bevacizumab plus interferon-α, and pazopanib is well established in patients with good and intermediate risk as well for temsirolimus in poor-risk patients. These four agents are considered standard of care in first-line treatment. Sorafenib...

‣ HLA-Ligandom-, Genexpressions- und T-Zell-Analysen zur Identifizierung tumorassoziierter Peptide des Nierenzellkarzinoms und der Akuten Myeloischen Leukämie; HLA ligandome, gene expression and T-cell studies to identify tumor associated peptides in renal cell carcinoma and acute myeloid leukaemia

Krüger, Tobias
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
997.2855%
Immuntherapeutische Strategien sind vielversprechende Ansätze in der Therapie maligner Erkrankungen. Schlüsselstruktur für die Erkennung maligner Zellen durch die T-Zellen des Immunsystems sind tumorassoziierte, HLA-präsentierte Peptide. Zielsetzung der vorliegenden Arbeit war es, das HLA-Ligandom neoplastischer Zellen durch massenspektrometrische (LC/MS) Peptidsequenzierung näher zu charakterisieren, sowie mit Hilfe der Microarray-basierten Genexpressionsanalyse solche Gene bzw. Proteine zu identifizieren, die aufgrund ihres Expressionsmusters als tumorassoziiert anzusehen sind. Analysiert wurden solide Nierenzellkarzinome (RCC) sowie Zelllinien der Akuten Myeloischen Leukämie (AML) und des RCC. Insgesamt konnten 725 verschiedene Peptidsequenzen aus 11 soliden RCCs und 204 Peptide aus einer RCC-Zelllinie gewonnen werden. Die meisten dieser HLA-Liganden entsprachen sog. "Selbstpeptiden", für die sich keine Hinweise auf eine Tumorassoziation ergaben. Jedoch konnten sowohl in den soliden Tumoren als auch in der Zelllinie zahlreiche neue Peptide aus bereits etablierten tumorassoziierten Proteinen (MET, ADFP, CA9, CCND1, RPL10A) sowie aus Proteinen, deren korrespondierende Genexpression eine Tumorassoziation nahe legt (MMP7, APOL1...

‣ Immunhistologische Analyse von Zelladhäsions- und Matrixmolekülen bei Nierenzellkarzinomen; Immunohistological analysis of cell adhesion and matrix molecules on renal cell carcinoma

Widmann, Susanne
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
1013.56875%
In dieser Arbeit wurde untersucht, ob bei renalen Karzinomen ein pathologisch verändertes Expressionsmuster von Adhäsionsmolekülen in Abhängigkeit vom Tumorstadium, vom zellulären Malignitätsgrad und der Histologie aufgezeigt werden kann. E- und N-Cadherin, die in der normalen Niere im Bereich distaler Tubulusanteile und der Sammelrohre bzw. auf Zellen des proximalen Tubulusepithels selektiv zu finden sind, wurden im Tumorgewebe in den meisten Fällen gleichzeitig exprimiert. Drei Tumore waren für beide Moleküle negativ. Bei einigen entdifferenzierten Tumoren wechselte die Expression von einer umschriebenen Verteilung auf der Zelloberfläche zu einer diffusen Anfärbung auch des Zytoplasmas. Die Mehrzahl der NZK zeigte keine VE-Cadherin-Expression. VE-Cadherin konnte im normalen Nierengewebe auf Endothelzellen verschiedener Blutgefäße, interstitieller Kapillaren und Glomeruli, im Tumorgewebe nur im Bereich von fokal gruppierten Gefäßen detektiert werden. Die meisten Endothelien waren in den NZK vermutlich als Hinweis für ihre geringe Ausreifung für VE-Cadherin negativ. P-Cadherin mit einer beschränkten Expression auf wenigen proximalen Tubuluszellen, mesangialen Zellen in Glomeruli und auf der Intima größerer Gefäße in der Normalniere...

‣ Identifikation MHC-I-gebundener Liganden tumorassoziierter Antigene zur Optimierung einer individualisierten Vakzinierungstherapie des Nierenzellkarzinoms und kolorektalen Karzinoms; Identification of MHC-I-ligands, derived from tumor associated antigens, to optimize vaccination therapy for renal cell carcinoma and colorectal carcinoma

Häntschel, Maik
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
1002.36695%
Nierenzell- und kolorektale Karzinome zählen zu den führenden Malignomentitäten der westlichen Welt und stellen bei schlechter Therapierbarkeit und hoher Mortalität für den einzelnen Menschen eine ernsthafte Bedrohung, für die Gesellschaft eine relevante Belastung durch Ausfall an Arbeitskraft und steigende Kosten dar. Für beide Tumoren wurde mit der Vakzinierungstherapie ein neuer Behandlungsansatz durch Utilisation des eigenen Immunsystems verfolgt. Die vorliegende Arbeit baut auf diesem Prinzip auf. Nach Asservierung und allgemeiner bzw. spezieller Charakterisierung von über 50 nativen Tumorgeweben dieser Entitäten wurden über eine Säureextraktion nach Immunpräzipitation MHC- I- präsentierte Liganden der Tumorzelloberfläche isoliert und – mit Hilfe der jeweiligen HLA- Motive – massenspektrometrisch ihre Sequenz bestimmt. Insgesamt konnten 70 Liganden auf verschiedenen Tumorgeweben detektiert werden. Während ein Teil als vorbeschrieben bestätigt werden konnte, ließen sich andere, bekannte Peptide erstmalig auf diesen Entitäten nachweisen. Das Gros dieser Peptide war jedoch noch nie zuvor als HLA- Ligand identifiziert worden und konnte damit erstmalig beschrieben werden. Drei dieser bislang unbekannten Peptide entstammen Proteinen...

‣ Korrelation der Bone Sialoprotein-Expression mit dem Metastasierungsgrad von Nierenzellkarzinomen - eine immunhistochemische Analyse; Correlation of the expression of Bone Sialoprotein in renal cell carcinoma subject to its metastatic disease - an immunohistochemical analysis

Minkley, Lena
Fonte: Universidade de Tubinga Publicador: Universidade de Tubinga
Tipo: Dissertação
Português
Relevância na Pesquisa
1000.8785%
Einleitung: Bone Sialoprotein (BSP) ist Bestandteil mineralisierter Gewebe wie Knochen, Cementum, Dentin und kalzifiziertem Knorpel und stellt einen wesentlichen Teil der Extrazellularmatrix dar. BSP steuert den Knochenstoffwechsel indem es direkt die Osteoblasten- und Osteoklastenaktivität beeinflusst. Neuerdings ist gezeigt worden, dass BSP in einer Vielzahl weiterer Gewebe exprimiert wird. Für Mamma- und - Bronchialkarzinome konnte ein signifikanter Zusammenhang zwischen der BSP-Gewebeexpression der Tumoren und dem Auftreten von Knochenmetastasen gezeigt werden. Die potenzielle Rolle von BSP für die ossäre Metastasierung des Nierenzellkarzinoms (NZK) ist noch nicht untersucht. Ziel dieser Studie war, die BSP-Expression beim NZK in Abhängigkeit von dessen Metastasierungscharakter zu bestimmen. Methoden: Untersucht wurden Gewebeproben von jeweils 10 Patienten mit operativ entfernten NZK, die zum Operationszeitpunkt keinerlei Metastasen (Gruppe I, 3x T<3, 7x T3), nur Weichteilmetastasen (II, 2/8) oder zusätzlich Knochenmetastasen (III, 3/7) aufwiesen. Immunhistochemisch wurde die BSP-Expression in den jeweiligen NZK und korrespondierenden Nierenparenchymen bestimmt. Die Auswertung erfolgte anhand eines etablierten semiquantitativen Scoring-Systems. Expressionen wurden als relative Anteile des Maximums dargestellt und mittels One way ANOVA nach Gruppenzugehörigkeit und klinischen Daten verglichen. Zur Prüfung eines Zusammenhangs zwischen Tumorgeweben und Nierengeweben wurden lineare Regressionsanalysen durchgeführt. Ergebnisse: Sowohl im NZK als auch im Nierengewebe konnte BSP immunhistochemisch nachgewiesen werden. Die NZK zeigten dabei keine nennenswerten Unterschiede der Expressionen zwischen den 3 Gruppen (200...

‣ Increased expression of 5-lipoxygenase is common in clear cell renal cell carcinoma

Faronato, M.; Muzzonigro, G.; Milanese, G.; Menna, C.; Bonfigli, A.R.; Catalano, A.; Procopio, A.
Fonte: Murcia : F. Hernández Publicador: Murcia : F. Hernández
Tipo: Artigo de Revista Científica Formato: application/pdf
Português
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The clinical behaviour of Clear Cell Renal Cell Carcinoma (CC-RCC) is often unpredictable. To fully understand the signaling pathways involved in CCRCC development, we examined whether the 5- Lipoxygenase (5-LO), which catalyzes the biosynthesis of proinflammatory leukotrienes, is involved in renal tumorigenesis. By analyzing 46 snap-frozen primary renal cell carcinomas and their corresponding normal renal cortex biopsies, 5-LO protein levels were found to be significantly increased in the majority of CC-RCCs (P<0.001). Quantitative 5-LO mRNA expression analysis revealed up to 3-fold increased expression in the tumor tissues. There was no association between 5-LO and gender, grade or vein invasion. In contrast, increased 5-LO protein and mRNA correlated with large tumor size (>4 cm) and age of patients (P<0.001). 5-LO was frequently overexpressed in von Hippel-Lindau protein (pVHL)–reduced tumors and in Vascular Endothelial Growth Factor (VEGF)-positive tumors, which represent two frequent alterations in CC-RCC. Cell culture experiments demonstrated that VEGF expression was strongly inducible by 5-LO metabolites in RCC cell lines. The loss of pVHL expression led to high basal 5- LO and VEGF expression, which were markedly reduced by transfection with 5-LO small interfering RNA (siRNA). These results suggest that 5-LO upregulation is an important step in renal cancer progression.

‣ Prognostic relevance of the histological subtype of renal cell carcinoma

DALL'OGLIO, Marcos F.; ANTUNES, Alberto A.; POMPEO, Antonio C.; MOSCONI, Alcides; LEITE, Katia R. M.; Srougi, Miguel
Fonte: Sociedade Brasileira de Urologia Publicador: Sociedade Brasileira de Urologia
Tipo: Artigo de Revista Científica
Português
Relevância na Pesquisa
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OBJECTIVE: According to several studies, when the histological subtype of renal cell carcinoma is established it is possible to attribute a different life expectancy to each patient. We analyzed the prognostic significance of the histological subtype in renal cell carcinoma. MATERIALS AND METHODS: The authors retrospectively analyzed the follow-up of 230 patients after radical or conservative renal surgery. The histological characteristics of the different subtypes of tumor were obtained and the disease-free and cancer-specific survival curves for the clear cell, cromophobic, papillary, collecting duct (Bellini) subtypes and those with sarcomatoid differentiation were individualized. RESULTS: The disease-free and cancer-specific survival rates for clear cell tumors were 76.6% and 68.0% respectively, 71.2% and 82.1% respectively for the cromophobic type, 71.1% and 79.8% respectively for the papillary type, 26.9% and 39.3% respectively for the sarcomatoid type, and 0.0% and 0.0% respectively for the collecting ducts (p < 0.001). CONCLUSION: The histological subtypes of renal tumors can stratify patients into different prognostic groups only when the sarcomatoid differentiation is present.

‣ Estudo de fatores prognósticos moleculares no carcinoma renal de células claras pela técnica de tissue microarray; Study of molecular prognostic factors in clear cell renal cell carcinoma by tissue microarray

Zerati, Marcelo
Fonte: Biblioteca Digitais de Teses e Dissertações da USP Publicador: Biblioteca Digitais de Teses e Dissertações da USP
Tipo: Tese de Doutorado Formato: application/pdf
Publicado em 01/08/2011 Português
Relevância na Pesquisa
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INTODUÇÃO: O carcinoma renal (CR) é uma doença agressiva, e sua incidência vem aumentando. A variante de células claras (CRCC) é a mais comum e apresenta comportamento biológico mais agressivo. Os recentes avanços no conhecimento da biologia molecular do tumor demonstram que a oncogênese dos diversos tipos histológicos é regida por mecanismos celulares diversos. Os modelos prognósticos atuais vêm procurando incorporar os recentes avanços da biologia molecular, com o intuito de melhorar sua capacidade de predizer a evolução e o desfecho destes pacientes. OBJETIVOS: Correlacionar a imunoexpressão dos marcadores selecionados com: 1) sobrevida global e, 2) com parâmetros prognósticos estabelecidos (estadio clínico TNM, tamanho tumoral, grau nuclear de Fuhrman, invasão microvascular e invasão de gordura perirrenal) em portadores de CRCC não metastático. MÉTODOS: Neste estudo de coorte retrospectivo, avaliamos 99 pacientes portadores de CRCC não metastático, quanto à expressão imunoistoquímica das seguintes proteínas: CA-IX, EGF-R, Ki-67, p53, PTEN, VEGF e VEGF-R. Os parâmetros analisados foram: Sobrevida global, estadio TNM, tamanho tumoral, grau nuclear de Fuhrman, invasão microvascular e invasão de gordura perirrenal. Utilizamos um tissue microarray construído exclusivamente para esta finalidade e realizamos a leitura da imunoexpressão por técnica digital utilizando o software Photoshop®. RESULTADOS: O tempo de seguimento médio foi de 7...

‣ Prognostic relevance of the histological subtype of renal cell carcinoma

Dall'Oglio,Marcos F.; Antunes,Alberto A.; Pompeo,Antonio C.; Mosconi,Alcides; Leite,Katia R. M.; Srougi,Miguel
Fonte: Sociedade Brasileira de Urologia Publicador: Sociedade Brasileira de Urologia
Tipo: Artigo de Revista Científica Formato: text/html
Publicado em 01/02/2008 Português
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OBJECTIVE: According to several studies, when the histological subtype of renal cell carcinoma is established it is possible to attribute a different life expectancy to each patient. We analyzed the prognostic significance of the histological subtype in renal cell carcinoma. MATERIALS AND METHODS: The authors retrospectively analyzed the follow-up of 230 patients after radical or conservative renal surgery. The histological characteristics of the different subtypes of tumor were obtained and the disease-free and cancer-specific survival curves for the clear cell, cromophobic, papillary, collecting duct (Bellini) subtypes and those with sarcomatoid differentiation were individualized. RESULTS: The disease-free and cancer-specific survival rates for clear cell tumors were 76.6% and 68.0% respectively, 71.2% and 82.1% respectively for the cromophobic type, 71.1% and 79.8% respectively for the papillary type, 26.9% and 39.3% respectively for the sarcomatoid type, and 0.0% and 0.0% respectively for the collecting ducts (p < 0.001). CONCLUSION: The histological subtypes of renal tumors can stratify patients into different prognostic groups only when the sarcomatoid differentiation is present.

‣ Extremely delayed renal cell carcinoma metastasis mimicking convexity meningioma

Bademci,G.; Bozdogan,O.; Berdan,F.; Evliyaoglu,C.
Fonte: Neurocirugía Publicador: Neurocirugía
Tipo: info:eu-repo/semantics/article; journal article; info:eu-repo/semantics/publishedVersion Formato: text/html; application/pdf
Publicado em 01/12/2008 Português
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Cerebral extra-axial metastasis mimicking meningioma which satisfy several criteria for a diagnosis of meningioma, but which have proved instead to be metastatic carcinoma and extremely delayed cerebral metastasis from renal cell carcinoma form the focus of the presentation. 68-year-old-woman who had been operated for renal cell carcinoma 20 years previously is presented with new symptoms of intracranial mass. A large extraaxial mass of the convexity which destroyed calvarium and dura was excised with Simpson Grade I removal, revealed metastatic carcinoma. Imaging characteristics can not always discern between meningioma and metastatic tumours. A meticulous clinical evaluation and histopathological diagnosis is essential in patients with intracranial mass even they resemble both primary and metastatic tumours.